目的明确四川江油地区栽培乌头霜霉病病原菌乌头霜霉Peronospora aconiti rDNA-ITS和28 S rDNA D1/D2区序列,为病害诊断和防治提供理论基础。方法从病株收集病原菌分生孢子及菌丝,提取DNA,扩增rDNA-ITS和28 S rDNA D1/D2片段序列,进行...目的明确四川江油地区栽培乌头霜霉病病原菌乌头霜霉Peronospora aconiti rDNA-ITS和28 S rDNA D1/D2区序列,为病害诊断和防治提供理论基础。方法从病株收集病原菌分生孢子及菌丝,提取DNA,扩增rDNA-ITS和28 S rDNA D1/D2片段序列,进行测序分析,并构建邻接(neighbor-joining,NJ)发育树分析病原菌种类。结果检测出的病原菌rDNA-ITS序列与NCBI数据库中霜霉属P.pulveracea、P.aparines相似度为94%,28 S rDNA D1/D2区序列与霜霉属P.pulveracea、P.ficariae、P.bulbocapni相似度达97%。结论分子rDNA-ITS和28 S rDNA D1/D2区序列鉴定的结论和形态学鉴定的结论一致,乌头霜霉病病原菌为霜霉科霜霉属乌头霜霉Peronospora aconiti Yu,其rDNA-ITS和28 S rDNA D1/D2区序列可用于该病原物的鉴定。展开更多
Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damag...Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damage of pheophytin a (pheo a) in the complex was observed for the first time. The content of pheo a decreased about 47% by illumination, suggesting only one of the two pheo a molecules in the PSⅡ reaction center complex was damaged. No damage of β carotene was found.展开更多
Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The res...Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.展开更多
To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit （LSU） of ribosomal DNA （rDNA） of 25 tested strains for identifica...To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit （LSU） of ribosomal DNA （rDNA） of 25 tested strains for identification and those of ribosomal intergenic space 1 （IGS1） region of 11 strains for subgrouping were detected. The identifications of tested strains were changed except 6 strains. According to the alignment of the IGS1 region, 6 T. asahii isolates tested fell into 4 groups and 5 T. faecale isolates into 3 groups. Polymorphism of 2 T. japonicum isolates was found in 10 positions. With the alignments obtained in this research compared with the relative GenBank entries, it was found that T. asahii, T. faecale and T. japonicum species were divided into 7, 3 and 2 subtypes respectively. Morphological and biophysical methods are not sufficient for Trichosporon spp. identification. Sequencing becomes necessary for Trichosporon diagnosis. There is obvious diversity within a species.展开更多
文摘目的明确四川江油地区栽培乌头霜霉病病原菌乌头霜霉Peronospora aconiti rDNA-ITS和28 S rDNA D1/D2区序列,为病害诊断和防治提供理论基础。方法从病株收集病原菌分生孢子及菌丝,提取DNA,扩增rDNA-ITS和28 S rDNA D1/D2片段序列,进行测序分析,并构建邻接(neighbor-joining,NJ)发育树分析病原菌种类。结果检测出的病原菌rDNA-ITS序列与NCBI数据库中霜霉属P.pulveracea、P.aparines相似度为94%,28 S rDNA D1/D2区序列与霜霉属P.pulveracea、P.ficariae、P.bulbocapni相似度达97%。结论分子rDNA-ITS和28 S rDNA D1/D2区序列鉴定的结论和形态学鉴定的结论一致,乌头霜霉病病原菌为霜霉科霜霉属乌头霜霉Peronospora aconiti Yu,其rDNA-ITS和28 S rDNA D1/D2区序列可用于该病原物的鉴定。
文摘Photodamage of some pigments in the isolated photosystem Ⅱ (PS Ⅱ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by means of high performance liquid chromatography. The light induced damage of pheophytin a (pheo a) in the complex was observed for the first time. The content of pheo a decreased about 47% by illumination, suggesting only one of the two pheo a molecules in the PSⅡ reaction center complex was damaged. No damage of β carotene was found.
文摘Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.
基金supported by the National Bio-Resource Project "Pathogenic microbes" from the Ministry of Education,Culture,Sports,Science and Technology of the Japanese Governments
文摘To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit （LSU） of ribosomal DNA （rDNA） of 25 tested strains for identification and those of ribosomal intergenic space 1 （IGS1） region of 11 strains for subgrouping were detected. The identifications of tested strains were changed except 6 strains. According to the alignment of the IGS1 region, 6 T. asahii isolates tested fell into 4 groups and 5 T. faecale isolates into 3 groups. Polymorphism of 2 T. japonicum isolates was found in 10 positions. With the alignments obtained in this research compared with the relative GenBank entries, it was found that T. asahii, T. faecale and T. japonicum species were divided into 7, 3 and 2 subtypes respectively. Morphological and biophysical methods are not sufficient for Trichosporon spp. identification. Sequencing becomes necessary for Trichosporon diagnosis. There is obvious diversity within a species.
