To explore the mechanism of sperm dysfunction caused by dibutyl phthalate(DBP),the effects of DBP on intracellular[Ca^(2+)]and[pH],reactive oxygen species(ROS),lipid peroxidation(LPO),mitochondrial permeability transi...To explore the mechanism of sperm dysfunction caused by dibutyl phthalate(DBP),the effects of DBP on intracellular[Ca^(2+)]and[pH],reactive oxygen species(ROS),lipid peroxidation(LPO),mitochondrial permeability transition pore(mPTP)opening,mitochondrial membrane potential(MMP),adenosine triphosphate(ATP)levels,phosphorylation of protein kinase A(PKA)substrate proteins and phosphotyrosine(p-Tyr)proteins,sperm motility,spontaneous acrosome reaction,and tail bending were examined in mouse spermatozoa.At 100μg/mL,DBP significantly increased tail bending and[Ca^(2+)]i.Interestingly,DBP showed biphasic effects on[pH]i.DBP at 10–100μg/mL significantly decreased sperm motility.Similarly,Ca^(2+)ionophore A23187 decreased[pH]_(i)sperm motility,suggesting that DBP-induced excessive[Ca^(2+)]_(i)decreased sperm motility.DBP significantly increased ROS and LPO.DBP at 100μg/mL significantly decreased mPTP closing,MMP,and ATP levels in spermatozoa,as did H2O2,indicative of ROS-mediatedmitochondrial dysfunction caused by DBP.DBP as well as H2O2 increased p-Tyr sperm proteins and phosphorylated PKA substrate sperm proteins.DBP at 1–10μg/mL significantly increased the spontaneous acrosome reaction,suggesting that DBP can activate sperm capacitation.Altogether,DBP showed a biphasic effect on intracellular signaling in spermatozoa.At concentrations relevant to seminal ortho-phthalate levels,DBP activates[pH]i,protein tyrosine kinases and PKA via physiological levels of ROS generation,potentiating sperm capacitation.DBP at high doses excessively raises[Ca^(2+)]_(i)and ROS and disrupts[pH]i,impairing the mitochondrial function,tail structural integrity,and sperm motility.展开更多
The hydrophobic flocculation of marmatite fines in aqueous suspensions induced by butyl xanthate （KBX） and ammonium dibutyl dithiophosphate （ADD） was investigated using laser particle size analysis, microscopy ana...The hydrophobic flocculation of marmatite fines in aqueous suspensions induced by butyl xanthate （KBX） and ammonium dibutyl dithiophosphate （ADD） was investigated using laser particle size analysis, microscopy analysis, electrophoretic light scattering, contact angle measurement and infrared spectroscopy. The studies were performed on single minerals with size 〈20 μm by varying several parameters, including pH, collector concentration and kerosene addition. The results show that the floc fotation closely correlated with the size of flocs and the particle hydrophobicity, but was not lowered with increasing the particle surface charges due to collector adsorption. Under good operating conditions, the floc flotation of marmatite fines as a function of KBX and ADD can all reach floatability over 90%, in comparison with conventional flotation obtaining floatability of about 60%. It also has been found that a small addition of kerosene greatly improved the floc flotation because of the formation of oil films on marmatite particles. The results of FTIR spectra indicate that adsorption of the two collectors onto marmatite were chemical adsorption.展开更多
Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, how...Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, however, whether such early gestational and/or lactational exposure can influence the later adult-type Leydig cell phenotype. In this study, Sprague-Dawley rats were exposed to dibutyl phthalate （DBP; from gestational day （GD） 14.5 to postnatal day （PND） 6） or diethylstilbestrol （DES; from GD14o5 to GD16.5） during a short gestationalllactational window, and male offspring subsequently analysed for various postnatal testicular parameters. All offspring remained in good health throughout the study. Maternal xenobiotic treatment appeared to modify specific Leydig cell gene expression in male offspring, particularly during the dynamic phase of mid-puberty, with serum INSL3 concentrations showing that these compounds led to a faster attainment of peak values, and a modest acceleration of the pubertal trajectory. Part of this effect appeared to be due to a treatment-specific impact on Leydig cell proliferation during puberty for both xenobiotics. Taken together, these results support the notion that maternal exposure to certain xenobiotics can also influence the development of the adult-type Leydig cell population, possibly through an effect on the Leydig stem cell population.展开更多
Objective To evaluate the combined subchronic toxicity of bisphenol A（BPA） and dibutyl phthalate（DBP） in male Sprague Dawley（SD） rats.Methods Forty 4‐week‐old male rats weighing 115‐125 g were randomly divide...Objective To evaluate the combined subchronic toxicity of bisphenol A（BPA） and dibutyl phthalate（DBP） in male Sprague Dawley（SD） rats.Methods Forty 4‐week‐old male rats weighing 115‐125 g were randomly divided into BPA‐treated,DBP‐treated group,BPA＋DBP‐treated and control groups and fed with a soy‐ and alfalfa‐free diet containing 285.4 ppm BPA,285.4 ppm DBP,285.4 ppm BPA plus 285.4 ppm DBP,and a control diet,respectively,for 90 consecutive days.At the end of the study,the animals were sacrificed by exsanguination via the carotid artery under diethyl etherane aesthesia and weighed.Organs,including liver,kidneys,spleen,thymus,heart,brain,and testis underwent pathological examination.The androgen receptor（AR）,gonadotropin‐releasing hormone receptor（GNRHR）,and progesterone hormone receptor（PR） genes from the hypothalamus were detected by real‐time PCR.The biomedical parameters were analyzed.Results No significant difference was found in food intake,body weight,tissue weight,organ/brain weight ratio,and biomedical parameters among the four groups（P〉0.05）.However,BPA and DBP up‐regulated AR,PR and GNRHR expression levels in rats and showed a synergistic or an additive effect in the BPA＋DBP group.Conclusion The combined subchronic toxicity of BPA and DBP is synergistic or additive in male SD rats.展开更多
The dibutyl phthalate （DBP） concentration in liqueur was measured by gas chromatography-mass spectrometry （GC-MS）, and the uncertainty during the mea-surement was evaluated in this study. The results showed that t...The dibutyl phthalate （DBP） concentration in liqueur was measured by gas chromatography-mass spectrometry （GC-MS）, and the uncertainty during the mea-surement was evaluated in this study. The results showed that the combined stan-dard uncertainty was determined as 0.028 and the expanded uncertainty was 0.056 at confidence probability p=95%, coverage factor k=2, by fol owing the methods de-scribed in GB/T 21911-2008 ＂Determination of Phthalate Esters in Foods＂. The av-erage DBP concentration in the liqueur of eight repeated measurements was（0.985＆#177; 0.056） mg/kg finaly.展开更多
The hydrophobic flocculation of jamesonite fines in aqueous suspensions induced by ammonium dibutyl dithiophosphate was investigated using laser particle size analysis, microscope analysis, electrophoretic light scatt...The hydrophobic flocculation of jamesonite fines in aqueous suspensions induced by ammonium dibutyl dithiophosphate was investigated using laser particle size analysis, microscope analysis, electrophoretic light scattering and infrared spectroscopy.Single minerals of 4.607 μm for the 50% volumetric diameters were researched by varying several parameters, including p H,ammonium dibutyl dithiophosphate concentration, stirring strength and kerosene addition. It is found that the maximal floatability of jamesonite fines is induced by ammonium dibutyl dithiophosphate at p H 6, and the floc flotation increases with increasing ammonium dibutyl dithiophosphate concentration despite a simultaneous increase in the negative ζ potential of jamesonite, meaning that hydrophobic interaction between the particles increases much more strongly than electric double layer repulsion from the adsorption of ammonium dibutyl dithiophosphate. It is also found that the floc flotation is closely correlated with the size of flocs,which is strongly influenced by the stirring strength and enhanced by the addition of a small amount of kerosene. The results of FTIR spectra indicate that adsorption of ammonium dibutyl dithiophosphate onto jamesonite is chemical adsorption and the adsorption product is lead dibutyl dithiophosphate.展开更多
In recent years,the presence and adverse impacts of phthalic acid esters m aquatic environments have gained increasing attention.This work investigated the adsorption behavior of a typical phthalic acid ester,dibutyl ...In recent years,the presence and adverse impacts of phthalic acid esters m aquatic environments have gained increasing attention.This work investigated the adsorption behavior of a typical phthalic acid ester,dibutyl phthalate(DBP),onto steel slag,gravel,and shell sand(substrates commonly used in constructed wetlands).The influence of dissolved organic matter(DOM)on DBP adsorption was investigated using humic acid as a proxy for DOM.The results demonstrated that the adsorption of DBP to three substrates reached equilibrium within 96 h,and the adsorption kinetics were well fitted by a pseudo-second-order model.The DBP adsorption isotherms were best fitted by the Langmuir adsorption model.The DBP adsorption capacity decreased in the order of steel slag>gravel>sheII sand,with values of 656 mg/kg,598 mg/kg,and 6.62 mg/kg at 25°C,respectively.DBP adsorbed to the surface of all substrates in a monolayer via an endothermic process.The DBP adsorption capacities of steel slag and gravel decreased as the DOM content increased.The DBP adsorption mechanisms to steel slag and gravel mainly involved the surface coordination of DBP with-OH or-COOH groups and electrostatic interactions.The results of this work suggest that steel slag and gravel may be ideal substrates for use in constructed wetlands to treat wastewater polluted with DBP.展开更多
基金supported by the National Research Foundation of Republic of Korea(NRF)grant funded by the Republic of Korea government(MSIT)(No.2022R1A2C1007831).
文摘To explore the mechanism of sperm dysfunction caused by dibutyl phthalate(DBP),the effects of DBP on intracellular[Ca^(2+)]and[pH],reactive oxygen species(ROS),lipid peroxidation(LPO),mitochondrial permeability transition pore(mPTP)opening,mitochondrial membrane potential(MMP),adenosine triphosphate(ATP)levels,phosphorylation of protein kinase A(PKA)substrate proteins and phosphotyrosine(p-Tyr)proteins,sperm motility,spontaneous acrosome reaction,and tail bending were examined in mouse spermatozoa.At 100μg/mL,DBP significantly increased tail bending and[Ca^(2+)]i.Interestingly,DBP showed biphasic effects on[pH]i.DBP at 10–100μg/mL significantly decreased sperm motility.Similarly,Ca^(2+)ionophore A23187 decreased[pH]_(i)sperm motility,suggesting that DBP-induced excessive[Ca^(2+)]_(i)decreased sperm motility.DBP significantly increased ROS and LPO.DBP at 100μg/mL significantly decreased mPTP closing,MMP,and ATP levels in spermatozoa,as did H2O2,indicative of ROS-mediatedmitochondrial dysfunction caused by DBP.DBP as well as H2O2 increased p-Tyr sperm proteins and phosphorylated PKA substrate sperm proteins.DBP at 1–10μg/mL significantly increased the spontaneous acrosome reaction,suggesting that DBP can activate sperm capacitation.Altogether,DBP showed a biphasic effect on intracellular signaling in spermatozoa.At concentrations relevant to seminal ortho-phthalate levels,DBP activates[pH]i,protein tyrosine kinases and PKA via physiological levels of ROS generation,potentiating sperm capacitation.DBP at high doses excessively raises[Ca^(2+)]_(i)and ROS and disrupts[pH]i,impairing the mitochondrial function,tail structural integrity,and sperm motility.
基金Project(51274255)supported by the National Natural Science Foundation of ChinaProject supported by the Foundation of State Key Laboratory of Comprehensive Utilization of Low-Grade Ores(Zijin Mining Group Co.,Ltd),China
文摘The hydrophobic flocculation of marmatite fines in aqueous suspensions induced by butyl xanthate （KBX） and ammonium dibutyl dithiophosphate （ADD） was investigated using laser particle size analysis, microscopy analysis, electrophoretic light scattering, contact angle measurement and infrared spectroscopy. The studies were performed on single minerals with size 〈20 μm by varying several parameters, including pH, collector concentration and kerosene addition. The results show that the floc fotation closely correlated with the size of flocs and the particle hydrophobicity, but was not lowered with increasing the particle surface charges due to collector adsorption. Under good operating conditions, the floc flotation of marmatite fines as a function of KBX and ADD can all reach floatability over 90%, in comparison with conventional flotation obtaining floatability of about 60%. It also has been found that a small addition of kerosene greatly improved the floc flotation because of the formation of oil films on marmatite particles. The results of FTIR spectra indicate that adsorption of the two collectors onto marmatite were chemical adsorption.
文摘Maternal exposure to estrogenic xenobiotics or phthalates has been implicated in the distortion of early male reproductive development, referred to in humans as the testicular dysgenesis syndrome. It is not known, however, whether such early gestational and/or lactational exposure can influence the later adult-type Leydig cell phenotype. In this study, Sprague-Dawley rats were exposed to dibutyl phthalate （DBP; from gestational day （GD） 14.5 to postnatal day （PND） 6） or diethylstilbestrol （DES; from GD14o5 to GD16.5） during a short gestationalllactational window, and male offspring subsequently analysed for various postnatal testicular parameters. All offspring remained in good health throughout the study. Maternal xenobiotic treatment appeared to modify specific Leydig cell gene expression in male offspring, particularly during the dynamic phase of mid-puberty, with serum INSL3 concentrations showing that these compounds led to a faster attainment of peak values, and a modest acceleration of the pubertal trajectory. Part of this effect appeared to be due to a treatment-specific impact on Leydig cell proliferation during puberty for both xenobiotics. Taken together, these results support the notion that maternal exposure to certain xenobiotics can also influence the development of the adult-type Leydig cell population, possibly through an effect on the Leydig stem cell population.
基金supported by the National Key Technology R&D Program(No.2012BAK01B00)
文摘Objective To evaluate the combined subchronic toxicity of bisphenol A（BPA） and dibutyl phthalate（DBP） in male Sprague Dawley（SD） rats.Methods Forty 4‐week‐old male rats weighing 115‐125 g were randomly divided into BPA‐treated,DBP‐treated group,BPA＋DBP‐treated and control groups and fed with a soy‐ and alfalfa‐free diet containing 285.4 ppm BPA,285.4 ppm DBP,285.4 ppm BPA plus 285.4 ppm DBP,and a control diet,respectively,for 90 consecutive days.At the end of the study,the animals were sacrificed by exsanguination via the carotid artery under diethyl etherane aesthesia and weighed.Organs,including liver,kidneys,spleen,thymus,heart,brain,and testis underwent pathological examination.The androgen receptor（AR）,gonadotropin‐releasing hormone receptor（GNRHR）,and progesterone hormone receptor（PR） genes from the hypothalamus were detected by real‐time PCR.The biomedical parameters were analyzed.Results No significant difference was found in food intake,body weight,tissue weight,organ/brain weight ratio,and biomedical parameters among the four groups（P〉0.05）.However,BPA and DBP up‐regulated AR,PR and GNRHR expression levels in rats and showed a synergistic or an additive effect in the BPA＋DBP group.Conclusion The combined subchronic toxicity of BPA and DBP is synergistic or additive in male SD rats.
文摘The dibutyl phthalate （DBP） concentration in liqueur was measured by gas chromatography-mass spectrometry （GC-MS）, and the uncertainty during the mea-surement was evaluated in this study. The results showed that the combined stan-dard uncertainty was determined as 0.028 and the expanded uncertainty was 0.056 at confidence probability p=95%, coverage factor k=2, by fol owing the methods de-scribed in GB/T 21911-2008 ＂Determination of Phthalate Esters in Foods＂. The av-erage DBP concentration in the liqueur of eight repeated measurements was（0.985＆#177; 0.056） mg/kg finaly.
基金Project(51274255)supported by the National Natural Science Foundation of ChinaProject supported by the Foundation of State Key Laboratory of Comprehensive Utilization of Low-Grade Ores(Zijin Mining Group Co.,Ltd.),China
文摘The hydrophobic flocculation of jamesonite fines in aqueous suspensions induced by ammonium dibutyl dithiophosphate was investigated using laser particle size analysis, microscope analysis, electrophoretic light scattering and infrared spectroscopy.Single minerals of 4.607 μm for the 50% volumetric diameters were researched by varying several parameters, including p H,ammonium dibutyl dithiophosphate concentration, stirring strength and kerosene addition. It is found that the maximal floatability of jamesonite fines is induced by ammonium dibutyl dithiophosphate at p H 6, and the floc flotation increases with increasing ammonium dibutyl dithiophosphate concentration despite a simultaneous increase in the negative ζ potential of jamesonite, meaning that hydrophobic interaction between the particles increases much more strongly than electric double layer repulsion from the adsorption of ammonium dibutyl dithiophosphate. It is also found that the floc flotation is closely correlated with the size of flocs,which is strongly influenced by the stirring strength and enhanced by the addition of a small amount of kerosene. The results of FTIR spectra indicate that adsorption of ammonium dibutyl dithiophosphate onto jamesonite is chemical adsorption and the adsorption product is lead dibutyl dithiophosphate.
基金grants from the Zhejiang Provincial Key R&D Program(2019C03110)the National Natural Science Foundation of China(Grant No.51578538).
文摘In recent years,the presence and adverse impacts of phthalic acid esters m aquatic environments have gained increasing attention.This work investigated the adsorption behavior of a typical phthalic acid ester,dibutyl phthalate(DBP),onto steel slag,gravel,and shell sand(substrates commonly used in constructed wetlands).The influence of dissolved organic matter(DOM)on DBP adsorption was investigated using humic acid as a proxy for DOM.The results demonstrated that the adsorption of DBP to three substrates reached equilibrium within 96 h,and the adsorption kinetics were well fitted by a pseudo-second-order model.The DBP adsorption isotherms were best fitted by the Langmuir adsorption model.The DBP adsorption capacity decreased in the order of steel slag>gravel>sheII sand,with values of 656 mg/kg,598 mg/kg,and 6.62 mg/kg at 25°C,respectively.DBP adsorbed to the surface of all substrates in a monolayer via an endothermic process.The DBP adsorption capacities of steel slag and gravel decreased as the DOM content increased.The DBP adsorption mechanisms to steel slag and gravel mainly involved the surface coordination of DBP with-OH or-COOH groups and electrostatic interactions.The results of this work suggest that steel slag and gravel may be ideal substrates for use in constructed wetlands to treat wastewater polluted with DBP.
