The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoo...The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor（i.e., fine screen room and sludge dewatering house） and two outdoor（i.e., aeration tank and control site） sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria.展开更多
Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes a...Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67μg·g-1).展开更多
The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of ...The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012.The 16 S rRNA sequences were analyzed from 187 isolated bacterial strains.Three phyla,29 genera and 56 species were identified.The three phyla were Actinobacteria,Firmicutes and Proteobacteria;the Proteobacteria included a-Proteobacteria,P-Proteobacteria and y-Proteobacteria.y-Proteobacteria,Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species.Gram-positive bacteria(Actinobacteria and Firmicutes) accounted for 57.8% of all types identified.There were nine dominant genera,including Curtobacterium,Staphylococcus,and Halomonas,and 14 dominant species including Curtobacterium flaccumfaciens,Curtobacterium pusillum,and Staphylococcus sciuri.Of the strains identified,87.2% were catalase positive or weakly positive.展开更多
Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixe...Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixed dune and sandy grassland were selected to investigate the soil fungal diversity using a traditional culture-dependent approach.ITS sequencing was applied to identify the fungal strains.The counts of culturable fungi increased significantly from mobile dune to sandy grassland along the gradient of sandy land restoration.The Shannon-Wiener,Simpson and Evenness indices of culturable fungi ranged from 1.26-1.71,0.22-0.37 and 0.83-0.87,respectively.A total of 27 fungal strains were isolated using dilution plate cultural technique.The 27 fungal isolates were clustered into three groups:Ascomycota,Basidiomycota and Mucoromycota at phylum level,indicating that Ascomycota was the dominant fungal phylum(88.9%of the total).The isolated fungi were grouped into 3 phyla,5 classes,6 orders,11 families and 13 genera.The results show that culturable fungi were diverse in sandy land soils and fungal isolates have potential function in lipid turnover,cellulose degradation and ethanol,glucose and fatty acid production.Future studies should be carried out to explore their ecological and biological function for degraded sandy land restoration.展开更多
Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem pro...Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation.This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils,and the interaction between bacteria and environmental factors.The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis.The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g,respectively,indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil.A total of 98 species belonging to 34 genera were identified,among which Arthrobacter,Bacillus,and Streptomyces were found dominantly and widely distributed.The community of culturable bacteria had obvious sample specificity,and the diversity in hypolithic soil was higher than that in peripheral soil.On the regional scale,the distribution of culturable bacteria and the environmental factors showed regular changes.On the local scale,the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.展开更多
[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable ...[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable bacteria present in the leaves,roots,and rhizosphere soil of both healthy areca palms and those exhibiting symptoms of YLD within the same orchard were conducted.Furthermore,a differential analysis of the bacterial community structure was conducted.[Results]The bacterial count was observed to be greater in healthy areca palm samples compared to those exhibiting disease symptoms.Furthermore,the diversity of bacterial species in healthy areca palm samples surpassed that found in diseased samples.Notably,the bacterial genera that exhibited significant differences between healthy and diseased areca palms included Bacillus velezensis,Paenibacillus validus,Alcaligenes faecalis,Burkholderia territorii,Pseudomonas aeruginosa,Bacillus amyloliquefaciens,etc.[Conclusions]This study may offer data support and technical guidance for the effective prevention and control of YLD in areca palm in the future.展开更多
Next-generation sequencing technologies have significantly advanced our comprehension of microbial diversity and ecological roles within fermented foods.However,culture-based approaches remain essential for a comprehe...Next-generation sequencing technologies have significantly advanced our comprehension of microbial diversity and ecological roles within fermented foods.However,culture-based approaches remain essential for a comprehensive understanding of these complex ecosystems.This study integrated culture-dependent and culture-independent techniques to elucidate the microbial diversity and flavor-forming potential of fungi in high-temperature Daqu,a critical solid-state fermentation process in Maotai-flavor Baijiu production.Through iterative cultivation strategies,we successfully isolated and identified 660 pure eukaryotic colonies,representing 33 genera and 58 species,from Daqu samples.This approach significantly improved cultivation efficiency from 20.7 to 63.2%.In addition,despite the optimization of ITS rRNA primer sets to enhance the detection of eukaryotic microorganisms,we still identified 21 genera(comprising 27 species)that were culturable but not detected by high-throughput sequencing analysis.Fermentation experiments demonstrated the robust growth and substantial ethyl acetate production potential of these fungal species,particularly Kluyveromyces marxianus,which exhibited exceptional performance at elevated temperatures(45℃).This study advanced culture-dependent techniques to improve the isolation of eukaryotic microorganisms from Daqu.These findings highlight the importance of culture-based approaches in characterizing microbial diversity and function.展开更多
To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were se...To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including tradi- tional culturing, rDNA internal transcribed spacer （ITS） sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shmblands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance （S）, Shannon-Wiener index （H）, Simpson dominance index （D）, and Pielou evenness index （3） of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66-2.71, 0.92, 0.95~）.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.展开更多
At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guan...At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.展开更多
China’s booming performing arts sector is reshaping cultural experiences and creating vibrant new spaces for leisure,tourism and creativity The year 2025 marked a period of vigorous expansion for China’s performing ...China’s booming performing arts sector is reshaping cultural experiences and creating vibrant new spaces for leisure,tourism and creativity The year 2025 marked a period of vigorous expansion for China’s performing arts sector,as the industry evolved from a traditional entertainment field into a comprehensive engine of cultural consumption.展开更多
University students,fashion models,street musicians,recent graduates…hundreds of aspiring singers gathered on the campus of the University of Nairobi in Kenya for a music audition.Some had guitars slung across their ...University students,fashion models,street musicians,recent graduates…hundreds of aspiring singers gathered on the campus of the University of Nairobi in Kenya for a music audition.Some had guitars slung across their backs,others drummed rhythms on hand drums,and a few performed melodies they had written on their phones.展开更多
In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of S...In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of Sunny Wu,Chairman of the Hong Kong Doyens’Club,resonates perfectly with the national development.展开更多
A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,...A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,and profound religious and cultural significance.With the acceleration of globalization,this symbol of Tibetan culture that combines artistic expression with spirituality has become a bridge for cultural exchange between the East and the West.Recently,China Today spoke to Yixi Puncog,art collector and council member of the China Association for Preservation and Development of Tibetan Culture,to learn more about Thangka art,its role in international exchange,and how it is enhancing China’s cultural soft power.展开更多
This paper divides the centuries-long communication of traditional Chinese medicine(TCM)in France into three progressive stages:the first stage(17th to 19th centuries),characterized by academic recognition and knowled...This paper divides the centuries-long communication of traditional Chinese medicine(TCM)in France into three progressive stages:the first stage(17th to 19th centuries),characterized by academic recognition and knowledge transmission;the second stage(mid-19th century to late 20th century),focusing on clinical verification and school-based innovation;and the third stage(late 20th century to the present),marked by cultural integration and pluralistic coexistence within systems.The introduction of TCM in France is a process of cross-cultural adaptation and coexistence driven by specific health needs in French society,such as chronic disease management and complementary therapies.The French medical community and sociocultural context have not only verified and interpreted TCM through an empirical scientific perspective,but also realized creative localization by developing indigenous schools such as electroacupuncture and auricular acupuncture.Eventually,through institutional measures including national certification,terminology standardization,and networked healthcare services,TCM has been deeply integrated into France’s medical system and social context,forming a stable ecosystem of cross-cultural adaptation and pluralistic coexistence.The French experience,which takes aligning with local health needs as the starting point,stimulating localized cultural innovation as the core,and promoting systematic integration into local systems as the support,presents a picture of traditional medicine serving modern society,the improvement of global health,as well as mutual learning and symbiosis among different civilizations.展开更多
At the Ningxia Museum,visitors can dive into the history of the mysterious Western Xia Dynasty,as well as cultural and ethnic exchanges that occured along the ancient Silk Road centuries ago.THE area of present-day Ni...At the Ningxia Museum,visitors can dive into the history of the mysterious Western Xia Dynasty,as well as cultural and ethnic exchanges that occured along the ancient Silk Road centuries ago.THE area of present-day Ningxia Hui Autonomous Region in northwest China was a vital corridor of the ancient Silk Road.Around a thousand years ago,Yuan Hao,leader of the Tangut people,established the Western Xia(also known as Xixia)Dynasty(1038-1227)on this land,which coexisted with the Song,Liao,and Jin dynasties across China for nearly 200 years.展开更多
Guided by the significant theoretical principle of the“Two Integrations”and grounded in Marxist cultural theory as its methodological basis,this paper constructs a bidirectional interpretative model linking“Yellow ...Guided by the significant theoretical principle of the“Two Integrations”and grounded in Marxist cultural theory as its methodological basis,this paper constructs a bidirectional interpretative model linking“Yellow River Culture”with“Cultural Confidence”.It proposes an integrated“Objective-Content-Path-Support”framework.Through the synergy of three-dimensional objectives,adaptation of stratified content,innovation in four-dimensional pathways,and support from a three-dimensional guarantee system,this framework establishes a closed-loop operational mechanism of“Curriculum-Practice-Evaluation-Feedback”.The study focuses on core issues in integrating Yellow River culture into university education practices,such as content construction,methodological pathways,and institutional guarantees.It aims to provide a systematic reference for universities to fulfill their fundamental task of“fostering virtue and cultivating talent”and to serve the national strategies for ecological protection and high-quality development in the Yellow River Basin.展开更多
Your comments are welcome!Please feel free to contact us with your personal stories or vivid pictures!Address:24 Baiwanzhuang St.,Xicheng Dist.,Beijing,100037 E-mail:chinatodaynews@aliyun.com.As Cyprus’newly-appointe...Your comments are welcome!Please feel free to contact us with your personal stories or vivid pictures!Address:24 Baiwanzhuang St.,Xicheng Dist.,Beijing,100037 E-mail:chinatodaynews@aliyun.com.As Cyprus’newly-appointed Ambassador to China,I have been deeply impressed since my arrival in Beijing last August by the warmth of the Chinese people,the country’s rich heritage of culture and history,and the dynamism of its young innovators.At various events,I have witnessed their relentless drive for self-improvement through innovation,which is a testament to China’s strong emphasis on education,technology,and progress.展开更多
Pioneering initiatives in Yunnan Province are leveraging intangible cultural heritage to foster practical skills and create flexible employment,opening a sustainable path to prosperity and rural revitalization for peo...Pioneering initiatives in Yunnan Province are leveraging intangible cultural heritage to foster practical skills and create flexible employment,opening a sustainable path to prosperity and rural revitalization for people with disabilities.ACROSS China,provinces and municipalities have adopted a range of initiatives,based on local conditions to facilitate employment for people with disabilities.In southwest China’s Yunnan Province,home to many ethnic minorities,efforts have focused on integrating employment for disabled people with rural revitalization.展开更多
The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding metho...The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.展开更多
This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and rad...This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and radio in Ghanaian African languages,the book argues for a decolonial politics and praxis in co-creating knowledge with Indigenous communities.It critically links the struggles of global majority countries,showing how colonialism and imperialism obstruct liberatory futures,while foregrounding African language media as vital tools for cultural and epistemological decolonisation.Heralded as part of an unstoppable shift away from Eurocentrism,the book is distinguished by its radical feminist subaltern analysis and its deep grounding in lived experience from Northern Ghana.By excavating local agency within the digital mediascape,Mohammed o!ers a crucial historical and political-economic theorisation of media practice and consumption.展开更多
基金supported by the public welfare program of the Ministry of Environmental Protection of China(No.201509008)the National Natural Science Foundation of China(No.51308527)
文摘The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor（i.e., fine screen room and sludge dewatering house） and two outdoor（i.e., aeration tank and control site） sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria.
基金This work was supported by the National 863 Programm of China "Bioengineering Technique Project 2002A2104101
文摘Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67μg·g-1).
基金Supported by the Natural Science Foundation of China-United Fund(No.U1406402-5)the Postdoctoral Researcher Applied Research Project Funding of Qingdao,China(No.Q51201407)the International Cooperation and Exchanges in Science and Technology(No.2014DFG30890)
文摘The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012.The 16 S rRNA sequences were analyzed from 187 isolated bacterial strains.Three phyla,29 genera and 56 species were identified.The three phyla were Actinobacteria,Firmicutes and Proteobacteria;the Proteobacteria included a-Proteobacteria,P-Proteobacteria and y-Proteobacteria.y-Proteobacteria,Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species.Gram-positive bacteria(Actinobacteria and Firmicutes) accounted for 57.8% of all types identified.There were nine dominant genera,including Curtobacterium,Staphylococcus,and Halomonas,and 14 dominant species including Curtobacterium flaccumfaciens,Curtobacterium pusillum,and Staphylococcus sciuri.Of the strains identified,87.2% were catalase positive or weakly positive.
基金financially supported by the National Nature Science Foundation of China(41771117 and 41877540)the China National Key Research and Development Plan(2017FY100200)+1 种基金the Second Tibetan Plateau Scientific Expedition and Research program(2019QZKK0305)the Key Research and Development project of Zhejiang Province(2018C02031)
文摘Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixed dune and sandy grassland were selected to investigate the soil fungal diversity using a traditional culture-dependent approach.ITS sequencing was applied to identify the fungal strains.The counts of culturable fungi increased significantly from mobile dune to sandy grassland along the gradient of sandy land restoration.The Shannon-Wiener,Simpson and Evenness indices of culturable fungi ranged from 1.26-1.71,0.22-0.37 and 0.83-0.87,respectively.A total of 27 fungal strains were isolated using dilution plate cultural technique.The 27 fungal isolates were clustered into three groups:Ascomycota,Basidiomycota and Mucoromycota at phylum level,indicating that Ascomycota was the dominant fungal phylum(88.9%of the total).The isolated fungi were grouped into 3 phyla,5 classes,6 orders,11 families and 13 genera.The results show that culturable fungi were diverse in sandy land soils and fungal isolates have potential function in lipid turnover,cellulose degradation and ethanol,glucose and fatty acid production.Future studies should be carried out to explore their ecological and biological function for degraded sandy land restoration.
基金financially supported by the National Natural Science Foundation of China(31870479,31570498,and 41801045)the Key Foreign Cooperation Projects of the Bureau of International Cooperation of Chinese Academy of Sciences(131B62KYSB20160014)
文摘Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation.This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils,and the interaction between bacteria and environmental factors.The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis.The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g,respectively,indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil.A total of 98 species belonging to 34 genera were identified,among which Arthrobacter,Bacillus,and Streptomyces were found dominantly and widely distributed.The community of culturable bacteria had obvious sample specificity,and the diversity in hypolithic soil was higher than that in peripheral soil.On the regional scale,the distribution of culturable bacteria and the environmental factors showed regular changes.On the local scale,the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.
基金Supported by the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202151).
文摘[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable bacteria present in the leaves,roots,and rhizosphere soil of both healthy areca palms and those exhibiting symptoms of YLD within the same orchard were conducted.Furthermore,a differential analysis of the bacterial community structure was conducted.[Results]The bacterial count was observed to be greater in healthy areca palm samples compared to those exhibiting disease symptoms.Furthermore,the diversity of bacterial species in healthy areca palm samples surpassed that found in diseased samples.Notably,the bacterial genera that exhibited significant differences between healthy and diseased areca palms included Bacillus velezensis,Paenibacillus validus,Alcaligenes faecalis,Burkholderia territorii,Pseudomonas aeruginosa,Bacillus amyloliquefaciens,etc.[Conclusions]This study may offer data support and technical guidance for the effective prevention and control of YLD in areca palm in the future.
基金supported by the Natural Science Foundation of Jiangsu Province.(BK20242077)。
文摘Next-generation sequencing technologies have significantly advanced our comprehension of microbial diversity and ecological roles within fermented foods.However,culture-based approaches remain essential for a comprehensive understanding of these complex ecosystems.This study integrated culture-dependent and culture-independent techniques to elucidate the microbial diversity and flavor-forming potential of fungi in high-temperature Daqu,a critical solid-state fermentation process in Maotai-flavor Baijiu production.Through iterative cultivation strategies,we successfully isolated and identified 660 pure eukaryotic colonies,representing 33 genera and 58 species,from Daqu samples.This approach significantly improved cultivation efficiency from 20.7 to 63.2%.In addition,despite the optimization of ITS rRNA primer sets to enhance the detection of eukaryotic microorganisms,we still identified 21 genera(comprising 27 species)that were culturable but not detected by high-throughput sequencing analysis.Fermentation experiments demonstrated the robust growth and substantial ethyl acetate production potential of these fungal species,particularly Kluyveromyces marxianus,which exhibited exceptional performance at elevated temperatures(45℃).This study advanced culture-dependent techniques to improve the isolation of eukaryotic microorganisms from Daqu.These findings highlight the importance of culture-based approaches in characterizing microbial diversity and function.
文摘To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including tradi- tional culturing, rDNA internal transcribed spacer （ITS） sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shmblands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance （S）, Shannon-Wiener index （H）, Simpson dominance index （D）, and Pielou evenness index （3） of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66-2.71, 0.92, 0.95~）.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.
文摘At dawn in Wufu Village,in Dinghu District of Zhaoqing City,Guangdong Province,the newly restored Chaoyangli Cultural Retreat is already welcoming its first visitors.A visitor surnamed Chen,who has travelled from Guangzhou with his child,stops in front of the Chaxi Academy to admire local intangible cultural heritage crafts.
文摘China’s booming performing arts sector is reshaping cultural experiences and creating vibrant new spaces for leisure,tourism and creativity The year 2025 marked a period of vigorous expansion for China’s performing arts sector,as the industry evolved from a traditional entertainment field into a comprehensive engine of cultural consumption.
文摘University students,fashion models,street musicians,recent graduates…hundreds of aspiring singers gathered on the campus of the University of Nairobi in Kenya for a music audition.Some had guitars slung across their backs,others drummed rhythms on hand drums,and a few performed melodies they had written on their phones.
文摘In the year 2026,the world cultural exchanges will continue to develop,while the international public opinion environment remainsdive rseand complex.Looking back at this pivotal moment in history,the life journey of Sunny Wu,Chairman of the Hong Kong Doyens’Club,resonates perfectly with the national development.
文摘A Tibetan art form bridges the past and present and connects cultures around the world.THANGKA,a unique form of Tibetan sacred painting,is gaining prominence globally due to its vibrant colors,exquisite craftsmanship,and profound religious and cultural significance.With the acceleration of globalization,this symbol of Tibetan culture that combines artistic expression with spirituality has become a bridge for cultural exchange between the East and the West.Recently,China Today spoke to Yixi Puncog,art collector and council member of the China Association for Preservation and Development of Tibetan Culture,to learn more about Thangka art,its role in international exchange,and how it is enhancing China’s cultural soft power.
文摘This paper divides the centuries-long communication of traditional Chinese medicine(TCM)in France into three progressive stages:the first stage(17th to 19th centuries),characterized by academic recognition and knowledge transmission;the second stage(mid-19th century to late 20th century),focusing on clinical verification and school-based innovation;and the third stage(late 20th century to the present),marked by cultural integration and pluralistic coexistence within systems.The introduction of TCM in France is a process of cross-cultural adaptation and coexistence driven by specific health needs in French society,such as chronic disease management and complementary therapies.The French medical community and sociocultural context have not only verified and interpreted TCM through an empirical scientific perspective,but also realized creative localization by developing indigenous schools such as electroacupuncture and auricular acupuncture.Eventually,through institutional measures including national certification,terminology standardization,and networked healthcare services,TCM has been deeply integrated into France’s medical system and social context,forming a stable ecosystem of cross-cultural adaptation and pluralistic coexistence.The French experience,which takes aligning with local health needs as the starting point,stimulating localized cultural innovation as the core,and promoting systematic integration into local systems as the support,presents a picture of traditional medicine serving modern society,the improvement of global health,as well as mutual learning and symbiosis among different civilizations.
文摘At the Ningxia Museum,visitors can dive into the history of the mysterious Western Xia Dynasty,as well as cultural and ethnic exchanges that occured along the ancient Silk Road centuries ago.THE area of present-day Ningxia Hui Autonomous Region in northwest China was a vital corridor of the ancient Silk Road.Around a thousand years ago,Yuan Hao,leader of the Tangut people,established the Western Xia(also known as Xixia)Dynasty(1038-1227)on this land,which coexisted with the Song,Liao,and Jin dynasties across China for nearly 200 years.
基金Philosophy and Social Sciences Research Project of Shandong Higher Education Institutions:“Research on the Double Helix Mechanism of Yellow River Culture Empowering Ideological and Political Education in Universities from the Perspective of Cultural Confidence Cultivation”(2025ZSYB077)Youth Key Project of Shandong Humanities and Social Sciences Research Project,“Research on Integrating Yellow River Culture into the Cultivation of University Students’Cultural Confidence”Shandong Higher Education Institutions Young Innovation Team Program:“Yellow River Delta Ecological Protection and Governance Innovation Team”(2023RW036).
文摘Guided by the significant theoretical principle of the“Two Integrations”and grounded in Marxist cultural theory as its methodological basis,this paper constructs a bidirectional interpretative model linking“Yellow River Culture”with“Cultural Confidence”.It proposes an integrated“Objective-Content-Path-Support”framework.Through the synergy of three-dimensional objectives,adaptation of stratified content,innovation in four-dimensional pathways,and support from a three-dimensional guarantee system,this framework establishes a closed-loop operational mechanism of“Curriculum-Practice-Evaluation-Feedback”.The study focuses on core issues in integrating Yellow River culture into university education practices,such as content construction,methodological pathways,and institutional guarantees.It aims to provide a systematic reference for universities to fulfill their fundamental task of“fostering virtue and cultivating talent”and to serve the national strategies for ecological protection and high-quality development in the Yellow River Basin.
文摘Your comments are welcome!Please feel free to contact us with your personal stories or vivid pictures!Address:24 Baiwanzhuang St.,Xicheng Dist.,Beijing,100037 E-mail:chinatodaynews@aliyun.com.As Cyprus’newly-appointed Ambassador to China,I have been deeply impressed since my arrival in Beijing last August by the warmth of the Chinese people,the country’s rich heritage of culture and history,and the dynamism of its young innovators.At various events,I have witnessed their relentless drive for self-improvement through innovation,which is a testament to China’s strong emphasis on education,technology,and progress.
文摘Pioneering initiatives in Yunnan Province are leveraging intangible cultural heritage to foster practical skills and create flexible employment,opening a sustainable path to prosperity and rural revitalization for people with disabilities.ACROSS China,provinces and municipalities have adopted a range of initiatives,based on local conditions to facilitate employment for people with disabilities.In southwest China’s Yunnan Province,home to many ethnic minorities,efforts have focused on integrating employment for disabled people with rural revitalization.
基金supported by the Science and Technology Innovation Program of Fujian Agriculture and Forestry University,China(Grant No.KFB22045)the General Program of Natural Science Foundation Fujian,China(Grant No.2023J01460).
文摘The doubled haploid(DH)technique accelerates homozygosity by inducing chromosome doubling in haploid embryos derived from hybrid plants.This approach offers significant advantages over conventional rice breeding methods by shortening the breeding cycle and enabling rapid development of pure homozygous lines.Anther culture(AC)has been established as an efficient and successful method for producing DH plants via androgenesis in rice.However,despite its success in japonica rice.
文摘This work examines media through a decolonial lens,centring Dagba$epistemologies to demonstrate how African languages,cultures,and traditions can transform how we think of knowledge.Focusing on film,television and radio in Ghanaian African languages,the book argues for a decolonial politics and praxis in co-creating knowledge with Indigenous communities.It critically links the struggles of global majority countries,showing how colonialism and imperialism obstruct liberatory futures,while foregrounding African language media as vital tools for cultural and epistemological decolonisation.Heralded as part of an unstoppable shift away from Eurocentrism,the book is distinguished by its radical feminist subaltern analysis and its deep grounding in lived experience from Northern Ghana.By excavating local agency within the digital mediascape,Mohammed o!ers a crucial historical and political-economic theorisation of media practice and consumption.
