为了筛选有机溶剂DMSO与DMF对H9C2心肌细胞毒性作用的最低浓度,探讨DMSO与DMF对心肌细胞信号转导与转录因子3(signal transducer and activator of transcription3,STAT3)信号通路的影响,通过四氮唑盐比色分析法(MTT法)试验,检测DMSO和...为了筛选有机溶剂DMSO与DMF对H9C2心肌细胞毒性作用的最低浓度,探讨DMSO与DMF对心肌细胞信号转导与转录因子3(signal transducer and activator of transcription3,STAT3)信号通路的影响,通过四氮唑盐比色分析法(MTT法)试验,检测DMSO和DMF在体积分数为0.1%、0.2%、0.3%、0.5%、0.7%、0.9%、1%、2%、3%时作用24、48、72h心肌细胞的存活率变化;Westernblot法检测STAT3和Bcl-2蛋白,对比DMSO和DMF分别在0%、0.2%、0.3%、0.5%体积分数下STAT3蛋白表达量的差异性。结果表明,同一DMSO和DMF体积分数条件下,随着时间增加,细胞存活率下降,在同一时间随着DMSO和DMF体积分数的增加,心肌细胞存活率减小,Bcl-2和STAT3蛋白含量减少,DMSO体积分数为0.3%和0.5%时,细胞存活率有统计学意义(P<0.05),STAT3和Bcl-2蛋白水平比对照组低(P<0.01),但DMSO体积分数为0.2%,细胞存活率与STAT3、Bcl-2表达对比对照组,均无统计学意义(P>0.05);当DMF体积分数为0.3%和0.5%时,细胞存活率有统计学意义(P<0.05),STAT3和Bcl-2蛋白水平比对照组低(P<0.01),当DMF体积分数为0.2%时,细胞存活率无统计学意义(P>0.05),STAT3蛋白表达对比对照组,具有统计学意义(P<0.01)。与对照组(不含DMSO和DMF)比较,DMSO和DMF对H9C2心肌细胞具有毒性作用,随浓度增加,细胞存活率降低,Bcl-2蛋白含量降低,可能与STAT3蛋白下调相关,MTT法检测结果与Westernblot试验结果不完全一致,MTT法具有不稳定性。展开更多
在自制的1,8-二氮杂双环[5,4,0]十一碳-7-烯(DBU)吸收CO2的填料塔中,研究DBU溶液浓度、入口CO2浓度、DBU溶液流量、气体流量、气体温度以及填料层高度对CO2吸收的影响。结果表明,DBU溶液浓度从1.5%增加到25%,CO2吸收容量和吸收效率均逐...在自制的1,8-二氮杂双环[5,4,0]十一碳-7-烯(DBU)吸收CO2的填料塔中,研究DBU溶液浓度、入口CO2浓度、DBU溶液流量、气体流量、气体温度以及填料层高度对CO2吸收的影响。结果表明,DBU溶液浓度从1.5%增加到25%,CO2吸收容量和吸收效率均逐步减小;入口CO2浓度从4.5%增加到15%,CO2吸收容量和吸收效率均逐渐增大;DBU溶液流量从20 m L/min增加到120 m L/min,CO2吸收容量和吸收效率略有增加;气体流量从0.7 L/min增加到3.3 L/min,填料层高度从5 cm增加到45 cm,CO2吸收容量和吸收效率均呈先增后减趋势;气体温度从25℃增加到60℃,CO2吸收容量和吸收效率略有下降。DBU溶液在填料塔内能够高效吸收气体中的CO2。展开更多
All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA w...All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA were adopted to characterize the properties of tripeptide Ile-Ile-Pro in the MD simulations. Interestingly, the tripeptide molecule IIP exhibited different behaviors in aqueous and DMSO solutions. In aqueous solution, IIP was very flexible. The conformation could shift between extended and folded states very quickly. However,in DMSO solution, more folded conformations were observed. The interesting phenomena were proved by 2D-NOESY spectra.展开更多
文摘为了筛选有机溶剂DMSO与DMF对H9C2心肌细胞毒性作用的最低浓度,探讨DMSO与DMF对心肌细胞信号转导与转录因子3(signal transducer and activator of transcription3,STAT3)信号通路的影响,通过四氮唑盐比色分析法(MTT法)试验,检测DMSO和DMF在体积分数为0.1%、0.2%、0.3%、0.5%、0.7%、0.9%、1%、2%、3%时作用24、48、72h心肌细胞的存活率变化;Westernblot法检测STAT3和Bcl-2蛋白,对比DMSO和DMF分别在0%、0.2%、0.3%、0.5%体积分数下STAT3蛋白表达量的差异性。结果表明,同一DMSO和DMF体积分数条件下,随着时间增加,细胞存活率下降,在同一时间随着DMSO和DMF体积分数的增加,心肌细胞存活率减小,Bcl-2和STAT3蛋白含量减少,DMSO体积分数为0.3%和0.5%时,细胞存活率有统计学意义(P<0.05),STAT3和Bcl-2蛋白水平比对照组低(P<0.01),但DMSO体积分数为0.2%,细胞存活率与STAT3、Bcl-2表达对比对照组,均无统计学意义(P>0.05);当DMF体积分数为0.3%和0.5%时,细胞存活率有统计学意义(P<0.05),STAT3和Bcl-2蛋白水平比对照组低(P<0.01),当DMF体积分数为0.2%时,细胞存活率无统计学意义(P>0.05),STAT3蛋白表达对比对照组,具有统计学意义(P<0.01)。与对照组(不含DMSO和DMF)比较,DMSO和DMF对H9C2心肌细胞具有毒性作用,随浓度增加,细胞存活率降低,Bcl-2蛋白含量降低,可能与STAT3蛋白下调相关,MTT法检测结果与Westernblot试验结果不完全一致,MTT法具有不稳定性。
文摘在自制的1,8-二氮杂双环[5,4,0]十一碳-7-烯(DBU)吸收CO2的填料塔中,研究DBU溶液浓度、入口CO2浓度、DBU溶液流量、气体流量、气体温度以及填料层高度对CO2吸收的影响。结果表明,DBU溶液浓度从1.5%增加到25%,CO2吸收容量和吸收效率均逐步减小;入口CO2浓度从4.5%增加到15%,CO2吸收容量和吸收效率均逐渐增大;DBU溶液流量从20 m L/min增加到120 m L/min,CO2吸收容量和吸收效率略有增加;气体流量从0.7 L/min增加到3.3 L/min,填料层高度从5 cm增加到45 cm,CO2吸收容量和吸收效率均呈先增后减趋势;气体温度从25℃增加到60℃,CO2吸收容量和吸收效率略有下降。DBU溶液在填料塔内能够高效吸收气体中的CO2。
基金Supported by the National Natural Science Foundation of China(No.20903026)the Talents Introduction Foundation for Universities of Guangdong Province(2011)Scientific Research Foundation of the Natural Science Foundation of Guangdong Province(No.S2011010002483)
文摘All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA were adopted to characterize the properties of tripeptide Ile-Ile-Pro in the MD simulations. Interestingly, the tripeptide molecule IIP exhibited different behaviors in aqueous and DMSO solutions. In aqueous solution, IIP was very flexible. The conformation could shift between extended and folded states very quickly. However,in DMSO solution, more folded conformations were observed. The interesting phenomena were proved by 2D-NOESY spectra.
