Objectives:Vascular endothelial growth factor(VEGF)regulates tumor vascularization in response to hypoxia and inflammatory signals.The polyphenol curcumin is supposed to interfere with inflammation-induced VEGF secret...Objectives:Vascular endothelial growth factor(VEGF)regulates tumor vascularization in response to hypoxia and inflammatory signals.The polyphenol curcumin is supposed to interfere with inflammation-induced VEGF secretion and might therefore support anti-VEGF-based treatments.We aimed to investigate the interaction between curcumin and the inflammatory cytokine Interleukin-1β(IL-1β)for VEGF secretion in breast cancer cell lines representing major breast cancer subtypes.Methods:VEGF in cell cultures was detected by Western blot and enzyme-linked immunosorbent assay(ELISA).Kinase phosphorylation was investigated by Western blotting.Gene expressions were analyzed by correlation tests.VEGF was evaluated in a retrospective breast cancer cohort by immunohistochemistry.Survival analysis was performed by the Kaplan-Meier algorithm.Results:VEGF secretion and kinase signaling in response to IL-1βand curcumin varied significantly for the cell lines MCF-7(Luminal A),SK-BR-3(HER2/neu+),MDA-MB-231,and UACC-3199(triple negative breast cancer).All cell lines increased VEGF secretion under hypoxia,but IL-1βincreased VEGF secretion only in MCF-7 cells.Curcumin inhibited VEGF secretion in MDA-MB-231,but increased it in MCF-7 and UACC-3199 cells.Curcumin induced phosphorylation of extracellular signal-regulated kinase(ERK)and p38-mitogen-activated protein kinase(p38-MAPK).However,inhibitor experiments demonstrated that ERK was more important for VEGF secretion.In gene expression data from the METABRIC study,no clear correlation of hypoxia-induced factor(HIF1A),IL-1β,and VEGF mRNA expression was observed;however,a suggested crosstalk of hypoxia and inflammatory pathways was observed.Conclusion:These dissimilar responses of breast cancer cell lines suggest that therapy efficiency with anti-VEGF,anti-IL-1β,or curcumin will also vary within breast cancers.展开更多
Objective:To investigate the antifibrotic effects of curcumin in a transverse aortic constriction(TAC)mouse model and elucidate its molecular mechanisms.Methods:Male C57BL/6 mice underwent TAC and received vehicle,low...Objective:To investigate the antifibrotic effects of curcumin in a transverse aortic constriction(TAC)mouse model and elucidate its molecular mechanisms.Methods:Male C57BL/6 mice underwent TAC and received vehicle,low-dose curcumin(50 mg/kg),high-dose curcumin(200 mg/kg),high-dose curcumin plus a scrambled control antagomir,or high-dose curcumin plus anti-miR-29b treatments.Cardiac function was assessed by echocardiography.Fibrosis was evaluated by histology,collagen volume fraction,and hydroxyproline content.Expression of miR-29b,HDAC4,and fibrosis-related markers(Col1a1,Col3a1,TGF-β1)was measured by quantitative RT-PCR and Western blotting assays.Myocardial procollagen type I carboxy-terminal propeptide was determined by ELISA,and HDAC4-specific enzymatic activity was assayed using a fluorogenic kit.Results:Curcumin improved cardiac function,reduced fibrosis,restored miR-29b expression,and suppressed HDAC4 expression and activity in a dose-dependent manner.Furthermore,curcumin decreased myocardial procollagen type I carboxy-terminal propeptide levels,confirming reduced collagen synthesis.Anti-miR-29b administration partially abrogated the antifibrotic and cardioprotective effects of curcumin.Conclusions:Curcumin attenuates pressure overload-induced cardiac fibrosis and dysfunction in a TAC mouse model via modulation of the miR-29b/HDAC4 axis and suppression of collagen synthesis.展开更多
Vibrio harveyi,a Gram-negative bacterium ubiquitous in marine environments,is recognized as an opportunistic pathogen affecting various aquatic organisms such as fish,shrimp,and shellfish.To enhance its environmental ...Vibrio harveyi,a Gram-negative bacterium ubiquitous in marine environments,is recognized as an opportunistic pathogen affecting various aquatic organisms such as fish,shrimp,and shellfish.To enhance its environmental resilience and adaptive capacity,V.harveyi employs a complex quorum sensing mechanism to modulate its virulence factors,such as bioluminescence,biofilm formation,and motility.Therefore,targeting the quorum sensing of V.harveyi could be a promising strategy to develop novel approaches to ensure the microbial safety of seafood products.This study aims to evaluate the impact of curcumin-mediated photo/sonodynamic treatment on quorum sensing in V.harveyi and its regulated functions.The results indicate a significant decrease of luminescence in V.harveyi following curcumin-mediated photo/sonodynamic treatment.Correspondingly,the biofilm formation ability and bacterial motility of V.harveyi were also greatly impaired by the treatment.Notably,the production of reactive oxygen species in bacteria induced by the photo/sonodynamic treatment could be the underlying mechanism involved in the observed disruption of quorum sensing.These findings underscore the great potential of photo/sonodynamic treatment as a promising strategy to disrupt quorum sensing and mitigate the virulence of V.harveyi,thereby contributing to the development of effective control strategies against this pervasive pathogen.展开更多
Star-shaped six-arm polymers with hexaaza[2_(6)]orthoparacyclophane core and arms of block copolymers of poly-2-ethyl-5,6-dihydrooxazine with poly-2-isopropyl-5,6-dihydrooxazine were synthesized successfully using cat...Star-shaped six-arm polymers with hexaaza[2_(6)]orthoparacyclophane core and arms of block copolymers of poly-2-ethyl-5,6-dihydrooxazine with poly-2-isopropyl-5,6-dihydrooxazine were synthesized successfully using cationic ring-opening polymerization.The ratio of blocks,the order of their attachment to the core,and arm length were varied.Conformation of synthesized stars was determined by methods of molecular hydrodynamics and optics.It has been shown that star-shaped molecules were characterized by high intramolecular density,and the arm folding increased with their lengthening.The influence of the structure of block copolymers and their molar mass on the critical micelle concentration has been established.Complexes of synthesized star-shaped block copolymers with curcumin were obtained and the efficient binding of curcumin to polymer molecules was demonstrated.The behavior of the aqueous solutions of the prepared polymer stars and their complexes with curcumin was investigated by light scattering and turbidimetry methods.The influence of the structure and molar mass of star polymers on their thermoresponsiveness and the phase separation temperatures in aqueous solutions was analyzed.A slight increase in the phase separation temperature was found on passage from polymer solutions to solutions of polymer complexes with hydrophobic curcumin.展开更多
BACKGROUND Malignant meningioma metastasizes systemically,primarily due to its role in epithelial-mesenchymal transition.Although the prognosis is extremely poor,drug development efforts have been limited,because this...BACKGROUND Malignant meningioma metastasizes systemically,primarily due to its role in epithelial-mesenchymal transition.Although the prognosis is extremely poor,drug development efforts have been limited,because this tumor is categorized as a rare form.AIM To examine growth suppressive effect of GO-Y030,a diarylpentanoid curcumin analog,(1E,4E)-1,5-bis[3,5-bis(methoxymethoxy)phenyl]penta-1,4-dien-3-one against the malignant meningioma.METHODS The growth suppression of malignant meningioma cells by GO-Y022 and GOY030 were examined,using IOMM-Lee and HKBMM cell lines.Male nude mice aged eight weeks,specifically BALB/cSlc-nu/nu mice received a subcutaneous inoculation of IOMM-Lee(107 cells/site)on their back and 30μg/kg of recombinant hepatocellular growth factor(HGF)was injected into the tumor every three days.After confirmed the growth tumor mass,500μL of GO-Y030 diluted with PBS were administrated intraperitoneally daily at doses of 1 mg/kg and 2 mg/kg,respectively.RESULTS GO-Y030 exhibits a growth inhibitory effect on malignant meningioma cell lines,IOMM-Lee and HKBMM ranging from 0.8-2.0μM in vitro.Notably,GO-Y030’s inhibitory effect is about 10 to 16th times more potent than that of curcumin,which has previously demonstrated potential in combating malignant meningioma.In mouse models,the intraperitoneal administration of GO-Y030 effectively suppresses the growth of malignant meningioma tumors that have been inoculated in the back(P=0.002).High-performance liquid chromatography analysis has confirmed the distribution of GO-Y030 in the bloodstream and brain tissue.Moreover,GOY030 demonstrates the ability to significantly suppress HGF(P<0.01),nuclear factor kappa B(P<0.001),and Ncadherin(P<0.001),all of which contribute to the epithelial-mesenchymal transition.CONCLUSION GO-Y030 holds promise as a potent compound for the systemic inhibition of malignant meningioma.GO-Y030 has higher tumor growth inhibitory effect against meningiomas than curcumin,which is known to have antitumor activity through multi-molecular target control resulting in apoptosis induction.GO-Y030 controls at least three molecules of HGF,nuclear factor kappa B,and N-cadherin.展开更多
Objective:To assess the effects of turmeric extract and its compounds on oxidative stress,inflammation,and apoptosis in acetaminophen-induced liver injury.Methods:HepG2 cells were administered with acetaminophen(40 mM...Objective:To assess the effects of turmeric extract and its compounds on oxidative stress,inflammation,and apoptosis in acetaminophen-induced liver injury.Methods:HepG2 cells were administered with acetaminophen(40 mM)to induce hepatotoxicity,followed by treatment with turmeric extract and its isolated compounds including curcumin,demethoxycurcumin,bis-demethoxycurcumin and ar-turmerone at 5,25,and 125μg/mL.IL-1β,IL-6,and IL-10 levels were quantified with ELISA kits.Further,qRT-PCR was used to analyze the mRNA expression of JNK,Casp-9,and Casp-3.Meanwhile,the levels of nitric oxide and lactate dehydrogenase were analyzed using colorimetric assay.Results:Acetaminophen administration caused an increase in the levels of lactate dehydrogenase,nitric oxide,IL-1β,IL-6,and the mRNA expression of JNK,Casp-9,and Casp-3 in HepG2 cells while reducing IL-10 levels.Treatment with turmeric extract,curcumin,demethoxycurcumin,bis-demethoxycurcumin,and ar-turmerone lowered IL-1β,IL-6,nitric oxide,and lactate dehydrogenase levels,downregulated the mRNA expression of JNK,Casp-9,and Casp-3,and increased IL-10 levels.Conclusions:Turmeric extract and its compounds have significant hepatoprotective activity and could be further explored for the treatment of liver damage.展开更多
The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curc...The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.展开更多
The study by Yang et al presents a comprehensive investigation into the thera-peutic potential of curcumin for gastric cancer(GC).Using network pharma-cology,the researchers identified 48 curcumin-related genes,31 of ...The study by Yang et al presents a comprehensive investigation into the thera-peutic potential of curcumin for gastric cancer(GC).Using network pharma-cology,the researchers identified 48 curcumin-related genes,31 of which overlap with GC targets.Key genes,including ESR1,EGFR,CYP3A4,MAPK14,CYP1A2,and CYP2B6,are linked to poor survival in GC patients.Molecular docking con-firmed strong binding affinity of curcumin to these genes.In vitro experiments demonstrated that curcumin effectively inhibits the growth and proliferation of BGC-823,suggesting its therapeutic potential in GC through multiple targets and pathways.展开更多
Parkinson's disease(PD)is a common clinical nervous system disease,which seriously affects the health of middle—aged and elderly people.The current treatment for Parkinson's disease is only aimed at alleviati...Parkinson's disease(PD)is a common clinical nervous system disease,which seriously affects the health of middle—aged and elderly people.The current treatment for Parkinson's disease is only aimed at alleviating early symptoms.Curcumin(CUR)is a natural product,which has been widely used in anti—in—flammation,anticancer,and other fields.However,the potential mechanism of CUR in treating PD is currently unclear.The corresponding genes of CUR were harvested from the TCMSP,SwissTargetPrediction,SuperPred,PharmMapper,and SEA.Meanwhile,genes associated with PD were adopted from OMIM,TTD,DrugBank,MalaCards,and GeneCards databases.Through Gene Ontology(GO)and Kyoto Encyclopaedia of Genes and Genomes(KEGG)pathway enrichment analyses,therapeutic targeting KEGG pathways and functions were further collected.Then,STRING was used to generate the protein—protein interaction(PPI)network.The"drug—targets—disease"network was built by Cystoscope.Finally,the binding between CUR and core targets of PD was identified via molecular docking technology.The network pharmacology analysis revealed 65 potential targets related to the treatment of PD with CUR.The 10 core targets are CYP3A4,GSK3B,CYP19A1,CHEK1,COMT,CYP2A6,CYP2C19,HSD17B1,NFE2L2,and AHR.Moreover,KEGG enrichment analysis found that it was closely related to metabolic pathways,glycosaminoglycan degradation,steroid hormone biosynthesis,etc.The molecular docking results revealed that CUR had a strong binding ability with the PD target protein.In vitro experiments have shown that CUR can significantly improve oxidative damage caused by rotenone intervention in PD SHSY5Y cells and upregulate the level of key antioxidant pathway proteins Nrf2 and AHR.In a word,CUR may have therapeutic effects on PD through multitarget and multi—pathway,which provide a scientificbasis for further elaborating the mechanism of CUR in the treatment of PD.展开更多
Nitrogen-phosphorus co-doped carbon dots(N,P-CDs)were synthesized via a one-step hydrothermal method using p-phenylene diisocyanate,ethylenediamine,and concentrated phosphoric acid as raw materials.The morphology,stru...Nitrogen-phosphorus co-doped carbon dots(N,P-CDs)were synthesized via a one-step hydrothermal method using p-phenylene diisocyanate,ethylenediamine,and concentrated phosphoric acid as raw materials.The morphology,structure,and optical properties of the N,P-CDs were characterized in detail.The N,P-CDs exhibit excellent water solubility,with optimal excitation and emission wavelengths of 392 nm and 520 nm,respectively.A novel fluorescence method for detection of curcumin was developed,demonstrating a linear range of 3.50-242.24μmol/L and a detection limit of 0.086μmol/L.When applied to the detection of curcumin in real water samples and chili powder seasoning,the method achieved recovery between 94.48%and 105.82%,and with the relative standard deviations(RSDs)ranging from 0.17%to 0.62%.These results highlight that the constructed fluorescence analysis method based on the N,P-CDs has the potential for the accurate and reliable detection of curcumin in real-world samples.展开更多
Micellar nanostructures formed by amphiphilic polymers are prone to dissociation when the in vivo environment changes.Polyprodrug micelles can cross-link with other hydrophobic drugs through noncovalent bonds,which ha...Micellar nanostructures formed by amphiphilic polymers are prone to dissociation when the in vivo environment changes.Polyprodrug micelles can cross-link with other hydrophobic drugs through noncovalent bonds,which has the advantage of fixed structure and avoids the use of chemical cross-linking agents.In this study,we prepared a polyprodrug with hydrophobic curcumin(CUR)and hydrophilic poly(ethylene glycol)(PEG)in the main chain through a click reaction between CUR derivatives containing azide groups and di-alkynly-capped PEG.Due to the presence of benzene rings in the structure of CUR,the polyprodrug can form non-covalent cross-linked nanoparticles(NCCL-CUR NPs)through hydrophobic andπ-πstacking interaction.The structure,molecular weight,and self-assembly properties of the polyprodrug were characterized.The anti-cancer drug camptothecin(CPT)was encapsulated in the polyprodrug nanoparticles,producing dual-drug-loaded nanoparticles(abbreviated as CPT@NCCL-CUR NPs).The test results indicate that the NPs have reductive responsiveness and can release the original drugs CUR and CPT in phosphate buffer(PB)solution containing glutathione(GSH),while remaining stability in physiological environment.Cell and in vivo experiments further demonstrate that the dualdrug-loaded CPT@NCCL-CUR NPs can inhibit the growth of tumor through synergistic effects.This work provides a valuable approach for the preparation of amphiphilic polyprodrug with anti-tumor CUR as the backbone,and the stable dual-drug-loaded NPs containing both CUR and CPT through non-covalent cross-linking for synergistic therapy.展开更多
We prepared curcumin(Cur)/carboxymethyl-β-cyclodextrin(CM-β-CD)complex by grinding method.According to the characteristics of the tumor microenvironment,a pH-responsive nanogel loaded with Cur was designed and prepa...We prepared curcumin(Cur)/carboxymethyl-β-cyclodextrin(CM-β-CD)complex by grinding method.According to the characteristics of the tumor microenvironment,a pH-responsive nanogel loaded with Cur was designed and prepared(by CM-β-CD and chitosan)and consequently characterized by DLS,TEM,FT-IR,~1H NMR,SEM,etc.In vitro release results show that Cur-loaded Chitosan-CM-β-CD nanogel(Cur-CS-CM-β-CD)released Cur rapidly under acidic conditions,and its cumulative release rate is 41%,56%and 67%at pH 7.4,6.5 and 5.5,respectively.The cell inhibition rate of Cur-CS-CM-β-CD on MCF-7 cell lines was detected by the MTT assay.The results suggest the cell inhibition rate of Cur-CS-CM-β-CD is(50.2±2.5)%at 10μM,(98.3±1.2)%at 40μM and(97.5±1.2)%at 80μM,respectively.It is revealed that the pH-responsive nanogel loaded Cur can effectively inhibit the growth of breast cancer cells and has the potential for clinical application.展开更多
Background Ochratoxin A(OTA)is a toxin widely found in aquafeed ingredients,and hypoxia is a common prob-lem in fish farming.In practice,aquatic animals tend to be more sensitive to hypoxia while feeds are contaminate...Background Ochratoxin A(OTA)is a toxin widely found in aquafeed ingredients,and hypoxia is a common prob-lem in fish farming.In practice,aquatic animals tend to be more sensitive to hypoxia while feeds are contaminated with OTA,but no studies exist in this area.This research investigated the multiple biotoxicities of OTA and hypoxia combined on the liver of grass carp and explored the mitigating effect of curcumin(CUR).Methods A total of 720 healthy juvenile grass carp(11.06±0.05 g)were selected and assigned randomly to 4 experi-mental groups:control group(without OTA and CUR),1.2 mg/kg OTA group,400 mg/kg CUR group,and 1.2 mg/kg OTA+400 mg/kg CUR group with three replicates each for 60 d.Subsequently,32 fish were selected,divided into nor-moxia(18 fish)and hypoxia(18 fish)groups,and subjected to hypoxia stress for 96 h.Results CUR can attenuate histopathological damage caused by coming to OTA and hypoxia by reducing vacu-olation and nuclear excursion.The alleviation of this damage was associated with the attenuation of apoptosis in the mitochondrial pathway by decreasing the expression of the pro-apoptotic proteins Caspase 3,8,9,Bax,and Apaf1 while increasing the expression of the anti-apoptotic protein Bcl-2,and attenuation of endoplasmic reticulum stress(ERS)by reducing Grp78 expression and chop levels.This may be attributed to the fact that the addi-tion of CUR increased the levels of catalase(CAT)and glutathione reductase(GSH),increased antioxidant capacity,and ensured the proper functioning of respiratory chain complexes I and II,which in turn reduced the high produc-tion of reactive oxygen species(ROS),thus alleviating apoptosis and ERS.Conclusions In conclusion,our data demonstrate the effectiveness of CUR in attenuating liver injury caused by the combination of OTA and hypoxia.This study confirms the feasibility and efficacy of adding natural products to mitigate toxic damage to aquatic animals.展开更多
The prevalence of ulcerative colitis(UC)is increasing annually,while current non-targeted drugs for UC have limited effectiveness,easily relapsed,and serious side effects.Herein,curcumin(Cur)-loaded nanoparticle with ...The prevalence of ulcerative colitis(UC)is increasing annually,while current non-targeted drugs for UC have limited effectiveness,easily relapsed,and serious side effects.Herein,curcumin(Cur)-loaded nanoparticle with conlon-targeted property based on Mesona chinensis polysaccharides(MCP)was developed for the synergistic and targeted improvement of UC.Results show that MCP-zein nanoparticles(ZmNPs)have good encapsulation of Cur,targeted delivery of Cur to the colon,and prolonged its retention time.In vivo safety assessments have shown that ZmNPs have good safety and biocompatibility.As expected,Cur-ZmNPs effectively alleviated the symptoms of Dextran sulfate sodium(DSS)-induced UC by decreasing colonic inflammation by inhibiting the TLR4/MAPK pathway,regulating the levels of oxidative stress and immune homeostasis of UC mice.Oral administration of Cur-ZmNPs can reduce apoptosis of intestinal epithelial cells,alleviate colonic mucosal damage and repair intestinal barrier function.Cur-ZmNPs also had a positive effect on improving gut microbiota disorders and promoting the production of SCFAs.This study provides a novel strategy for synergistic alleviation of UC by MCP-based NPs loaded with food bioactives.展开更多
Objective:To observed the effect of a curcumin-based vaginal gel combined with electroporation for the treatment of vulvovaginal candidiasis(VVC)caused by Candida albicans.Methods:Temperature-sensitive in situ gels(IS...Objective:To observed the effect of a curcumin-based vaginal gel combined with electroporation for the treatment of vulvovaginal candidiasis(VVC)caused by Candida albicans.Methods:Temperature-sensitive in situ gels(ISG)were prepared using poloxamers 407 and 188 as matrices.The mass ratio of poloxamer 407 and poloxamer 188 was 7:1 with a gelation temperature of approximately 29℃ and gelation time of 2.5 min.Results:Electroporation increased the transmucosal permeability of the model drug,doxorubicin and improved the antifungal effects of curcumin.In vitro antifungal experiments showed that the number of fungal colonies in curcumin ISG combined with electroporation was lower than that in pure curcumin ISG.In vivo pharmacodynamic experiments showed that,compared to the model group,curcumin ISG with electroporation inhibited the growth of C.albicans,alleviated vaginal mucosal edema,and reduced the inflammatory response.Conclusion:Curcumin ISG combined with electroporation has substantial potential for the efficient clinical treatment of VVC.展开更多
Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,w...Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,we introduced a novel nanotherapeutic approach involving a curcumin-loaded ceria nanoenzyme delivery system tailored to counteract the multifaceted aspects of ALI.This system leverages the individual and combined effects of the components to provide a comprehensive therapeutic solution.The dual-action capability of this nanosystem was manifested by mitigating mitochondrial oxidative stress in lung epithelial cells and inhibiting the transient receptor potential melanosome-associated protein 2(TRPM2)-NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway,offering a highly effective therapeutic approach to ALI.Our findings reveal the underlying mechanisms of this innovative nanodelivery system,showcasing its potential as a versatile strategy for ALI treatment and encouraging further exploration of nanoenzyme-based therapies for ALI.展开更多
Soil salinization is a major abiotic stress that hampers plant development and significantly reduces agricultural productivity,posing a serious challenge to global food security.Akebia trifoliata(Thunb.)Koidz,a specie...Soil salinization is a major abiotic stress that hampers plant development and significantly reduces agricultural productivity,posing a serious challenge to global food security.Akebia trifoliata(Thunb.)Koidz,a species within the genus Akebia Decne.,is valued for its use in food,traditionalmedicine,oil production,and as an ornamental plant.Curcumin,widely recognized for its pharmacological properties including anti-cancer,anti-neuroinflammatory,and anti-fibrotic effects,has recently drawn interest for its potential roles in plant stress responses.However,its impact on plant tolerance to saline-alkali stress remains poorly understood.In this study,the effects of curcumin on saline-alkali resistance in A.trifoliata were examined by subjecting plants to a saline-alkali solution containing 150 mmol/L sodium ions(a mixture of Na_(2)SO_(4),Na_(2)CO_(3),and NaHCO_(3)).Curcumin treatment under these stress conditions leads to anatomical improvements in leaf structure.Furthermore,A.trifoliatamaintained a favorable Na^(+)/K^(+)ratio through increased potassium uptake and reduced sodium accumulation.Biochemical analysis revealed elevated levels of proline,soluble sugars,and soluble proteins,along with improved activities of antioxidant enzymes such as superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD).Similarly,the concentrations of hydrogen peroxide(H_(2)O_(2))and malondialdehyde(MDA)were significantly reduced.Transcriptome analysis under saline-alkali stress conditions showed that curcumin influenced seven keymetabolic pathways annotated in the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,with differentially expressed unigenes primarily enriched in transcription factor families such as MYB,AP2/ERF,NAC,bHLH,and C2C2.Moreover,eight differentially expressed genes(DEGs)associated with plant hormone signal transduction were linked to the auxin and brassinosteroid pathways,critical for cell elongation and plant growth.These findings indicate that curcumin increases saline-alkali stress tolerance in A.trifoliata by modulating physiological,biochemical,and transcriptional responses,ultimately supporting improved growth under adverse conditions.展开更多
The global incidence of oral cancer has steadily increased in recent years and is associated with high morbidity and mortality.Oral cancer is the most common cancer in the head and neck region,and is predominantly of ...The global incidence of oral cancer has steadily increased in recent years and is associated with high morbidity and mortality.Oral cancer is the most common cancer in the head and neck region,and is predominantly of epithelial origin(i.e.squamous cell carcinoma).Oral cancer treatment modalities mainly include surgery with or without radiotherapy and chemotherapy.Though proven effective,chemotherapy has significant adverse effects with possibilities of tumor resistance to anticancer drugs and recurrence.Thus,there is an imperative need to identify suitable anticancer therapies that are highly precise with minimal side effects and to make oral cancer treatment effective and safer.Among the available adjuvant therapies is curcumin,a plant polyphenol isolated from the rhizome of the turmeric plant Curcuma longa.Curcumin has been demonstrated to have antiinfectious,antioxidant,anti-inflammatory,and anticarcinogenic properties.Curcumin has poor bioavailability,which has been overcome by its various analogues and nanoformulations,such as nanoparticles,liposome complexes,micelles,and phospholipid complexes.Studies have shown that the anticancer effects of curcumin are mediated by its action on multiple molecular targets,including activator protein 1,protein kinase B(Akt),nuclear factorκ-light-chainenhancer of activated B cells,mitogen-activated protein kinase,epidermal growth factor receptor(EGFR)expression,and EGFR downstream signaling pathways.These targets play important roles in oral cancer pathogenesis,thereby making curcumin a promising adjuvant treatment modality.This review aims to summarize the different novel formulations of curcumin and their role in the treatment of oral cancer.展开更多
Objective：To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods：The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 wa...Objective：To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods：The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 was evaluated by the canine kidney cells （MDCK）, Results：The largest non toxic concentration of curcumin extract was 12, 5g/L and the effective inhibitory concentration to H1N1 and H3N2 was 6, 25G/1 AND 1,56g/L respectively, Conclusion： Curcumin extract have directly killing effect on H1N1 and H3N2 infections.展开更多
Cancer of the prostate gland is a leading cause of death. In the present study, we studied the effects and mechanisms of curcumin analogue E10, docetaxel or their combination on prostate cancer （PC）-3 cells. Treatme...Cancer of the prostate gland is a leading cause of death. In the present study, we studied the effects and mechanisms of curcumin analogue E10, docetaxel or their combination on prostate cancer （PC）-3 cells. Treatment of PC-3 cells with El0 or docetaxel resulted in growth inhibition in a concentration-reliant fashion. Combinations of E10 and docetaxel inhibited the growth of PC-3 cells in a synergistic manner. Effects of a combination of E10 and docetaxel were associated with synergistic inhibition of the transcriptional activity of nuclear factor-kappa B （NF-KB）, and robust reductions in the levels of B-cell lymphoma-2 （Bcl-2） were found in PC-3 cells treated with a combination of E10 and docetaxel. Our data indicated that the effects of El0 in combination with docetaxel on PC-3 cells were associated with inhibition of NF-r,B and Bcl-2. Further studies using suitable animal models are necessary to determine the in vivo effect of this combination.展开更多
基金funded by the Deutsche Forschungsgemeinschaft(DFG)grant number(KA2663/3-1)supported by the Ministry of Science,Research and Cultural Affairs of the State of Brandenburg.
文摘Objectives:Vascular endothelial growth factor(VEGF)regulates tumor vascularization in response to hypoxia and inflammatory signals.The polyphenol curcumin is supposed to interfere with inflammation-induced VEGF secretion and might therefore support anti-VEGF-based treatments.We aimed to investigate the interaction between curcumin and the inflammatory cytokine Interleukin-1β(IL-1β)for VEGF secretion in breast cancer cell lines representing major breast cancer subtypes.Methods:VEGF in cell cultures was detected by Western blot and enzyme-linked immunosorbent assay(ELISA).Kinase phosphorylation was investigated by Western blotting.Gene expressions were analyzed by correlation tests.VEGF was evaluated in a retrospective breast cancer cohort by immunohistochemistry.Survival analysis was performed by the Kaplan-Meier algorithm.Results:VEGF secretion and kinase signaling in response to IL-1βand curcumin varied significantly for the cell lines MCF-7(Luminal A),SK-BR-3(HER2/neu+),MDA-MB-231,and UACC-3199(triple negative breast cancer).All cell lines increased VEGF secretion under hypoxia,but IL-1βincreased VEGF secretion only in MCF-7 cells.Curcumin inhibited VEGF secretion in MDA-MB-231,but increased it in MCF-7 and UACC-3199 cells.Curcumin induced phosphorylation of extracellular signal-regulated kinase(ERK)and p38-mitogen-activated protein kinase(p38-MAPK).However,inhibitor experiments demonstrated that ERK was more important for VEGF secretion.In gene expression data from the METABRIC study,no clear correlation of hypoxia-induced factor(HIF1A),IL-1β,and VEGF mRNA expression was observed;however,a suggested crosstalk of hypoxia and inflammatory pathways was observed.Conclusion:These dissimilar responses of breast cancer cell lines suggest that therapy efficiency with anti-VEGF,anti-IL-1β,or curcumin will also vary within breast cancers.
基金supported by China International Medical Foundation(Z-2019-42-1908-4)Natural Science Basic Research Program of Shaanxi Province(2019JM-440).
文摘Objective:To investigate the antifibrotic effects of curcumin in a transverse aortic constriction(TAC)mouse model and elucidate its molecular mechanisms.Methods:Male C57BL/6 mice underwent TAC and received vehicle,low-dose curcumin(50 mg/kg),high-dose curcumin(200 mg/kg),high-dose curcumin plus a scrambled control antagomir,or high-dose curcumin plus anti-miR-29b treatments.Cardiac function was assessed by echocardiography.Fibrosis was evaluated by histology,collagen volume fraction,and hydroxyproline content.Expression of miR-29b,HDAC4,and fibrosis-related markers(Col1a1,Col3a1,TGF-β1)was measured by quantitative RT-PCR and Western blotting assays.Myocardial procollagen type I carboxy-terminal propeptide was determined by ELISA,and HDAC4-specific enzymatic activity was assayed using a fluorogenic kit.Results:Curcumin improved cardiac function,reduced fibrosis,restored miR-29b expression,and suppressed HDAC4 expression and activity in a dose-dependent manner.Furthermore,curcumin decreased myocardial procollagen type I carboxy-terminal propeptide levels,confirming reduced collagen synthesis.Anti-miR-29b administration partially abrogated the antifibrotic and cardioprotective effects of curcumin.Conclusions:Curcumin attenuates pressure overload-induced cardiac fibrosis and dysfunction in a TAC mouse model via modulation of the miR-29b/HDAC4 axis and suppression of collagen synthesis.
基金financially supported by National Natural Science Foundation of China(32272450)Science Fund for Distinguished Young Scholars of Fujian Province(2023J06020)Special Funds for Science and Technology Innovation of Fujian Agriculture and Forestry University(KFB23132A)。
文摘Vibrio harveyi,a Gram-negative bacterium ubiquitous in marine environments,is recognized as an opportunistic pathogen affecting various aquatic organisms such as fish,shrimp,and shellfish.To enhance its environmental resilience and adaptive capacity,V.harveyi employs a complex quorum sensing mechanism to modulate its virulence factors,such as bioluminescence,biofilm formation,and motility.Therefore,targeting the quorum sensing of V.harveyi could be a promising strategy to develop novel approaches to ensure the microbial safety of seafood products.This study aims to evaluate the impact of curcumin-mediated photo/sonodynamic treatment on quorum sensing in V.harveyi and its regulated functions.The results indicate a significant decrease of luminescence in V.harveyi following curcumin-mediated photo/sonodynamic treatment.Correspondingly,the biofilm formation ability and bacterial motility of V.harveyi were also greatly impaired by the treatment.Notably,the production of reactive oxygen species in bacteria induced by the photo/sonodynamic treatment could be the underlying mechanism involved in the observed disruption of quorum sensing.These findings underscore the great potential of photo/sonodynamic treatment as a promising strategy to disrupt quorum sensing and mitigate the virulence of V.harveyi,thereby contributing to the development of effective control strategies against this pervasive pathogen.
基金financially supported by the Russian Science Foundation(No.23-13-00205)。
文摘Star-shaped six-arm polymers with hexaaza[2_(6)]orthoparacyclophane core and arms of block copolymers of poly-2-ethyl-5,6-dihydrooxazine with poly-2-isopropyl-5,6-dihydrooxazine were synthesized successfully using cationic ring-opening polymerization.The ratio of blocks,the order of their attachment to the core,and arm length were varied.Conformation of synthesized stars was determined by methods of molecular hydrodynamics and optics.It has been shown that star-shaped molecules were characterized by high intramolecular density,and the arm folding increased with their lengthening.The influence of the structure of block copolymers and their molar mass on the critical micelle concentration has been established.Complexes of synthesized star-shaped block copolymers with curcumin were obtained and the efficient binding of curcumin to polymer molecules was demonstrated.The behavior of the aqueous solutions of the prepared polymer stars and their complexes with curcumin was investigated by light scattering and turbidimetry methods.The influence of the structure and molar mass of star polymers on their thermoresponsiveness and the phase separation temperatures in aqueous solutions was analyzed.A slight increase in the phase separation temperature was found on passage from polymer solutions to solutions of polymer complexes with hydrophobic curcumin.
基金Supported by TAIHO Pharmaceutical,No.AS2023A000122715Nippon Kayaku,No.NKCS20230416001.
文摘BACKGROUND Malignant meningioma metastasizes systemically,primarily due to its role in epithelial-mesenchymal transition.Although the prognosis is extremely poor,drug development efforts have been limited,because this tumor is categorized as a rare form.AIM To examine growth suppressive effect of GO-Y030,a diarylpentanoid curcumin analog,(1E,4E)-1,5-bis[3,5-bis(methoxymethoxy)phenyl]penta-1,4-dien-3-one against the malignant meningioma.METHODS The growth suppression of malignant meningioma cells by GO-Y022 and GOY030 were examined,using IOMM-Lee and HKBMM cell lines.Male nude mice aged eight weeks,specifically BALB/cSlc-nu/nu mice received a subcutaneous inoculation of IOMM-Lee(107 cells/site)on their back and 30μg/kg of recombinant hepatocellular growth factor(HGF)was injected into the tumor every three days.After confirmed the growth tumor mass,500μL of GO-Y030 diluted with PBS were administrated intraperitoneally daily at doses of 1 mg/kg and 2 mg/kg,respectively.RESULTS GO-Y030 exhibits a growth inhibitory effect on malignant meningioma cell lines,IOMM-Lee and HKBMM ranging from 0.8-2.0μM in vitro.Notably,GO-Y030’s inhibitory effect is about 10 to 16th times more potent than that of curcumin,which has previously demonstrated potential in combating malignant meningioma.In mouse models,the intraperitoneal administration of GO-Y030 effectively suppresses the growth of malignant meningioma tumors that have been inoculated in the back(P=0.002).High-performance liquid chromatography analysis has confirmed the distribution of GO-Y030 in the bloodstream and brain tissue.Moreover,GOY030 demonstrates the ability to significantly suppress HGF(P<0.01),nuclear factor kappa B(P<0.001),and Ncadherin(P<0.001),all of which contribute to the epithelial-mesenchymal transition.CONCLUSION GO-Y030 holds promise as a potent compound for the systemic inhibition of malignant meningioma.GO-Y030 has higher tumor growth inhibitory effect against meningiomas than curcumin,which is known to have antitumor activity through multi-molecular target control resulting in apoptosis induction.GO-Y030 controls at least three molecules of HGF,nuclear factor kappa B,and N-cadherin.
基金funded by Maranatha Christian University,Bandung,Indonesia for Productive Lecturer Research under grant number:011/SK/ADD/UKM/IV/2024.
文摘Objective:To assess the effects of turmeric extract and its compounds on oxidative stress,inflammation,and apoptosis in acetaminophen-induced liver injury.Methods:HepG2 cells were administered with acetaminophen(40 mM)to induce hepatotoxicity,followed by treatment with turmeric extract and its isolated compounds including curcumin,demethoxycurcumin,bis-demethoxycurcumin and ar-turmerone at 5,25,and 125μg/mL.IL-1β,IL-6,and IL-10 levels were quantified with ELISA kits.Further,qRT-PCR was used to analyze the mRNA expression of JNK,Casp-9,and Casp-3.Meanwhile,the levels of nitric oxide and lactate dehydrogenase were analyzed using colorimetric assay.Results:Acetaminophen administration caused an increase in the levels of lactate dehydrogenase,nitric oxide,IL-1β,IL-6,and the mRNA expression of JNK,Casp-9,and Casp-3 in HepG2 cells while reducing IL-10 levels.Treatment with turmeric extract,curcumin,demethoxycurcumin,bis-demethoxycurcumin,and ar-turmerone lowered IL-1β,IL-6,nitric oxide,and lactate dehydrogenase levels,downregulated the mRNA expression of JNK,Casp-9,and Casp-3,and increased IL-10 levels.Conclusions:Turmeric extract and its compounds have significant hepatoprotective activity and could be further explored for the treatment of liver damage.
文摘The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.
基金Supported by The College Students’Innovation and Entrepreneurship Competition,No.2024cxcy504 and No.202410459164.
文摘The study by Yang et al presents a comprehensive investigation into the thera-peutic potential of curcumin for gastric cancer(GC).Using network pharma-cology,the researchers identified 48 curcumin-related genes,31 of which overlap with GC targets.Key genes,including ESR1,EGFR,CYP3A4,MAPK14,CYP1A2,and CYP2B6,are linked to poor survival in GC patients.Molecular docking con-firmed strong binding affinity of curcumin to these genes.In vitro experiments demonstrated that curcumin effectively inhibits the growth and proliferation of BGC-823,suggesting its therapeutic potential in GC through multiple targets and pathways.
基金Supported by Shandong Natural Science Foundation General Project(ZR2022MH197)。
文摘Parkinson's disease(PD)is a common clinical nervous system disease,which seriously affects the health of middle—aged and elderly people.The current treatment for Parkinson's disease is only aimed at alleviating early symptoms.Curcumin(CUR)is a natural product,which has been widely used in anti—in—flammation,anticancer,and other fields.However,the potential mechanism of CUR in treating PD is currently unclear.The corresponding genes of CUR were harvested from the TCMSP,SwissTargetPrediction,SuperPred,PharmMapper,and SEA.Meanwhile,genes associated with PD were adopted from OMIM,TTD,DrugBank,MalaCards,and GeneCards databases.Through Gene Ontology(GO)and Kyoto Encyclopaedia of Genes and Genomes(KEGG)pathway enrichment analyses,therapeutic targeting KEGG pathways and functions were further collected.Then,STRING was used to generate the protein—protein interaction(PPI)network.The"drug—targets—disease"network was built by Cystoscope.Finally,the binding between CUR and core targets of PD was identified via molecular docking technology.The network pharmacology analysis revealed 65 potential targets related to the treatment of PD with CUR.The 10 core targets are CYP3A4,GSK3B,CYP19A1,CHEK1,COMT,CYP2A6,CYP2C19,HSD17B1,NFE2L2,and AHR.Moreover,KEGG enrichment analysis found that it was closely related to metabolic pathways,glycosaminoglycan degradation,steroid hormone biosynthesis,etc.The molecular docking results revealed that CUR had a strong binding ability with the PD target protein.In vitro experiments have shown that CUR can significantly improve oxidative damage caused by rotenone intervention in PD SHSY5Y cells and upregulate the level of key antioxidant pathway proteins Nrf2 and AHR.In a word,CUR may have therapeutic effects on PD through multitarget and multi—pathway,which provide a scientificbasis for further elaborating the mechanism of CUR in the treatment of PD.
基金National Natural Science Foundation of China(No.22274090)The Basic Research Project of Shanxi Province(No.202203021221026)+1 种基金Shanxi Province Patent Conversion Project(No.202306012)Shanxi Provincial Key Laboratory of Classical Prescription Strengthening Yang(No.202104010910011)。
文摘Nitrogen-phosphorus co-doped carbon dots(N,P-CDs)were synthesized via a one-step hydrothermal method using p-phenylene diisocyanate,ethylenediamine,and concentrated phosphoric acid as raw materials.The morphology,structure,and optical properties of the N,P-CDs were characterized in detail.The N,P-CDs exhibit excellent water solubility,with optimal excitation and emission wavelengths of 392 nm and 520 nm,respectively.A novel fluorescence method for detection of curcumin was developed,demonstrating a linear range of 3.50-242.24μmol/L and a detection limit of 0.086μmol/L.When applied to the detection of curcumin in real water samples and chili powder seasoning,the method achieved recovery between 94.48%and 105.82%,and with the relative standard deviations(RSDs)ranging from 0.17%to 0.62%.These results highlight that the constructed fluorescence analysis method based on the N,P-CDs has the potential for the accurate and reliable detection of curcumin in real-world samples.
基金supported by the National Natural Science Foundation of China(No.21975169)the Project Fund of the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions+2 种基金the Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function of Soochow Universitythe Research project of China Baoyuan Investment Co.,Ltd.Suzhou Science and Technology Plan Project(No.SKY2023051)。
文摘Micellar nanostructures formed by amphiphilic polymers are prone to dissociation when the in vivo environment changes.Polyprodrug micelles can cross-link with other hydrophobic drugs through noncovalent bonds,which has the advantage of fixed structure and avoids the use of chemical cross-linking agents.In this study,we prepared a polyprodrug with hydrophobic curcumin(CUR)and hydrophilic poly(ethylene glycol)(PEG)in the main chain through a click reaction between CUR derivatives containing azide groups and di-alkynly-capped PEG.Due to the presence of benzene rings in the structure of CUR,the polyprodrug can form non-covalent cross-linked nanoparticles(NCCL-CUR NPs)through hydrophobic andπ-πstacking interaction.The structure,molecular weight,and self-assembly properties of the polyprodrug were characterized.The anti-cancer drug camptothecin(CPT)was encapsulated in the polyprodrug nanoparticles,producing dual-drug-loaded nanoparticles(abbreviated as CPT@NCCL-CUR NPs).The test results indicate that the NPs have reductive responsiveness and can release the original drugs CUR and CPT in phosphate buffer(PB)solution containing glutathione(GSH),while remaining stability in physiological environment.Cell and in vivo experiments further demonstrate that the dualdrug-loaded CPT@NCCL-CUR NPs can inhibit the growth of tumor through synergistic effects.This work provides a valuable approach for the preparation of amphiphilic polyprodrug with anti-tumor CUR as the backbone,and the stable dual-drug-loaded NPs containing both CUR and CPT through non-covalent cross-linking for synergistic therapy.
基金Funded by the Tianjin Municipal Education Commission(No.2022ZD041)。
文摘We prepared curcumin(Cur)/carboxymethyl-β-cyclodextrin(CM-β-CD)complex by grinding method.According to the characteristics of the tumor microenvironment,a pH-responsive nanogel loaded with Cur was designed and prepared(by CM-β-CD and chitosan)and consequently characterized by DLS,TEM,FT-IR,~1H NMR,SEM,etc.In vitro release results show that Cur-loaded Chitosan-CM-β-CD nanogel(Cur-CS-CM-β-CD)released Cur rapidly under acidic conditions,and its cumulative release rate is 41%,56%and 67%at pH 7.4,6.5 and 5.5,respectively.The cell inhibition rate of Cur-CS-CM-β-CD on MCF-7 cell lines was detected by the MTT assay.The results suggest the cell inhibition rate of Cur-CS-CM-β-CD is(50.2±2.5)%at 10μM,(98.3±1.2)%at 40μM and(97.5±1.2)%at 80μM,respectively.It is revealed that the pH-responsive nanogel loaded Cur can effectively inhibit the growth of breast cancer cells and has the potential for clinical application.
基金financially supported by the earmarked fund for CARS(CARS-45)National Natural Science Foundation of China(32273144,32072985)National Key R&D Program of China(2019YFD0900200).
文摘Background Ochratoxin A(OTA)is a toxin widely found in aquafeed ingredients,and hypoxia is a common prob-lem in fish farming.In practice,aquatic animals tend to be more sensitive to hypoxia while feeds are contaminated with OTA,but no studies exist in this area.This research investigated the multiple biotoxicities of OTA and hypoxia combined on the liver of grass carp and explored the mitigating effect of curcumin(CUR).Methods A total of 720 healthy juvenile grass carp(11.06±0.05 g)were selected and assigned randomly to 4 experi-mental groups:control group(without OTA and CUR),1.2 mg/kg OTA group,400 mg/kg CUR group,and 1.2 mg/kg OTA+400 mg/kg CUR group with three replicates each for 60 d.Subsequently,32 fish were selected,divided into nor-moxia(18 fish)and hypoxia(18 fish)groups,and subjected to hypoxia stress for 96 h.Results CUR can attenuate histopathological damage caused by coming to OTA and hypoxia by reducing vacu-olation and nuclear excursion.The alleviation of this damage was associated with the attenuation of apoptosis in the mitochondrial pathway by decreasing the expression of the pro-apoptotic proteins Caspase 3,8,9,Bax,and Apaf1 while increasing the expression of the anti-apoptotic protein Bcl-2,and attenuation of endoplasmic reticulum stress(ERS)by reducing Grp78 expression and chop levels.This may be attributed to the fact that the addi-tion of CUR increased the levels of catalase(CAT)and glutathione reductase(GSH),increased antioxidant capacity,and ensured the proper functioning of respiratory chain complexes I and II,which in turn reduced the high produc-tion of reactive oxygen species(ROS),thus alleviating apoptosis and ERS.Conclusions In conclusion,our data demonstrate the effectiveness of CUR in attenuating liver injury caused by the combination of OTA and hypoxia.This study confirms the feasibility and efficacy of adding natural products to mitigate toxic damage to aquatic animals.
基金supported by the National Key Research and Development Program of China(2023YFF1104001)Natural Science Foundation of Jiangxi Province,China(20232BCD44003).
文摘The prevalence of ulcerative colitis(UC)is increasing annually,while current non-targeted drugs for UC have limited effectiveness,easily relapsed,and serious side effects.Herein,curcumin(Cur)-loaded nanoparticle with conlon-targeted property based on Mesona chinensis polysaccharides(MCP)was developed for the synergistic and targeted improvement of UC.Results show that MCP-zein nanoparticles(ZmNPs)have good encapsulation of Cur,targeted delivery of Cur to the colon,and prolonged its retention time.In vivo safety assessments have shown that ZmNPs have good safety and biocompatibility.As expected,Cur-ZmNPs effectively alleviated the symptoms of Dextran sulfate sodium(DSS)-induced UC by decreasing colonic inflammation by inhibiting the TLR4/MAPK pathway,regulating the levels of oxidative stress and immune homeostasis of UC mice.Oral administration of Cur-ZmNPs can reduce apoptosis of intestinal epithelial cells,alleviate colonic mucosal damage and repair intestinal barrier function.Cur-ZmNPs also had a positive effect on improving gut microbiota disorders and promoting the production of SCFAs.This study provides a novel strategy for synergistic alleviation of UC by MCP-based NPs loaded with food bioactives.
基金supported by the Beijing Natural Science Foundation(L222126).
文摘Objective:To observed the effect of a curcumin-based vaginal gel combined with electroporation for the treatment of vulvovaginal candidiasis(VVC)caused by Candida albicans.Methods:Temperature-sensitive in situ gels(ISG)were prepared using poloxamers 407 and 188 as matrices.The mass ratio of poloxamer 407 and poloxamer 188 was 7:1 with a gelation temperature of approximately 29℃ and gelation time of 2.5 min.Results:Electroporation increased the transmucosal permeability of the model drug,doxorubicin and improved the antifungal effects of curcumin.In vitro antifungal experiments showed that the number of fungal colonies in curcumin ISG combined with electroporation was lower than that in pure curcumin ISG.In vivo pharmacodynamic experiments showed that,compared to the model group,curcumin ISG with electroporation inhibited the growth of C.albicans,alleviated vaginal mucosal edema,and reduced the inflammatory response.Conclusion:Curcumin ISG combined with electroporation has substantial potential for the efficient clinical treatment of VVC.
基金funded by the National Natural Science Foundation of China(Nos.82103885,81871521,82273672)Natural Science Foundation of Shanghai(Nos.21ZR1477700,20ZR1470300)+1 种基金the Shanghai Municipal Health Commission-Outstanding Youth Foundation of Public Health(No.GWV-10.2-YQ48)Sci Tech Funding by CSPFTZ Lingang Special Area Marine Biomedical Innovation Platform。
文摘Acute lung injury(ALI)is a critical respiratory disorder with a high mortality rate and is caused by several factors.Addressing oxidative stress and infiammation is a pivotal strategy for ALI treatment.In this study,we introduced a novel nanotherapeutic approach involving a curcumin-loaded ceria nanoenzyme delivery system tailored to counteract the multifaceted aspects of ALI.This system leverages the individual and combined effects of the components to provide a comprehensive therapeutic solution.The dual-action capability of this nanosystem was manifested by mitigating mitochondrial oxidative stress in lung epithelial cells and inhibiting the transient receptor potential melanosome-associated protein 2(TRPM2)-NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway,offering a highly effective therapeutic approach to ALI.Our findings reveal the underlying mechanisms of this innovative nanodelivery system,showcasing its potential as a versatile strategy for ALI treatment and encouraging further exploration of nanoenzyme-based therapies for ALI.
基金supported by the National Natural Science Foundation of China(Number:32060645)The Joint Special Project(Key Project)of Yunnan Province Local Undergraduate University(202101BA070001-036)+2 种基金The Joint Special Project(Surface Project)of Yunnan Province Local Undergraduate University(202101BA070001-172)the Science Research Fund Project for Education Department of Yunnan Province(Numbers:2023Y0876,2023Y0860,2023J0828)the Basic Research Special Project for Science and Technology Department of Yunnan Provincial(Number:202301AU070137).
文摘Soil salinization is a major abiotic stress that hampers plant development and significantly reduces agricultural productivity,posing a serious challenge to global food security.Akebia trifoliata(Thunb.)Koidz,a species within the genus Akebia Decne.,is valued for its use in food,traditionalmedicine,oil production,and as an ornamental plant.Curcumin,widely recognized for its pharmacological properties including anti-cancer,anti-neuroinflammatory,and anti-fibrotic effects,has recently drawn interest for its potential roles in plant stress responses.However,its impact on plant tolerance to saline-alkali stress remains poorly understood.In this study,the effects of curcumin on saline-alkali resistance in A.trifoliata were examined by subjecting plants to a saline-alkali solution containing 150 mmol/L sodium ions(a mixture of Na_(2)SO_(4),Na_(2)CO_(3),and NaHCO_(3)).Curcumin treatment under these stress conditions leads to anatomical improvements in leaf structure.Furthermore,A.trifoliatamaintained a favorable Na^(+)/K^(+)ratio through increased potassium uptake and reduced sodium accumulation.Biochemical analysis revealed elevated levels of proline,soluble sugars,and soluble proteins,along with improved activities of antioxidant enzymes such as superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD).Similarly,the concentrations of hydrogen peroxide(H_(2)O_(2))and malondialdehyde(MDA)were significantly reduced.Transcriptome analysis under saline-alkali stress conditions showed that curcumin influenced seven keymetabolic pathways annotated in the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,with differentially expressed unigenes primarily enriched in transcription factor families such as MYB,AP2/ERF,NAC,bHLH,and C2C2.Moreover,eight differentially expressed genes(DEGs)associated with plant hormone signal transduction were linked to the auxin and brassinosteroid pathways,critical for cell elongation and plant growth.These findings indicate that curcumin increases saline-alkali stress tolerance in A.trifoliata by modulating physiological,biochemical,and transcriptional responses,ultimately supporting improved growth under adverse conditions.
文摘The global incidence of oral cancer has steadily increased in recent years and is associated with high morbidity and mortality.Oral cancer is the most common cancer in the head and neck region,and is predominantly of epithelial origin(i.e.squamous cell carcinoma).Oral cancer treatment modalities mainly include surgery with or without radiotherapy and chemotherapy.Though proven effective,chemotherapy has significant adverse effects with possibilities of tumor resistance to anticancer drugs and recurrence.Thus,there is an imperative need to identify suitable anticancer therapies that are highly precise with minimal side effects and to make oral cancer treatment effective and safer.Among the available adjuvant therapies is curcumin,a plant polyphenol isolated from the rhizome of the turmeric plant Curcuma longa.Curcumin has been demonstrated to have antiinfectious,antioxidant,anti-inflammatory,and anticarcinogenic properties.Curcumin has poor bioavailability,which has been overcome by its various analogues and nanoformulations,such as nanoparticles,liposome complexes,micelles,and phospholipid complexes.Studies have shown that the anticancer effects of curcumin are mediated by its action on multiple molecular targets,including activator protein 1,protein kinase B(Akt),nuclear factorκ-light-chainenhancer of activated B cells,mitogen-activated protein kinase,epidermal growth factor receptor(EGFR)expression,and EGFR downstream signaling pathways.These targets play important roles in oral cancer pathogenesis,thereby making curcumin a promising adjuvant treatment modality.This review aims to summarize the different novel formulations of curcumin and their role in the treatment of oral cancer.
文摘Objective：To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods：The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 was evaluated by the canine kidney cells （MDCK）, Results：The largest non toxic concentration of curcumin extract was 12, 5g/L and the effective inhibitory concentration to H1N1 and H3N2 was 6, 25G/1 AND 1,56g/L respectively, Conclusion： Curcumin extract have directly killing effect on H1N1 and H3N2 infections.
基金The National Cancer Institute of China(Grant No.P30-CA072720)the National Natural Science Foundation of China(Gr ant No.81272452)the PhD Start-up Fund of Natural Science Foundation of Guangdong Province,China(Grant No.2014A030310329)
文摘Cancer of the prostate gland is a leading cause of death. In the present study, we studied the effects and mechanisms of curcumin analogue E10, docetaxel or their combination on prostate cancer （PC）-3 cells. Treatment of PC-3 cells with El0 or docetaxel resulted in growth inhibition in a concentration-reliant fashion. Combinations of E10 and docetaxel inhibited the growth of PC-3 cells in a synergistic manner. Effects of a combination of E10 and docetaxel were associated with synergistic inhibition of the transcriptional activity of nuclear factor-kappa B （NF-KB）, and robust reductions in the levels of B-cell lymphoma-2 （Bcl-2） were found in PC-3 cells treated with a combination of E10 and docetaxel. Our data indicated that the effects of El0 in combination with docetaxel on PC-3 cells were associated with inhibition of NF-r,B and Bcl-2. Further studies using suitable animal models are necessary to determine the in vivo effect of this combination.
