[Objectives]This study was conducted to investigate the effects of soil selenium(Se)content and exogenous Se supplementation on crop Se levels and provide a theoretical basis for the production of Se-enriched agricult...[Objectives]This study was conducted to investigate the effects of soil selenium(Se)content and exogenous Se supplementation on crop Se levels and provide a theoretical basis for the production of Se-enriched agricultural products.[Methods]Thirty three representative townships in Xiayi County,Ningling County,and Yongcheng City were selected.Soil Se content and its impact on wheat Se level were measured.The effects of exogenous Se supplementation on the Se content of crops including soybean,wheat,peanut,corn,sweet potato,grape,crisp pear and apple were also investigated.[Results]Showed that the soil Se content in 18 townships of Xiayi County ranged from 0.08 to 0.34 mg/kg,and Se-adequate areas,Se-deficient areas and severely Se-deficient areas accounted for 27.78%,66.67%,and 0.055%of the total,respectively.In Ningling County,the soil Se content in 11 townships ranged from 0.13 to 0.23 mg/kg,and among them,Se-adequate areas and Se-deficient areas comprised 18.18%and 81.82%,respectively.In Yongcheng City,the soil Se content in 4 townships ranged from 0.16 to 0.23 mg/kg,and Se-adequate areas and Se-deficient areas constituted 75.00%and 25.00%,respectively.The soil Se content in 33 townships across Xiayi County,Ningling County,and Yongcheng City ranged from 0.07 to 0.34 mg/kg.However,the Se levels in wheat from all 33 townships fail to meet the standard for Se-enriched agricultural products.It indicates that relying solely on soil Se supply is insufficient for crops to achieve the Se level required for Se-enriched agricultural products.Exogenous Se supplementation could effectively increase the Se content in soybean,wheat,peanut,corn,sweet potato,grape,crisp pear,and apple.Among these,soybean,wheat and peanut showed the highest Se levels after supplementation,all exceeding 0.10 mg/kg and meeting the industry standard for Se-enriched agricultural products.[Conclusions]The results of this study indicates that exogenous Se supplementation can serve as a key measure for producing Se-enriched agricultural products.展开更多
Background:Investigators from low-,middle-,and high-income countries representing 6 continents contributed to the development of the Global Adolescent and Child Physical Activity Questionnaire(GAC-PAQ).The GAC-PAQ is ...Background:Investigators from low-,middle-,and high-income countries representing 6 continents contributed to the development of the Global Adolescent and Child Physical Activity Questionnaire(GAC-PAQ).The GAC-PAQ is designed to assess physical activity(PA)across all key domains(i.e.,school,chores,work/volunteering,transport,free time,outdoor time).It aimed to address multiple gaps in global PA surveillance(e.g.,omission of important PA domains,insufficient cultural adaptation,underrepresentation of rural areas in questionnaire validation studies).The purpose of this study was to assess the content validity of the GAC-PAQ among PA experts,8-to 17-year-olds,and one of their parents/guardians,and to discuss changes made to the questionnaire based on participants'feedback.Methods:Sixty-two experts in PA measurement and/or surveillance from 24 countries completed an online survey that included both closed-and open-ended questions about the content validity of the GAC-PAQ.The proportion of experts who agreed or strongly agreed with the items was calculated.Child-parent/guardian dyads from 15 countries(n=250;10-40 per country)participated in a structured cognitive interview to assess the clarity of the questions and response options,and they were encouraged to provide suggestions to improve clarity and facilitate completion of the questionnaire.Participating countries are:Aotearoa New Zealand,Brazil,Canada,China,Colombia,Czech Republic,India,Malawi,Mexico,Nepal,Nigeria,Spain,Sweden,Thailand,and the United Arab Emirates.Interviews were conducted in 13 different languages and structured by PA domain.Generic images were included to help participants in answering questions about PA intensity.Results:Expert agreement with the items for each domain exceeded 75%,and their qualitative feedback was used to revise the questionnaire before cognitive interviews.In general,participants found the questionnaire to be comprehensive.Adolescents(12-17 years)found it easier than children(8-11 years)to answer the questions.Several children struggled to answer questions about the duration and intensity of activities and/or concepts related to travel modes,active trips,and organized activities.Many parents/guardians were unsure about the frequency,duration,and intensity of their children's or adolescents'PA at school and/or recommended using more culturally relevant and appropriate images.Some participants misunderstood the concept of activities that“make you stronger”(intended to assess resistance activities)and/or struggled to differentiate between work,volunteering,and chores.Conclusion:Participants'feedback was used to develop a revised,simplified,and culturally adapted GAC-PAQ,which will be pilot-tested in all15 countries in an App that will include country-specific images and narration in local languages.Further research is needed to assess the reliability and validity of the revised GAC-PAQ.展开更多
Sichuan sausages with moisture contents of 40%,45%,50%,and 60% were stored at-18℃for durations of 0,2,4,6,and 8 weeks to evaluate the effect of moisture content on the quality attributes of Sichuan sausages during fr...Sichuan sausages with moisture contents of 40%,45%,50%,and 60% were stored at-18℃for durations of 0,2,4,6,and 8 weeks to evaluate the effect of moisture content on the quality attributes of Sichuan sausages during frozen storage.Product indicators including pH,colour,thiobarbituric acid reactive substances(TBARS),total volatile basic nitrogen(TVB-N),texture,electronic nose(E-nose)response,and water-holding capacity(thawing and cooking losses)were determined.The results indicated that as storage time increased,water retention in Sichuan sausages with different moisture contents decreased,while the degree of protein and lipid oxidation increased.This led to an increase in pH value,a colour shift from red-bright to grey-brown,and a deterioration in palatability.Among the samples,sausages with 50% moisture content exhibited the lowest thawing and cooking losses,indicating superior water-holding capacity.After 8 weeks of storage,TBARS and TVB-N values for the 50% moisture group were 19.5%and 2.5%lower,respectively,than those of the 40%and 45%moisture groups,indicating a reduced degree of oxidation.Furthermore,Sichuan sausage with 50%moisture content demonstrated an appropriate pH and colour difference,along with excellent texture and flavour,as evidenced by its higher toughness and satisfactory hardness.In conclusion,Sichuan sausage with 50% moisture content demonstrated the highest overall quality under frozen storage conditions.展开更多
This paper provides a systematic review of the histochemical localization,content characteristics,and influencing factors of saponins in Pseudostellaria heterophylla based on an extensive literature survey.It provides...This paper provides a systematic review of the histochemical localization,content characteristics,and influencing factors of saponins in Pseudostellaria heterophylla based on an extensive literature survey.It provides an in-depth analysis and summary of the effects of biological factors,environmental conditions,agronomic practices,processing methods,and continuous cropping obstacles on the synthesis of P.heterophylla saponins,as well as their underlying mechanisms.Based on identified gaps in the current literature,future research directions and prospects are proposed.The findings of this review offer valuable insights for advancing the understanding of the saponin biosynthesis mechanisms in P.heterophylla and for enhancing its quality.展开更多
Rock-ice avalanches in cold high-mountain regions pose severe hazards due to their high mobility,yet the quantitative controls of particle-size ratio and ice content remain insufficiently constrained.This study invest...Rock-ice avalanches in cold high-mountain regions pose severe hazards due to their high mobility,yet the quantitative controls of particle-size ratio and ice content remain insufficiently constrained.This study investigates their coupled effects using inclinedflume experiments and Discrete Element Method(DEM)simulations,covering three gravel sizes(2-5 mm,5-7 mm,7-10 mm)and four ice-content levels(0%,20%,40%,60%).Run-out distance,velocity,energy components,flow regime(Savage number),and segregation indexαwere quantified.Increasing ice content significantly enhances mobility,but with diminishing marginal effectiveness.From 0%to 40%ice content,run-out distance increases by 41%-86%,whereas the additional increase from 40%to 60%contributes only 12%-23%.Particle-size ratio strongly governs segregation intensity.Fine-gravel groups reach segregation indices ofα=0.92-0.98,indicating nearly complete upward migration of ice,whereas medium-gravel and coarse-gravel groups exhibit much weaker segregation,stabilizing atα=0.68-0.74 and 0.60-0.69.Savage number analyses reveal marked flow-regime transitions.At 0%ice content,Savage numbers reach 1.0-1.5,indicating a collisional regime.Increasing ice content suppresses collisionality,with Savage numbers decreasing to 0.03-0.07 at 60%ice content,consistent with dense-regime flow.DEM energy analyses confirm this regime shift:for finegravel mixtures,collision energy decreases by 14%,while sliding-friction energy increases by 33%as ice content increases from 0%to 60%,reflecting enhanced overburden effects imposed by upward-segregated ice layers.Medium and coarse mixtures exhibit weaker or opposite energy-shift patterns,demonstrating strong size dependence.Mechanistically,large particle-size contrasts promote strong segregation and form dense basal rock layers that increase basal friction and reduce mobility.When particle sizes are similar or ice content is high,segregation remains limited,allowing ice to mix into the basal layer,thereby reducing basal friction and enhancing mobility.This research quantitatively demonstrates how composition controls particle spatial distribution,flow regime,and energy dissipation,offering new mechanistic insights into the propagation and deposition behaviors of rock-ice avalanches and improving hazard assessment in vulnerable high-mountain regions.展开更多
The Yellow Sea and Bohai Sea are among the global shelf seas susceptible to typhoons every year.Using observations and high-resolution numerical simulations,the current study investigates the dramatic changes in tempe...The Yellow Sea and Bohai Sea are among the global shelf seas susceptible to typhoons every year.Using observations and high-resolution numerical simulations,the current study investigates the dramatic changes in temperature and ocean heat content(OHC)of the Yellow Sea and Bohai Sea caused by Super Typhoon Maysak in early September 2020,which is representative of northward/northeastward-bypassing typhoons with centers just to the east of the study area.Temperature shows spatially coherent cooling in the upper mixed layer but warming in the subsurface layer in the majority of the offshore waters,due to wind-enhanced vertical mixing.In lower layers from the thermocline to sea bottom,temperature experiences significant warming in northeastern coastal waters of the Shandong Peninsula and in regions just off the Subei Shoal,but significant cooling in western coastal waters of the Korean Peninsula and southern coastal waters of the Shandong Peninsula.Significant temperature warming/cooling in lower layers is caused by coastal downwelling/upwelling.The total OHC of the study area decreases rapidly during Typhoon Maysak(2020)’s passage,which is generated comparably by latent heat loss at the sea surface and southward heat advection out of the study area at the southern boundary.Reduced shortwave radiation contributes positively but secondarily to the decreasing OHC during the first day.A numerical experiment suggests that Typhoon Maysak(2020)-induced OHC decline could have greatly affected the regional climate evolution in the following seasons.More studies are needed to fully understand the impacts of typhoons on regional climate changes in shelf seas at different time scales.展开更多
Slope units are divided according to the real topography and have clear geological characteristics,making them ideal units for evaluating the susceptibility to geological disasters.Based on the results of automaticall...Slope units are divided according to the real topography and have clear geological characteristics,making them ideal units for evaluating the susceptibility to geological disasters.Based on the results of automatically and manually corrected hydrological slope unit division,the Longhua District,Shenzhen City,Guangdong Province,was selected as the study area.A total of 15 influencing factors,namely Fluctuation,slope,slope aspect,curvature,topographic witness index(TWI),stream power index(SPI),topographic roughness index(TRI),annual average rainfall,distance to water system,engineering rock group,distance to fault,land use,normalized difference vegetation index(NDVI),nighttime light,and distance to road,were selected as evaluation indicators.The information volume model(IV)and random points were used to select non-geological disaster units,and then the random forest model(RF)was used to evaluate the susceptibility to geological disasters.The automatic slope unit and the hydrological slope unit were compared and analyzed in the random forest and information volume random forest models.The results show that the area under the curve(AUC)values of the automatic slope unit evaluation results are 0.931 for the IV-RF model and 0.716 for the RF model,which are 0.6%(IV-RF model)and 1.9%(RF model)higher than those for the hydrological slope unit.Based on a comparison of the evaluation methods based on the two types of slope units,the hydrological slope unit evaluation method based on manual correction is highly subjective,is complicated to operate,and has a low evaluation accuracy,whereas the evaluation method based on automatic slope unit division is efficient and accurate,is suitable for large-scale efficient geological disaster evaluation,and can better deal with the problem of geological disaster susceptibility evaluation.展开更多
Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify...Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify TSEMF in response to environmental changes.However,there has been limited exploration of multitrait combinations for predicting TS-EMF across seasons and of trait thresholds that enhance TS-EMF.Here,for 10 dominant tree species in urban forests of Northeast China,14 traits were measured and four aboveground and three belowground ecological functions assessed in three seasons.Ecological functions and TS-EMF differed significantly throughout the seasons(P<0.05).Synergistic relationships were found between carbon sequestration and oxygen release,between cooling and humidification,and between organic carbon accumulation and nutrient cycling.Notably,aboveground multifunctionality played a leading role in TS-EMF.With seasonal changes,resource allocation shifted toward traits related to resource acquisition rather than conservation to maintain TS-EMF.The combination of traits that predicted TS-EMF varied by type,accounting for up to 66.45%of the variation.TS-EMF was primarily driven by leaf structure in spring and by nutrient accumulation in autumn.Leaf carbon content(LCC)consistently served as a stabilizing factor for predicting TS-EMF across seasons.At 36.5-36.8 mg g^(-1),LCC had its optimal effect on TS-EMF.Other traits in combination that positively influence total TS-EMF include leaf nitrogen content(3.43-3.45 mg g^(-1)),leaf phosphorus content(0.80-0.83 mg g^(-1)),and leaf area(65.86-68.43 cm^(2)).Within these specified trait thresholds,Morus alba and Quercus mongolica were identified as key species.These findings suggest that the trade-off between various ecological functions can be managed by altering plant traits across seasons.This approach could provide a theoretical foundation for enhancing the TS-EMF of urban forests through trait-based management,offering practical guidance for selecting tree species.展开更多
Extreme climate events(e.g.,heatwaves and droughts)are becoming increasingly frequent due to global climate change,which inevitably affects tree growth and various other ecological processes.While the impacts of droug...Extreme climate events(e.g.,heatwaves and droughts)are becoming increasingly frequent due to global climate change,which inevitably affects tree growth and various other ecological processes.While the impacts of droughts on these processes have been widely evaluated,the effects of heatwaves on tree growth and soil water content(SWC)remain poorly understood,particularly those related to thinning treatment.In this study,we evaluated the impacts of the 2021 Pacific Northwest Heatwave and thinning on forest growth and SWC,as well as assessed how thinning might mitigate the heatwave's impacts in lodgepole pine forests in British Columbia,Canada.We measured meteorological data(air temperature,rainfall,solar radiation(SR),relative humidity(RH),and wind speed(W_(s)),sap flow,SWC,soil temperature(T_(s)),and tree diameters at the breast height(DBH)during the growing season(June–September)in the control(27,000 stems·ha^(-1)),lightly thinned(4,500 stems·ha^(-1)),and heavily thinned(1,100 stems·ha^(-1))experimental plots from 2018 to 2024.We found that thinning persistently and significantly(p<0.05)increased individual tree growth,with the most pronounced effects in the heavily thinned stands.The 2021 Pacific Northwest Heatwave led to an exceptionally hot growing season,significantly(p<0.05)reducing forest growth and SWC across all plots.Forest growth recovered in 2022 in the thinned plots but remained suppressed in the unthinned plots,suggesting that thinning effectively mitigated the impact of the heatwave on forest growth,while the heatwave's impacts were persistent in the unthinned plots.Our study highlights that thinning is a practical management strategy for improving tree growth and supporting climate change adaptation to extreme climate events.展开更多
By investigating the evolution of shale gas generation,storage,adjustment and accumulation under different structural settings in superimposed basins,this study elucidates the differential accumulation mechanisms of s...By investigating the evolution of shale gas generation,storage,adjustment and accumulation under different structural settings in superimposed basins,this study elucidates the differential accumulation mechanisms of shale gas.An improved evaluation method of shale gas content evolution in superimposed basins is proposed.This method incorporates the coupling effect of key geological factors such as temperature,pressure,organic matter abundance,maturity,and pore characteristics on the content and occurrence state of shale gas,as well as the configuration relationship between shale gas generation and storage throughout geological history.Using this approach,the gas evolution histories of the Longmaxi Formation shales in wells N201 and PY1 are reconstructed under varying geological conditions.The Longmaxi Formation shales in these wells are dominated by typeⅠkerogen,with original total organic carbon(TOC_(o))contents of 6.20 wt% and 4.92 wt%,respectively,indicating differences in the initial material basis for gas generation.At the maximum burial depth of approximately 5000 m,the Longmaxi Formation shale in well N201 exhibits a formation pressure coefficient of 2.05,an organic matter maturity of 2.2%,and organic pores accounting for 68%of the total porosity.The gas generation quantity(Q_(g))reaches 19.24 m^(3)/t,while the gas storage capacity(Q_(s))is 4.30 m^(3)/t.The actual total gas content(Q_(a)),constrained by Q_(s),is 4.30 m^(3)/t,with free gas comprising 94%.Following relatively moderate tectonic uplift,the Q_(a) in well N201 decreases to 4.03 m^(3)/t,with free gas accounting for 63%.In contrast,the Longmaxi Formation shale in well PY1 reached a maximum burial depth of 6300 m,associated with a formation pressure coefficient of 1.62,organic matter maturity of 2.5%,and organic pore proportion of 67%.Here,Q_(g) is 16.87 m^(3)/t,and both Q_(s) and Q_(a) are 3.65 m^(3)/t,with free gas accounting for 98%.After intense tectonic uplift,Q_(a) declines to 2.72 m^(3)/t,and the proportion of free gas drops to51%.Finally,a four-stage differential accumulation model of shale gas is established:Slow gas generation and only adsorbed gas occur in stageⅠ,which is primarily controlled by TOC content;both adsorbed gas and free gas present in stageⅡ,with free gas becoming dominant;rapid gas generation and free gas predominance are controlled by temperature and porosity in stageⅢ;and gas adjustment and accumulation are primarily controlled by temperature and pressure in stageⅣ.展开更多
[Objectives]To determine the content of Zhuang medicine Sauropus spatulifolius Beille from Guangxi.[Methods]The amino acid content of S.spatulifolius Beille was determined by ultraviolet spectrophotometry(UVs).The con...[Objectives]To determine the content of Zhuang medicine Sauropus spatulifolius Beille from Guangxi.[Methods]The amino acid content of S.spatulifolius Beille was determined by ultraviolet spectrophotometry(UVs).The content of kaempferol-3-O-gentiobioside in S.spatulifolius Beille was determined by liquid chromatography-mass spectrometry(LC-MS).Pesticide residues in S.spatulifolius Beille were detected by gas chromatography-mass spectrometry(GC-MS).Heavy metal elements arsenic(As),cadmium(Cd),and lead(Pb)in S.spatulifolius Beille were detected by inductively coupled plasma mass spectrometry(ICP-MS).[Results]The amino acid content in S.spatulifolius Beille was 3.233 mg/g,with a relative standard deviation(RSD)of 0.36%.The content of kaempferol-3-O-gentiobioside was 1.15μg/mL.No pesticide residues or heavy metals were detected in the S.spatulifolius Beille medicinal material.[Conclusions]This study improves the quality control system for S.spatulifolius Beille and provides a reference basis for the quality standard control of Zhuang medicine S.spatulifolius Beille from Guangxi.展开更多
Objective To investigate the mechanisms of electroacupuncture （EA） at ＂Baihui＂ （百会 GV 20） and ＂Shuigou＂ （水沟 GV 26） for treating cerebral infarction. Methods Forty healthy SD rats were randomly divided in...Objective To investigate the mechanisms of electroacupuncture （EA） at ＂Baihui＂ （百会 GV 20） and ＂Shuigou＂ （水沟 GV 26） for treating cerebral infarction. Methods Forty healthy SD rats were randomly divided into model group （n = 10）, EA group （n = 10）, normal group （n = 10）, and sham operation group （n = 10）. The model of acute cerebral infarction was established by blocking the middle cerebral artery with an intraluminal thread. EA was applied at ＂Baihui＂ （百会 GV 20） and ＂Shuigou＂ （水沟GV 26） in the rats of EA group after the models were developed. The contents of D-dimer （D-D） and fibrinogen （Fib） were measured in each group. Results The contents of D-D and Fib increased signifi- cantly in model group as compared with normal and sham operation groups, respectively （P〈0.01）, and they decreased significantly in EA group as compared with model group （P〈0.01）. Conclusion EA can ob-viously decrease the contents of D-D and Fib, improve abnormal fibrinolysis, lower blood viscosity and ameliorate cerebral blood circulation in the rats with acute cerebral infarction.展开更多
BACKGROUND Type 2 diabetes mellitus(T2DM)often leads to vascular complications,such as albuminuria.The role of insulin autoantibodies(IAA)and their interaction with D-dimer in this context remains unclear.AIM To inves...BACKGROUND Type 2 diabetes mellitus(T2DM)often leads to vascular complications,such as albuminuria.The role of insulin autoantibodies(IAA)and their interaction with D-dimer in this context remains unclear.AIM To investigate the characteristics of IAA and its effect on albuminuria in T2DM patients.METHODS We retrospectively analyzed clinical data from 115 T2DM patients with positive IAA induced by exogenous insulin,and 115 age-and sex-matched IAA-negative T2DM patients as controls.Propensity scores were calculated using multivariate logistic regression.Key variables were selected using the least absolute shrinkage and selection operator(LASSO)algorithm.We constructed a prediction model and analyzed the association between IAA and albuminuria based on demographic and laboratory parameters.RESULTS The IAA-positive group had significantly higher D-dimer levels[0.30(0.19-0.55)mg/L vs 0.21(0.19-0.33)mg/L,P=0.008]and plasma insulin levels[39.1(12.0-102.7)μU/mL vs 9.8(5.5-17.6)μU/mL,P<0.001]compared to the IAA-negative group.Increases in the insulin dose per weight ratio,diabetes duration,and urinary albumin-to-creatinine ratio(UACR)were observed but did not reach statistical significance.The LASSO model identified plasma insulin and D-dimer as key factors with larger coefficients.D-dimer was significantly associated with UACR in the total and IAA-positive groups but not in the IAA-negative group.The odds ratio for D-dimer elevation(>0.5 g/L)was 2.88(95%confidence interval:1.17-7.07)in the IAA-positive group(P interaction<0.05).CONCLUSION D-dimer elevation is an independent risk factor for abnormal albuminuria and interacts with IAA in the development of abnormal albuminuria in T2DM patients.展开更多
Salt stress significantly inhibits crop growth and development,and mitigating this can enhance salt tolerance in various crops.Previous studies have shown that regulating saccharide biosynthesis is a key aspect of pla...Salt stress significantly inhibits crop growth and development,and mitigating this can enhance salt tolerance in various crops.Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance;however,the underlying molecular mechanisms remain largely unexplored.In this study,we demonstrate that overexpression of a salt-inducible galactinol synthase gene,ZmGolS1,alleviates salt-induced growth inhibition,likely by promoting raffinose synthesis.Additionally,we show that natural variation in ZmGolS1 transcript levels contributes to the diversity of raffinose content and salt tolerance in maize.We further reveal that ZmRR18,a type-B response regulator transcription factor,binds to the AATC element in the promoter of ZmGolS1,with this binding increases the transcript levels of ZmGolS1 under salt conditions.Moreover,a single nucleotide polymorphism(termed SNP-302T)within the ZmGolS1 promoter significantly reduces its binding affinity for ZmRR18,resulting in decreased ZmGolS1 expression and diminished raffinose content,ultimately leading to a salt-hypersensitive phenotype.Collectively,our findings reveal the molecular mechanisms by which the ZmRR18-ZmGolS1 module enhances raffinose biosynthesis,thereby promoting maize growth under salt conditions.This research provides important insights into salt tolerance mechanisms associated with saccharide biosynthesis and identifies valuable genetic loci for breeding salt-tolerant maize varieties.展开更多
Gas content serves as a critical indicator for assessing the resource potential of deep coal mines and forecasting coal mine gas outburst risks.However,existing sampling technologies face challenges in maintaining the...Gas content serves as a critical indicator for assessing the resource potential of deep coal mines and forecasting coal mine gas outburst risks.However,existing sampling technologies face challenges in maintaining the integrity of gas content within samples and are often constrained by estimation errors inherent in empirical formulas,which results in inaccurate gas content measurements.This study introduces a lightweight,in-situ pressure-and gas-preserved corer designed to collect coal samples under the pressure conditions at the sampling point,effectively preventing gas loss during transfer and significantly improving measurement accuracy.Additionally,a gas migration model for deep coal mines was developed to elucidate gas migration characteristics under pressure-preserved coring conditions.The model offers valuable insights for optimizing coring parameters,demonstrating that both minimizing the coring hole diameter and reducing the pressure difference between the coring-point pressure and the original pore pressure can effectively improve the precision of gas content measurements.Coring tests conducted at an experimental base validated the performance of the corer and its effectiveness in sample collection.Furthermore,successful horizontal coring tests conducted in an underground coal mine roadway demonstrated that the measured gas content using pressure-preserved coring was 34%higher than that obtained through open sampling methods.展开更多
Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confi...Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.展开更多
Sodium-ion batteries(SIBs)employ P2-type layered transition metal oxides as promising cathode materials,primarily due to their abundant natural reserves and environmentally friendly characteristics.However,structural ...Sodium-ion batteries(SIBs)employ P2-type layered transition metal oxides as promising cathode materials,primarily due to their abundant natural reserves and environmentally friendly characteristics.However,structural instability and complex phase transitions during electrochemical cycling pose significant challenges to their practical applications.Employing cation substitution serves as a straightforward yet effective strategy for stabilizing the structure and improving the kinetics of the active material.In this study,we introduce a Ni-rich honeycomb-layered Na_(2+x)Ni_(2)TeO_(6)(NNTO)cathode material with variable sodium content(x=0,0.03,0.05,0.10).Physicochemical characterizations reveal that excess sodium content at the atomic scale modifies the surface and suppresses phase transitions,while preserving the crystal structure.This results in enhanced cyclic performance and improved electrochemical kinetics at room temperature.Furthermore,we investigate the performance of the NNTO cathode material containing 10%excess sodium at a relatively high temperature of 60℃,where it exhibits 71.6%capacity retention compared to 60%for the pristine.Overall,our results confirm that a preconstructed surface layer(induced by excess sodium)effectively safeguards the Ni-based cathode material from surface degradation and phase transitions during the electrochemical processes,thus exhibiting superior capacity retention relative to the pristine NNTO cathode.This study of the correlation between structure and performance can potentially be applied to the commercialization of SIBs.展开更多
The endpoint carbon content in the converter is critical for the quality of steel products,and accurately predicting this parameter is an effective way to reduce alloy consumption and improve smelting efficiency.Howev...The endpoint carbon content in the converter is critical for the quality of steel products,and accurately predicting this parameter is an effective way to reduce alloy consumption and improve smelting efficiency.However,most scholars currently focus on modifying methods to enhance model accuracy,while overlooking the extent to which input parameters influence accuracy.To address this issue,in this study,a prediction model for the endpoint carbon content in the converter was developed using factor analysis(FA)and support vector machine(SVM)optimized by improved particle swarm optimization(IPSO).Analysis of the factors influencing the endpoint carbon content during the converter smelting process led to the identification of 21 input parameters.Subsequently,FA was used to reduce the dimensionality of the data and applied to the prediction model.The results demonstrate that the performance of the FA-IPSO-SVM model surpasses several existing methods,such as twin support vector regression and support vector machine.The model achieves hit rates of 89.59%,96.21%,and 98.74%within error ranges of±0.01%,±0.015%,and±0.02%,respectively.Finally,based on the prediction results obtained by sequentially removing input parameters,the parameters were classified into high influence(5%-7%),medium influence(2%-5%),and low influence(0-2%)categories according to their varying degrees of impact on prediction accuracy.This classi-fication provides a reference for selecting input parameters in future prediction models for endpoint carbon content.展开更多
基金Supported by Shangqiu Science and Technology Project(20240036).
文摘[Objectives]This study was conducted to investigate the effects of soil selenium(Se)content and exogenous Se supplementation on crop Se levels and provide a theoretical basis for the production of Se-enriched agricultural products.[Methods]Thirty three representative townships in Xiayi County,Ningling County,and Yongcheng City were selected.Soil Se content and its impact on wheat Se level were measured.The effects of exogenous Se supplementation on the Se content of crops including soybean,wheat,peanut,corn,sweet potato,grape,crisp pear and apple were also investigated.[Results]Showed that the soil Se content in 18 townships of Xiayi County ranged from 0.08 to 0.34 mg/kg,and Se-adequate areas,Se-deficient areas and severely Se-deficient areas accounted for 27.78%,66.67%,and 0.055%of the total,respectively.In Ningling County,the soil Se content in 11 townships ranged from 0.13 to 0.23 mg/kg,and among them,Se-adequate areas and Se-deficient areas comprised 18.18%and 81.82%,respectively.In Yongcheng City,the soil Se content in 4 townships ranged from 0.16 to 0.23 mg/kg,and Se-adequate areas and Se-deficient areas constituted 75.00%and 25.00%,respectively.The soil Se content in 33 townships across Xiayi County,Ningling County,and Yongcheng City ranged from 0.07 to 0.34 mg/kg.However,the Se levels in wheat from all 33 townships fail to meet the standard for Se-enriched agricultural products.It indicates that relying solely on soil Se supply is insufficient for crops to achieve the Se level required for Se-enriched agricultural products.Exogenous Se supplementation could effectively increase the Se content in soybean,wheat,peanut,corn,sweet potato,grape,crisp pear,and apple.Among these,soybean,wheat and peanut showed the highest Se levels after supplementation,all exceeding 0.10 mg/kg and meeting the industry standard for Se-enriched agricultural products.[Conclusions]The results of this study indicates that exogenous Se supplementation can serve as a key measure for producing Se-enriched agricultural products.
基金supported by a Project Grant(Grant No.PJT183705)an Early Career Investigator Prize(Grant No.ECP 184184)from the Canadian Institutes of Health Research+7 种基金a Prentice Institute Research Affiliate Fund Grant from the Prentice Institute for Global Population and Economy(Grant No.G00004116)a Te Herenga Waka Victoria University of Wellington Division of Science Health Engineering Architecture and Design Innovation Faculty Strategic Research Grant(Grant No.FSRG-SHEADI-10724)The Thailand Physical Activity Knowledge Development Centre(TPAK)/Thai Health Promotion Foundation provided funding for the cognitive interviews and pilot study in Thailand(Grant No.66-P1-0473)The University Pablo de Olavide provided a scholarship for 2 undergraduate students working on the project(codes PPI2207 and PPI2308)In the Czech Republicthe study was supported by Palacky University IGA(Grant No.IGA_FTK_2023_017)supported by the Division of Intramural Research at the National Institute on Minority Health and Health Disparities of the National Institutes of Healthsupported by the Key Project of the National Philosophy and Social Science Foundation of China(23&ZD197)。
文摘Background:Investigators from low-,middle-,and high-income countries representing 6 continents contributed to the development of the Global Adolescent and Child Physical Activity Questionnaire(GAC-PAQ).The GAC-PAQ is designed to assess physical activity(PA)across all key domains(i.e.,school,chores,work/volunteering,transport,free time,outdoor time).It aimed to address multiple gaps in global PA surveillance(e.g.,omission of important PA domains,insufficient cultural adaptation,underrepresentation of rural areas in questionnaire validation studies).The purpose of this study was to assess the content validity of the GAC-PAQ among PA experts,8-to 17-year-olds,and one of their parents/guardians,and to discuss changes made to the questionnaire based on participants'feedback.Methods:Sixty-two experts in PA measurement and/or surveillance from 24 countries completed an online survey that included both closed-and open-ended questions about the content validity of the GAC-PAQ.The proportion of experts who agreed or strongly agreed with the items was calculated.Child-parent/guardian dyads from 15 countries(n=250;10-40 per country)participated in a structured cognitive interview to assess the clarity of the questions and response options,and they were encouraged to provide suggestions to improve clarity and facilitate completion of the questionnaire.Participating countries are:Aotearoa New Zealand,Brazil,Canada,China,Colombia,Czech Republic,India,Malawi,Mexico,Nepal,Nigeria,Spain,Sweden,Thailand,and the United Arab Emirates.Interviews were conducted in 13 different languages and structured by PA domain.Generic images were included to help participants in answering questions about PA intensity.Results:Expert agreement with the items for each domain exceeded 75%,and their qualitative feedback was used to revise the questionnaire before cognitive interviews.In general,participants found the questionnaire to be comprehensive.Adolescents(12-17 years)found it easier than children(8-11 years)to answer the questions.Several children struggled to answer questions about the duration and intensity of activities and/or concepts related to travel modes,active trips,and organized activities.Many parents/guardians were unsure about the frequency,duration,and intensity of their children's or adolescents'PA at school and/or recommended using more culturally relevant and appropriate images.Some participants misunderstood the concept of activities that“make you stronger”(intended to assess resistance activities)and/or struggled to differentiate between work,volunteering,and chores.Conclusion:Participants'feedback was used to develop a revised,simplified,and culturally adapted GAC-PAQ,which will be pilot-tested in all15 countries in an App that will include country-specific images and narration in local languages.Further research is needed to assess the reliability and validity of the revised GAC-PAQ.
基金Supported by National Modern Agricultural Industry Technology System Sichuan Pig Innovation Team(SCCXTD-2026-8)Sichuan Science and Technology Program(2025ZYD0049)"Challenge and Leadership"Project for Key Core Technologies of Sui Ning in Sichuan(2025SNKBZ19).
文摘Sichuan sausages with moisture contents of 40%,45%,50%,and 60% were stored at-18℃for durations of 0,2,4,6,and 8 weeks to evaluate the effect of moisture content on the quality attributes of Sichuan sausages during frozen storage.Product indicators including pH,colour,thiobarbituric acid reactive substances(TBARS),total volatile basic nitrogen(TVB-N),texture,electronic nose(E-nose)response,and water-holding capacity(thawing and cooking losses)were determined.The results indicated that as storage time increased,water retention in Sichuan sausages with different moisture contents decreased,while the degree of protein and lipid oxidation increased.This led to an increase in pH value,a colour shift from red-bright to grey-brown,and a deterioration in palatability.Among the samples,sausages with 50% moisture content exhibited the lowest thawing and cooking losses,indicating superior water-holding capacity.After 8 weeks of storage,TBARS and TVB-N values for the 50% moisture group were 19.5%and 2.5%lower,respectively,than those of the 40%and 45%moisture groups,indicating a reduced degree of oxidation.Furthermore,Sichuan sausage with 50%moisture content demonstrated an appropriate pH and colour difference,along with excellent texture and flavour,as evidenced by its higher toughness and satisfactory hardness.In conclusion,Sichuan sausage with 50% moisture content demonstrated the highest overall quality under frozen storage conditions.
基金Supported by Open Fund Project of the Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian(PP202003).
文摘This paper provides a systematic review of the histochemical localization,content characteristics,and influencing factors of saponins in Pseudostellaria heterophylla based on an extensive literature survey.It provides an in-depth analysis and summary of the effects of biological factors,environmental conditions,agronomic practices,processing methods,and continuous cropping obstacles on the synthesis of P.heterophylla saponins,as well as their underlying mechanisms.Based on identified gaps in the current literature,future research directions and prospects are proposed.The findings of this review offer valuable insights for advancing the understanding of the saponin biosynthesis mechanisms in P.heterophylla and for enhancing its quality.
基金funded by the Natural Science Foundation of China(Grants No 42277127)。
文摘Rock-ice avalanches in cold high-mountain regions pose severe hazards due to their high mobility,yet the quantitative controls of particle-size ratio and ice content remain insufficiently constrained.This study investigates their coupled effects using inclinedflume experiments and Discrete Element Method(DEM)simulations,covering three gravel sizes(2-5 mm,5-7 mm,7-10 mm)and four ice-content levels(0%,20%,40%,60%).Run-out distance,velocity,energy components,flow regime(Savage number),and segregation indexαwere quantified.Increasing ice content significantly enhances mobility,but with diminishing marginal effectiveness.From 0%to 40%ice content,run-out distance increases by 41%-86%,whereas the additional increase from 40%to 60%contributes only 12%-23%.Particle-size ratio strongly governs segregation intensity.Fine-gravel groups reach segregation indices ofα=0.92-0.98,indicating nearly complete upward migration of ice,whereas medium-gravel and coarse-gravel groups exhibit much weaker segregation,stabilizing atα=0.68-0.74 and 0.60-0.69.Savage number analyses reveal marked flow-regime transitions.At 0%ice content,Savage numbers reach 1.0-1.5,indicating a collisional regime.Increasing ice content suppresses collisionality,with Savage numbers decreasing to 0.03-0.07 at 60%ice content,consistent with dense-regime flow.DEM energy analyses confirm this regime shift:for finegravel mixtures,collision energy decreases by 14%,while sliding-friction energy increases by 33%as ice content increases from 0%to 60%,reflecting enhanced overburden effects imposed by upward-segregated ice layers.Medium and coarse mixtures exhibit weaker or opposite energy-shift patterns,demonstrating strong size dependence.Mechanistically,large particle-size contrasts promote strong segregation and form dense basal rock layers that increase basal friction and reduce mobility.When particle sizes are similar or ice content is high,segregation remains limited,allowing ice to mix into the basal layer,thereby reducing basal friction and enhancing mobility.This research quantitatively demonstrates how composition controls particle spatial distribution,flow regime,and energy dissipation,offering new mechanistic insights into the propagation and deposition behaviors of rock-ice avalanches and improving hazard assessment in vulnerable high-mountain regions.
基金supported by the National Key Research and Development Program of China(Grant Nos.2022YFF0801400 and 2021YFF0704002)the Shandong Provincial Natural Science Foundation(Grant No.ZR2024LQX002)the National Science Foundation of China(Grant No.42176016).
文摘The Yellow Sea and Bohai Sea are among the global shelf seas susceptible to typhoons every year.Using observations and high-resolution numerical simulations,the current study investigates the dramatic changes in temperature and ocean heat content(OHC)of the Yellow Sea and Bohai Sea caused by Super Typhoon Maysak in early September 2020,which is representative of northward/northeastward-bypassing typhoons with centers just to the east of the study area.Temperature shows spatially coherent cooling in the upper mixed layer but warming in the subsurface layer in the majority of the offshore waters,due to wind-enhanced vertical mixing.In lower layers from the thermocline to sea bottom,temperature experiences significant warming in northeastern coastal waters of the Shandong Peninsula and in regions just off the Subei Shoal,but significant cooling in western coastal waters of the Korean Peninsula and southern coastal waters of the Shandong Peninsula.Significant temperature warming/cooling in lower layers is caused by coastal downwelling/upwelling.The total OHC of the study area decreases rapidly during Typhoon Maysak(2020)’s passage,which is generated comparably by latent heat loss at the sea surface and southward heat advection out of the study area at the southern boundary.Reduced shortwave radiation contributes positively but secondarily to the decreasing OHC during the first day.A numerical experiment suggests that Typhoon Maysak(2020)-induced OHC decline could have greatly affected the regional climate evolution in the following seasons.More studies are needed to fully understand the impacts of typhoons on regional climate changes in shelf seas at different time scales.
文摘Slope units are divided according to the real topography and have clear geological characteristics,making them ideal units for evaluating the susceptibility to geological disasters.Based on the results of automatically and manually corrected hydrological slope unit division,the Longhua District,Shenzhen City,Guangdong Province,was selected as the study area.A total of 15 influencing factors,namely Fluctuation,slope,slope aspect,curvature,topographic witness index(TWI),stream power index(SPI),topographic roughness index(TRI),annual average rainfall,distance to water system,engineering rock group,distance to fault,land use,normalized difference vegetation index(NDVI),nighttime light,and distance to road,were selected as evaluation indicators.The information volume model(IV)and random points were used to select non-geological disaster units,and then the random forest model(RF)was used to evaluate the susceptibility to geological disasters.The automatic slope unit and the hydrological slope unit were compared and analyzed in the random forest and information volume random forest models.The results show that the area under the curve(AUC)values of the automatic slope unit evaluation results are 0.931 for the IV-RF model and 0.716 for the RF model,which are 0.6%(IV-RF model)and 1.9%(RF model)higher than those for the hydrological slope unit.Based on a comparison of the evaluation methods based on the two types of slope units,the hydrological slope unit evaluation method based on manual correction is highly subjective,is complicated to operate,and has a low evaluation accuracy,whereas the evaluation method based on automatic slope unit division is efficient and accurate,is suitable for large-scale efficient geological disaster evaluation,and can better deal with the problem of geological disaster susceptibility evaluation.
基金supported by the National Natural Science Foundation(32130068,32271634,and 32071597)CAS Key Laboratory of Forest Ecology and Silviculture,Institute of Applied Ecology,Chinese Academy of Sciences(KLFES-2025)。
文摘Urban forests are highly multifunctional and provide numerous ecological functions.Plant functional traits individually or jointly influence the ecological multifunctionality of tree species(TS-EMF)and can also modify TSEMF in response to environmental changes.However,there has been limited exploration of multitrait combinations for predicting TS-EMF across seasons and of trait thresholds that enhance TS-EMF.Here,for 10 dominant tree species in urban forests of Northeast China,14 traits were measured and four aboveground and three belowground ecological functions assessed in three seasons.Ecological functions and TS-EMF differed significantly throughout the seasons(P<0.05).Synergistic relationships were found between carbon sequestration and oxygen release,between cooling and humidification,and between organic carbon accumulation and nutrient cycling.Notably,aboveground multifunctionality played a leading role in TS-EMF.With seasonal changes,resource allocation shifted toward traits related to resource acquisition rather than conservation to maintain TS-EMF.The combination of traits that predicted TS-EMF varied by type,accounting for up to 66.45%of the variation.TS-EMF was primarily driven by leaf structure in spring and by nutrient accumulation in autumn.Leaf carbon content(LCC)consistently served as a stabilizing factor for predicting TS-EMF across seasons.At 36.5-36.8 mg g^(-1),LCC had its optimal effect on TS-EMF.Other traits in combination that positively influence total TS-EMF include leaf nitrogen content(3.43-3.45 mg g^(-1)),leaf phosphorus content(0.80-0.83 mg g^(-1)),and leaf area(65.86-68.43 cm^(2)).Within these specified trait thresholds,Morus alba and Quercus mongolica were identified as key species.These findings suggest that the trade-off between various ecological functions can be managed by altering plant traits across seasons.This approach could provide a theoretical foundation for enhancing the TS-EMF of urban forests through trait-based management,offering practical guidance for selecting tree species.
基金supported by the British Columbia Ministry of Forces through long-term annual contracts with University of British Columbia(Okanagan)(No.RE25SIR242)the Natural Sciences and Engineering Research Council of Canada(NSERC),Discovery Grants Program(No.RGPIN-2021-02628)+1 种基金supported by the China Postdoctoral Science Foundation(No.2024M760387)Heilongjiang Postdoctoral Financial Assistance(No.LBH-Z24062)。
文摘Extreme climate events(e.g.,heatwaves and droughts)are becoming increasingly frequent due to global climate change,which inevitably affects tree growth and various other ecological processes.While the impacts of droughts on these processes have been widely evaluated,the effects of heatwaves on tree growth and soil water content(SWC)remain poorly understood,particularly those related to thinning treatment.In this study,we evaluated the impacts of the 2021 Pacific Northwest Heatwave and thinning on forest growth and SWC,as well as assessed how thinning might mitigate the heatwave's impacts in lodgepole pine forests in British Columbia,Canada.We measured meteorological data(air temperature,rainfall,solar radiation(SR),relative humidity(RH),and wind speed(W_(s)),sap flow,SWC,soil temperature(T_(s)),and tree diameters at the breast height(DBH)during the growing season(June–September)in the control(27,000 stems·ha^(-1)),lightly thinned(4,500 stems·ha^(-1)),and heavily thinned(1,100 stems·ha^(-1))experimental plots from 2018 to 2024.We found that thinning persistently and significantly(p<0.05)increased individual tree growth,with the most pronounced effects in the heavily thinned stands.The 2021 Pacific Northwest Heatwave led to an exceptionally hot growing season,significantly(p<0.05)reducing forest growth and SWC across all plots.Forest growth recovered in 2022 in the thinned plots but remained suppressed in the unthinned plots,suggesting that thinning effectively mitigated the impact of the heatwave on forest growth,while the heatwave's impacts were persistent in the unthinned plots.Our study highlights that thinning is a practical management strategy for improving tree growth and supporting climate change adaptation to extreme climate events.
基金funded by the Sinopec Science and Technology Project(No.P23132)the AAPG Foundation Grants-inAid Program(No.18644937)。
文摘By investigating the evolution of shale gas generation,storage,adjustment and accumulation under different structural settings in superimposed basins,this study elucidates the differential accumulation mechanisms of shale gas.An improved evaluation method of shale gas content evolution in superimposed basins is proposed.This method incorporates the coupling effect of key geological factors such as temperature,pressure,organic matter abundance,maturity,and pore characteristics on the content and occurrence state of shale gas,as well as the configuration relationship between shale gas generation and storage throughout geological history.Using this approach,the gas evolution histories of the Longmaxi Formation shales in wells N201 and PY1 are reconstructed under varying geological conditions.The Longmaxi Formation shales in these wells are dominated by typeⅠkerogen,with original total organic carbon(TOC_(o))contents of 6.20 wt% and 4.92 wt%,respectively,indicating differences in the initial material basis for gas generation.At the maximum burial depth of approximately 5000 m,the Longmaxi Formation shale in well N201 exhibits a formation pressure coefficient of 2.05,an organic matter maturity of 2.2%,and organic pores accounting for 68%of the total porosity.The gas generation quantity(Q_(g))reaches 19.24 m^(3)/t,while the gas storage capacity(Q_(s))is 4.30 m^(3)/t.The actual total gas content(Q_(a)),constrained by Q_(s),is 4.30 m^(3)/t,with free gas comprising 94%.Following relatively moderate tectonic uplift,the Q_(a) in well N201 decreases to 4.03 m^(3)/t,with free gas accounting for 63%.In contrast,the Longmaxi Formation shale in well PY1 reached a maximum burial depth of 6300 m,associated with a formation pressure coefficient of 1.62,organic matter maturity of 2.5%,and organic pore proportion of 67%.Here,Q_(g) is 16.87 m^(3)/t,and both Q_(s) and Q_(a) are 3.65 m^(3)/t,with free gas accounting for 98%.After intense tectonic uplift,Q_(a) declines to 2.72 m^(3)/t,and the proportion of free gas drops to51%.Finally,a four-stage differential accumulation model of shale gas is established:Slow gas generation and only adsorbed gas occur in stageⅠ,which is primarily controlled by TOC content;both adsorbed gas and free gas present in stageⅡ,with free gas becoming dominant;rapid gas generation and free gas predominance are controlled by temperature and porosity in stageⅢ;and gas adjustment and accumulation are primarily controlled by temperature and pressure in stageⅣ.
基金Supported by Guangxi Key Research and Development Program Project(GuiKe AB18221095)Open Fund Project of Guangxi Key Laboratory of Research on Ethnic Medicinal Plants in the Youjiang River Basin(yykf2024-01)+1 种基金High-level Talent Research Project of Youjiang Medical University for Nationalities(1002018079)2023 National-level College Student Innovation and Entrepreneurship Training Program Project(202310599008).
文摘[Objectives]To determine the content of Zhuang medicine Sauropus spatulifolius Beille from Guangxi.[Methods]The amino acid content of S.spatulifolius Beille was determined by ultraviolet spectrophotometry(UVs).The content of kaempferol-3-O-gentiobioside in S.spatulifolius Beille was determined by liquid chromatography-mass spectrometry(LC-MS).Pesticide residues in S.spatulifolius Beille were detected by gas chromatography-mass spectrometry(GC-MS).Heavy metal elements arsenic(As),cadmium(Cd),and lead(Pb)in S.spatulifolius Beille were detected by inductively coupled plasma mass spectrometry(ICP-MS).[Results]The amino acid content in S.spatulifolius Beille was 3.233 mg/g,with a relative standard deviation(RSD)of 0.36%.The content of kaempferol-3-O-gentiobioside was 1.15μg/mL.No pesticide residues or heavy metals were detected in the S.spatulifolius Beille medicinal material.[Conclusions]This study improves the quality control system for S.spatulifolius Beille and provides a reference basis for the quality standard control of Zhuang medicine S.spatulifolius Beille from Guangxi.
基金subsidized by the research project item of the Department of Educationin Hubei Province:No.D200516006
文摘Objective To investigate the mechanisms of electroacupuncture （EA） at ＂Baihui＂ （百会 GV 20） and ＂Shuigou＂ （水沟 GV 26） for treating cerebral infarction. Methods Forty healthy SD rats were randomly divided into model group （n = 10）, EA group （n = 10）, normal group （n = 10）, and sham operation group （n = 10）. The model of acute cerebral infarction was established by blocking the middle cerebral artery with an intraluminal thread. EA was applied at ＂Baihui＂ （百会 GV 20） and ＂Shuigou＂ （水沟GV 26） in the rats of EA group after the models were developed. The contents of D-dimer （D-D） and fibrinogen （Fib） were measured in each group. Results The contents of D-D and Fib increased signifi- cantly in model group as compared with normal and sham operation groups, respectively （P〈0.01）, and they decreased significantly in EA group as compared with model group （P〈0.01）. Conclusion EA can ob-viously decrease the contents of D-D and Fib, improve abnormal fibrinolysis, lower blood viscosity and ameliorate cerebral blood circulation in the rats with acute cerebral infarction.
文摘BACKGROUND Type 2 diabetes mellitus(T2DM)often leads to vascular complications,such as albuminuria.The role of insulin autoantibodies(IAA)and their interaction with D-dimer in this context remains unclear.AIM To investigate the characteristics of IAA and its effect on albuminuria in T2DM patients.METHODS We retrospectively analyzed clinical data from 115 T2DM patients with positive IAA induced by exogenous insulin,and 115 age-and sex-matched IAA-negative T2DM patients as controls.Propensity scores were calculated using multivariate logistic regression.Key variables were selected using the least absolute shrinkage and selection operator(LASSO)algorithm.We constructed a prediction model and analyzed the association between IAA and albuminuria based on demographic and laboratory parameters.RESULTS The IAA-positive group had significantly higher D-dimer levels[0.30(0.19-0.55)mg/L vs 0.21(0.19-0.33)mg/L,P=0.008]and plasma insulin levels[39.1(12.0-102.7)μU/mL vs 9.8(5.5-17.6)μU/mL,P<0.001]compared to the IAA-negative group.Increases in the insulin dose per weight ratio,diabetes duration,and urinary albumin-to-creatinine ratio(UACR)were observed but did not reach statistical significance.The LASSO model identified plasma insulin and D-dimer as key factors with larger coefficients.D-dimer was significantly associated with UACR in the total and IAA-positive groups but not in the IAA-negative group.The odds ratio for D-dimer elevation(>0.5 g/L)was 2.88(95%confidence interval:1.17-7.07)in the IAA-positive group(P interaction<0.05).CONCLUSION D-dimer elevation is an independent risk factor for abnormal albuminuria and interacts with IAA in the development of abnormal albuminuria in T2DM patients.
基金support from the National Key R&D Program of China(2022YFF1001601)the National Science Fund for Distinguished Young Scholars(32325037)+2 种基金the National Natural Science Foundation of China(32201718 and 32401756)the Postdoctoral Innovation Talents Support Program(BX20240420)the China Postdoctoral Science Foundation(2024T171011 and 2023M743817).
文摘Salt stress significantly inhibits crop growth and development,and mitigating this can enhance salt tolerance in various crops.Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance;however,the underlying molecular mechanisms remain largely unexplored.In this study,we demonstrate that overexpression of a salt-inducible galactinol synthase gene,ZmGolS1,alleviates salt-induced growth inhibition,likely by promoting raffinose synthesis.Additionally,we show that natural variation in ZmGolS1 transcript levels contributes to the diversity of raffinose content and salt tolerance in maize.We further reveal that ZmRR18,a type-B response regulator transcription factor,binds to the AATC element in the promoter of ZmGolS1,with this binding increases the transcript levels of ZmGolS1 under salt conditions.Moreover,a single nucleotide polymorphism(termed SNP-302T)within the ZmGolS1 promoter significantly reduces its binding affinity for ZmRR18,resulting in decreased ZmGolS1 expression and diminished raffinose content,ultimately leading to a salt-hypersensitive phenotype.Collectively,our findings reveal the molecular mechanisms by which the ZmRR18-ZmGolS1 module enhances raffinose biosynthesis,thereby promoting maize growth under salt conditions.This research provides important insights into salt tolerance mechanisms associated with saccharide biosynthesis and identifies valuable genetic loci for breeding salt-tolerant maize varieties.
基金supported by the National Natural Science Foundation of China(Nos.51827901,42477191,and 52304033)the Fundamental Research Funds for the Central Universities(No.YJ202449)+1 种基金the Open Research Fund of State Key Laboratory of Geomechanics and Geotechnical Engineering,Institute of Rock and Soil Mechanics,Chinese Academy of Sciences(No.SKLGME022009)the China Postdoctoral Science Foundation(No.2023M742446).
文摘Gas content serves as a critical indicator for assessing the resource potential of deep coal mines and forecasting coal mine gas outburst risks.However,existing sampling technologies face challenges in maintaining the integrity of gas content within samples and are often constrained by estimation errors inherent in empirical formulas,which results in inaccurate gas content measurements.This study introduces a lightweight,in-situ pressure-and gas-preserved corer designed to collect coal samples under the pressure conditions at the sampling point,effectively preventing gas loss during transfer and significantly improving measurement accuracy.Additionally,a gas migration model for deep coal mines was developed to elucidate gas migration characteristics under pressure-preserved coring conditions.The model offers valuable insights for optimizing coring parameters,demonstrating that both minimizing the coring hole diameter and reducing the pressure difference between the coring-point pressure and the original pore pressure can effectively improve the precision of gas content measurements.Coring tests conducted at an experimental base validated the performance of the corer and its effectiveness in sample collection.Furthermore,successful horizontal coring tests conducted in an underground coal mine roadway demonstrated that the measured gas content using pressure-preserved coring was 34%higher than that obtained through open sampling methods.
基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202403)。
文摘Rice is a poor source of folate,an essential micronutrient for the body.Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans.In this study,we confirmed that OsADCS and OsGTPCHI,encoding the initial enzymes necessary for folate synthesis,positively regulate folate accumulation in knockout mutants of both japonica and indica rice backgrounds.The folate content in the low-folate japonica variety was slightly increased by the expression of the indica alleles driven by the endosperm-specific promoter.We further obtained co-expression lines by stacking OsADCS and OsGTPCHI genes;the folate accumulation in brown rice and polished rice reached 5.65μg/g and 2.95μg/g,respectively,representing 37.9-fold and 26.5-fold increases compared with the wild type.Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances,while the expression of other folate synthesis genes was weakly regulated.In addition,we identified Aus rice as a high-folate germplasm carrying superior haplotypes of OsADCS and OsGTPCHI through natural variation.This study provides an alternative and effective complementary strategy for rice biofortification,promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.
基金Korea Institute of Science and Technology,Grant/Award Number:2E33270National Research Foundation of Korea,Grant/Award Number:2020M3H4A3081889。
文摘Sodium-ion batteries(SIBs)employ P2-type layered transition metal oxides as promising cathode materials,primarily due to their abundant natural reserves and environmentally friendly characteristics.However,structural instability and complex phase transitions during electrochemical cycling pose significant challenges to their practical applications.Employing cation substitution serves as a straightforward yet effective strategy for stabilizing the structure and improving the kinetics of the active material.In this study,we introduce a Ni-rich honeycomb-layered Na_(2+x)Ni_(2)TeO_(6)(NNTO)cathode material with variable sodium content(x=0,0.03,0.05,0.10).Physicochemical characterizations reveal that excess sodium content at the atomic scale modifies the surface and suppresses phase transitions,while preserving the crystal structure.This results in enhanced cyclic performance and improved electrochemical kinetics at room temperature.Furthermore,we investigate the performance of the NNTO cathode material containing 10%excess sodium at a relatively high temperature of 60℃,where it exhibits 71.6%capacity retention compared to 60%for the pristine.Overall,our results confirm that a preconstructed surface layer(induced by excess sodium)effectively safeguards the Ni-based cathode material from surface degradation and phase transitions during the electrochemical processes,thus exhibiting superior capacity retention relative to the pristine NNTO cathode.This study of the correlation between structure and performance can potentially be applied to the commercialization of SIBs.
基金financially supported by the National Natural Science Foundation of China(No.52174297).
文摘The endpoint carbon content in the converter is critical for the quality of steel products,and accurately predicting this parameter is an effective way to reduce alloy consumption and improve smelting efficiency.However,most scholars currently focus on modifying methods to enhance model accuracy,while overlooking the extent to which input parameters influence accuracy.To address this issue,in this study,a prediction model for the endpoint carbon content in the converter was developed using factor analysis(FA)and support vector machine(SVM)optimized by improved particle swarm optimization(IPSO).Analysis of the factors influencing the endpoint carbon content during the converter smelting process led to the identification of 21 input parameters.Subsequently,FA was used to reduce the dimensionality of the data and applied to the prediction model.The results demonstrate that the performance of the FA-IPSO-SVM model surpasses several existing methods,such as twin support vector regression and support vector machine.The model achieves hit rates of 89.59%,96.21%,and 98.74%within error ranges of±0.01%,±0.015%,and±0.02%,respectively.Finally,based on the prediction results obtained by sequentially removing input parameters,the parameters were classified into high influence(5%-7%),medium influence(2%-5%),and low influence(0-2%)categories according to their varying degrees of impact on prediction accuracy.This classi-fication provides a reference for selecting input parameters in future prediction models for endpoint carbon content.
