Tree peony is an emerging woody oilseed crop that has the potential to help meet the growing demand for edible oil and biofuel,and breeding high-yield varieties is the key to increasing production.The DA1 gene regulat...Tree peony is an emerging woody oilseed crop that has the potential to help meet the growing demand for edible oil and biofuel,and breeding high-yield varieties is the key to increasing production.The DA1 gene regulates seed weight and number and effectively improves crop yield,although its function in tree peonies has not yet been reported.In this study,three PrDA1 genes were first identified in the whole genome of‘Jing Hong'flare tree peony(Paeonia rockii).PrDA1-1 is homologous to AtDA1 and contains three types of conserved structural domains:UIM(ubiquitin-interacting motif),LIM(Lin11,Isl-1,and Mec-3),and DA-like.PrDA1-1 was strongly expressed during both the early and late stages of seed development,and its constitutive expression in Arabidopsis reduced seed number but increased seed weight,which ultimately led to a reduction in yield.PrDA1-1 was found to interact with PrTCP1/PrTCP9 in yeast and plants,and the constitutive expression of PrTCP1/PrTCP9 in Arabidopsis increased seed weight and number,which resulted in higher yield.Since this study showed that PrDA1-1 and PrTCP1/PrTCP9 regulate seed weight and number to affect yield,these new genetic resources will help to improve the seed yield of tree peonies.展开更多
Plant organ size is an important agronomic trait that makes a significant contribution to plant yield.Despite its central importance,the genetic and molecular mechanisms underlying organ size control remain to be full...Plant organ size is an important agronomic trait that makes a significant contribution to plant yield.Despite its central importance,the genetic and molecular mechanisms underlying organ size control remain to be fully clarified.Here,we report that the trithorax group protein ULTRAPETALA1(ULT1)interacts with the TEOSINTE BRANCHED1/CYCLOIDEA/PCF14/15(TCP14/15)transcription factors by antagonizing the LIN-11,ISL-1,and MEC-3(LIM)peptidase DA1,thereby regulating organ size in Arabidopsis.Loss of ULT1 function significantly increases rosette leaf,petal,silique,and seed size,whereas overexpression of ULT1 results in reduced organ size.ULT1 associates with TCP14 and TCP15 to co-regulate cell size by affecting cellular endoreduplication.Transcriptome analysis revealed that ULT1 and TCP14/15 regulate common target genes involved in endoreduplication and leaf development.ULT1 can be recruited by TCP14/15 to promote lysine 4 of histone H3 trimethylation at target genes,activating their expression to determinefinal cell size.Furthermore,we found that ULT1 influences the interaction of DA1 and TCP14/15 and antagonizes the effect of DA1 on TCP14/15 degradation.Collectively,ourfindings reveal a novel epigenetic mechanism underlying the regulation of organ size in Arabidopsis.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1200105)the National Natural Science Foundation of China(31972446)the 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University,China(BLRC2023A06)。
文摘Tree peony is an emerging woody oilseed crop that has the potential to help meet the growing demand for edible oil and biofuel,and breeding high-yield varieties is the key to increasing production.The DA1 gene regulates seed weight and number and effectively improves crop yield,although its function in tree peonies has not yet been reported.In this study,three PrDA1 genes were first identified in the whole genome of‘Jing Hong'flare tree peony(Paeonia rockii).PrDA1-1 is homologous to AtDA1 and contains three types of conserved structural domains:UIM(ubiquitin-interacting motif),LIM(Lin11,Isl-1,and Mec-3),and DA-like.PrDA1-1 was strongly expressed during both the early and late stages of seed development,and its constitutive expression in Arabidopsis reduced seed number but increased seed weight,which ultimately led to a reduction in yield.PrDA1-1 was found to interact with PrTCP1/PrTCP9 in yeast and plants,and the constitutive expression of PrTCP1/PrTCP9 in Arabidopsis increased seed weight and number,which resulted in higher yield.Since this study showed that PrDA1-1 and PrTCP1/PrTCP9 regulate seed weight and number to affect yield,these new genetic resources will help to improve the seed yield of tree peonies.
基金supported by the National Natural Science Foundation of China (31872805)the Fundamental Research Funds for Central NonProfit of the Chinese Academy of Agricultural Sciences (CAASZDRW202109 and Y2023PT20)the Nanfan Special Project of the Chinese Academy of Agricultural Sciences (YBXM15).
文摘Plant organ size is an important agronomic trait that makes a significant contribution to plant yield.Despite its central importance,the genetic and molecular mechanisms underlying organ size control remain to be fully clarified.Here,we report that the trithorax group protein ULTRAPETALA1(ULT1)interacts with the TEOSINTE BRANCHED1/CYCLOIDEA/PCF14/15(TCP14/15)transcription factors by antagonizing the LIN-11,ISL-1,and MEC-3(LIM)peptidase DA1,thereby regulating organ size in Arabidopsis.Loss of ULT1 function significantly increases rosette leaf,petal,silique,and seed size,whereas overexpression of ULT1 results in reduced organ size.ULT1 associates with TCP14 and TCP15 to co-regulate cell size by affecting cellular endoreduplication.Transcriptome analysis revealed that ULT1 and TCP14/15 regulate common target genes involved in endoreduplication and leaf development.ULT1 can be recruited by TCP14/15 to promote lysine 4 of histone H3 trimethylation at target genes,activating their expression to determinefinal cell size.Furthermore,we found that ULT1 influences the interaction of DA1 and TCP14/15 and antagonizes the effect of DA1 on TCP14/15 degradation.Collectively,ourfindings reveal a novel epigenetic mechanism underlying the regulation of organ size in Arabidopsis.
基金This Work Was Supported by the National Natural Sciences Foundation of China (No.30270644, 3021010392)the Natural Sciences Foundation of Huan Province of China (No.02JJY4026)the Foundation of the Department of Education in Hunan Province of China(No.02B017).
