Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic effi...Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic efficacy was evaluated.In the DIV1 challenge experiment,the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group,with the viral load in deceased individuals exceeding 5.41×10^(7)copies/μg-DNA.The thermal treatment(TT)was administered at 36℃for a duration of 16 d,followed by a temperature restoration(TR)period at 26℃for 3 d.On the first day at 36℃,an average viral concentration of 5.34×10 copies/μg-DNA was detected in the survived individuals.RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway,including sialin-like isoform x2(slc17a5),beta-galactosidase-1-like protein 2(glb1),putative glucosylceramidase 3(gba),sphingomyelin phosphodiesterase-like isoform x2(smpd1),betahexosaminidase subunit alpha-like(hexa_b)and legumain-like protein(lgmn),following a transient suppression period induced by thermal stress.Upon reaching 36℃,the activation of heat shock protein 70(hsp70)and heat shock protein 90(hsp90a)was observed.Concomitantly,genes that implicated in energy production critical for DIV1 replication,such as hexokinase(hk)and microsomal glutathione stransferase 3-like isoform x2(gst),were inhibited.These results collectively suggest that TT/TR treatments eliminated DIV1 in M.rosenbergii by activating the organism’s innate immune response and suppressing virus replication.This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in M.rosenbergii breeding programs,thereby facilitating the development of new strains resistant to DIV1.展开更多
The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries ...The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries of Central Europe,is also reported for the first time for Italy.This crayfishseems to become well established in the Iseo Lake(Northern Italy).The Author underlines the risk of a possibleterritorial expansion of this species in the Po river drainage.展开更多
The Luoping Biota discovered from the early Middle Triassic （Anisian） Guanling Formation of southwestern China represents a fully recovered shallow marine ecosystem, marking the end point of Early Triassic biotic re...The Luoping Biota discovered from the early Middle Triassic （Anisian） Guanling Formation of southwestern China represents a fully recovered shallow marine ecosystem, marking the end point of Early Triassic biotic recovery following the end-Permian mass extinction. Contemporaneously preserved are prolific trace fossils, which offer good opportunities to understand the palaeoecology of marine invertebrates from a fully recovered shallow marine ecosystem. Here we present a newly discovered sinuous branching burrow from the fossil-bearing unit in Member II of the Guanling Formation. Several features, including the horizontal regular sinuous nature, the branching pattern, typical H-junction, and the small wavelength/amplitude ratio of these sinuous structures within the burrow systems justify assignment of these traces as Sinusichnus sinuosus, a trace possibly produced by decapod crustaceans. Close association ofS. sinuosus with Rhizocorallium commune suggests a deposit-feeding strategy of these trace makers. The newly reported Anisian material from the Guanling Formation in Luoping represents first report of Sinusichnus from South China. The global record of Sinusichnus occurrence suggests that these burrows might have an older history than Early Middle Triassic.展开更多
Despite their key role as model organisms in many behavioral studies, crustacean decapods have been only slightly touched upon by the recent surge of scientific interest in animal personality. Only seven articles inve...Despite their key role as model organisms in many behavioral studies, crustacean decapods have been only slightly touched upon by the recent surge of scientific interest in animal personality. Only seven articles investigated the issue in a handful of species among hermit crabs, crabs, and crayfish. Obviously, a limited number of publications does not mean that personality is rare in decapods. On the contrary, few studies might be the result of a form of reluctance by behavioral ecologists to deal with such a phenomenon in these and other invertebrates. This reluctance contrasts with the enthusiasm shown in tackling the beha- vioral plasticity issue. Here we discuss the possible theoretical and methodological difficulties raised by applying the animal per-sonality perspective to decapods and analyze implications of personality studies for their ecology, conservation, and welfare. By highlighting gaps in knowledge and directions of future research, our intention is to increase scientific emphasis on the issue.展开更多
The study was to find out the effect of cadmium and mercury on levels of hemolymph glucose, crustacean hyperglycemic hormone（CHH） and hepatopancreas glycogen in the blue swimmer crab Portunus pelagicus. The experime...The study was to find out the effect of cadmium and mercury on levels of hemolymph glucose, crustacean hyperglycemic hormone（CHH） and hepatopancreas glycogen in the blue swimmer crab Portunus pelagicus. The experiments were performed in both intact and eyestalk ablated crabs. Quantification of CHH was done by the indirect ELISA with the aid of primary anti-Carcinus maenas-CHH antibody. Higher glucose concentration was observed on exposure to 8×10^–6 of cadmium（（825.6±5.42） μg/m L） and 6×10^–6 of mercury（（90.5±6.25） μg/m L）after 48 h and 24 h respectively. Higher level of hemolymph glucose was observed in eyestalk intact crabs on exposure to cadmium and mercury than eyestalk ablated crabs. Decrease in the levels of CHH was observed in both eyestalk intact and ablated crabs on heavy metal exposure. Decline of the hepatopancreas glycogen level was also witnessed with the exposure to heavy metal, which validated its utilization in the production of glucose. Thus this study brings to light, the variations in hemolymph glucose, CHH and hepatopancreas glycogen on heavy metal stress. These carbohydrate metabolites can be used as biomarkers in assessing heavy metal contamination in water bodies.展开更多
[Objective]The paper briefly summarizes the gene cloning and molecular research progress of vitellogenin in crustaceans belonging to Decapod.[Method]The molecular characteristics of identified Vg proteins in crustacea...[Objective]The paper briefly summarizes the gene cloning and molecular research progress of vitellogenin in crustaceans belonging to Decapod.[Method]The molecular characteristics of identified Vg proteins in crustacean are reviewed from the aspects of protein synthesis,molec-ular structure,phylogenetic evolution,hormonal regulation of gene expression and biological functions.[Result]The similarity of structure and func-tion in homologous protein are pointed out;phylogenetic relationships among molecules are analyzed;the main characteristics of Vg interspecific protein in Decapod are summarized,and the special parts different from other species are raised.[Conclusion]The study can offer some help for the further study of Vg gene,and provides theoretical basis for ovary development mechanism of crabs.展开更多
RNA interference(RNAi)has emerged as a key molecular tool in various commercially important decapod crustaceans,offering potential biotechnological applications in aquaculture.However,in the tropical rock lobster Panu...RNA interference(RNAi)has emerged as a key molecular tool in various commercially important decapod crustaceans,offering potential biotechnological applications in aquaculture.However,in the tropical rock lobster Panulirus ornatus,gene silencing through RNAi has proven difficult to achieve despite the availability of extensive omics data.This study investigates the RNAi response across life stages in P.ornatus,focusing on larvae and juveniles to determine when the species is most receptive to RNAi.Late-stage phyllosoma larvae and early juveniles were microinjected with dsRNA for the insulin-like growth factor binding protein encoding gene to determine silencing efficiency.Our results show that while juveniles exhibit an efficient systemic RNAi response with robust silencing across tissues,larvae display limited silencing capacity.A key finding is the differential expression of RNAi pathway components,including SID1,which facilitates dsRNA uptake in juveniles but is less active in larvae.Fluorescent microscopy revealed that dsRNA is rapidly sequestered and expelled by the antennal gland in larvae,potentially limiting RNAi efficacy.To further explore the mechanisms underlying RNAi in P.ornatus,RNA-seq analysis was conducted on pleopods collected across time points after dsRNA exposure in juvenile lobsters.Transcriptomic analysis identified significant upregulation of RNAi machinery,including Dicer-2,Argonaute-2,and SID1,which are critical for silencing.Additionally,several genes associated with antiviral responses were differentially expressed,suggesting broader involvement of RNAi in immune regulation.These findings highlight the potential to enhance RNAi strategies in P.ornatus juveniles,advancing the development of RNAi-based tools for disease resistance and productivity in aquaculture.展开更多
Portunids are important to recreational fisheries in Australia and typically are harvested by baited gears.Most of these baited gears are considered environmentally benign,although small conical hoop(tangle)nets fishe...Portunids are important to recreational fisheries in Australia and typically are harvested by baited gears.Most of these baited gears are considered environmentally benign,although small conical hoop(tangle)nets fished in southeastern Australia sometimes are damaged and lose twine,which becomes marine debris.Substituting conventional multifilament polyamide(PA)twine with alternative multi-monofilament and monofilament PA twines in hoop nets can dramatically reduce marine debris,but there remain unanswered questions concerning the relative fishing power of alternative twines among derelict hoop nets.This study addresses the shortfall in data by comparing the efficiencies and damage to replicate hoop nets made from the three twines and left to fish for short(one and two days),medium(three to five days)and long(ten and fourteen days)soaks.Irrespective of twine material or soak time,all hoop nets entangled the same numbers of giant mud crabs,Scylla serrata implying catch saturation within two days.Only four other animals were caught(all yellowfin bream,Acanthopagrus australis)and all died.At least some replicates of all hoop nets were damaged and lost twine,but this was greatest among the multifilament(up to a maximum of 9.3m hoop-net deployment−1),followed by the multi-monofilament(up to 7.4m hoop-net deployment−1)and monofilament hoop nets(1.4m hoop-net deployment−1).Replacing the existing multifilament twine in hoop nets with monofilament when targeting S.serrata would maintain catches and minimise marine debris during conventional soaks,without increasing ghost fishing over the short term.展开更多
基金Supported by the earmarked fund for CARS(No.CARS-48)the National Natural Science Foundation of China(No.32202964)。
文摘Decapod Iridescent Virus 1(DIV1)is a recently discovered virus recognized for its high infectivity in Macrobrachium rosenbergii.A thermal treatment was performed on DIV1-infected M.rosenbergii,and the therapeutic efficacy was evaluated.In the DIV1 challenge experiment,the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group,with the viral load in deceased individuals exceeding 5.41×10^(7)copies/μg-DNA.The thermal treatment(TT)was administered at 36℃for a duration of 16 d,followed by a temperature restoration(TR)period at 26℃for 3 d.On the first day at 36℃,an average viral concentration of 5.34×10 copies/μg-DNA was detected in the survived individuals.RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway,including sialin-like isoform x2(slc17a5),beta-galactosidase-1-like protein 2(glb1),putative glucosylceramidase 3(gba),sphingomyelin phosphodiesterase-like isoform x2(smpd1),betahexosaminidase subunit alpha-like(hexa_b)and legumain-like protein(lgmn),following a transient suppression period induced by thermal stress.Upon reaching 36℃,the activation of heat shock protein 70(hsp70)and heat shock protein 90(hsp90a)was observed.Concomitantly,genes that implicated in energy production critical for DIV1 replication,such as hexokinase(hk)and microsomal glutathione stransferase 3-like isoform x2(gst),were inhibited.These results collectively suggest that TT/TR treatments eliminated DIV1 in M.rosenbergii by activating the organism’s innate immune response and suppressing virus replication.This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in M.rosenbergii breeding programs,thereby facilitating the development of new strains resistant to DIV1.
文摘The American Striped Cray Orconectes limosus(Rafinesque),a nearctic crustacean new in theitalian freshwaters(Crustacea Decapoda Cambaridae).The American Striped Cray Orconectes limosus,introduced in several countries of Central Europe,is also reported for the first time for Italy.This crayfishseems to become well established in the Iseo Lake(Northern Italy).The Author underlines the risk of a possibleterritorial expansion of this species in the Po river drainage.
基金partly supported by the ARCDP discoverygrant to G. R. Shi (No. DP150100690)supported by a CAS Pioneer Hundred Talents Program (to ML)+2 种基金supported by a grant from the State Key Laboratory of Biogeology and Environmental Biology (No. GBL21410 to ML), China University of Geosciences (Wuhan)a grant from the National Natural Science Foundation of China (No. 41502013)supported by for China Geological Survey projects (Nos. DD20160020, 1212011140051, 12120114030601, and 1212010610211)
文摘The Luoping Biota discovered from the early Middle Triassic （Anisian） Guanling Formation of southwestern China represents a fully recovered shallow marine ecosystem, marking the end point of Early Triassic biotic recovery following the end-Permian mass extinction. Contemporaneously preserved are prolific trace fossils, which offer good opportunities to understand the palaeoecology of marine invertebrates from a fully recovered shallow marine ecosystem. Here we present a newly discovered sinuous branching burrow from the fossil-bearing unit in Member II of the Guanling Formation. Several features, including the horizontal regular sinuous nature, the branching pattern, typical H-junction, and the small wavelength/amplitude ratio of these sinuous structures within the burrow systems justify assignment of these traces as Sinusichnus sinuosus, a trace possibly produced by decapod crustaceans. Close association ofS. sinuosus with Rhizocorallium commune suggests a deposit-feeding strategy of these trace makers. The newly reported Anisian material from the Guanling Formation in Luoping represents first report of Sinusichnus from South China. The global record of Sinusichnus occurrence suggests that these burrows might have an older history than Early Middle Triassic.
文摘Despite their key role as model organisms in many behavioral studies, crustacean decapods have been only slightly touched upon by the recent surge of scientific interest in animal personality. Only seven articles investigated the issue in a handful of species among hermit crabs, crabs, and crayfish. Obviously, a limited number of publications does not mean that personality is rare in decapods. On the contrary, few studies might be the result of a form of reluctance by behavioral ecologists to deal with such a phenomenon in these and other invertebrates. This reluctance contrasts with the enthusiasm shown in tackling the beha- vioral plasticity issue. Here we discuss the possible theoretical and methodological difficulties raised by applying the animal per-sonality perspective to decapods and analyze implications of personality studies for their ecology, conservation, and welfare. By highlighting gaps in knowledge and directions of future research, our intention is to increase scientific emphasis on the issue.
基金The grant from the Department of Science and Technology,New Delhi under contract No.SR/FT/LS-137/2009 dt.17.01.2012
文摘The study was to find out the effect of cadmium and mercury on levels of hemolymph glucose, crustacean hyperglycemic hormone（CHH） and hepatopancreas glycogen in the blue swimmer crab Portunus pelagicus. The experiments were performed in both intact and eyestalk ablated crabs. Quantification of CHH was done by the indirect ELISA with the aid of primary anti-Carcinus maenas-CHH antibody. Higher glucose concentration was observed on exposure to 8×10^–6 of cadmium（（825.6±5.42） μg/m L） and 6×10^–6 of mercury（（90.5±6.25） μg/m L）after 48 h and 24 h respectively. Higher level of hemolymph glucose was observed in eyestalk intact crabs on exposure to cadmium and mercury than eyestalk ablated crabs. Decrease in the levels of CHH was observed in both eyestalk intact and ablated crabs on heavy metal exposure. Decline of the hepatopancreas glycogen level was also witnessed with the exposure to heavy metal, which validated its utilization in the production of glucose. Thus this study brings to light, the variations in hemolymph glucose, CHH and hepatopancreas glycogen on heavy metal stress. These carbohydrate metabolites can be used as biomarkers in assessing heavy metal contamination in water bodies.
基金funded by Special Fund for Agro-scientific Research in the Public Interest(201003070)Central Public-interest Scientific Institution Basal Research Fund(2011-JBFB02)
文摘[Objective]The paper briefly summarizes the gene cloning and molecular research progress of vitellogenin in crustaceans belonging to Decapod.[Method]The molecular characteristics of identified Vg proteins in crustacean are reviewed from the aspects of protein synthesis,molec-ular structure,phylogenetic evolution,hormonal regulation of gene expression and biological functions.[Result]The similarity of structure and func-tion in homologous protein are pointed out;phylogenetic relationships among molecules are analyzed;the main characteristics of Vg interspecific protein in Decapod are summarized,and the special parts different from other species are raised.[Conclusion]The study can offer some help for the further study of Vg gene,and provides theoretical basis for ovary development mechanism of crabs.
基金supported by the Australian Government with funding from the Australian Research Council(http://www.arc.gov.au/)Industrial Transformation Research Hub(project number IH190100014).
文摘RNA interference(RNAi)has emerged as a key molecular tool in various commercially important decapod crustaceans,offering potential biotechnological applications in aquaculture.However,in the tropical rock lobster Panulirus ornatus,gene silencing through RNAi has proven difficult to achieve despite the availability of extensive omics data.This study investigates the RNAi response across life stages in P.ornatus,focusing on larvae and juveniles to determine when the species is most receptive to RNAi.Late-stage phyllosoma larvae and early juveniles were microinjected with dsRNA for the insulin-like growth factor binding protein encoding gene to determine silencing efficiency.Our results show that while juveniles exhibit an efficient systemic RNAi response with robust silencing across tissues,larvae display limited silencing capacity.A key finding is the differential expression of RNAi pathway components,including SID1,which facilitates dsRNA uptake in juveniles but is less active in larvae.Fluorescent microscopy revealed that dsRNA is rapidly sequestered and expelled by the antennal gland in larvae,potentially limiting RNAi efficacy.To further explore the mechanisms underlying RNAi in P.ornatus,RNA-seq analysis was conducted on pleopods collected across time points after dsRNA exposure in juvenile lobsters.Transcriptomic analysis identified significant upregulation of RNAi machinery,including Dicer-2,Argonaute-2,and SID1,which are critical for silencing.Additionally,several genes associated with antiviral responses were differentially expressed,suggesting broader involvement of RNAi in immune regulation.These findings highlight the potential to enhance RNAi strategies in P.ornatus juveniles,advancing the development of RNAi-based tools for disease resistance and productivity in aquaculture.
基金funded by the NSW Department of Primary Industriesratified by the NSW Animal Care and Ethics Committee(ACEC ref no.08–06)。
文摘Portunids are important to recreational fisheries in Australia and typically are harvested by baited gears.Most of these baited gears are considered environmentally benign,although small conical hoop(tangle)nets fished in southeastern Australia sometimes are damaged and lose twine,which becomes marine debris.Substituting conventional multifilament polyamide(PA)twine with alternative multi-monofilament and monofilament PA twines in hoop nets can dramatically reduce marine debris,but there remain unanswered questions concerning the relative fishing power of alternative twines among derelict hoop nets.This study addresses the shortfall in data by comparing the efficiencies and damage to replicate hoop nets made from the three twines and left to fish for short(one and two days),medium(three to five days)and long(ten and fourteen days)soaks.Irrespective of twine material or soak time,all hoop nets entangled the same numbers of giant mud crabs,Scylla serrata implying catch saturation within two days.Only four other animals were caught(all yellowfin bream,Acanthopagrus australis)and all died.At least some replicates of all hoop nets were damaged and lost twine,but this was greatest among the multifilament(up to a maximum of 9.3m hoop-net deployment−1),followed by the multi-monofilament(up to 7.4m hoop-net deployment−1)and monofilament hoop nets(1.4m hoop-net deployment−1).Replacing the existing multifilament twine in hoop nets with monofilament when targeting S.serrata would maintain catches and minimise marine debris during conventional soaks,without increasing ghost fishing over the short term.
