Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to deter...Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to determine if their combination(TG)could alleviate depression-like behaviors and hippocampal neuronal damage in a chronic unpredictable mild stress(CUMS)mouse model.Male C57BL/6J mice were divided into control,CUMS model,and CUMS+TG groups with varying doses.The CUMS group displayed depressionlike behaviors,including reduced activity and sucrose preference.TG treatment partially reversed these changes,significantly increasing antioxidant enzyme activities,decreasing pro-inflammatory cytokine levels,and improving neuromodulator levels.RNA-seq analysis identified the transthyretin(TTR)gene,upregulated in the model group but downregulated after TG treatment.TG treatment modulated intestinal microbiota composition compared to the CUMS group,including increased Firmicutes,reduced Bacteroidetes and Prevotella,and variable changes in Bifidobacterium abundance.In conclusion,our study indicates that CUMS exposure upregulates stress hormones and TTR expression,associated with neuroinflammation,oxidative stress,monoamine depletion,depression-like behaviors,and intestinal microbiota dysbiosis.TG treatment alleviates these effects and modulates intestinal microbiota,suggesting L-theanine and geniposide's potential as a novel depression therapy.展开更多
Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms tha...Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.展开更多
基金funded by Innovation Project for Chinese Academy of Agricultural Sciences(CAAS-ASTIP-TRI)China Agriculture Research System of MOF and MARA(CARS-19)。
文摘Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to determine if their combination(TG)could alleviate depression-like behaviors and hippocampal neuronal damage in a chronic unpredictable mild stress(CUMS)mouse model.Male C57BL/6J mice were divided into control,CUMS model,and CUMS+TG groups with varying doses.The CUMS group displayed depressionlike behaviors,including reduced activity and sucrose preference.TG treatment partially reversed these changes,significantly increasing antioxidant enzyme activities,decreasing pro-inflammatory cytokine levels,and improving neuromodulator levels.RNA-seq analysis identified the transthyretin(TTR)gene,upregulated in the model group but downregulated after TG treatment.TG treatment modulated intestinal microbiota composition compared to the CUMS group,including increased Firmicutes,reduced Bacteroidetes and Prevotella,and variable changes in Bifidobacterium abundance.In conclusion,our study indicates that CUMS exposure upregulates stress hormones and TTR expression,associated with neuroinflammation,oxidative stress,monoamine depletion,depression-like behaviors,and intestinal microbiota dysbiosis.TG treatment alleviates these effects and modulates intestinal microbiota,suggesting L-theanine and geniposide's potential as a novel depression therapy.
文摘Based on multidisciplinary research methods,this study analyzes the mechanisms and potential of Gastrodiae Rhizoma(Tianma)and Uncariae Ramulus Cum Uncis compatibility in treating hypertension.The research confirms that components including rhynchophylline and gastrodin exert antihypertensive effects by inhibiting vascular smooth muscle proliferation and modulating calcium signaling pathways.The combination of these two herbs enhances the bioavailability of core components,synergistically regulates targets such as PTGS2 and NOS3,activates the PPAR signaling pathway,and strengthens vasodilation and antioxidant capacity.Clinical studies demonstrate that Tianma Gouteng Decoction exhibits superior efficacy in improving mitochondrial function and delaying vascular aging compared to single components.This investigation provides scientific evidence for the multi-target antihypertensive effects of traditional Chinese medicine and promotes its modern application in cardiovascular disease treatment.
