The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been syste...The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been systematically studied.The author applied Chaihu Wendan decoction to treat phlegm and qi stagnation in the middle energizer,applied Chaihu Guizhi decoction to treat simultaneous disease of Shaoyang and Taiyang,applied Chaihu Xiexin decoction to treat simultaneous disease of the spleen,stomach,liver,and gallbladder,applied Chaihu Ermiao powder to treat simultaneous disease of hand Shaoyang and foot Taiyin,applied Chaihu Erchen decoction to treat simultaneous disease of Shaoyang and Taiyin,applied Chailing decoction to treat simultaneous disease of hand Shaoyin and foot Taiyang,applied Chaihu Guizhi Ganjiang decoction to treat Shaoyang and Taiyin cold fluid retention and heat stagnation syndrome of Shaoyang complicated with Taiyin,applied Chaihu Xingren decoction to treat simultaneous disease of hand Shaoyang and hand Taiyin,applied Chaihu Sanren decoction to treat the disease caused by damp-heat stagnation in the hand Shaoyang and the foot Taiyin,and applied Chaihu Ganlu Xiaodu pellet to treat the diseases of hand Shaoyang and foot Yangming.The effects were all remarkable.展开更多
Background:ZhiZi-BoPi Decoction(ZZBPD),a traditional prescription for liver and gallbladder protection,has garnered significant clinical interest due to its hepatoprotective properties.Despite its proven efficacy in m...Background:ZhiZi-BoPi Decoction(ZZBPD),a traditional prescription for liver and gallbladder protection,has garnered significant clinical interest due to its hepatoprotective properties.Despite its proven efficacy in mitigating intrahepatic cholestasis,the precise mechanisms underlying its therapeutic effects remain inadequately understood.This study aims to comprehensively investigate the pharmacological mechanisms underlying the therapeutic effects of ZZBPD in cholestatic liver injury(CLI).Methods:Firstly,we evaluated the hepatoprotective effects of ZZBPD on mice with CLI induced byα-naphthylisothiocyanate(ANIT),by measuring biochemical markers,inflammatory factors,and bile acid levels.Subsequently,we employed network pharmacology and single-cell RNA sequencing(scRNA-seq)to identify key targets and potential signaling pathways for the prevention and treatment of CLI.Finally,we further validated the mechanism of action of ZZBPD on these key targets through molecular docking,western blotting,and immunofluorescence techniques.Results:ZZBPD notably improved serum liver function,reduced hepatic inflammation,and restored bile acid balance.Through network pharmacology and scRNA-seq analysis,48 core targets were identified,including TNF,IL-6,and NFKB1,all of which are linked to the IL-17 and NF-κB signaling pathways,as shown by KEGG enrichment analysis.Molecular docking further confirmed stable interactions between ZZBPD’s key active components and molecules such as IL-6,IL-17,and NF-κB.Additionally,western blotting and immunofluorescence validated the downregulation of IL-17 and NF-κB protein expression in liver tissue.Conclusion:ZZBPD effectively treats CLI by activating pathways related to the bile acid receptor FXR,while also modulating the IL-17/NF-κB signaling pathway.This dual action enhances bile secretion and alleviates liver inflammation.These findings offer important insights into the pharmacological mechanisms of ZZBPD and underscore its potential as a promising therapeutic for CLI.展开更多
OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and N...OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and Nuangong Tiaojing decoction(暖宫调经方,NGTJ-D).METHODS:This study was undertaken in the Jiangsu Province Hospital of Chinese Medicine.Altogether 90 eligible patients with PCOS and LPD were assigned to exposed group A(Chinese Medicine therapy,YXDJ-D and NGTJ-D),exposed group B(Chinese Medicine plus Western Medicine therapy),control group(Western Medicine therapy).The exposed group A adopted the sequential therapy that YXDJ-D is taken in the postmenstrual period(follicular phase)and NGTJ-D is taken in premenstrual period(luteal phase).Control group took letrozole,dydrogesterone and was given intramuscular injection of human menopausal gonadotropin,human chorionic gonadotropin.The exposed group B was treated with the above-mentioned therapy project of integrated Chinese Medicine and Western Medicine.This study lasted for 2 courses for 6 months.The primary outcomes were pregnancy rate and early abortion rate.The secondary outcomes were the Traditional Chinese Medicine(TCM)syndrome scores,estrogen(E2)and progesterone(P),endometrial volume(EV),vascularity index(VI),flow index(FI)and vascularization flow index(VFI).These outcomes will be assessed at baseline and post-intervention.RESULTS:The pregnancy rates of the exposed group A and B were higher than the control group(60.00%vs 60.00%vs 53.33%),while early abortion rates of exposed groups A and B were lower than the control group(33.33%vs 16.67%vs 43.75%,P>0.05).Total efficacy rates in exposed group A and B were better than the control group(93.30%vs 93.30%vs 53.30%,P<0.01).TCM symptom scores and endometrial receptivity indexes(EV,FI,VFI)were significantly lower in exposed groups compared to the control group(P<0.05).P increase in exposed group B was superior to the other two groups(P<0.01).No noticeable abnormalities in safety indicators in the three groups.CONCLUSION:The sequential therapy of YXDJ-D and NGTJ-D can effectively increase pregnancy rate,reduce the early abortion rate and alleviate the clinical symptoms of infertility in patients with PCOS and LPD by improving luteal function and promoting the endometrial receptivity.展开更多
Objective:To explore the improvement effect of implementing Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction on cardiac function and quality of life of patients during the treatment of coronary heart disease...Objective:To explore the improvement effect of implementing Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction on cardiac function and quality of life of patients during the treatment of coronary heart disease complicated with heart failure.Methods:Eighty cases were included in the study,and they were equally divided into a control group(n=40,treated with basic western medicine)and a study group(n=40,treated with Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction)according to random sampling grouping method.The intervention index results of the two groups were compared.Results:The improvement of cardiac function index,TCM syndrome score,and quality of life in the study group was more prominent,with a statistical value of P<0.05.Conclusion:Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction and conventional western medicine treatment can effectively improve the cardiac function of patients with coronary heart disease complicated with heart failure and enhance their quality of life.It is worthy of clinical promotion and application.展开更多
Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has ...Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has been proved that traditional Chinese medicine(TCM)has displayed definite therapeutic effects on viral hemorrhagic fever,indicating its potential to treat SFTS.In this study,SFTS-relative key targets were predicted via gene ontology(GO)analysis and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Molecular docking was then used to select stable binders.Molecules matched TCMs were identified,and a new prescription,Qingqi Guxue decoction(QQGX),was formulated to clear heat and nourish blood,with a resulting drug composition network.We explored the optimal drug proportion for QQGX.Through an in-depth study of molecular mechanisms,we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2(CCNA2)and cyclin-dependent kinase 2(CDK2),thereby inhibiting SFTSV replication.Finally,we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV.In summary,our study prepared a TCM decoction using the method of network pharmacology.This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.展开更多
Objective This study aimed to study the effects of different crystalline states of Sheng Shigao(raw gypsum,RG)and its inorganic elements on the antipyretic efficacy of Baihu Decoction(BHT).Methods RG samples calcined ...Objective This study aimed to study the effects of different crystalline states of Sheng Shigao(raw gypsum,RG)and its inorganic elements on the antipyretic efficacy of Baihu Decoction(BHT).Methods RG samples calcined at different temperatures were prepared.The phase composition of RG and Duan Shigao(calcination of gypsum,CG)as well as the changes in phase composition before and after adding water to RG calcined at specific temperatures,were determined using X-ray diffraction(XRD).A fever model was established by subcutaneously injecting 20%yeast suspension(10 mL·kg~(-1))into the backs of rats.The effects of BHT containing RG in different crystalline states on rat body temperature were measured.Serum levels of IL-1β,IL-6,and hypothalamic prostaglandin E2(PGE_2)were detected using ELISA.Serum Ca~(2+)levels were measured using a microplate method.The content of trace elements in RG and CG and the corresponding freeze-dried BHT powder was determined using inductively coupled plasma mass spectrometry(ICP-MS).The complexation of representative inorganic elements with mangiferin,a major active component in BHT,was investigated using UV-Vis spectroscopy and fluorescence spectroscopy.A validation model was established using RAW264.7 mouse macrophages.Drug-containing serum of BHT with different inorganic elements was prepared,and the nitric oxide(NO)levels in the cell supernatant of different treatment groups were measured using the Griess method.The mRNA levels of IL-6,TNF-α,and PGE2in each group were detected using qPCR(real-time fluorescent quantitative PCR).Results After calcination,the phase composition of RG changed,and the content of inorganic elements in RG,CG170(RG calcined at 170°C),and CG350(RG calcined at 350°C)showed similar trends.Compared with RG,the content of Ca,Sr,Al,and Na in CG changed significantly.Compared with BHT,the content of Ca,Sr,Si,and Na in CG changed significantly when incorporated into the formula.Intermolecular interactions confirmed strong binding between mangiferin and Cu~(2+)and Al~(3+).Cu~(2+)and Fe~(3+)exhibited fluorescence quenching effects on mangiferin solution,while Al~(3+)and Zn~(2+)showed strong fluorescence enhancement,with fluorescence intensity increasing by 120-fold and 30-fold,respectively.In vitro evaluation of synergistic anti-inflammatory effects confirmed that Ca,Fe,Cr,Al,and Si exhibited synergistic anti-inflammatory effects.Conclusion The crystalline state of RG has little effect on its antipyretic properties,while Ca,Sr,Na,Fe,and Al are likely the key material bases influencing its efficacy.展开更多
BACKGROUND The development of slow transit constipation(STC)is associated with intestinal barrier damage.Huangqi decoction(HQD)is effective in treating STC,but me-chanisms are unclear.AIM To investigate whether HQD al...BACKGROUND The development of slow transit constipation(STC)is associated with intestinal barrier damage.Huangqi decoction(HQD)is effective in treating STC,but me-chanisms are unclear.AIM To investigate whether HQD alleviates STC by downregulating the nuclear factorκB(NF-κB)signaling pathway and restoring intestinal barrier function.METHODS KM mice were divided into control,model,and HQD treatment groups.Fresh colonic tissues were collected for single-cell RNA sequencing and spatial tra-nscriptome sequencing.The expressions of claudin-1,mucin 2,and NF-κB P65 proteins were detected by immunohistochemistry.In vitro experiments evaluated the effects of HQD on the LS174T cell line.RESULTS HQD improved intestinal motility,restored mucosal epithelium function and morphology.Single-cell RNA sequencing and spatial transcriptome sequencing data showed a reduction in goblet cells,decreased mucin 2 secretion,and activated apoptotic pathways in STC mice.The population of intestinal stem cells was reduced,and proliferation along with Wnt/β-catenin pathways were inhibited.STC also altered the distribution of intestinal cell states,increasing immune-associated Enterocyte_C3.Aberrant NF-κB pathway activation was noted across various cell types.After HQD treatment,NF-κB pathway activity was down-regulated,while cell proliferation pathways were up-regulated,alongside an increase in Enterocyte_C1 related to material transport.Immunocytochemical,Western blot,and immunohistochemistry analyses confirmed NF-κB pathway activation in goblet cells of STC mice,with HQD inhibiting this aberrant activation.CONCLUSION STC involves intestinal mucosal barrier damage.HQD may treat STC by suppressing NF-κB signaling in epithelial cells,restoring intestinal epithelial cell function,and promoting mucosal barrier repair.展开更多
Traditional Chinese medicine has unique advantages in preventing and treating COVID-19,and Fuzheng Jiedu decoction(FZJDD)was reported to be effective against COVID-19 in clinical trials.To investigate the potential me...Traditional Chinese medicine has unique advantages in preventing and treating COVID-19,and Fuzheng Jiedu decoction(FZJDD)was reported to be effective against COVID-19 in clinical trials.To investigate the potential mechanisms and material basis of FZJDD against SARS-CoV-2,we performed SARS-CoV-2 target protein inhibition analyses and a metabolite full spectrum analysis of FZJDD.Interestingly,FZJDD was found to block the binding of SARS-CoV-2 Spike protein with the receptor ACE2 and inhibit the activity of SARS-CoV-23CLpro.Moreover,FZJDD can regulate the TNF and the MAPK signaling pathway to inhibit the inflammatory response and alleviate the“cytokine storm”.A total of 298 compounds were identified in FZJDD,among them,caffeic acid and octyl gallate were found to be the potential therapeutic agents of FZJDD.Importantly,FZJDD can broadly inhibit coronavirus infection,including SADS-CoV and porcine epidemic diarrhea virus(PEDV)live viruses,SARS-CoV,MERS-CoV,and SARS-CoV-2 mutant pseudotyped viruses,which might be ascribed to the broad-spectrum anti-coronavirus activity of caffeic acid and octyl gallate.In conclusion,this study reveals the mechanisms and material basis of FZJDD against SARS-CoV-2 and identifies the broadspectrum anti-coronavirus activity of FZJDD for the first time.Our data provide empirical evidence for the development and application of FZJDD.展开更多
OBJECTIVE:To evaluate the effect of Fuzi Lizhong decoction(附子理中汤)on intestinal flora,serum inflammatory factors,and hypoxia inducible factor-1α(HIF-1α)in patients with colorectal cancer associated with spleen a...OBJECTIVE:To evaluate the effect of Fuzi Lizhong decoction(附子理中汤)on intestinal flora,serum inflammatory factors,and hypoxia inducible factor-1α(HIF-1α)in patients with colorectal cancer associated with spleen and kidney Yang deficiency.METHODS:A total of 100 patients diagnosed with advanced colorectal cancer were randomly divided into two groups:a control group(CON,50)and a Traditional Chinese Medicine(TCM)group(n=50).The control group received treatment with the Capecitabine+Oxaliplatin(CAPEOX)regimen,while the TCM group received the same regimen along with Fuzi Lizhong decoction for six weeks.Changes in intestinal flora were assessed before and after six weeks in both groups.Serum markers,including HIF-1α,vascular endothelial growth factor(VEGF),interleukin-6(IL-6),and tumor necrosis factor-alpha(TNF-α),were measured using enzyme-linked immunosorbent assay.Adverse reactions,clinical efficacy,and TCM syndrome efficacy were also monitored.RESULTS:After six weeks,the levels of Lactobacillus and Bifidobacterium were significantly higher,while the levels of Enterobacter and Enterococcus were significantly lower in the TCM group compared to the control group(P<0.05).Serum levels of HIF-1α,VEGF,IL-6,and TNF-αwere also significantly reduced in the TCM group compared to the control group(P<0.05).Additionally,the incidence of adverse reactions was lower,and the clinical efficacy was higher in the TCM group compared to the control group(P<0.05).CONCLUSION:Fuzi Lizhong decoction effectively improves intestinal microbiota composition,reduces inflammatory factors and HIF-1αexpression,alleviates chemotherapy-related adverse reactions,enhances clinical efficacy,and may inhibit tumor growth in patients with colorectal cancer.展开更多
Lingguizhugan Decoction(LGZG)demonstrates significant efficacy in treating various cardiovascular diseases clinically,yet its precise mechanism of action remains elusive.This study aimed to elucidate the potential mec...Lingguizhugan Decoction(LGZG)demonstrates significant efficacy in treating various cardiovascular diseases clinically,yet its precise mechanism of action remains elusive.This study aimed to elucidate the potential mechanisms and effects of LGZG on isoproterenol(ISO)continuous stimulation-induced chronic heart failure(CHF)in mice,providing direct experimental evidence for further clinical applications.In vivo,continuous ISO infusion was administered to mice,and ventricular myocytes were utilized to explore LGZG's potential mechanism of action on theβ1-adrenergic receptor(β1-AR)/Gs/G protein-coupled receptor kinases(GRKs)/β-arrestin signaling deflection system in the heart.The findings reveal that LGZG significantly reduced the messenger ribonucleic acid(mRNA)expression of hypertrophy-related biomarkers[atrial natriuretic peptide(ANP)and B-type natriuretic peptide(BNP)]and improved cardiac remodeling and left ventricular diastolic function in mice with ISO-induced CHF.Furthermore,LGZG inhibited the overactivation of Gs/cyclic adenosine monophosphate(c AMP)/protein kinase A(PKA)signaling and downregulated the downstream transcriptional activity of c AMP-response element binding protein(CREB)and the expression of the coactivator CBP/P300.Notably,LGZG downregulated the expression ofβ-arrestin1 and GRK 2/3/5 while upregulating the expression ofβ1-AR andβ-arrestin2.These results suggest that LGZG inhibits Gs/c AMP/PKA signaling andβ-arrestin/GRK-mediated desensitization and internalization ofβ1-AR,potentially exerting cardioprotective effects through the synergistic regulation of theβ1-AR/Gs/GRKs/β-arrestin signaling deflection system via multiple pathways.展开更多
Objective This study aimed to investigate the therapeutic effects and underlying mechanisms of the combination of Yinchenhao decoction(YCHD)and praziquantel(PZQ)in a Schistosoma japonicum(S.japonicum)-induced mouse mo...Objective This study aimed to investigate the therapeutic effects and underlying mechanisms of the combination of Yinchenhao decoction(YCHD)and praziquantel(PZQ)in a Schistosoma japonicum(S.japonicum)-induced mouse model of schistosomiasis.Methods Six-week-old male BALB/c mice were randomly divided into five groups:control group,infected group,infected-PZQ group(I-PZQ),infected-YCHD group(I-YCHD),and infected-PZQ+YCHD group(I-PZQ+YCHD).The mice were infected with S.japonicum cercariae in infected group,I-PZQ group,I-YCHD group,and I-PZQ+YCHD group(n=6 per group)and maintained for 63 days.From day 43 to day 63 postinfection,the mice received PZQ(150 mg/kg,intragastric gavage),YCHD(10 mL/kg,intragastric gavage),or a combination of both.The control and infected groups received equal amounts of sterile double-distilled water for the same period.At the end of the experiment,the mice were anesthetized with pentobarbital sodium and sacrificed.Serum alanine transaminase(ALT)and aspartate transaminase(AST)levels were measured.Network pharmacology analysis was used to predict the targets of YCHD in the treatment of schistosomiasis.Histopathological analysis,Western blotting,immunofluorescence,quantitative polymerase chain reaction and flow cytometry were employed to evaluate liver pathology and molecular changes.Results Compared with the other groups,the I-PZQ+YCHD group presented significantly decreased serum ALT and AST levels(P<0.001).The I-PZQ+YCHD group exhibited improved pathological changes in the liver,as evidenced by reduced area of single granuloma(P<0.01),granuloma area(P<0.01),and Ishak score of liver fibrosis(P<0.01).Network pharmacology analysis suggested that YCHD may alleviate schistosomiasis-related liver injury through the modulation of the endoplasmic reticulum stress(ERS)pathway.Western blot analysis revealed that ERS-related markers,including glucose-regulated protein 78(GRP78),inositol-requiring enzyme 1 alpha(IRE1α),X-box binding protein 1(XBP-1),and C/EBP homologous protein(CHOP),were significantly downregulated in the I-PZQ+YCHD group(P<0.05).Furthermore,the I-PZQ+YCHD group presented reduced hepatocyte apoptosis(P<0.05),diminished hepatic macrophage infiltration(P<0.05)and downregulated expression of proinflammatory cytokines(TNF-α,IL-1βand IL-6)(P<0.05).Conclusion YCHD combined with PZQ reduced schistosomiasis-associated hepatic granulomatous inflammation and fibrosis by inhibiting hepatic apoptosis and ERS.展开更多
OBJECTIVE:To investigate the mechanisms of the effect of Shenji Guben(SJGB)decoction(参吉固本方)on chronic obstructive pulmonary disease(COPD).METHODS:A murine model of COPD was established through lipopolysaccharide(...OBJECTIVE:To investigate the mechanisms of the effect of Shenji Guben(SJGB)decoction(参吉固本方)on chronic obstructive pulmonary disease(COPD).METHODS:A murine model of COPD was established through lipopolysaccharide(LPS)nasal drops and passive smoke exposure,followed by evaluation of SJGB decoction efficacy via lung function tests and histological analysis.Non-targeted liquid chromatography-mass spectrometry(LC-MS)-based metabolomics was used to explore the mechanisms of SJGB decoction in COPD.RESULTS:We found that the SJGB decoction effectively reduced inflammatory cell infiltration in the airways and lungs,and improved lung function in the COPD model mice.LC-MS-based metabolomics identified 86 biomarkers in COPD models.Compared to the model group,SJGB decoction significantly altered 34 metabolites.Prostaglandin E2 and DL-Citrulline were highlighted as two representative differential metabolites.MetaboAnalyst 5.0 highlighted glycerophospholipid and riboflavin metabolisms as key pathways affected by SJGB decoction.CONCLUSION:This study evaluated the protective effect of SJGB decoction against COPD and provided insights into its potential mechanisms in COPD treatment.展开更多
Background:Diabetic kidney disease(DKD)is a major cause of end-stage renal disease,with limited effective treatment options currently available.Shenqi Dihuang Decoction(SQDH)has demonstrated clinical efficacy in manag...Background:Diabetic kidney disease(DKD)is a major cause of end-stage renal disease,with limited effective treatment options currently available.Shenqi Dihuang Decoction(SQDH)has demonstrated clinical efficacy in managing DKD;however,the metabolic mechanisms responsible for its therapeutic effects remain unclear.Methods:We established a DKD mouse model and treated the mice with SQDH to investigate its effects on renal function and tissue pathology.To explore the metabolic mechanisms,we conducted non-targeted metabolomics to identify differential metabolites in the renal tissues of DKD mice and the associated metabolic pathways affected by SQDH.Additionally,we performed RT-qPCR and Western blot analyses to assess the effects of SQDH on the expression of key genes and proteins within the targeted pathways.To further evaluate SQDH’s therapeutic effects,we measured oxidative stress markers and inflammatory factors,examining its antioxidant and anti-inflammatory properties in DKD.Results:SQDH treatment improved body weight and blood glucose levels in DKD mice.It also restored renal function,as indicated by improved 24h-UTP,serum creatinine,and blood urea nitrogen levels,and alleviated renal tissue pathology associated with DKD.Metabolomic analysis showed that SQDH primarily regulates glycerophospholipid metabolism,particularly by increasing phosphatidylcholine(PC)levels and decreasing lysophosphatidylcholine(LPC)levels.RT-qPCR and Western blot analyses revealed that SQDH upregulated LPCAT expression and downregulated PLA2G expression.Additionally,SQDH enhanced the activities of superoxide dismutase and glutathione peroxidase,reduced reactive oxygen species,4-hydroxy-2-nonenal,and malondialdehyde levels,and decreased the levels of inflammatory cytokines IL-1β,IL-6,and TNF-α.Conclusion:Our findings confirm that SQDH protects against DKD by regulating glycerophospholipid metabolism,restoring the balance of PC and LPC,inhibiting inflammatory responses,and reducing oxidative stress.展开更多
Objectives:Irinotecan(CPT-11)-induced delayed diarrhea is a major dose-limiting toxicity that restricts its clinical application.Gegen Qinlian decoction(GQD),a traditional Chinese herbal formula,has shown potential in...Objectives:Irinotecan(CPT-11)-induced delayed diarrhea is a major dose-limiting toxicity that restricts its clinical application.Gegen Qinlian decoction(GQD),a traditional Chinese herbal formula,has shown potential in alleviating CPT-11-induced intestinal injury,but its underlying mechanisms remain incompletely understood.This study aimed to systematically elucidate the protective effects and mechanisms of GQD against CPT-11 enterotoxicity.Methods:We integrated ultra-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry(UPLC-QE-Orbitrap-MS)for chemical profiling of GQD,along with network pharmacology,molecular docking,and molecular dynamics simulation to identify key bioactive constituents and potential targets.Furthermore,a CPT-11-treated mouse model and Caco-2 cell monolayers were employed to evaluate the effects of GQD.Quantitative proteomics and untargeted metabolomics were applied to explore pathway alterations,and biochemical,histological,and functional analyses were conducted to assess inflammatory responses,oxidative stress,barrier integrity,and drug transporter activity.Results:A total of 65 compounds were identified in GQD,with 17 prioritized as putative bioactive constituents.Network analysis highlighted the IL-6/IL-22/STAT3 inflammatory axis and ABC transporter pathways as central mechanisms.In vivo,GQD administration ameliorated diarrhea,preserved colonic architecture and mucin production,reduced pro-inflammatory cytokines and oxidative stress,and restored expression of tight-junction proteins.Multi-omics integration showed that GQD counteracted CPT-11-induced dysregulation in complement/coagulation cascades and metabolic pathways,while enriching ABC transporter-related functions.In Caco-2 monolayers,GQD promoted SN-38 efflux,upregulated MDR1 and MRP2 expression,and decreased intracellular SN-38 accumulation.Consistent with this,GQD enhanced colonic clearance of SN-38 in mice.Conclusions:GQD protects against CPT-11-induced enterotoxicity primarily through two mechanisms:suppressing the pro-inflammatory IL-6/IL-22/STAT3 signaling pathway and enhancing the epithelial efflux of SN-38 via upregulation of ABC transporters.These findings provide a mechanistic basis for the potential use of GQD as an adjuvant therapy to mitigate intestinal toxicity during irinotecan chemotherapy.展开更多
BACKGROUND The deleterious effects of surgical trauma and subsequent postoperative complications pose significant challenges to the smooth recovery of patients after gastric cancer(GC)resection despite the substantial...BACKGROUND The deleterious effects of surgical trauma and subsequent postoperative complications pose significant challenges to the smooth recovery of patients after gastric cancer(GC)resection despite the substantial curative benefits provided by surgical interventions for GC.Hence,the investigation of more optimal and efficacious treatment approaches has become an urgent necessity in the medical community.AIM To investigate the association of Sijunzi decoction plus chemotherapy with the gastrointestinal function and serum markers of patients after GC surgery.METHODS This study included patients who underwent GC surgery from June 2022 to February 2024.The control group included 45 patients who received chemotherapy(oxaliplatin+calcium folinate+5-fluorouracil),whereas the research group consisted of 54 patients who received Sijunzi decoction therapy in addition to the treatment administered in the control group.Comparative analyses were conducted from the following perspectives:Gastrointestinal function(defecation time,intestinal gas discharge time,and hospitalization time),serum markers[carcinoembryonic antigen(CEA),carbohydrate antigen(CA)125,and CA199],nutritional indicators total protein(TP)and transferrin(TRF),traditional Chinese medicine(TCM)syndrome score,and grades Ⅲ–Ⅳ adverse events(gastrointestinal reactions,renal/liver function impairment,and myelosuppression).RESULTS The two groups demonstrated similar defecation time(P>0.05),but the intestinal gas discharge time and hospitalization time were significantly shortened in the research group(P<0.05).Further,the research group exhibited significant CEA,CA125,and CA199 reductions after treatment,which were lower compared to the control group,as well as notable increases in TP and TRF that were statistically higher than the control group(all P<0.05).Furthermore,the research group demonstrated an evident decrease in TCM syndrome scores in areas,such as poor appetite,epigastric distension and pain,fatigue and weakness(P<0.01),and abdominal distension after eating,which are notably lower than those in the control group(P<0.01),with a comparable incidence of grades Ⅲ-Ⅳ adverse events(P>0.05).CONCLUSION Our research results indicate that Sijunzi decoction plus chemotherapy exerts a good rehabilitation-promoting effect on gastrointestinal function in patients after GC surgery and significantly downregulates abnormally increased CEA,CA125,and CA199 levels.展开更多
Background:Damp-heat syndrome(DHS)is a complex condition in traditional Chinese medicine(TCM)that can cause various issues in circulation,digestion,and the respiratory system.This syndrome is believed to be closely li...Background:Damp-heat syndrome(DHS)is a complex condition in traditional Chinese medicine(TCM)that can cause various issues in circulation,digestion,and the respiratory system.This syndrome is believed to be closely linked to environmental factors such as high temperature and high humidity environments.Tingzhen Lu,a prominent physician during the Qing Dynasty,proposed the efficacy of Huanglian Wengdan Decoction(HLWDT)in addressing ailments stemming from high-temperature and high-humidity conditions.Nevertheless,the specific therapeutic effects of this decoction on DHS and its underlying mechanisms remain incompletely understood.Methods:To clarify the composition of HLWDT,mass spectrometry was utilized to identify the constituent compounds.Moreover,DHS rats induced by high humidity-temperature combined with a high sugar-fatty diet were treated with Huanglian Wendan decoction,the efficacy of which was evaluated based on serum biochemical indices and histopathological analyses.The expression of corresponding proteins was verified using western blotting.The mechanism of DHS relief by HLWDT was investigated by integration of network pharmacology and non-targeted metabolomics.Results:The HLWDT contained nearly 1,315 ingredients,the majority of which were flavonoids.Moreover,HLWDT not only regulated gastrointestinal motility and oxidative stress in DHS rats but also alleviated their inflammatory state.Metabolomics and network pharmacology analysis revealed that HLWDT primarily affects bile secretion,linoleic acid metabolism,PPAR,and MAPK signaling pathways.Furthermore,the PPAR signaling pathway was confirmed.HLWDT decreased the expression of NF-κB p65 and promoted MAPK phosphorylation and PPARγexpression in DHS rats.Conclusion:The therapeutic effect of HLWDT on DHS is potentially attributable to activation of the PPARγ-NF-κB/MAPK signaling pathway and regulation of oxidative stress and inflammatory responses.This experiment preliminarily elucidated the impact and mechanisms of HLWDT on DHS through pharmacodynamics,network pharmacology,and metabolomics.展开更多
OBJECTIVE:To explore the active compounds and the mechanism of Shenfu decoction(参附汤,SFD)against ischemic stroke(IS)through network pharmacology and animal experiments.METHODS:SFD components were retrieved from the ...OBJECTIVE:To explore the active compounds and the mechanism of Shenfu decoction(参附汤,SFD)against ischemic stroke(IS)through network pharmacology and animal experiments.METHODS:SFD components were retrieved from the Traditional Chinese Medicine(TCM)database.The Online Mendelian Inheritance in Man(OMIM),Comparative Toxicogenomics Database(CTD)and Therapeutic Target Database(TTD)database were used to retrieve the IS-related disease targets.The herb-compound-target network was built by Cytoscape 3.7.1 software.The core targets were obtained using protein-protein interaction(PPI)network.The core targets of SFD were further analyzed through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).We then performed molecular docking between the hub proteins and key active compounds.Finally,we conducted animal experiments to verify the regulation of SFD on apoptosis following IS.RESULTS:There were 221 corresponding targets and 25 components related to Chinese medicine throughout the compound-target network.The core targets of SFD in the treatment of IS was tumor protein P53(Tp53),mitogen-activated protein kinase 3(MAPK3),MAPK1,heat shock proteins 90AA1 and alpha serine/threonine-protein kinase1.There were 221 GO items in GO function enrichment analysis and 106 signaling pathways in KEGG,mainly including negative regulation of the apoptosis process,vascular endothelial growth factor signaling pathways,NOD-like receptor signaling pathway,etc.Among them,Tp53,MAPK3,and MAPK1 were docked with small molecule compounds.Through animal research,we confirmed the effect of SFD on apoptosis following stroke.CONCLUSION:This study demonstrates that SFD can treat IS through multiple targets and pathways,and provides new perspectives for exploring the core targets and mechanisms of SFD against IS.展开更多
Objective:To investigate the clinical efficacy of Xiaochaihu Decoction combined with Xiaoxianxiong Decoction in the treatment of post-stroke pneumonia.Methods:To complete the sample grouping comparison,all patients wi...Objective:To investigate the clinical efficacy of Xiaochaihu Decoction combined with Xiaoxianxiong Decoction in the treatment of post-stroke pneumonia.Methods:To complete the sample grouping comparison,all patients with post-stroke pneumonia were investigated,and the number of cases was 60.These patients’diseases were consistent with the dialectical standards of traditional Chinese medicine(phlegm-heat obstructing lungs).The patients were randomly divided into a control group(30 cases,treated with antibiotics and symptomatic methods)and a treatment group(30 cases,treated with Xiaochaihu Decoction and Xiaoxianxiong Decoction on the basis of the control group).Various indicators were compared.Results:The total clinical effective rates were 93%and 80%in the treatment group and the control group,respectively,with statistical significance(P<0.05).The improvement of various clinical symptoms was compared,and the values in the treatment group were reduced,showing significance(P<0.05).Analysis of serum factor indicators showed that the overall trend of the treatment group was reduced,and the comparison between groups was below 0.05.Conclusion:Xiaochaihu Decoction combined with Xiaoxianxiong Decoction has a significant clinical effect in the treatment of post-stroke pneumonia(phlegm-heat obstructing lungs syndrome),which can reduce inflammatory reactions and has few adverse reactions,worthy of clinical application.展开更多
BACKGROUND In China Banxia Xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years.BXD has been shown to reverse GC histopathology,but its chemical composition and action mechanism are ...BACKGROUND In China Banxia Xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years.BXD has been shown to reverse GC histopathology,but its chemical composition and action mechanism are still unknown.AIM To investigate the mechanism of BXD against GC based on utilizing transcrip-tomics and proteomics techniques experiments.METHODS Using the AGS cell line as the model group,the Cell Counting Kit-8 method and Annexin V-AbFluor™were employed 488/propidium iodide double staining method was used to detect the levels of cell proliferation and apoptosis.Differ-ential expression genes and differentially expressed proteins before and after BXD intervention were detected using RNA-seq and Pro DIA techniques.Key tran-scription factors were identified by enrichment and analysis using Metascape,and the key pathways were validated by western blot and reverse transcription PCR in vitro and in vivo experiments.RESULTS BXD significantly inhibited the proliferation rate and migration rate of GC cells and promoted cell apoptosis.The comprehensive analysis of transcriptomics and proteomics showed that five transcription factors,namely phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha,phosphoinositide-3-kinase regulatory subunit 1,AKT serine/threonine kinase 1,heat shock protein 90 alpha family class A member 1,and tumor protein p53,were key factors in BXD-mediated anti-cancer therapy and participated in the phosphatidylinositol 3-kinase(PI3K)/AKT signaling pathway.In vitro experiments were conducted using LY294002,an inhibitor of the PI3K/AKT signaling pathway,to validate the expression of five transcription factors at the protein and mRNA levels.In vivo experiments have shown that BXD inhibits tumor growth and suppresses the expression of the PI3K/AKT signaling pathway.CONCLUSION Transcriptomic and proteomic analysis showed that BXD inhibited tumor growth and slowed cancer progression by suppressing five factors in the PI3K/AKT signaling pathway,including phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha,phosphoinositide-3-kinase regulatory subunit 1,and AKT serine/threonine kinase 1.展开更多
Colorectal inflammatory cancer transformation poses a major risk to patients with colitis.Patients with chronic intestinal inflammation have an approximately 2–3 fold increased risk of developing colorectal cancer(CR...Colorectal inflammatory cancer transformation poses a major risk to patients with colitis.Patients with chronic intestinal inflammation have an approximately 2–3 fold increased risk of developing colorectal cancer(CRC).Unfortunately,there is currently no effective intervention available.Huangqin decoction(HQD),a well-known traditional Chinese medicine(TCM)formula,is frequently clinically prescribed for treating patients with colitis,and its active ingredients have effective antitumour efficacy.Nonetheless,the mechanism of HQD-mediated prevention of colorectal inflammatory cancer transformation remains unclear.A strategy integrating metagenomic,lipidomic,and messenger RNA(mRNA)sequencing analysis was used to investigate the regulatory effects of HQD on the gut microbiome,metabolism and potential mechanisms involved in colorectal inflammatory cancer transformation.Our study revealed that HQD suppressed colorectal inflammatory cancer transformation,which was associated with enhanced intestinal barrier function,decreased the inflammatory response,and regulation of the gut microbiome.Notably,cohousing experiments revealed that the transfer of the gut microbiome from HQD-treated mice largely inhibited the pathological transformation of colitis.Moreover,gut microbiome transfer from HQD-treated mice primarily resulted in the altered regulation of fatty acid metabolism,especially the remodeling of arachidonic acid metabolism,which was associated with the amelioration of pathological transformation.Arachidonic acid metabolism and the key metabolic enzyme arachidonic acid 12-lipoxygenase(ALOX12)were affected by HQD treatment,and no obvious protective effect of HQD was observed in Alox12−/−mice,which revealed that ALOX12 was a critical mediator of HQD protection against colorectal inflammatory cancer transformation.In summary,multiple omics analyses were applied to produce valuable data and theoretical support for the application of HQD as a promising intervention for the transformation of inflammatory CRC.展开更多
基金supported by Jiangxi Provincial Health Department Chinese Medicine Research Program Project{[2004]No.28}.
文摘The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been systematically studied.The author applied Chaihu Wendan decoction to treat phlegm and qi stagnation in the middle energizer,applied Chaihu Guizhi decoction to treat simultaneous disease of Shaoyang and Taiyang,applied Chaihu Xiexin decoction to treat simultaneous disease of the spleen,stomach,liver,and gallbladder,applied Chaihu Ermiao powder to treat simultaneous disease of hand Shaoyang and foot Taiyin,applied Chaihu Erchen decoction to treat simultaneous disease of Shaoyang and Taiyin,applied Chailing decoction to treat simultaneous disease of hand Shaoyin and foot Taiyang,applied Chaihu Guizhi Ganjiang decoction to treat Shaoyang and Taiyin cold fluid retention and heat stagnation syndrome of Shaoyang complicated with Taiyin,applied Chaihu Xingren decoction to treat simultaneous disease of hand Shaoyang and hand Taiyin,applied Chaihu Sanren decoction to treat the disease caused by damp-heat stagnation in the hand Shaoyang and the foot Taiyin,and applied Chaihu Ganlu Xiaodu pellet to treat the diseases of hand Shaoyang and foot Yangming.The effects were all remarkable.
基金supported by the National Science Foundation of China(No.82405004,82474253)the Natural Science Foundation postdoctoral project of Chongqing(CSTB2022NSCQ-BHX0709)+2 种基金Chongqing Wanzhou District doctoral“through train”scientific research project(wzstc-20220124)Natural Science Foundation of Chongqing,China(No.Cstc2021jcyj-msxmX0996)Chongqing Wanzhou District Science and Health Joint Medical Research Project(wzstc-kw2023032)。
文摘Background:ZhiZi-BoPi Decoction(ZZBPD),a traditional prescription for liver and gallbladder protection,has garnered significant clinical interest due to its hepatoprotective properties.Despite its proven efficacy in mitigating intrahepatic cholestasis,the precise mechanisms underlying its therapeutic effects remain inadequately understood.This study aims to comprehensively investigate the pharmacological mechanisms underlying the therapeutic effects of ZZBPD in cholestatic liver injury(CLI).Methods:Firstly,we evaluated the hepatoprotective effects of ZZBPD on mice with CLI induced byα-naphthylisothiocyanate(ANIT),by measuring biochemical markers,inflammatory factors,and bile acid levels.Subsequently,we employed network pharmacology and single-cell RNA sequencing(scRNA-seq)to identify key targets and potential signaling pathways for the prevention and treatment of CLI.Finally,we further validated the mechanism of action of ZZBPD on these key targets through molecular docking,western blotting,and immunofluorescence techniques.Results:ZZBPD notably improved serum liver function,reduced hepatic inflammation,and restored bile acid balance.Through network pharmacology and scRNA-seq analysis,48 core targets were identified,including TNF,IL-6,and NFKB1,all of which are linked to the IL-17 and NF-κB signaling pathways,as shown by KEGG enrichment analysis.Molecular docking further confirmed stable interactions between ZZBPD’s key active components and molecules such as IL-6,IL-17,and NF-κB.Additionally,western blotting and immunofluorescence validated the downregulation of IL-17 and NF-κB protein expression in liver tissue.Conclusion:ZZBPD effectively treats CLI by activating pathways related to the bile acid receptor FXR,while also modulating the IL-17/NF-κB signaling pathway.This dual action enhances bile secretion and alleviates liver inflammation.These findings offer important insights into the pharmacological mechanisms of ZZBPD and underscore its potential as a promising therapeutic for CLI.
基金Supported by National Natural Science Foundation of China:Elucidation of a New Mechanism of the Prescription for Reinforcing Kidney and Promoting Pregnancy (Bushen Zhuyun Decoction) in Treating Luteal Phase Defects Infertility based on Coenzyme Q10/Ubiquinol Mediated New Ferroptosis Pathway in Endometrium During Implantation (No. 82474567)Jiangsu Province Key Research and Development Plan (Society Development) Project:a Multicenter Real-world Study of Sequential Treatment of Luteal Phase Defects Infertility with “Improving Endometrial Receptivity” as the Effect Point (No. BE2021726)Project of Clinical Innovation Center of Traditional Chinese Medicine in Jiangsu Province:Clinical And Basic Research on the Treatment of Luteal Phase Defects Infertility with “Improving Endometrial Receptivity” as the Effect Point (No. k2021j18-1)
文摘OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and Nuangong Tiaojing decoction(暖宫调经方,NGTJ-D).METHODS:This study was undertaken in the Jiangsu Province Hospital of Chinese Medicine.Altogether 90 eligible patients with PCOS and LPD were assigned to exposed group A(Chinese Medicine therapy,YXDJ-D and NGTJ-D),exposed group B(Chinese Medicine plus Western Medicine therapy),control group(Western Medicine therapy).The exposed group A adopted the sequential therapy that YXDJ-D is taken in the postmenstrual period(follicular phase)and NGTJ-D is taken in premenstrual period(luteal phase).Control group took letrozole,dydrogesterone and was given intramuscular injection of human menopausal gonadotropin,human chorionic gonadotropin.The exposed group B was treated with the above-mentioned therapy project of integrated Chinese Medicine and Western Medicine.This study lasted for 2 courses for 6 months.The primary outcomes were pregnancy rate and early abortion rate.The secondary outcomes were the Traditional Chinese Medicine(TCM)syndrome scores,estrogen(E2)and progesterone(P),endometrial volume(EV),vascularity index(VI),flow index(FI)and vascularization flow index(VFI).These outcomes will be assessed at baseline and post-intervention.RESULTS:The pregnancy rates of the exposed group A and B were higher than the control group(60.00%vs 60.00%vs 53.33%),while early abortion rates of exposed groups A and B were lower than the control group(33.33%vs 16.67%vs 43.75%,P>0.05).Total efficacy rates in exposed group A and B were better than the control group(93.30%vs 93.30%vs 53.30%,P<0.01).TCM symptom scores and endometrial receptivity indexes(EV,FI,VFI)were significantly lower in exposed groups compared to the control group(P<0.05).P increase in exposed group B was superior to the other two groups(P<0.01).No noticeable abnormalities in safety indicators in the three groups.CONCLUSION:The sequential therapy of YXDJ-D and NGTJ-D can effectively increase pregnancy rate,reduce the early abortion rate and alleviate the clinical symptoms of infertility in patients with PCOS and LPD by improving luteal function and promoting the endometrial receptivity.
文摘Objective:To explore the improvement effect of implementing Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction on cardiac function and quality of life of patients during the treatment of coronary heart disease complicated with heart failure.Methods:Eighty cases were included in the study,and they were equally divided into a control group(n=40,treated with basic western medicine)and a study group(n=40,treated with Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction)according to random sampling grouping method.The intervention index results of the two groups were compared.Results:The improvement of cardiac function index,TCM syndrome score,and quality of life in the study group was more prominent,with a statistical value of P<0.05.Conclusion:Buzhong Yiqi Decoction combined with Xuefu Zhuyu Decoction and conventional western medicine treatment can effectively improve the cardiac function of patients with coronary heart disease complicated with heart failure and enhance their quality of life.It is worthy of clinical promotion and application.
基金supported by the National Natural Science Foundation of China(32170144 and 32470146).
文摘Severe fever with thrombocytopenia syndrome(SFTS)is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV),characterized by high fever and thrombocytopenia.It has been proved that traditional Chinese medicine(TCM)has displayed definite therapeutic effects on viral hemorrhagic fever,indicating its potential to treat SFTS.In this study,SFTS-relative key targets were predicted via gene ontology(GO)analysis and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.Molecular docking was then used to select stable binders.Molecules matched TCMs were identified,and a new prescription,Qingqi Guxue decoction(QQGX),was formulated to clear heat and nourish blood,with a resulting drug composition network.We explored the optimal drug proportion for QQGX.Through an in-depth study of molecular mechanisms,we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2(CCNA2)and cyclin-dependent kinase 2(CDK2),thereby inhibiting SFTSV replication.Finally,we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV.In summary,our study prepared a TCM decoction using the method of network pharmacology.This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.
基金Joint Fund Project of the Henan Provincial Science and Technology Research and Development Plan(222301420060)。
文摘Objective This study aimed to study the effects of different crystalline states of Sheng Shigao(raw gypsum,RG)and its inorganic elements on the antipyretic efficacy of Baihu Decoction(BHT).Methods RG samples calcined at different temperatures were prepared.The phase composition of RG and Duan Shigao(calcination of gypsum,CG)as well as the changes in phase composition before and after adding water to RG calcined at specific temperatures,were determined using X-ray diffraction(XRD).A fever model was established by subcutaneously injecting 20%yeast suspension(10 mL·kg~(-1))into the backs of rats.The effects of BHT containing RG in different crystalline states on rat body temperature were measured.Serum levels of IL-1β,IL-6,and hypothalamic prostaglandin E2(PGE_2)were detected using ELISA.Serum Ca~(2+)levels were measured using a microplate method.The content of trace elements in RG and CG and the corresponding freeze-dried BHT powder was determined using inductively coupled plasma mass spectrometry(ICP-MS).The complexation of representative inorganic elements with mangiferin,a major active component in BHT,was investigated using UV-Vis spectroscopy and fluorescence spectroscopy.A validation model was established using RAW264.7 mouse macrophages.Drug-containing serum of BHT with different inorganic elements was prepared,and the nitric oxide(NO)levels in the cell supernatant of different treatment groups were measured using the Griess method.The mRNA levels of IL-6,TNF-α,and PGE2in each group were detected using qPCR(real-time fluorescent quantitative PCR).Results After calcination,the phase composition of RG changed,and the content of inorganic elements in RG,CG170(RG calcined at 170°C),and CG350(RG calcined at 350°C)showed similar trends.Compared with RG,the content of Ca,Sr,Al,and Na in CG changed significantly.Compared with BHT,the content of Ca,Sr,Si,and Na in CG changed significantly when incorporated into the formula.Intermolecular interactions confirmed strong binding between mangiferin and Cu~(2+)and Al~(3+).Cu~(2+)and Fe~(3+)exhibited fluorescence quenching effects on mangiferin solution,while Al~(3+)and Zn~(2+)showed strong fluorescence enhancement,with fluorescence intensity increasing by 120-fold and 30-fold,respectively.In vitro evaluation of synergistic anti-inflammatory effects confirmed that Ca,Fe,Cr,Al,and Si exhibited synergistic anti-inflammatory effects.Conclusion The crystalline state of RG has little effect on its antipyretic properties,while Ca,Sr,Na,Fe,and Al are likely the key material bases influencing its efficacy.
基金Supported by the Natural Science Foundation of Guangdong Province for Distinguished Young Scholars,No.2022B1515020003the National Natural Science Foundation of China,No.82174369,No.82405397,No.82374442,and No.81973847+2 种基金Postdoctoral Fellowship Program of CPSF No.GZC20233247National Key Clinical Disciplineand the Program of Guangdong Provincial Clinical Research Center for Digestive Diseases,No.2020B1111170004.
文摘BACKGROUND The development of slow transit constipation(STC)is associated with intestinal barrier damage.Huangqi decoction(HQD)is effective in treating STC,but me-chanisms are unclear.AIM To investigate whether HQD alleviates STC by downregulating the nuclear factorκB(NF-κB)signaling pathway and restoring intestinal barrier function.METHODS KM mice were divided into control,model,and HQD treatment groups.Fresh colonic tissues were collected for single-cell RNA sequencing and spatial tra-nscriptome sequencing.The expressions of claudin-1,mucin 2,and NF-κB P65 proteins were detected by immunohistochemistry.In vitro experiments evaluated the effects of HQD on the LS174T cell line.RESULTS HQD improved intestinal motility,restored mucosal epithelium function and morphology.Single-cell RNA sequencing and spatial transcriptome sequencing data showed a reduction in goblet cells,decreased mucin 2 secretion,and activated apoptotic pathways in STC mice.The population of intestinal stem cells was reduced,and proliferation along with Wnt/β-catenin pathways were inhibited.STC also altered the distribution of intestinal cell states,increasing immune-associated Enterocyte_C3.Aberrant NF-κB pathway activation was noted across various cell types.After HQD treatment,NF-κB pathway activity was down-regulated,while cell proliferation pathways were up-regulated,alongside an increase in Enterocyte_C1 related to material transport.Immunocytochemical,Western blot,and immunohistochemistry analyses confirmed NF-κB pathway activation in goblet cells of STC mice,with HQD inhibiting this aberrant activation.CONCLUSION STC involves intestinal mucosal barrier damage.HQD may treat STC by suppressing NF-κB signaling in epithelial cells,restoring intestinal epithelial cell function,and promoting mucosal barrier repair.
基金supported by National Key Research and Development Program of China(grant No.2022YFC0867500,2020YFA0712102)National Natural Science Foundation of China(grant No.82151224,82202492)+3 种基金Fundamental Research Funds for Central Universities(grant No.QNTD 2023-01)Nutrition and Care of Maternal&Child Research Project of Biostime Institute of Nutrition&Care(grant No.2023BINCMCF28)State Key Laboratory of Pathogen and Biosecurity of China(grant No.SKLPBS2438)State Key Laboratory of Component-based Chinese Medicine(grant No.CBCM2024204).
文摘Traditional Chinese medicine has unique advantages in preventing and treating COVID-19,and Fuzheng Jiedu decoction(FZJDD)was reported to be effective against COVID-19 in clinical trials.To investigate the potential mechanisms and material basis of FZJDD against SARS-CoV-2,we performed SARS-CoV-2 target protein inhibition analyses and a metabolite full spectrum analysis of FZJDD.Interestingly,FZJDD was found to block the binding of SARS-CoV-2 Spike protein with the receptor ACE2 and inhibit the activity of SARS-CoV-23CLpro.Moreover,FZJDD can regulate the TNF and the MAPK signaling pathway to inhibit the inflammatory response and alleviate the“cytokine storm”.A total of 298 compounds were identified in FZJDD,among them,caffeic acid and octyl gallate were found to be the potential therapeutic agents of FZJDD.Importantly,FZJDD can broadly inhibit coronavirus infection,including SADS-CoV and porcine epidemic diarrhea virus(PEDV)live viruses,SARS-CoV,MERS-CoV,and SARS-CoV-2 mutant pseudotyped viruses,which might be ascribed to the broad-spectrum anti-coronavirus activity of caffeic acid and octyl gallate.In conclusion,this study reveals the mechanisms and material basis of FZJDD against SARS-CoV-2 and identifies the broadspectrum anti-coronavirus activity of FZJDD for the first time.Our data provide empirical evidence for the development and application of FZJDD.
基金Supported by the Guangxi Natural Science Foundation“Controllable Synthesis of Ordered Mesoporous Seafoam-Loaded g-C3N4 Gomposites and Their Mechanism of Adsorption-Photocatalytic Degradation of Antidepressants in Water Bodies”(2017GXNSFBA198216)the Open Fund for the Director of Guangxi Key Laboratory of Spatial Information and Geographic Information“Geographic Spatial Analysis of Regional Urinary Tract Stone Disease”(19-185-10-04)。
文摘OBJECTIVE:To evaluate the effect of Fuzi Lizhong decoction(附子理中汤)on intestinal flora,serum inflammatory factors,and hypoxia inducible factor-1α(HIF-1α)in patients with colorectal cancer associated with spleen and kidney Yang deficiency.METHODS:A total of 100 patients diagnosed with advanced colorectal cancer were randomly divided into two groups:a control group(CON,50)and a Traditional Chinese Medicine(TCM)group(n=50).The control group received treatment with the Capecitabine+Oxaliplatin(CAPEOX)regimen,while the TCM group received the same regimen along with Fuzi Lizhong decoction for six weeks.Changes in intestinal flora were assessed before and after six weeks in both groups.Serum markers,including HIF-1α,vascular endothelial growth factor(VEGF),interleukin-6(IL-6),and tumor necrosis factor-alpha(TNF-α),were measured using enzyme-linked immunosorbent assay.Adverse reactions,clinical efficacy,and TCM syndrome efficacy were also monitored.RESULTS:After six weeks,the levels of Lactobacillus and Bifidobacterium were significantly higher,while the levels of Enterobacter and Enterococcus were significantly lower in the TCM group compared to the control group(P<0.05).Serum levels of HIF-1α,VEGF,IL-6,and TNF-αwere also significantly reduced in the TCM group compared to the control group(P<0.05).Additionally,the incidence of adverse reactions was lower,and the clinical efficacy was higher in the TCM group compared to the control group(P<0.05).CONCLUSION:Fuzi Lizhong decoction effectively improves intestinal microbiota composition,reduces inflammatory factors and HIF-1αexpression,alleviates chemotherapy-related adverse reactions,enhances clinical efficacy,and may inhibit tumor growth in patients with colorectal cancer.
基金supported by the National Natural Science Foundation of China(No.82074054)Shanghai Municipal Commission of Health and Family Planning(No.ZY(2021-2023)-0208)+2 种基金Youth Program of the National Natural Science Foundation of China(No.81903831)Shanghai Municipality:Shanghai Chenguang Program(No.19CG48)Natural Science Foundation of Shanghai(No.24ZR1465900)。
文摘Lingguizhugan Decoction(LGZG)demonstrates significant efficacy in treating various cardiovascular diseases clinically,yet its precise mechanism of action remains elusive.This study aimed to elucidate the potential mechanisms and effects of LGZG on isoproterenol(ISO)continuous stimulation-induced chronic heart failure(CHF)in mice,providing direct experimental evidence for further clinical applications.In vivo,continuous ISO infusion was administered to mice,and ventricular myocytes were utilized to explore LGZG's potential mechanism of action on theβ1-adrenergic receptor(β1-AR)/Gs/G protein-coupled receptor kinases(GRKs)/β-arrestin signaling deflection system in the heart.The findings reveal that LGZG significantly reduced the messenger ribonucleic acid(mRNA)expression of hypertrophy-related biomarkers[atrial natriuretic peptide(ANP)and B-type natriuretic peptide(BNP)]and improved cardiac remodeling and left ventricular diastolic function in mice with ISO-induced CHF.Furthermore,LGZG inhibited the overactivation of Gs/cyclic adenosine monophosphate(c AMP)/protein kinase A(PKA)signaling and downregulated the downstream transcriptional activity of c AMP-response element binding protein(CREB)and the expression of the coactivator CBP/P300.Notably,LGZG downregulated the expression ofβ-arrestin1 and GRK 2/3/5 while upregulating the expression ofβ1-AR andβ-arrestin2.These results suggest that LGZG inhibits Gs/c AMP/PKA signaling andβ-arrestin/GRK-mediated desensitization and internalization ofβ1-AR,potentially exerting cardioprotective effects through the synergistic regulation of theβ1-AR/Gs/GRKs/β-arrestin signaling deflection system via multiple pathways.
基金supported by the National Natural Science Foundation of China(No.81802036 and No.81876182).
文摘Objective This study aimed to investigate the therapeutic effects and underlying mechanisms of the combination of Yinchenhao decoction(YCHD)and praziquantel(PZQ)in a Schistosoma japonicum(S.japonicum)-induced mouse model of schistosomiasis.Methods Six-week-old male BALB/c mice were randomly divided into five groups:control group,infected group,infected-PZQ group(I-PZQ),infected-YCHD group(I-YCHD),and infected-PZQ+YCHD group(I-PZQ+YCHD).The mice were infected with S.japonicum cercariae in infected group,I-PZQ group,I-YCHD group,and I-PZQ+YCHD group(n=6 per group)and maintained for 63 days.From day 43 to day 63 postinfection,the mice received PZQ(150 mg/kg,intragastric gavage),YCHD(10 mL/kg,intragastric gavage),or a combination of both.The control and infected groups received equal amounts of sterile double-distilled water for the same period.At the end of the experiment,the mice were anesthetized with pentobarbital sodium and sacrificed.Serum alanine transaminase(ALT)and aspartate transaminase(AST)levels were measured.Network pharmacology analysis was used to predict the targets of YCHD in the treatment of schistosomiasis.Histopathological analysis,Western blotting,immunofluorescence,quantitative polymerase chain reaction and flow cytometry were employed to evaluate liver pathology and molecular changes.Results Compared with the other groups,the I-PZQ+YCHD group presented significantly decreased serum ALT and AST levels(P<0.001).The I-PZQ+YCHD group exhibited improved pathological changes in the liver,as evidenced by reduced area of single granuloma(P<0.01),granuloma area(P<0.01),and Ishak score of liver fibrosis(P<0.01).Network pharmacology analysis suggested that YCHD may alleviate schistosomiasis-related liver injury through the modulation of the endoplasmic reticulum stress(ERS)pathway.Western blot analysis revealed that ERS-related markers,including glucose-regulated protein 78(GRP78),inositol-requiring enzyme 1 alpha(IRE1α),X-box binding protein 1(XBP-1),and C/EBP homologous protein(CHOP),were significantly downregulated in the I-PZQ+YCHD group(P<0.05).Furthermore,the I-PZQ+YCHD group presented reduced hepatocyte apoptosis(P<0.05),diminished hepatic macrophage infiltration(P<0.05)and downregulated expression of proinflammatory cytokines(TNF-α,IL-1βand IL-6)(P<0.05).Conclusion YCHD combined with PZQ reduced schistosomiasis-associated hepatic granulomatous inflammation and fibrosis by inhibiting hepatic apoptosis and ERS.
基金Supported by Traditional Chinese Medicine Science and Technology Development Plan of Jiangsu Province (No. MS2021013, ZD202215):Exploring the Efficacy and Mechanism of Professor Cao Shihong's Shenji Guben Decoction in Treating Chronic Obstructive Pulmonary Disease Based on Metabolomicsa Real-World Cohort Study on Traditional Chinese Medicine for the Treatment of Acute Exacerbation of Chronic Obstructive Pulmonary DiseaseIntra-hospital Fund of Jiangsu Provincial Hospital of Traditional Chinese Medicine Development (No. Y2021ZR11):Investigating the Mechanism of Shenji Guben Decoction in Treating Chronic Obstructive Pulmonary Disease Based on Metabolomics
文摘OBJECTIVE:To investigate the mechanisms of the effect of Shenji Guben(SJGB)decoction(参吉固本方)on chronic obstructive pulmonary disease(COPD).METHODS:A murine model of COPD was established through lipopolysaccharide(LPS)nasal drops and passive smoke exposure,followed by evaluation of SJGB decoction efficacy via lung function tests and histological analysis.Non-targeted liquid chromatography-mass spectrometry(LC-MS)-based metabolomics was used to explore the mechanisms of SJGB decoction in COPD.RESULTS:We found that the SJGB decoction effectively reduced inflammatory cell infiltration in the airways and lungs,and improved lung function in the COPD model mice.LC-MS-based metabolomics identified 86 biomarkers in COPD models.Compared to the model group,SJGB decoction significantly altered 34 metabolites.Prostaglandin E2 and DL-Citrulline were highlighted as two representative differential metabolites.MetaboAnalyst 5.0 highlighted glycerophospholipid and riboflavin metabolisms as key pathways affected by SJGB decoction.CONCLUSION:This study evaluated the protective effect of SJGB decoction against COPD and provided insights into its potential mechanisms in COPD treatment.
基金supported by the Hebei Provincial Administration of Traditional Chinese Medicine 2022 Chinese medicine research program mandatory subject(2021.No.12)Hebei famous traditional Chinese medicine inheritance studio construction project(2024.No.37)Yunnan Fundamental Research Projects(grant No.202501AT070266).
文摘Background:Diabetic kidney disease(DKD)is a major cause of end-stage renal disease,with limited effective treatment options currently available.Shenqi Dihuang Decoction(SQDH)has demonstrated clinical efficacy in managing DKD;however,the metabolic mechanisms responsible for its therapeutic effects remain unclear.Methods:We established a DKD mouse model and treated the mice with SQDH to investigate its effects on renal function and tissue pathology.To explore the metabolic mechanisms,we conducted non-targeted metabolomics to identify differential metabolites in the renal tissues of DKD mice and the associated metabolic pathways affected by SQDH.Additionally,we performed RT-qPCR and Western blot analyses to assess the effects of SQDH on the expression of key genes and proteins within the targeted pathways.To further evaluate SQDH’s therapeutic effects,we measured oxidative stress markers and inflammatory factors,examining its antioxidant and anti-inflammatory properties in DKD.Results:SQDH treatment improved body weight and blood glucose levels in DKD mice.It also restored renal function,as indicated by improved 24h-UTP,serum creatinine,and blood urea nitrogen levels,and alleviated renal tissue pathology associated with DKD.Metabolomic analysis showed that SQDH primarily regulates glycerophospholipid metabolism,particularly by increasing phosphatidylcholine(PC)levels and decreasing lysophosphatidylcholine(LPC)levels.RT-qPCR and Western blot analyses revealed that SQDH upregulated LPCAT expression and downregulated PLA2G expression.Additionally,SQDH enhanced the activities of superoxide dismutase and glutathione peroxidase,reduced reactive oxygen species,4-hydroxy-2-nonenal,and malondialdehyde levels,and decreased the levels of inflammatory cytokines IL-1β,IL-6,and TNF-α.Conclusion:Our findings confirm that SQDH protects against DKD by regulating glycerophospholipid metabolism,restoring the balance of PC and LPC,inhibiting inflammatory responses,and reducing oxidative stress.
基金supported by the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No.ZYYCXTD-D-202209)the National Natural Science Foundation of China(NSFC)Youth Program(No.82405145)the National Postdoctoral Researcher Support Program Category C(No.GZC20230335).
文摘Objectives:Irinotecan(CPT-11)-induced delayed diarrhea is a major dose-limiting toxicity that restricts its clinical application.Gegen Qinlian decoction(GQD),a traditional Chinese herbal formula,has shown potential in alleviating CPT-11-induced intestinal injury,but its underlying mechanisms remain incompletely understood.This study aimed to systematically elucidate the protective effects and mechanisms of GQD against CPT-11 enterotoxicity.Methods:We integrated ultra-performance liquid chromatography-quadrupole-Orbitrap-mass spectrometry(UPLC-QE-Orbitrap-MS)for chemical profiling of GQD,along with network pharmacology,molecular docking,and molecular dynamics simulation to identify key bioactive constituents and potential targets.Furthermore,a CPT-11-treated mouse model and Caco-2 cell monolayers were employed to evaluate the effects of GQD.Quantitative proteomics and untargeted metabolomics were applied to explore pathway alterations,and biochemical,histological,and functional analyses were conducted to assess inflammatory responses,oxidative stress,barrier integrity,and drug transporter activity.Results:A total of 65 compounds were identified in GQD,with 17 prioritized as putative bioactive constituents.Network analysis highlighted the IL-6/IL-22/STAT3 inflammatory axis and ABC transporter pathways as central mechanisms.In vivo,GQD administration ameliorated diarrhea,preserved colonic architecture and mucin production,reduced pro-inflammatory cytokines and oxidative stress,and restored expression of tight-junction proteins.Multi-omics integration showed that GQD counteracted CPT-11-induced dysregulation in complement/coagulation cascades and metabolic pathways,while enriching ABC transporter-related functions.In Caco-2 monolayers,GQD promoted SN-38 efflux,upregulated MDR1 and MRP2 expression,and decreased intracellular SN-38 accumulation.Consistent with this,GQD enhanced colonic clearance of SN-38 in mice.Conclusions:GQD protects against CPT-11-induced enterotoxicity primarily through two mechanisms:suppressing the pro-inflammatory IL-6/IL-22/STAT3 signaling pathway and enhancing the epithelial efflux of SN-38 via upregulation of ABC transporters.These findings provide a mechanistic basis for the potential use of GQD as an adjuvant therapy to mitigate intestinal toxicity during irinotecan chemotherapy.
基金Supported by Liaoning Provincial Science and Technology Plan Joint Plan,No.2023JH2/101700149。
文摘BACKGROUND The deleterious effects of surgical trauma and subsequent postoperative complications pose significant challenges to the smooth recovery of patients after gastric cancer(GC)resection despite the substantial curative benefits provided by surgical interventions for GC.Hence,the investigation of more optimal and efficacious treatment approaches has become an urgent necessity in the medical community.AIM To investigate the association of Sijunzi decoction plus chemotherapy with the gastrointestinal function and serum markers of patients after GC surgery.METHODS This study included patients who underwent GC surgery from June 2022 to February 2024.The control group included 45 patients who received chemotherapy(oxaliplatin+calcium folinate+5-fluorouracil),whereas the research group consisted of 54 patients who received Sijunzi decoction therapy in addition to the treatment administered in the control group.Comparative analyses were conducted from the following perspectives:Gastrointestinal function(defecation time,intestinal gas discharge time,and hospitalization time),serum markers[carcinoembryonic antigen(CEA),carbohydrate antigen(CA)125,and CA199],nutritional indicators total protein(TP)and transferrin(TRF),traditional Chinese medicine(TCM)syndrome score,and grades Ⅲ–Ⅳ adverse events(gastrointestinal reactions,renal/liver function impairment,and myelosuppression).RESULTS The two groups demonstrated similar defecation time(P>0.05),but the intestinal gas discharge time and hospitalization time were significantly shortened in the research group(P<0.05).Further,the research group exhibited significant CEA,CA125,and CA199 reductions after treatment,which were lower compared to the control group,as well as notable increases in TP and TRF that were statistically higher than the control group(all P<0.05).Furthermore,the research group demonstrated an evident decrease in TCM syndrome scores in areas,such as poor appetite,epigastric distension and pain,fatigue and weakness(P<0.01),and abdominal distension after eating,which are notably lower than those in the control group(P<0.01),with a comparable incidence of grades Ⅲ-Ⅳ adverse events(P>0.05).CONCLUSION Our research results indicate that Sijunzi decoction plus chemotherapy exerts a good rehabilitation-promoting effect on gastrointestinal function in patients after GC surgery and significantly downregulates abnormally increased CEA,CA125,and CA199 levels.
基金supported by the National Natural Science Foundation of China(No.82360897)Natural Science Foundation of Jiangxi Province of China(No.20212BAB216007)Administration of Traditional Chinese Medicine of Jiangxi Province of China(No.2022A328).
文摘Background:Damp-heat syndrome(DHS)is a complex condition in traditional Chinese medicine(TCM)that can cause various issues in circulation,digestion,and the respiratory system.This syndrome is believed to be closely linked to environmental factors such as high temperature and high humidity environments.Tingzhen Lu,a prominent physician during the Qing Dynasty,proposed the efficacy of Huanglian Wengdan Decoction(HLWDT)in addressing ailments stemming from high-temperature and high-humidity conditions.Nevertheless,the specific therapeutic effects of this decoction on DHS and its underlying mechanisms remain incompletely understood.Methods:To clarify the composition of HLWDT,mass spectrometry was utilized to identify the constituent compounds.Moreover,DHS rats induced by high humidity-temperature combined with a high sugar-fatty diet were treated with Huanglian Wendan decoction,the efficacy of which was evaluated based on serum biochemical indices and histopathological analyses.The expression of corresponding proteins was verified using western blotting.The mechanism of DHS relief by HLWDT was investigated by integration of network pharmacology and non-targeted metabolomics.Results:The HLWDT contained nearly 1,315 ingredients,the majority of which were flavonoids.Moreover,HLWDT not only regulated gastrointestinal motility and oxidative stress in DHS rats but also alleviated their inflammatory state.Metabolomics and network pharmacology analysis revealed that HLWDT primarily affects bile secretion,linoleic acid metabolism,PPAR,and MAPK signaling pathways.Furthermore,the PPAR signaling pathway was confirmed.HLWDT decreased the expression of NF-κB p65 and promoted MAPK phosphorylation and PPARγexpression in DHS rats.Conclusion:The therapeutic effect of HLWDT on DHS is potentially attributable to activation of the PPARγ-NF-κB/MAPK signaling pathway and regulation of oxidative stress and inflammatory responses.This experiment preliminarily elucidated the impact and mechanisms of HLWDT on DHS through pharmacodynamics,network pharmacology,and metabolomics.
基金Supported by National Natural Science Foundation of China:Study on the Mechanism of Action of Angelica Sinensis and Paeonia Lactiflora Powder in Activating Blood and Inducing Diuresis by Interfering with Microglia Polarization to Promote the Repair of White Matter Injury after Stroke (No. 82274401)Regulation of Calcium Homeostasis in Myocardial Ischemia/reperfusion Injury by Effective Components of Ginseng and Sorbus Soup and Its Mechanisms (No. 81473591)
文摘OBJECTIVE:To explore the active compounds and the mechanism of Shenfu decoction(参附汤,SFD)against ischemic stroke(IS)through network pharmacology and animal experiments.METHODS:SFD components were retrieved from the Traditional Chinese Medicine(TCM)database.The Online Mendelian Inheritance in Man(OMIM),Comparative Toxicogenomics Database(CTD)and Therapeutic Target Database(TTD)database were used to retrieve the IS-related disease targets.The herb-compound-target network was built by Cytoscape 3.7.1 software.The core targets were obtained using protein-protein interaction(PPI)network.The core targets of SFD were further analyzed through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).We then performed molecular docking between the hub proteins and key active compounds.Finally,we conducted animal experiments to verify the regulation of SFD on apoptosis following IS.RESULTS:There were 221 corresponding targets and 25 components related to Chinese medicine throughout the compound-target network.The core targets of SFD in the treatment of IS was tumor protein P53(Tp53),mitogen-activated protein kinase 3(MAPK3),MAPK1,heat shock proteins 90AA1 and alpha serine/threonine-protein kinase1.There were 221 GO items in GO function enrichment analysis and 106 signaling pathways in KEGG,mainly including negative regulation of the apoptosis process,vascular endothelial growth factor signaling pathways,NOD-like receptor signaling pathway,etc.Among them,Tp53,MAPK3,and MAPK1 were docked with small molecule compounds.Through animal research,we confirmed the effect of SFD on apoptosis following stroke.CONCLUSION:This study demonstrates that SFD can treat IS through multiple targets and pathways,and provides new perspectives for exploring the core targets and mechanisms of SFD against IS.
文摘Objective:To investigate the clinical efficacy of Xiaochaihu Decoction combined with Xiaoxianxiong Decoction in the treatment of post-stroke pneumonia.Methods:To complete the sample grouping comparison,all patients with post-stroke pneumonia were investigated,and the number of cases was 60.These patients’diseases were consistent with the dialectical standards of traditional Chinese medicine(phlegm-heat obstructing lungs).The patients were randomly divided into a control group(30 cases,treated with antibiotics and symptomatic methods)and a treatment group(30 cases,treated with Xiaochaihu Decoction and Xiaoxianxiong Decoction on the basis of the control group).Various indicators were compared.Results:The total clinical effective rates were 93%and 80%in the treatment group and the control group,respectively,with statistical significance(P<0.05).The improvement of various clinical symptoms was compared,and the values in the treatment group were reduced,showing significance(P<0.05).Analysis of serum factor indicators showed that the overall trend of the treatment group was reduced,and the comparison between groups was below 0.05.Conclusion:Xiaochaihu Decoction combined with Xiaoxianxiong Decoction has a significant clinical effect in the treatment of post-stroke pneumonia(phlegm-heat obstructing lungs syndrome),which can reduce inflammatory reactions and has few adverse reactions,worthy of clinical application.
基金Supported by the Key Program of Shandong Province,China,No.2016CYJS08A01-6.
文摘BACKGROUND In China Banxia Xiexin decoction(BXD)has been used in treating gastric cancer(GC)for thousands of years.BXD has been shown to reverse GC histopathology,but its chemical composition and action mechanism are still unknown.AIM To investigate the mechanism of BXD against GC based on utilizing transcrip-tomics and proteomics techniques experiments.METHODS Using the AGS cell line as the model group,the Cell Counting Kit-8 method and Annexin V-AbFluor™were employed 488/propidium iodide double staining method was used to detect the levels of cell proliferation and apoptosis.Differ-ential expression genes and differentially expressed proteins before and after BXD intervention were detected using RNA-seq and Pro DIA techniques.Key tran-scription factors were identified by enrichment and analysis using Metascape,and the key pathways were validated by western blot and reverse transcription PCR in vitro and in vivo experiments.RESULTS BXD significantly inhibited the proliferation rate and migration rate of GC cells and promoted cell apoptosis.The comprehensive analysis of transcriptomics and proteomics showed that five transcription factors,namely phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha,phosphoinositide-3-kinase regulatory subunit 1,AKT serine/threonine kinase 1,heat shock protein 90 alpha family class A member 1,and tumor protein p53,were key factors in BXD-mediated anti-cancer therapy and participated in the phosphatidylinositol 3-kinase(PI3K)/AKT signaling pathway.In vitro experiments were conducted using LY294002,an inhibitor of the PI3K/AKT signaling pathway,to validate the expression of five transcription factors at the protein and mRNA levels.In vivo experiments have shown that BXD inhibits tumor growth and suppresses the expression of the PI3K/AKT signaling pathway.CONCLUSION Transcriptomic and proteomic analysis showed that BXD inhibited tumor growth and slowed cancer progression by suppressing five factors in the PI3K/AKT signaling pathway,including phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha,phosphoinositide-3-kinase regulatory subunit 1,and AKT serine/threonine kinase 1.
基金supported by National Nature Science Foundation of China(Grant Nos.:82320108022,82322076,and 822104466)Shanghai Rising-Star Program,China(Grant No.:20QA1409300)+1 种基金the National Funding Program for Postdoctoral Researchers,China(C gradeProgram No.:GZC20231707).
文摘Colorectal inflammatory cancer transformation poses a major risk to patients with colitis.Patients with chronic intestinal inflammation have an approximately 2–3 fold increased risk of developing colorectal cancer(CRC).Unfortunately,there is currently no effective intervention available.Huangqin decoction(HQD),a well-known traditional Chinese medicine(TCM)formula,is frequently clinically prescribed for treating patients with colitis,and its active ingredients have effective antitumour efficacy.Nonetheless,the mechanism of HQD-mediated prevention of colorectal inflammatory cancer transformation remains unclear.A strategy integrating metagenomic,lipidomic,and messenger RNA(mRNA)sequencing analysis was used to investigate the regulatory effects of HQD on the gut microbiome,metabolism and potential mechanisms involved in colorectal inflammatory cancer transformation.Our study revealed that HQD suppressed colorectal inflammatory cancer transformation,which was associated with enhanced intestinal barrier function,decreased the inflammatory response,and regulation of the gut microbiome.Notably,cohousing experiments revealed that the transfer of the gut microbiome from HQD-treated mice largely inhibited the pathological transformation of colitis.Moreover,gut microbiome transfer from HQD-treated mice primarily resulted in the altered regulation of fatty acid metabolism,especially the remodeling of arachidonic acid metabolism,which was associated with the amelioration of pathological transformation.Arachidonic acid metabolism and the key metabolic enzyme arachidonic acid 12-lipoxygenase(ALOX12)were affected by HQD treatment,and no obvious protective effect of HQD was observed in Alox12−/−mice,which revealed that ALOX12 was a critical mediator of HQD protection against colorectal inflammatory cancer transformation.In summary,multiple omics analyses were applied to produce valuable data and theoretical support for the application of HQD as a promising intervention for the transformation of inflammatory CRC.
