本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信...本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。展开更多
Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the correspondi...Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the corresponding (four-dimensional) real Lie groups endowed with bi-invariant metrics of Lorentzian signature. Similar contractions of (seven-dimensional) isometry Lie algebras iso(D), iso(F) to iso(L) are determined. The group SU(2,2) acts on each of the D, L, F by conformal transformation which is a core feature of the DLF-theory. Also, d and f are contracted to T, S-abelian subalgebras, generating parallel translations, T, and proper conformal transformations, S (from the decomposition of su(2,2) as a graded algebra T + Ω + S, where Ω is the extended Lorentz Lie algebra of dimension 7).展开更多
The present study investigates the wavespace of Highly Contrasted Structures(HCS)and Highly Dissipative Structures(HDS)by wave-based models.The Asymptotic Homogenization Method(AHM),exploits the asymptotic Zig-Zag mod...The present study investigates the wavespace of Highly Contrasted Structures(HCS)and Highly Dissipative Structures(HDS)by wave-based models.The Asymptotic Homogenization Method(AHM),exploits the asymptotic Zig-Zag model and homogenization technique to compute the bending wavenumbers via a 6th-order equation.The General Laminate Model(GLM)employs Mindlin’s displacement field to establish displacement-constraint relationships and resolves a quadratic Eigenvalue Problem(EVP)of the dispersion relation.The Wave Finite Element(WFE)scheme formulates the Nonlinear Eigenvalue Problem(NEP)for waves in varying directions and tracks complex wavenumbers using Weighted Wave Assurance Criteria(WWAC).Two approaches are introduced to estimate the Damping Loss Factor(DLF)of HDS,with the average DLF calculated by the modal density at various angles where non-homogeneity is present.Evaluation of robustness and accuracy is made by comparing the wavenumbers and DLF obtained from AHM and GLM with WFE.WFE is finally extended to a sandwich metastructure with a non-homogeneous core,and the Power Input Method(PIM)with Finite Element Method(FEM)data is employed to assess the average DLF,demonstrating an enhanced DLF compared to layered configurations with the same material portion,indicating increased energy dissipation due to the bending-shear coupling effects.展开更多
文摘本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。
文摘Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the corresponding (four-dimensional) real Lie groups endowed with bi-invariant metrics of Lorentzian signature. Similar contractions of (seven-dimensional) isometry Lie algebras iso(D), iso(F) to iso(L) are determined. The group SU(2,2) acts on each of the D, L, F by conformal transformation which is a core feature of the DLF-theory. Also, d and f are contracted to T, S-abelian subalgebras, generating parallel translations, T, and proper conformal transformations, S (from the decomposition of su(2,2) as a graded algebra T + Ω + S, where Ω is the extended Lorentz Lie algebra of dimension 7).
基金supported by the Natural Sciences and Engineering Research Council of Canada-Discovery Grant(individual)Program(No.NSEC-DG#355433-2009)funded by the LabEx CeLyA(Centre Lyonnais d’Acoustique,No.ANR-10-LABX-0060)of Universite?de Lyon。
文摘The present study investigates the wavespace of Highly Contrasted Structures(HCS)and Highly Dissipative Structures(HDS)by wave-based models.The Asymptotic Homogenization Method(AHM),exploits the asymptotic Zig-Zag model and homogenization technique to compute the bending wavenumbers via a 6th-order equation.The General Laminate Model(GLM)employs Mindlin’s displacement field to establish displacement-constraint relationships and resolves a quadratic Eigenvalue Problem(EVP)of the dispersion relation.The Wave Finite Element(WFE)scheme formulates the Nonlinear Eigenvalue Problem(NEP)for waves in varying directions and tracks complex wavenumbers using Weighted Wave Assurance Criteria(WWAC).Two approaches are introduced to estimate the Damping Loss Factor(DLF)of HDS,with the average DLF calculated by the modal density at various angles where non-homogeneity is present.Evaluation of robustness and accuracy is made by comparing the wavenumbers and DLF obtained from AHM and GLM with WFE.WFE is finally extended to a sandwich metastructure with a non-homogeneous core,and the Power Input Method(PIM)with Finite Element Method(FEM)data is employed to assess the average DLF,demonstrating an enhanced DLF compared to layered configurations with the same material portion,indicating increased energy dissipation due to the bending-shear coupling effects.
