To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCE...To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.展开更多
Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future ex...Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Sehmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages （eggs to adults）. The population ex- hinted an average developmental time of 13.6 d in conditions corresponding to the natural environment （water temperature 20~C salinity 15）. A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isoehrysis galbana was more favored for the reproduction of cope- pods than Phaeodactylum tricornutum, and 10xl0%ellsmL-1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of oftspring to initiate a new generation.展开更多
[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discuss...[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.展开更多
Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-savi...Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.展开更多
Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, th...Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, the question on whether MRJPs possess growth-factor-like activity on all types of cell cultures remains. In order to determine whether MRJPs can be used as an alternative to fetal bovine serum （FBS） in different types of human cell culture, the prolif- eration of the complex serum with different ratios of MRJPs/FBS （M/F） was evaluated on five cell lines： 293T, HFL-I, 231, HCT116, and Changliver using MTT （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） assay. The proliferation activity of the combination of the complex M/F serum with cytokines on the test cell lines was also measured. The results demonstrated that the complex serum with M/F 6/4 possessed the highest proliferation activity similar to or in excess of FBS. However, no activity of complex medium with M/F 6/4 was observed in 231 cells, indicating a selectivity of MRJPs on cell types. Compared with the complex medium with M/F 6/4, the complex medium with M/F 6/4 together with two cytokines, epidermal growth factor （EGF） and insulin-transferrin-selenium （ITS）, pro- moted proliferations of Changliver, 293T, HCT116, and H FL-I by 18.73%-56.19% （P〈0.01）. Our findings demonstrate that MRJPs could partially replace FBS in culturing many human cell lines.展开更多
The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7...The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.展开更多
CSCs （Cancer stem cells） have been involved in tumor resistance, metastasis and recurrence. In breast cancer, tumor cells are characterized by CD44＋, CD24-/low and ALDH 1 expression represents a subpopulation of BC...CSCs （Cancer stem cells） have been involved in tumor resistance, metastasis and recurrence. In breast cancer, tumor cells are characterized by CD44＋, CD24-/low and ALDH 1 expression represents a subpopulation of BCSC （breast cancer stem cell）. Several three-dimensional （3D） in vitro culturing cancer cells have been used to stimulate BCSC phenotype. The present study aimed to evaluate 3D cell culture in alginate matrix and the CD44, CD24 and ALDH1 mRNA levels of BCSC markers. The 3D culture was performed using MDA-MB-231 breast cancer cell line on alginate matrix 1.2% in RPMI medium. Expression of BCSC markers was evaluated by Real Time PCR （Polymerase Chain Reaction） comparing 3D to 2D culture. The 3D cultures increase of CD44 and CD24 mRNA levels and induce ALDH1 expression comparing to 2D culture. The data suggest that 3D alginate matrix alters the mRNA levels of genes involved in the phenotypic characteristics of BCSC.展开更多
The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered wit...The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered with particle-detachable superhydrophobic coatings that serve as both the substrates and particle sources for LM production.A produced LM,which serves as aminireactor for the 3D culture,features a monolayer nanoparticulate shell and naked-droplet-like transparency.The LM-inplate platform provides a double-superhydrophobic environment that increases the stability of the 3D culture and reduces the necessary operational cautions.In addition,both cell observation and high-throughput applications can be conducted in situ,owing to the high LM transparency and the multiwell structure,respectively.This platform integrates the advantages of naked droplets(transparent and clean),LMs(stable nonwetting),and multiwell plates(high-throughput capability)and thus is promising for labor-saving and versatile 3D culturing.展开更多
In vitro evaluation of novel therapeutic approaches often fails to reliably predict efficacy and toxicity,especially when recapitulating conditions involving recirculating cells.Current testing strategies are often ba...In vitro evaluation of novel therapeutic approaches often fails to reliably predict efficacy and toxicity,especially when recapitulating conditions involving recirculating cells.Current testing strategies are often based on static co-culturing of cells in suspension and 3D tissue models,where cell sedimentation on the target tissue can occur.The observed effects may then mostly be a consequence of sedimentation and of the corresponding forced cell-tissue interactions.The realization of continuous medium flow helps to better recapitulate physiological conditions and cell-tissue interactions.To tackle current limitations of perfused organ-on-chip approaches,we developed a microfluidic chip and operation concept,which prevents undesired sedimentation and accumulation of suspended cells during multiple days by relying on gravity-driven perfusion.Our platform,which we termed“human immune flow(hiFlow)chip”,enables to co-culture cells in suspension with up to 7 preformed microtissue models.Here,we present the design principle and operation of the platform,and we validate its performance by culturing cells and microtissues of a variety of different origins.Cells and tissues could be monitored on chip via high-resolution microscopy,while cell suspensions and microtissues could be easily retrieved for off-chip analysis.Our results demonstrate that primary immune cells and a range of different spheroid models of healthy and diseased tissues can be maintained for over 6 days on chip.As proof-of-concept cell-tissue interaction assay,we used an antibody treatment against diffuse midline glioma,a highly aggressive pediatric tumor.We are confident that our platform will help to increase the prediction power of in vitro preclinical testing of novel therapeutics that rely on the interaction of circulating cells with organ tissues.展开更多
Bryopsis hypnoides Lamouroux was regenerated In vitro from the protoplasm squeezed out from wild algae and the regenerated Individuals had an advantage over Indlvlduala from the wild In terms of growth. Culturing of s...Bryopsis hypnoides Lamouroux was regenerated In vitro from the protoplasm squeezed out from wild algae and the regenerated Individuals had an advantage over Indlvlduala from the wild In terms of growth. Culturing of segments of thalll also showed that segments from the regenerated algae grew better than those from individuals from the wild. The segment that corresponds to a part of a cell, at least a part of the protoplasm, can develop Into a mature Individual, Including rhlzold and thallus, suggesting that a multlnucleate alga, such as B. hypnoides, Is different from higher plants, whose totlpotancy Is based on an Intact cell （or protoplasm）. Further cultivation of mature Individuals from segments Indicated that the organelles In the thallus had two ways In which they could survive when the alga was on the decline： （Ⅰ） the organelles were transferred Into a ball with a gelatinous envelope through a formed pipe and the ball seemed to be capable of being propagated; and （Ⅱ） the organelles were aggregated In the thallus and then moved to the outside. An Interesting result Is that one organelle aggregation located outside the thallus germinated and developed Into a mature alga, although most organelle aggregations gradually lost vitality and died. The results of the present study reveal that the aggregation of organelles can regenerate a stronger organism than Individuals from the wild owing to the complete exchange of genetic material and may possibly enable organelles to survive in unfavorable surroundings.展开更多
At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guan...At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.展开更多
A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,...A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,and profound religious and cultural significance.With the acceleration of globalization,this symbol of Tibetan culture that combines artistic expression with spirituality has become a bridge for cultural exchange between the East and the West.Recently,China Today spoke to Yixi Puncog,art collector and council member of the China Association for Preservation and Development of Tibetan Culture,to learn more about Thangka art,its role in international exchange,and how it is enhancing China’s cultural soft power.展开更多
Mai Weitong,an illustrator and motion-graphic artist,based in London,the United Kingdom,has been drawing since she was a child,immersed in both Eastern and Western artistic environments.In 2017,after she graduated fro...Mai Weitong,an illustrator and motion-graphic artist,based in London,the United Kingdom,has been drawing since she was a child,immersed in both Eastern and Western artistic environments.In 2017,after she graduated from Camberwell College of Arts,in the UK,with a bachelor’s degree in illustration,Mai became a freelance illustrator,and she began collaborating with various publishers and advertising agencies to create book illustrations and promotional artwork.In her work,she has revitalized Eastern elements with contemporary illustrations.Through her illustrations,Mai creates bridges of cross-cultural communication,which she uses to help introduce talented Asian illustrators to international audiences.展开更多
A digital project recounts the creation of the most comprehensive book collection in Chinese history and the efforts to protect it from the ravages of war As the 2025 World Internet Conference Wuzhen Summit concluded ...A digital project recounts the creation of the most comprehensive book collection in Chinese history and the efforts to protect it from the ravages of war As the 2025 World Internet Conference Wuzhen Summit concluded last November in East China’s Zhejiang Province,the role of digital technologies in transforming cultural heritage preservation and transmission came into focus.展开更多
Nanjing,capital city of present-day Jiangsu Province and once the capital of six dynasties,is home to a rich collection of cultural relics that bear witness to thousands of years of Chinese civilization.
In 2021,the relatives of 16 international friends of China jointly sent a letter to Chinese President Xi Jinping,congratulating the Communist Party of China(CPC)on its 100th anniversary.These are the sons and daughter...In 2021,the relatives of 16 international friends of China jointly sent a letter to Chinese President Xi Jinping,congratulating the Communist Party of China(CPC)on its 100th anniversary.These are the sons and daughters and family members of those who helped China in its times of need.Eric Foster,nephew of renowned U.S.journalist Helen Foster-Snow,was one of them.展开更多
A New Chapter of the Century-Old Palace Museum Oriental Outlook Issue 24,2025 The Palace Museum,the imperial palace of the Ming and Qing dynasties(1368-1911),opened to the public in 1925.Rather than a group of static ...A New Chapter of the Century-Old Palace Museum Oriental Outlook Issue 24,2025 The Palace Museum,the imperial palace of the Ming and Qing dynasties(1368-1911),opened to the public in 1925.Rather than a group of static ancient buildings,it stands today as a dynamic cultural organism filled with invaluable collections of cultural relics preserved by generations of artisans and now displayed to the world through digital technology in long-lasting exhibitions.展开更多
Years ago,when I brought my friend from Germany on a visit to China,we immediately hit a road bump when we explored past the lights of the big cities for an authentic rural China experience.We had prepared for roadblo...Years ago,when I brought my friend from Germany on a visit to China,we immediately hit a road bump when we explored past the lights of the big cities for an authentic rural China experience.We had prepared for roadblocks like language barriers and hotels,but one that we never prepared for was food.展开更多
Blending tradition with modern elements,Songyang’s tea industry attracts young entrepreneurs to revive culture and drive economic growth.SONGYANG has a long and rich history of tea cultivation and production.The coun...Blending tradition with modern elements,Songyang’s tea industry attracts young entrepreneurs to revive culture and drive economic growth.SONGYANG has a long and rich history of tea cultivation and production.The county became famous for its tea as early as the Three Kingdoms period(220-280).Today,this land is home to over 10,000 hectares of ecofriendly tea plantations.One in every three Songyang residents is involved in the tea industry,whose full industrial chain value now exceeds RMB 13.5 billion.展开更多
基金NationalMedicine 863Project (No .2 0 0 2AA2Z3 43C)
文摘To prepare a hand-made micropore membrane culture plate insert forco-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane.After seeding brain capillary endothelial cells (BCECs) on it (under the effect ofastrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelialelectrical resistance (TEER). Results The plate insert has a stability of at least 15 d underculture condition. TEER increased significantly under co-culture condition from (66.1 +- 13.3)Ωcm^2 to (182.2 +- 6.7) Ωcm^2. Conclusion This micropore membrane culture plate insert can beeasily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable andrecyclable. It follows that the plate insert is a useful tool for co-culture and the relatedresearch fields.
基金supported by the National Basic Key Research Program (973) under the Ministry of ScienceTechnology of the People’s Republic of China (2005CB4223)by Qingdao Shinan District Science and Technology Administration (snkjjh2006020).
文摘Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Sehmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages （eggs to adults）. The population ex- hinted an average developmental time of 13.6 d in conditions corresponding to the natural environment （water temperature 20~C salinity 15）. A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isoehrysis galbana was more favored for the reproduction of cope- pods than Phaeodactylum tricornutum, and 10xl0%ellsmL-1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of oftspring to initiate a new generation.
基金Support by Major Science and Technology Projects of Guangdong Province (2004A20102002)~~
文摘[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.
基金the Researchers supporting Project number(RSP-2019/98)King Saud University,Riyadh,Saudi Arabia。
文摘Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.
基金Project supported by the National Natural Science Foundation of China(No.31271848)the Important Scientific&Technical Innovation Project of Hangzhou(No.20131812A25)the Foundation of Fuli Institute of Food Science of Zhejiang University(No.KY201404),China
文摘Royal jelly （R J） is a well-known bioactive substance. It contains large amounts of major royal jelly proteins （MRJPs）, which express growth-factor-like activity in several animal and human cell lines. However, the question on whether MRJPs possess growth-factor-like activity on all types of cell cultures remains. In order to determine whether MRJPs can be used as an alternative to fetal bovine serum （FBS） in different types of human cell culture, the prolif- eration of the complex serum with different ratios of MRJPs/FBS （M/F） was evaluated on five cell lines： 293T, HFL-I, 231, HCT116, and Changliver using MTT （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide） assay. The proliferation activity of the combination of the complex M/F serum with cytokines on the test cell lines was also measured. The results demonstrated that the complex serum with M/F 6/4 possessed the highest proliferation activity similar to or in excess of FBS. However, no activity of complex medium with M/F 6/4 was observed in 231 cells, indicating a selectivity of MRJPs on cell types. Compared with the complex medium with M/F 6/4, the complex medium with M/F 6/4 together with two cytokines, epidermal growth factor （EGF） and insulin-transferrin-selenium （ITS）, pro- moted proliferations of Changliver, 293T, HCT116, and H FL-I by 18.73%-56.19% （P〈0.01）. Our findings demonstrate that MRJPs could partially replace FBS in culturing many human cell lines.
基金Supported by Nonprofit Institute Research Grant of Guangxi Province(GK15-2,GK16-2)Guangxi Aquatic Animal Husbandry Science and Technology Project(GYMK201528030,201633034)~~
文摘The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.
文摘CSCs （Cancer stem cells） have been involved in tumor resistance, metastasis and recurrence. In breast cancer, tumor cells are characterized by CD44＋, CD24-/low and ALDH 1 expression represents a subpopulation of BCSC （breast cancer stem cell）. Several three-dimensional （3D） in vitro culturing cancer cells have been used to stimulate BCSC phenotype. The present study aimed to evaluate 3D cell culture in alginate matrix and the CD44, CD24 and ALDH1 mRNA levels of BCSC markers. The 3D culture was performed using MDA-MB-231 breast cancer cell line on alginate matrix 1.2% in RPMI medium. Expression of BCSC markers was evaluated by Real Time PCR （Polymerase Chain Reaction） comparing 3D to 2D culture. The 3D cultures increase of CD44 and CD24 mRNA levels and induce ALDH1 expression comparing to 2D culture. The data suggest that 3D alginate matrix alters the mRNA levels of genes involved in the phenotypic characteristics of BCSC.
基金National Natural Science Foundation of China,Grant/Award Numbers:52473323,11974280Projects of Interdisciplinary,NWPU,Grant/Award Number:0202022GH0306+2 种基金Natural Science Basic Research Plan in Shaanxi Province of China,Grant/Award Number:2024JC-YBMS-605Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2023A1515030047National Undergraduate Training Programs for Innovation and Entrepreneurship,Grant/Award Number:S202210699549。
文摘The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered with particle-detachable superhydrophobic coatings that serve as both the substrates and particle sources for LM production.A produced LM,which serves as aminireactor for the 3D culture,features a monolayer nanoparticulate shell and naked-droplet-like transparency.The LM-inplate platform provides a double-superhydrophobic environment that increases the stability of the 3D culture and reduces the necessary operational cautions.In addition,both cell observation and high-throughput applications can be conducted in situ,owing to the high LM transparency and the multiwell structure,respectively.This platform integrates the advantages of naked droplets(transparent and clean),LMs(stable nonwetting),and multiwell plates(high-throughput capability)and thus is promising for labor-saving and versatile 3D culturing.
基金the support for flow cytometry and microscopy by the single cell facility(SCF)at the Department of Biosystems Science and Engineering at ETH Zurichfinancially supported by the Innosuisse grant 38880.1 IP-LS.by the“Personalized Health and Related Technologies(PHRT)”of the ETH Domain(Project#2021-351).
文摘In vitro evaluation of novel therapeutic approaches often fails to reliably predict efficacy and toxicity,especially when recapitulating conditions involving recirculating cells.Current testing strategies are often based on static co-culturing of cells in suspension and 3D tissue models,where cell sedimentation on the target tissue can occur.The observed effects may then mostly be a consequence of sedimentation and of the corresponding forced cell-tissue interactions.The realization of continuous medium flow helps to better recapitulate physiological conditions and cell-tissue interactions.To tackle current limitations of perfused organ-on-chip approaches,we developed a microfluidic chip and operation concept,which prevents undesired sedimentation and accumulation of suspended cells during multiple days by relying on gravity-driven perfusion.Our platform,which we termed“human immune flow(hiFlow)chip”,enables to co-culture cells in suspension with up to 7 preformed microtissue models.Here,we present the design principle and operation of the platform,and we validate its performance by culturing cells and microtissues of a variety of different origins.Cells and tissues could be monitored on chip via high-resolution microscopy,while cell suspensions and microtissues could be easily retrieved for off-chip analysis.Our results demonstrate that primary immune cells and a range of different spheroid models of healthy and diseased tissues can be maintained for over 6 days on chip.As proof-of-concept cell-tissue interaction assay,we used an antibody treatment against diffuse midline glioma,a highly aggressive pediatric tumor.We are confident that our platform will help to increase the prediction power of in vitro preclinical testing of novel therapeutics that rely on the interaction of circulating cells with organ tissues.
基金Supported by the National Natural Science Foundation of China （40476059, 30170499, 30250003 and 39890390）, the Knowledge Innovation Program of the Chinese Academy of Sciences （KZCX2-211） and the Hi-Tech Research and Development （863） Program of China （2004AA603220）.
文摘Bryopsis hypnoides Lamouroux was regenerated In vitro from the protoplasm squeezed out from wild algae and the regenerated Individuals had an advantage over Indlvlduala from the wild In terms of growth. Culturing of segments of thalll also showed that segments from the regenerated algae grew better than those from individuals from the wild. The segment that corresponds to a part of a cell, at least a part of the protoplasm, can develop Into a mature Individual, Including rhlzold and thallus, suggesting that a multlnucleate alga, such as B. hypnoides, Is different from higher plants, whose totlpotancy Is based on an Intact cell （or protoplasm）. Further cultivation of mature Individuals from segments Indicated that the organelles In the thallus had two ways In which they could survive when the alga was on the decline： （Ⅰ） the organelles were transferred Into a ball with a gelatinous envelope through a formed pipe and the ball seemed to be capable of being propagated; and （Ⅱ） the organelles were aggregated In the thallus and then moved to the outside. An Interesting result Is that one organelle aggregation located outside the thallus germinated and developed Into a mature alga, although most organelle aggregations gradually lost vitality and died. The results of the present study reveal that the aggregation of organelles can regenerate a stronger organism than Individuals from the wild owing to the complete exchange of genetic material and may possibly enable organelles to survive in unfavorable surroundings.
文摘At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.
文摘A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,and profound religious and cultural significance.With the acceleration of globalization,this symbol of Tibetan culture that combines artistic expression with spirituality has become a bridge for cultural exchange between the East and the West.Recently,China Today spoke to Yixi Puncog,art collector and council member of the China Association for Preservation and Development of Tibetan Culture,to learn more about Thangka art,its role in international exchange,and how it is enhancing China’s cultural soft power.
文摘Mai Weitong,an illustrator and motion-graphic artist,based in London,the United Kingdom,has been drawing since she was a child,immersed in both Eastern and Western artistic environments.In 2017,after she graduated from Camberwell College of Arts,in the UK,with a bachelor’s degree in illustration,Mai became a freelance illustrator,and she began collaborating with various publishers and advertising agencies to create book illustrations and promotional artwork.In her work,she has revitalized Eastern elements with contemporary illustrations.Through her illustrations,Mai creates bridges of cross-cultural communication,which she uses to help introduce talented Asian illustrators to international audiences.
文摘A digital project recounts the creation of the most comprehensive book collection in Chinese history and the efforts to protect it from the ravages of war As the 2025 World Internet Conference Wuzhen Summit concluded last November in East China’s Zhejiang Province,the role of digital technologies in transforming cultural heritage preservation and transmission came into focus.
文摘Nanjing,capital city of present-day Jiangsu Province and once the capital of six dynasties,is home to a rich collection of cultural relics that bear witness to thousands of years of Chinese civilization.
文摘In 2021,the relatives of 16 international friends of China jointly sent a letter to Chinese President Xi Jinping,congratulating the Communist Party of China(CPC)on its 100th anniversary.These are the sons and daughters and family members of those who helped China in its times of need.Eric Foster,nephew of renowned U.S.journalist Helen Foster-Snow,was one of them.
文摘A New Chapter of the Century-Old Palace Museum Oriental Outlook Issue 24,2025 The Palace Museum,the imperial palace of the Ming and Qing dynasties(1368-1911),opened to the public in 1925.Rather than a group of static ancient buildings,it stands today as a dynamic cultural organism filled with invaluable collections of cultural relics preserved by generations of artisans and now displayed to the world through digital technology in long-lasting exhibitions.
文摘Years ago,when I brought my friend from Germany on a visit to China,we immediately hit a road bump when we explored past the lights of the big cities for an authentic rural China experience.We had prepared for roadblocks like language barriers and hotels,but one that we never prepared for was food.
文摘Blending tradition with modern elements,Songyang’s tea industry attracts young entrepreneurs to revive culture and drive economic growth.SONGYANG has a long and rich history of tea cultivation and production.The county became famous for its tea as early as the Three Kingdoms period(220-280).Today,this land is home to over 10,000 hectares of ecofriendly tea plantations.One in every three Songyang residents is involved in the tea industry,whose full industrial chain value now exceeds RMB 13.5 billion.
