Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells （BMSCs） toward the injured area. This study aimed to determine the...Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells （BMSCs） toward the injured area. This study aimed to determine the following： （1） the direction of rat BMSC （rBMSC） migration upon exposure to a direct current electric field （DCEF）, （2） the optimal DCEF intensity and duration, and （3） the possible regulatory role of SDF-1/ CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.展开更多
基金This study was supported by the Chinese Army Five-Year Project Funding （CWS 12J134） and the National Natural Science Foundation of China （No. 51473175）. We appreciate the Chinese Academy of Agricultural Sciences and the Institute of Orthopedics for their assistance for our experiments.
文摘Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells （BMSCs） toward the injured area. This study aimed to determine the following： （1） the direction of rat BMSC （rBMSC） migration upon exposure to a direct current electric field （DCEF）, （2） the optimal DCEF intensity and duration, and （3） the possible regulatory role of SDF-1/ CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.
