Aim： To investigate the methylation status of the deleted in azoospermia 1（DAZ1） gene promoter region in different cell types. Methods： Using CpG island Searcher software, a CpG island was found in the promoter re...Aim： To investigate the methylation status of the deleted in azoospermia 1（DAZ1） gene promoter region in different cell types. Methods： Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results： The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm： it was methylated in leukocytes, but unmethylated in sperm. Conclusion： A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues. （Asian J Androl 2006 Jan; 8：61-67 ）展开更多
文摘Aim： To investigate the methylation status of the deleted in azoospermia 1（DAZ1） gene promoter region in different cell types. Methods： Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing. Results： The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm： it was methylated in leukocytes, but unmethylated in sperm. Conclusion： A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues. （Asian J Androl 2006 Jan; 8：61-67 ）
