In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill...In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% IBA compared to control （0% IBA）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.展开更多
Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid...Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid （NAA） @ 2000, 4000 （ppm） in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate （50%） was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control （talc powder） failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation ofA. indica under Kashmir conditions.展开更多
Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000...Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000, 4000(ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar.The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments.The highest rooting rate(50%) was recorded in the cuttings with the application of IBA @ 4000 ppm.The cuttings treated with IBA @ 2000 ppm had 25% rooting rate.All other treatments along with control(talc powder) failed to induce rooting.It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A.indica under Kashmir conditions.展开更多
Techniques have been developed for the vegetative propagation of Tetraena mongolica,a rare and endangered species endemic to the western Ordos Plateau in the northcentral portion of China.The purpose of the study was ...Techniques have been developed for the vegetative propagation of Tetraena mongolica,a rare and endangered species endemic to the western Ordos Plateau in the northcentral portion of China.The purpose of the study was to investigate the rooting characteris-tics of the plant species from the aspects of length and ages of cuttings,external hormone concentration,leaf retention,cutting orientations,and cutting positions.The results obtained from the experiments indicated that the rooting ratio of 2-year or 3-year old cuttings was larger than more than of 4-year old cuttings.The influence of external hormone concentration on rooting was ob-vious;the rooting ratio of cuttings was increased by external hormone treatment,especially when treated by ABT1(ABT No.1 Rooting Powder) 50 mg/L for 3 hours,in which case,the rooting ratio reached 91 percent.Leaf retention influenced rooting sig-nificantly;the rooting rate of cuttings became greater as the leaf retention increased.There was no significant difference in rooting capability between south and north orientations of the ortets.The rooting ratio of the cuttings obtained from the distal portions of shoots was significantly larger than those obtained from the middle and the basal portions of the shoots.On day three after treat-ment,calluses started appearing at the incisions of some cuttings dipped in 500 mg/L IAA(indole acetic acid).Most cuttings were healed or swelled,and 6 percent of cuttings rooted on day 6 after treatment.Higher rooting period occurred 7-10 days after the experiment was set up.展开更多
The present study investigated the rooting ability and growth performance of juvenile single-node leafy stem cuttings of Holarrhena pubescens, collected from two mature mother trees preserved in the hill forests of Ch...The present study investigated the rooting ability and growth performance of juvenile single-node leafy stem cuttings of Holarrhena pubescens, collected from two mature mother trees preserved in the hill forests of Chittagong District, Bangladesh. The rooting ability of cuttings was studied with 0, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. The better rooting response was observed with 0.1% IBA, but was insignificant compared to other treatments. The mean number of roots and length of the longest root of cuttings in different treatments showed no significant difference. After transfer to the polythene bags from a nonmist propagator, rooted cuttings treated with 0.4% IBA demonstrated the highest （50.00± 0.78%） survival capacity. Regarding initial growth of established rooted cuttings （stecklings） over a 90 d period, the increment of stem length of stecklings under T2 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 2 L water） and leaf area expansion of stecklings under T1 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 1 L water） was significantly higher （p 〈 0.05） compared with that under TO （no fertilizer）. The increment of collar diameter and root biomass production varied insignificantly among different fertilizer concentrations, although stecklings under T2 showed the better performance. The results suggest that rooting juvenile single-node leafy stem cuttings can be an effective mean of regenerating H. pubescens. The application of 0.1% IBA or without IBA is recommended for rooting juvenile leafy stem cuttings. Application of fertilizer appears necessary for subsequent growth of stecklings in polythene bags.展开更多
The present study, conducted during 2016 and 2017 seasons, aimed to investigate the effect of IBA on rooting of Piper betle L. stem cuttings (softwood and semi-hardwood). The experiment was undertaken in misting house...The present study, conducted during 2016 and 2017 seasons, aimed to investigate the effect of IBA on rooting of Piper betle L. stem cuttings (softwood and semi-hardwood). The experiment was undertaken in misting house field 2 UPM using the sand media to determine the adventitious roots initiation and development using the histological method. The cuttings were treated with different IBA concentrations (0, 500, 1000, 1500 and 2000 mg/L). The nodes explants were used in the development of a protocol for in vitro propagation of P. betle L., with different concentrations of Clorox with different times of immersion (20% Clorox 10 minutes, 30% Clorox 10 minutes, 20% Clorox 20 minutes, and 30% 20 minutes). In multiplication of the plantlets, Murashige and Skoog (MS) medium with different concentrations of BAP (0, 0.5, 1.0, 2.0 mg/L) were used to investigate the rooting of the explants. The results indicated that the types of the cuttings were different in the rooting capacity and the length of the roots. Moreover, it was found that in comparison with the control treatment, by a rise in the concentrations of the IBA, there was a significant upsurge in the rooting percentage, the root diameter, and the number of the roots. The results indicated that the types of cutting with 1000, 1500 and 2000 mg/L IBA perform better in the root percentage (100%) in the semi hardwood cuttings. The best results, however, were 2000 mg/L IBA in the semi hardwood cuttings, with the number of the roots to be 35.05, and the fresh weight of the roots to be 3.94 g, the dry weight of the roots to be 0.33 g, the length of the roots to be 391.88 cm, the roots diameter to be 1.21 mm, the surface area of the roots to be 121.83 cm2, and the root volume to be 2.99 cm3. Nonetheless, the optimal concentration of Clorox with the time immersion was 20% with the 20-minute immersion time, which produced a shoot induction percentage of 30% dead explants and a mean number of 70.00 shoots per explant and the optimal concentration of benzylaminopurine (BAP) at 1.0 mg/L. It is of note that a shoot induction percentage of 22.29% and a mean number of 4.1% number of auxiliary bud per treatment. P. betle shoots in MS medium without PGR MS (0.0) yielded a good rooting.展开更多
The study describes the scope of clonal propagation of guava (Psidium guajava Linn.) by stem cutting collected from mature stockplants. Cuttings were treated with 0, 0.2%, 0.4% and 0.8% IBA solution and rooted in the ...The study describes the scope of clonal propagation of guava (Psidium guajava Linn.) by stem cutting collected from mature stockplants. Cuttings were treated with 0, 0.2%, 0.4% and 0.8% IBA solution and rooted in the non-mist propagator. Rooted cuttings were allowed to grow in the polybags filled with soil and cow-dung mixed in the ratio of 3: 1 (by volume) for three months to assess the steckling capacity and initial growth performance. The study reveals that the species is amenable for clonal propagation by mature stem cutting. The highest rooting percentage (60%) was observed in the cuttings treated with 0.4% IBA solution followed by 0.2% IBA and the lowest was in controlled cuttings. The maximum number of primary root (32.7) was developed in the cuttings treated with 0.8% IBA solution followed by 0.4% IBA and the lowest was in the cuttings without IBA treatment. The highest survival percentage (70.9) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (58.3) was in the cuttings without any treatment. However, there was no significant variation in height growth of cutlings due to IBA treatments in rooting.展开更多
<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:&...<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:"">is considered as a potential source of edible protein, oil, and phenolic compounds with anti-inflammatory activity. The use of stem cutting in vegetative propagation and grafting is as indispensable tools for mass multiplication of superior genotypes and helps in improve planting yield and quality. The study was aimed to investigate the effect of different diameters (10 - 15, 16 - 25 and 26 - 35 mm) and different hormone concentrations of indo-butyric acid (0, 1.5, 3, 6 and 10 g/L), in the rooting of <i>Jatropha</i> <i>platyphylla</i> and to obtain axillary buds to performed grafts. Rooting efficiency was 80% in greenhouse conditions. Hormone concentration and diameter significantly affected the rooting and shooting ability of <i>Jatropha</i> <i>platyphylla</i> stem cuttings. Stem cuttings of 26 - 35 mm have the greatest number, length and dry root weight. 76% survival of the grafted plants was obtained. <span>This demonstrates the necessity to improve the conventional propagation methods</span> with appropriate scientific inputs for more economical and time efficient techniques for standard propagation protocols.展开更多
Shoots of Citrus sp. Kuharske were used to develop protocols for rooting reportedly HLB resistance rootstocks under intermittent mist. Investigated were shoot maturity, nodes per cutting, leaves per cutting, effects o...Shoots of Citrus sp. Kuharske were used to develop protocols for rooting reportedly HLB resistance rootstocks under intermittent mist. Investigated were shoot maturity, nodes per cutting, leaves per cutting, effects of buds, auxin concentrations and auxin solvent. Shoot maturity was most influential for success, with cuttings taken below the first 30 cm of active terminal growth producing greater root generation. Use of a thickening agent (Natrosal) to dilute the commercial auxin was second most in importance for rooting success. Root mass increased with increasing number of leaves. Cutting stems between nodes or below the lowest bud were inconsequential. To produce maximum number of viable cuttings, single node-single leaf cuttings were preferred. Single bud cuttings produced one shoot after rooting. This was adventitious since multi-node cuttings usually sprouted new shoots that would need to be removed before budded. Evaluation of the best combination of auxin and cutting-related attributes were evaluated with four additional common rootstocks in June 2016. Rooting was 100% successful. A quick dip (0.5 s) in a 7500 ppm solution of Dip&Gro produced the most root generation in six weeks for all rootstocks. Root quantity varied by rootstock.展开更多
文摘In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% IBA compared to control （0% IBA）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.
文摘Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid （NAA） @ 2000, 4000 （ppm） in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate （50%） was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control （talc powder） failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation ofA. indica under Kashmir conditions.
文摘Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000, 4000(ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar.The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments.The highest rooting rate(50%) was recorded in the cuttings with the application of IBA @ 4000 ppm.The cuttings treated with IBA @ 2000 ppm had 25% rooting rate.All other treatments along with control(talc powder) failed to induce rooting.It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A.indica under Kashmir conditions.
基金funded by the Natural Science Foundation of Inner Mongolia
文摘Techniques have been developed for the vegetative propagation of Tetraena mongolica,a rare and endangered species endemic to the western Ordos Plateau in the northcentral portion of China.The purpose of the study was to investigate the rooting characteris-tics of the plant species from the aspects of length and ages of cuttings,external hormone concentration,leaf retention,cutting orientations,and cutting positions.The results obtained from the experiments indicated that the rooting ratio of 2-year or 3-year old cuttings was larger than more than of 4-year old cuttings.The influence of external hormone concentration on rooting was ob-vious;the rooting ratio of cuttings was increased by external hormone treatment,especially when treated by ABT1(ABT No.1 Rooting Powder) 50 mg/L for 3 hours,in which case,the rooting ratio reached 91 percent.Leaf retention influenced rooting sig-nificantly;the rooting rate of cuttings became greater as the leaf retention increased.There was no significant difference in rooting capability between south and north orientations of the ortets.The rooting ratio of the cuttings obtained from the distal portions of shoots was significantly larger than those obtained from the middle and the basal portions of the shoots.On day three after treat-ment,calluses started appearing at the incisions of some cuttings dipped in 500 mg/L IAA(indole acetic acid).Most cuttings were healed or swelled,and 6 percent of cuttings rooted on day 6 after treatment.Higher rooting period occurred 7-10 days after the experiment was set up.
文摘The present study investigated the rooting ability and growth performance of juvenile single-node leafy stem cuttings of Holarrhena pubescens, collected from two mature mother trees preserved in the hill forests of Chittagong District, Bangladesh. The rooting ability of cuttings was studied with 0, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. The better rooting response was observed with 0.1% IBA, but was insignificant compared to other treatments. The mean number of roots and length of the longest root of cuttings in different treatments showed no significant difference. After transfer to the polythene bags from a nonmist propagator, rooted cuttings treated with 0.4% IBA demonstrated the highest （50.00± 0.78%） survival capacity. Regarding initial growth of established rooted cuttings （stecklings） over a 90 d period, the increment of stem length of stecklings under T2 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 2 L water） and leaf area expansion of stecklings under T1 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 1 L water） was significantly higher （p 〈 0.05） compared with that under TO （no fertilizer）. The increment of collar diameter and root biomass production varied insignificantly among different fertilizer concentrations, although stecklings under T2 showed the better performance. The results suggest that rooting juvenile single-node leafy stem cuttings can be an effective mean of regenerating H. pubescens. The application of 0.1% IBA or without IBA is recommended for rooting juvenile leafy stem cuttings. Application of fertilizer appears necessary for subsequent growth of stecklings in polythene bags.
文摘The present study, conducted during 2016 and 2017 seasons, aimed to investigate the effect of IBA on rooting of Piper betle L. stem cuttings (softwood and semi-hardwood). The experiment was undertaken in misting house field 2 UPM using the sand media to determine the adventitious roots initiation and development using the histological method. The cuttings were treated with different IBA concentrations (0, 500, 1000, 1500 and 2000 mg/L). The nodes explants were used in the development of a protocol for in vitro propagation of P. betle L., with different concentrations of Clorox with different times of immersion (20% Clorox 10 minutes, 30% Clorox 10 minutes, 20% Clorox 20 minutes, and 30% 20 minutes). In multiplication of the plantlets, Murashige and Skoog (MS) medium with different concentrations of BAP (0, 0.5, 1.0, 2.0 mg/L) were used to investigate the rooting of the explants. The results indicated that the types of the cuttings were different in the rooting capacity and the length of the roots. Moreover, it was found that in comparison with the control treatment, by a rise in the concentrations of the IBA, there was a significant upsurge in the rooting percentage, the root diameter, and the number of the roots. The results indicated that the types of cutting with 1000, 1500 and 2000 mg/L IBA perform better in the root percentage (100%) in the semi hardwood cuttings. The best results, however, were 2000 mg/L IBA in the semi hardwood cuttings, with the number of the roots to be 35.05, and the fresh weight of the roots to be 3.94 g, the dry weight of the roots to be 0.33 g, the length of the roots to be 391.88 cm, the roots diameter to be 1.21 mm, the surface area of the roots to be 121.83 cm2, and the root volume to be 2.99 cm3. Nonetheless, the optimal concentration of Clorox with the time immersion was 20% with the 20-minute immersion time, which produced a shoot induction percentage of 30% dead explants and a mean number of 70.00 shoots per explant and the optimal concentration of benzylaminopurine (BAP) at 1.0 mg/L. It is of note that a shoot induction percentage of 22.29% and a mean number of 4.1% number of auxiliary bud per treatment. P. betle shoots in MS medium without PGR MS (0.0) yielded a good rooting.
文摘The study describes the scope of clonal propagation of guava (Psidium guajava Linn.) by stem cutting collected from mature stockplants. Cuttings were treated with 0, 0.2%, 0.4% and 0.8% IBA solution and rooted in the non-mist propagator. Rooted cuttings were allowed to grow in the polybags filled with soil and cow-dung mixed in the ratio of 3: 1 (by volume) for three months to assess the steckling capacity and initial growth performance. The study reveals that the species is amenable for clonal propagation by mature stem cutting. The highest rooting percentage (60%) was observed in the cuttings treated with 0.4% IBA solution followed by 0.2% IBA and the lowest was in controlled cuttings. The maximum number of primary root (32.7) was developed in the cuttings treated with 0.8% IBA solution followed by 0.4% IBA and the lowest was in the cuttings without IBA treatment. The highest survival percentage (70.9) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (58.3) was in the cuttings without any treatment. However, there was no significant variation in height growth of cutlings due to IBA treatments in rooting.
文摘<i><span style="font-family:"">Jatropha</span></i><span style="font-family:""> <i>platyphylla</i> </span><span style="font-family:"">is considered as a potential source of edible protein, oil, and phenolic compounds with anti-inflammatory activity. The use of stem cutting in vegetative propagation and grafting is as indispensable tools for mass multiplication of superior genotypes and helps in improve planting yield and quality. The study was aimed to investigate the effect of different diameters (10 - 15, 16 - 25 and 26 - 35 mm) and different hormone concentrations of indo-butyric acid (0, 1.5, 3, 6 and 10 g/L), in the rooting of <i>Jatropha</i> <i>platyphylla</i> and to obtain axillary buds to performed grafts. Rooting efficiency was 80% in greenhouse conditions. Hormone concentration and diameter significantly affected the rooting and shooting ability of <i>Jatropha</i> <i>platyphylla</i> stem cuttings. Stem cuttings of 26 - 35 mm have the greatest number, length and dry root weight. 76% survival of the grafted plants was obtained. <span>This demonstrates the necessity to improve the conventional propagation methods</span> with appropriate scientific inputs for more economical and time efficient techniques for standard propagation protocols.
文摘Shoots of Citrus sp. Kuharske were used to develop protocols for rooting reportedly HLB resistance rootstocks under intermittent mist. Investigated were shoot maturity, nodes per cutting, leaves per cutting, effects of buds, auxin concentrations and auxin solvent. Shoot maturity was most influential for success, with cuttings taken below the first 30 cm of active terminal growth producing greater root generation. Use of a thickening agent (Natrosal) to dilute the commercial auxin was second most in importance for rooting success. Root mass increased with increasing number of leaves. Cutting stems between nodes or below the lowest bud were inconsequential. To produce maximum number of viable cuttings, single node-single leaf cuttings were preferred. Single bud cuttings produced one shoot after rooting. This was adventitious since multi-node cuttings usually sprouted new shoots that would need to be removed before budded. Evaluation of the best combination of auxin and cutting-related attributes were evaluated with four additional common rootstocks in June 2016. Rooting was 100% successful. A quick dip (0.5 s) in a 7500 ppm solution of Dip&Gro produced the most root generation in six weeks for all rootstocks. Root quantity varied by rootstock.
