目的:探讨TSLC1和DAL-1/4.1B两种蛋白在胰腺癌中的表达和临床病理意义.方法:采用免疫组织化学S-P法检测42例胰腺癌组织、11例胰腺炎组织和9例正常胰腺组织中TSLC1和DAL-1/4.1B两种蛋白的表达.结果:TSLC1、DAL-1/4.1B蛋白在胰腺癌组织中...目的:探讨TSLC1和DAL-1/4.1B两种蛋白在胰腺癌中的表达和临床病理意义.方法:采用免疫组织化学S-P法检测42例胰腺癌组织、11例胰腺炎组织和9例正常胰腺组织中TSLC1和DAL-1/4.1B两种蛋白的表达.结果:TSLC1、DAL-1/4.1B蛋白在胰腺癌组织中的阳性表达率均明显低于在正常胰腺组织和胰腺炎组织中的表达(30.95%vs77.78%,81.82%;28.57% vs 66.67%,81.82%,P<0.05或0.01).TSLC1和DAL-1/4.1B蛋白的异常表达均与胰腺癌的分化程度、淋巴结转移和TNM分期相关(P<0.05),而与患者的性别、年龄、部位和病理分型无关.在42例胰腺癌中TSLC1与DAL-1/4.1B蛋白表达呈显著正相关(rs=0.489,P<0.01).结论:胰腺癌中存在TSLC1和DAL-1/4.1B基因的失活和蛋白表达下调,二者可能通过TSLC1-DAL-1/4.1B级联反应共同参与胰腺癌的发生、发展和转移.展开更多
目的:研究肺癌肿瘤抑制物1(tumor suppressor i n lung cancer-1,TSLC1)、肺腺癌差异表达因子4.1B(differentially expressed in adenocarcinoma of the lung/4.1B,DAL-1/4.1B)和果蝇肿瘤抑制因子同源分子-3(membraneprotein palmitoyla...目的:研究肺癌肿瘤抑制物1(tumor suppressor i n lung cancer-1,TSLC1)、肺腺癌差异表达因子4.1B(differentially expressed in adenocarcinoma of the lung/4.1B,DAL-1/4.1B)和果蝇肿瘤抑制因子同源分子-3(membraneprotein palmitoylated 3,MPP3)在大肠癌中的表达及其相互关系,并探讨其临床病理意义.方法:采用免疫组织化学EnVisionTM法检测76例大肠癌和22例正常肠黏膜组织中TSLC1、DAL-1/4.1B、MPP3的表达,并结合其临床病理特征分析.结果:正常肠黏膜组织中TSLC1、DAL-1/4.1B和MPP3均呈清晰的棕黄色染色定位在上皮细胞质和/或细胞膜.TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌中的阳性表达率均明显低于其在正常肠黏膜组织中的表达(32.89%vs81.81%,27.63%vs 63.64%,35.53%vs 68.18%;P<0.05).TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌的表达缺失与肿瘤的分化程度、浸润深度、淋巴结转移和Dukes'分期密切相关(P<0.05),而与患者的性别、年龄和肿瘤大小无关.结论:大肠癌中存在TSLC1、DAL-1/4.1B和MPP3基因的表达缺失,TSLC1与DAL-1/4.1B及MPP3之间的相互作用可能是其发挥肿瘤抑制作用的主要分子机制,三者可能通过TSLC1级联反应共同参与大肠癌的发生、发展和转移.展开更多
肺癌肿瘤抑制物基因1（tumor suppressor of non—small cell lung cancer1／TSLC1）和肺腺癌差异表达因子4．1B（differentially expressed in adenocarcinoma of the lung／4．1B，DAL-1／4．1B）均是近年新发现的抑癌基因，具有潜在...肺癌肿瘤抑制物基因1（tumor suppressor of non—small cell lung cancer1／TSLC1）和肺腺癌差异表达因子4．1B（differentially expressed in adenocarcinoma of the lung／4．1B，DAL-1／4．1B）均是近年新发现的抑癌基因，具有潜在的肿瘤抑制作用。研究表明二者在多种组织中都有表达，而且在许多肿瘤中均表现为表达减低或表达缺失。肿瘤的浸润程度、转移程度和疾病预后情况与抑癌基因的表达与否密切相关，抑癌基因失活的主要可能机制之一是基因启动子发生甲基化。二者可作为新的肿瘤标志物，指导肿瘤的早期诊断及靶基因治疗。展开更多
Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms.The regulation of ageing pathways appears to differ between gymnosperms and angiosperms.We previousl...Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms.The regulation of ageing pathways appears to differ between gymnosperms and angiosperms.We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines.Therefore,elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development.Large-scale yeast two-hybrid(Y2H)analysis followed by next-generation high-throughput sequencing(Y2H-seq)allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis.Our study found that PtDAL1 interacting proteins showed an ageing-related module,with sophisticated interacting networks composed of transcription factors(TFs),transcriptional regulators(TRs),and kinases.These interacting proteins are produced in response to a variety of phytohormones and environmental signals,and are likely involved in wood formation,needle development,oleoresin terpenoids biosynthesis,and reproductive development.In this study,we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.展开更多
文摘目的:探讨TSLC1和DAL-1/4.1B两种蛋白在胰腺癌中的表达和临床病理意义.方法:采用免疫组织化学S-P法检测42例胰腺癌组织、11例胰腺炎组织和9例正常胰腺组织中TSLC1和DAL-1/4.1B两种蛋白的表达.结果:TSLC1、DAL-1/4.1B蛋白在胰腺癌组织中的阳性表达率均明显低于在正常胰腺组织和胰腺炎组织中的表达(30.95%vs77.78%,81.82%;28.57% vs 66.67%,81.82%,P<0.05或0.01).TSLC1和DAL-1/4.1B蛋白的异常表达均与胰腺癌的分化程度、淋巴结转移和TNM分期相关(P<0.05),而与患者的性别、年龄、部位和病理分型无关.在42例胰腺癌中TSLC1与DAL-1/4.1B蛋白表达呈显著正相关(rs=0.489,P<0.01).结论:胰腺癌中存在TSLC1和DAL-1/4.1B基因的失活和蛋白表达下调,二者可能通过TSLC1-DAL-1/4.1B级联反应共同参与胰腺癌的发生、发展和转移.
文摘目的:研究肺癌肿瘤抑制物1(tumor suppressor i n lung cancer-1,TSLC1)、肺腺癌差异表达因子4.1B(differentially expressed in adenocarcinoma of the lung/4.1B,DAL-1/4.1B)和果蝇肿瘤抑制因子同源分子-3(membraneprotein palmitoylated 3,MPP3)在大肠癌中的表达及其相互关系,并探讨其临床病理意义.方法:采用免疫组织化学EnVisionTM法检测76例大肠癌和22例正常肠黏膜组织中TSLC1、DAL-1/4.1B、MPP3的表达,并结合其临床病理特征分析.结果:正常肠黏膜组织中TSLC1、DAL-1/4.1B和MPP3均呈清晰的棕黄色染色定位在上皮细胞质和/或细胞膜.TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌中的阳性表达率均明显低于其在正常肠黏膜组织中的表达(32.89%vs81.81%,27.63%vs 63.64%,35.53%vs 68.18%;P<0.05).TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌的表达缺失与肿瘤的分化程度、浸润深度、淋巴结转移和Dukes'分期密切相关(P<0.05),而与患者的性别、年龄和肿瘤大小无关.结论:大肠癌中存在TSLC1、DAL-1/4.1B和MPP3基因的表达缺失,TSLC1与DAL-1/4.1B及MPP3之间的相互作用可能是其发挥肿瘤抑制作用的主要分子机制,三者可能通过TSLC1级联反应共同参与大肠癌的发生、发展和转移.
文摘肺癌肿瘤抑制物基因1（tumor suppressor of non—small cell lung cancer1／TSLC1）和肺腺癌差异表达因子4．1B（differentially expressed in adenocarcinoma of the lung／4．1B，DAL-1／4．1B）均是近年新发现的抑癌基因，具有潜在的肿瘤抑制作用。研究表明二者在多种组织中都有表达，而且在许多肿瘤中均表现为表达减低或表达缺失。肿瘤的浸润程度、转移程度和疾病预后情况与抑癌基因的表达与否密切相关，抑癌基因失活的主要可能机制之一是基因启动子发生甲基化。二者可作为新的肿瘤标志物，指导肿瘤的早期诊断及靶基因治疗。
基金supported by‘The National Natural Science Foundation of China(NO.31870651)’‘The Fundamental Research Funds for the Central Universities(NO.2021ZY55)’.
文摘Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms.The regulation of ageing pathways appears to differ between gymnosperms and angiosperms.We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines.Therefore,elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development.Large-scale yeast two-hybrid(Y2H)analysis followed by next-generation high-throughput sequencing(Y2H-seq)allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis.Our study found that PtDAL1 interacting proteins showed an ageing-related module,with sophisticated interacting networks composed of transcription factors(TFs),transcriptional regulators(TRs),and kinases.These interacting proteins are produced in response to a variety of phytohormones and environmental signals,and are likely involved in wood formation,needle development,oleoresin terpenoids biosynthesis,and reproductive development.In this study,we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.
