In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids....In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids.Initially,potential endophytic fungi were identified through genome mining.Subsequently,fine fractions containing various fernane-type triterpenoids were selected using HSQC data collection and SMART prediction.These triterpenoids were then obtained through targeted isolation and identification.Finally,their antifungal activity was evaluated.As a result,three fernane-type triterpenoids,including two novel compounds,along with two new sesquiterpenes and four known compounds were isolated from one potential strain,Diaporthe discoidispora.Their structures were elucidated through analysis of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(NMR)spectroscopic data.The absolute configurations were determined using single-crystal X-ray diffraction analysis and electron capture detector(ECD)analysis.Compound 3 exhibited moderate antifungal activity against Candida albicans CMCC 98001 and Aspergillus niger.展开更多
This study identified six novel azaphilones,isochromophilones G-L(1-6),and three novel biosynthetically related congeners(7-9)from Diaporthe sp.SCSIO 41011.The structures and absolute configurations were elucidated th...This study identified six novel azaphilones,isochromophilones G-L(1-6),and three novel biosynthetically related congeners(7-9)from Diaporthe sp.SCSIO 41011.The structures and absolute configurations were elucidated through comprehensive spectroscopic analyses combined with experimental and calculated electronic circular dichroism(ECD)spectra.Significantly,three highly oxygenated azaphilones contain an acetyl group at the terminal chain(4)or linear conjugated polyenoid moieties(5 and 6),which occur infrequently in the azaphilone family.Additionally,several compounds demonstrated inhibition of lipopolysaccharide(LPS)-induced nuclear factor kappa-B(NF-κB)activation in RAW 264.7 macrophages at 20μmol·L^(-1).The novel compound(1)effectively inhibited receptor activator of NF-κB ligand(RANKL)-induced osteoclast differentiation without exhibiting cytotoxicity in bone marrow and RAW264.7 macrophages,indicating its potential as a promising lead compound for osteolytic disease treatment.This research presents the first documented evidence of azaphilone derivatives as inhibitors of RANKL-induced osteoclastogenesis.展开更多
A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots...A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.展开更多
Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures ...Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures were elucidated by extensive HRESIMS and NMR spectroscopic analyses.All compounds were tested for their cytotoxicities against five human cancer cell lines by MTT method,among which compound 1 showed moderate inhibitory effects on these cell lines.This was the first report of azaphilones isolated from Diaporthe sp.展开更多
t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on...t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on Persea americana and D.salicicola on Salix purpurea from Australia are described and illustrated based on morphological characteristics and molecular analyses.Three of the new species no longer produced sporulating structures in culture and two of these were morphologically described from voucher specimens.Phylogenetic relationships of the new species with other Diaporthe species are revealed by DNA sequence analyses based on the internal transcribed spacer(ITS)region,and partial regions of theβtubulin(BT)and translation elongation factor 1-alpha(TEF).展开更多
Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of...Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.展开更多
基金supported by the Outstanding Youth Foundation of Heilongjiang Province(No.YQ2021H009).
文摘In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids.Initially,potential endophytic fungi were identified through genome mining.Subsequently,fine fractions containing various fernane-type triterpenoids were selected using HSQC data collection and SMART prediction.These triterpenoids were then obtained through targeted isolation and identification.Finally,their antifungal activity was evaluated.As a result,three fernane-type triterpenoids,including two novel compounds,along with two new sesquiterpenes and four known compounds were isolated from one potential strain,Diaporthe discoidispora.Their structures were elucidated through analysis of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(NMR)spectroscopic data.The absolute configurations were determined using single-crystal X-ray diffraction analysis and electron capture detector(ECD)analysis.Compound 3 exhibited moderate antifungal activity against Candida albicans CMCC 98001 and Aspergillus niger.
基金supported by the National Natural Science Foundation of China(No.82260692)Guangxi Natural Science Foundation(No.2024GXNSFFA010004)+2 种基金Bagui Youth Talent Development ProgramHigh-Level Talent Training Project Foundation of Guangxi University of Chinese Medicine(Nos.202407 and 2022C038)the Innovation Project of Guangxi Graduate Education(No.YCSW2025457)。
文摘This study identified six novel azaphilones,isochromophilones G-L(1-6),and three novel biosynthetically related congeners(7-9)from Diaporthe sp.SCSIO 41011.The structures and absolute configurations were elucidated through comprehensive spectroscopic analyses combined with experimental and calculated electronic circular dichroism(ECD)spectra.Significantly,three highly oxygenated azaphilones contain an acetyl group at the terminal chain(4)or linear conjugated polyenoid moieties(5 and 6),which occur infrequently in the azaphilone family.Additionally,several compounds demonstrated inhibition of lipopolysaccharide(LPS)-induced nuclear factor kappa-B(NF-κB)activation in RAW 264.7 macrophages at 20μmol·L^(-1).The novel compound(1)effectively inhibited receptor activator of NF-κB ligand(RANKL)-induced osteoclast differentiation without exhibiting cytotoxicity in bone marrow and RAW264.7 macrophages,indicating its potential as a promising lead compound for osteolytic disease treatment.This research presents the first documented evidence of azaphilone derivatives as inhibitors of RANKL-induced osteoclastogenesis.
基金Supported by Science and Technology Project of Guangdong Province in China(2017A020213003)the Opening Project Fund of Key Laboratory of Guangdong Institute of Traditional Chinese Medicine(GDTCMZJ201901)。
文摘A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae.
基金This work was co-financed by National Natural Science Foundation of China(81121062,21132004,90813036,and 81172948),MOE(NCET-10-0477)Jiangsu Provincial Government(BK2009010).
文摘Three new azaphilone compounds,isochromophilones X-XII(1-3),together with two known ones sclerotioramine(4)and isochromophilone VI(5)were isolated from the cultures of an endophytic fungus Diaporthe sp.The structures were elucidated by extensive HRESIMS and NMR spectroscopic analyses.All compounds were tested for their cytotoxicities against five human cancer cell lines by MTT method,among which compound 1 showed moderate inhibitory effects on these cell lines.This was the first report of azaphilones isolated from Diaporthe sp.
文摘t Six new species of Diaporthe,D.beilharziae on Indigofera australis,D.fraxini-angustifoliae on Fraxinus angustifolia subsp.oxycarpa,D.litchicola on Litchi chinensis,D.nothofagi on Nothofagus cunninghamii,D.pascoei on Persea americana and D.salicicola on Salix purpurea from Australia are described and illustrated based on morphological characteristics and molecular analyses.Three of the new species no longer produced sporulating structures in culture and two of these were morphologically described from voucher specimens.Phylogenetic relationships of the new species with other Diaporthe species are revealed by DNA sequence analyses based on the internal transcribed spacer(ITS)region,and partial regions of theβtubulin(BT)and translation elongation factor 1-alpha(TEF).
基金supported by China Agriculture Research System(CARS-27)Research Programs for Nonprofit Technology and Application of Zhejiang Province,China(2009CB119203)。
文摘Diaporthe species are important pathogens of a wide range of hosts including many economically important crops.Diaporthe citri is generally accepted to cause melanose of Citrus fruits,leaves and shoots;stem-end rot of fruits and gummosis of perennial branches.In this study,147 strains of Diaporthe were isolated from diseased leaves,twigs,fruits and branches of Citrus from Shaanxi,Zhejiang,Guangxi,Fujian,Guangdong,Jiangxi,Hunan,Chongqing and Shanghai and identified by morphology and molecular analysis of nuclear ribosomal internal transcribed spacer(ITS),partial sequences of translation elongation factor 1-α(EF 1-α),tubulin(TUB)and calmodulin(CAL)genes.All strains from Citrus clustered in three clades.Diaporthe citri was the predominant species and is compared with strains from Florida,USA,where this species was first collected and described.Two new taxa differing from D.citri are described as D.citriasiana and D.citrichinensis in this paper.Inoculation tests indicate that all three species are able to cause stem-end rot of fruits,while only D.citri could cause melanose on leaves.
