[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable ...[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable bacteria present in the leaves,roots,and rhizosphere soil of both healthy areca palms and those exhibiting symptoms of YLD within the same orchard were conducted.Furthermore,a differential analysis of the bacterial community structure was conducted.[Results]The bacterial count was observed to be greater in healthy areca palm samples compared to those exhibiting disease symptoms.Furthermore,the diversity of bacterial species in healthy areca palm samples surpassed that found in diseased samples.Notably,the bacterial genera that exhibited significant differences between healthy and diseased areca palms included Bacillus velezensis,Paenibacillus validus,Alcaligenes faecalis,Burkholderia territorii,Pseudomonas aeruginosa,Bacillus amyloliquefaciens,etc.[Conclusions]This study may offer data support and technical guidance for the effective prevention and control of YLD in areca palm in the future.展开更多
The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoo...The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor（i.e., fine screen room and sludge dewatering house） and two outdoor（i.e., aeration tank and control site） sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria.展开更多
Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes a...Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67μg·g-1).展开更多
The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of ...The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012.The 16 S rRNA sequences were analyzed from 187 isolated bacterial strains.Three phyla,29 genera and 56 species were identified.The three phyla were Actinobacteria,Firmicutes and Proteobacteria;the Proteobacteria included a-Proteobacteria,P-Proteobacteria and y-Proteobacteria.y-Proteobacteria,Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species.Gram-positive bacteria(Actinobacteria and Firmicutes) accounted for 57.8% of all types identified.There were nine dominant genera,including Curtobacterium,Staphylococcus,and Halomonas,and 14 dominant species including Curtobacterium flaccumfaciens,Curtobacterium pusillum,and Staphylococcus sciuri.Of the strains identified,87.2% were catalase positive or weakly positive.展开更多
Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixe...Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixed dune and sandy grassland were selected to investigate the soil fungal diversity using a traditional culture-dependent approach.ITS sequencing was applied to identify the fungal strains.The counts of culturable fungi increased significantly from mobile dune to sandy grassland along the gradient of sandy land restoration.The Shannon-Wiener,Simpson and Evenness indices of culturable fungi ranged from 1.26-1.71,0.22-0.37 and 0.83-0.87,respectively.A total of 27 fungal strains were isolated using dilution plate cultural technique.The 27 fungal isolates were clustered into three groups:Ascomycota,Basidiomycota and Mucoromycota at phylum level,indicating that Ascomycota was the dominant fungal phylum(88.9%of the total).The isolated fungi were grouped into 3 phyla,5 classes,6 orders,11 families and 13 genera.The results show that culturable fungi were diverse in sandy land soils and fungal isolates have potential function in lipid turnover,cellulose degradation and ethanol,glucose and fatty acid production.Future studies should be carried out to explore their ecological and biological function for degraded sandy land restoration.展开更多
Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem pro...Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation.This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils,and the interaction between bacteria and environmental factors.The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis.The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g,respectively,indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil.A total of 98 species belonging to 34 genera were identified,among which Arthrobacter,Bacillus,and Streptomyces were found dominantly and widely distributed.The community of culturable bacteria had obvious sample specificity,and the diversity in hypolithic soil was higher than that in peripheral soil.On the regional scale,the distribution of culturable bacteria and the environmental factors showed regular changes.On the local scale,the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.展开更多
Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Ou...Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Our collaborative team has developed a novel,efficient,and low-toxicity fungicide named Y17991(N-(2-(2,4-bis-(trifluoromethyl)phenoxy)phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide).Preliminary laboratory tests confirmed the significant inhibitory effect of this agent on R.cerealis.Large-area field trials also demonstrated its efficacy,with a disease prevention index of 83.52%,which is 1.97%greater than that of the widely used thifluzamide,and it significantly increased the wheat yield.Moreover,this study explored the impacts of Y17991 on the structure and function of the microbial community in wheat rhizosphere soil.Bacterial communities were more strongly affected than fungal communities.Y17991 significantly modulated key amino acid metabolic pathways and certain biosynthetic processes in diseased wheat rhizospheres,and it also enhanced certain biosynthetic pathways and metabolic activities in healthy wheat rhizospheres.Additionally,the application of Y17991 regulated rhizosphere metabolites,thus exerting significant control over the microbial community.We identified 15 microbial strains potentially involved in the prevention and treatment of R.cerealis,and Y17991 treatment promoted the growth of Pedobacter and Bacillus strains.These strains not only aid in plant growth but they also have the potential for disease prevention.In summary,Y17991 application at a reasonable dose does not cause significant disruption to nontarget rhizosphere microbial communities.In future studies,we will continue to investigate the impacts of Y17991 on nonmicrobial components in soil ecosystems,such as protozoa and nematodes.Our research provides a theoretical basis for the scientific application and promotion of new fungicides and offers a significant reference for establishing a comprehensive system for assessing the ecological impact of pesticides on the environment.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is the most common subtype of primary liver cancer with varied incidence and epidemiology worldwide.Sorafenib is still a recommended treatment for a large proportion of patients...BACKGROUND Hepatocellular carcinoma(HCC)is the most common subtype of primary liver cancer with varied incidence and epidemiology worldwide.Sorafenib is still a recommended treatment for a large proportion of patients with advanced HCC.Different patterns of treatment responsiveness have been identified in differentiated hepatoblastoma HepG2 cells and metastatic HCC SNU449 cells.AIM To define the long non-codingRNA-microRNA-mRNA(lncRNA-miRNA-mRNA)predicted signatures related to selected hallmarks of cancer(apoptosis,autophagy,cell stress,cell dedifferentiation and invasiveness)in RNAseq studies using Sorafenib-treated HepG2 and SNU449 cells.Various available software analyses allowed us to establish the lncRNA-miRNA-mRNA regulatory axes following treatment in HepG2 and SNU449 cells.METHODS HepG2 and SNU449 cells were treated with Sorafenib(10μmol/L)for 24 hours.Total RNA,including small and long RNA,was extracted with a commercial miRNeasy kit.RNAseq was carried out for the identification of changes in lncRNA-miRNA-mRNA regulatory axes.RESULTS MALAT,THAP9-AS1 and SNGH17 appeared to coordinately regulate miR-374b-3p and miR-769-5p that led to upregulation of SMAD7,TIRARP,TFAP4 and FAXDC2 in HepG2 cells.SNHG12,EPB41 L4A-AS1,LINC01578,SNHG12 and GAS5 interacted with let-7b-3p,miR-195-5p and VEGFA in SNU449 cells.The axes MALAT1/hsamir-374b-3p/SMAD7 and MALAT1/hsa-mir-769-5p/TFAP4 were of high relevance for Sorafenib response in HepG2 cells,whereas PVT1/hsa-miR-195-5p/VEGFA was responsible for the differential response of SNU449 cells to Sorafenib treatment.CONCLUSION Critical lncRNAs acting as sponges of miRNA were identified that regulated mRNA expression,whose proteins mainly increased the antitumor effectiveness of the treatment(SMAD7,TIRARP,TFAP4,FAXDC2 and ADRB2).However,the broad regulatory axis leading to increased VEGFA expression may be related to the side effect of Sorafenib in SNU449 cells.展开更多
The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and contin...The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.展开更多
To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were se...To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including tradi- tional culturing, rDNA internal transcribed spacer （ITS） sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shmblands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance （S）, Shannon-Wiener index （H）, Simpson dominance index （D）, and Pielou evenness index （3） of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66-2.71, 0.92, 0.95~）.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.展开更多
本文基于国际文化经济学期刊Journal of Cultural Economics 2007—2023年所发表的335篇文献,运用Citespace软件进行文献计量与可视化分析,揭示国际文化经济学研究的热点演进与理论特征。研究发现,国际文化经济学聚焦文化市场定价机制...本文基于国际文化经济学期刊Journal of Cultural Economics 2007—2023年所发表的335篇文献,运用Citespace软件进行文献计量与可视化分析,揭示国际文化经济学研究的热点演进与理论特征。研究发现,国际文化经济学聚焦文化市场定价机制、文化消费行为、文化产业创新及文化参与模式等微观实证领域,形成以新古典经济学为基础的研究范式。相比之下,国内文化经济学研究受制度经济学和幸福经济学影响,更侧重宏观层面的文化与经济关系探讨,强调文化政策对经济发展的形塑作用。通过比较分析,本文认为国内文化经济学研究需加强与国际理论界的对话,在实现多元、多尺度研究方法融合基础上,开展面向中国式现代化的文化经济实践,实现理论路径突破。未来研究应聚焦三大方向:一是深化文化与经济互动的制度分析,深化“双效统一”的机制研究;二是探索数字技术驱动的文化经济转型,分析区块链、人工智能等最新技术对文化生产、传播与消费的重构路径;三是立足中国式现代化建设实践,构建文化赋能社会经济发展的内生驱动模式,为全球文化经济发展和治理贡献中国智慧。展开更多
In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional c...In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional culture into rural public spaces.Field research has been conducted to assess the current utilization of regional culture in the public spaces of Xiaomaopoying Village.Subsequently,design optimization strategies are analyzed and proposed.Additionally,the practical application of regional cultural elements is examined,in order to offer guidance for the application of cultural creation of traditional characteristic villages in rural public spaces.展开更多
BACKGROUND Hepatic organoid-based modelling,through the elucidation of a range of in vivo biological processes and the recreation of the intricate liver microenvironment,is yielding groundbreaking insights into the pa...BACKGROUND Hepatic organoid-based modelling,through the elucidation of a range of in vivo biological processes and the recreation of the intricate liver microenvironment,is yielding groundbreaking insights into the pathophysiology and personalized medicine approaches for liver diseases.AIM This study was designed to analyse the global scientific output of hepatic organoid research and assess current achievements and future trends through bibliometric analysis.METHODS Articles were retrieved from the Web of Science Core Collection,and CiteSpace 6.3.R1 was employed to analyse the literature,including outputs,journals,and countries,among others.RESULTS Between 2010 and 2024,a total of 991 articles pertaining to hepatic organoid research were published.The journal Hepatology published the greatest number of papers,and journals with an impact factor greater than 10 constituted 60%of the top 10 journals.The United States and Utrecht University were identified as the most prolific country and institution,respectively.Clevers H emerged as the most prolific author,whereas Huch M had the highest number of cocitations,suggesting that both are ideal candidates for academic collaboration.Research on hepatic organoids has exhibited a progressive shift in focus,evolving from initial investigations into model building,differentiation research in stem cells,bile ducts,and progenitor cells,to a broader spectrum encompassing lipid metabolism,single-cell RNA sequencing,and therapeutic applications.The phrases exhibiting citation bursts from 2022 to 2024 include“drug resistance”,“disease model”,and“patient-derived tumor organoids”.CONCLUSION Research on hepatic organoids has increased over the past decade and is expected to continue to grow.Key research areas include applications for liver diseases and drug development.Future trends likely to gain focus include patient-derived tumour organoids,disease modelling,and personalized medicine.展开更多
This study focuses on the deepening paths of ideological and political construction of professional courses in higher vocational colleges,taking the layout design course of the digital media technology major as the re...This study focuses on the deepening paths of ideological and political construction of professional courses in higher vocational colleges,taking the layout design course of the digital media technology major as the research object,and explores the integration mechanism of excellent traditional Chinese culture and professional teaching.The curriculum’s ideological and political reform is advanced through three major measures:Reconstructing the role of teachers,innovating teaching methods,and building a cultural infiltration system.Combined with the three-dimensional assessment mechanism of“professional+culture+value”and the transformation of teaching achievements into competitions,it breaks through the limitations of traditional skill training and systematically solves the problems of formalization tendency and insufficient value guidance existing in the practice of curriculum ideology and politics.It responds to the era proposition of“promoting the creative transformation of excellent traditional Chinese culture,”and provides an operable solution for higher vocational colleges to break the predicament of“emphasizing skills while neglecting culture.”展开更多
The historicity of China's first state-level government(the Xia Dynasty),during which a Great Flood is claimed to have swept through the core of northern China,remains a well-known yet unresolved issue.Archaeologi...The historicity of China's first state-level government(the Xia Dynasty),during which a Great Flood is claimed to have swept through the core of northern China,remains a well-known yet unresolved issue.Archaeologists hypothesize a connection between the legendary events of the Xia Dynasty and archaeological discoveries in the Central China Plains cultural area,encompassing late Neolithic and Bronze Age cultures such as Henan's Longshan,Xinzhai,Erlitou,and Erligang.The authenticity of these speculations has been challenging to substantiate due to the lack of systematic evidence for the Great Flood in the middle to lower Yellow River(YR)Basin.In this paper,we present high-resolution hydrological environmental proxy data,sedimentological remains,and paleontological evidence from the central North China Plain.Our findings provide isochronous evidence of the termination of a hundred-year-long flood period dated to approximately 2080±216 BC,consistent with the observations from lower YR flood plain and marginal marine sediments.These findings both spatially and temporally overlap with the framework of the Great Flood described in the Chinese classics.The alignment between the geoscientific and archaeological evidence and the information in the Chinese classics provides robust confirmation that the Great Flood occurred in the middle to lower YR region during the late Longshan era.展开更多
Brain organoids encompass a large collection of in vitro stem cell–derived 3D culture systems that aim to recapitulate multiple aspects of in vivo brain development and function.First,this review provides a brief int...Brain organoids encompass a large collection of in vitro stem cell–derived 3D culture systems that aim to recapitulate multiple aspects of in vivo brain development and function.First,this review provides a brief introduction to the current state-of-the-art for neuroectoderm brain organoid development,emphasizing their biggest advantages in comparison with classical two-dimensional cell cultures and animal models.However,despite their usefulness for developmental studies,a major limitation for most brain organoid models is the absence of contributing cell types from endodermal and mesodermal origin.As such,current research is highly investing towards the incorporation of a functional vasculature and the microglial immune component.In this review,we will specifically focus on the development of immune-competent brain organoids.By summarizing the different approaches applied to incorporate microglia,it is highlighted that immune-competent brain organoids are not only important for studying neuronal network formation,but also offer a clear future as a new tool to study inflammatory responses in vitro in 3D in a brainlike environment.Therefore,our main focus here is to provide a comprehensive overview of assays to measure microglial phenotype and function within brain organoids,with an outlook on how these findings could better understand neuronal network development or restoration,as well as the influence of physical stress on microglia-containing brain organoids.Finally,we would like to stress that even though the development of immune-competent brain organoids has largely evolved over the past decade,their full potential as a pre-clinical tool to study novel therapeutic approaches to halt or reduce inflammation-mediated neurodegeneration still needs to be explored and validated.展开更多
CLIL,which stands for Content and Language Integrated Learning,is an instructional approach that gives ample curricular and pedagogical attention to content and language outcomes in multilingual educational settings.I...CLIL,which stands for Content and Language Integrated Learning,is an instructional approach that gives ample curricular and pedagogical attention to content and language outcomes in multilingual educational settings.Increasingly,it is heralded as a way to responsibly enact top-down English-Medium-of-Instruction(EMI)policies at the university level,where teachers and students are tasked with developing their English proficiency while remaining competitive in the international job market.However,teachers and teacher educators hoping to implement this approach in their science,technology,engineering and mathematics(STEM)content courses face significant challenges.This article serves as an introduction to a vip-edited special issue that reports on several aspects related to a project of international collaboration called Project SCILLA,an acronym for“STEM Content Integrated with Language-Learning Activities”.We first provide a brief overview of the project,which was developed and carried out in collaboration between Michigan State University and a consortium of 10 rural universities in Kazakhstan as a way to support STEM educators who wish to adapt their teaching practices to Kazakhstan’s Ministry of Education.We then offer an overview of the six articles that comprise the special issue,and call for deliberate and dialogic international collaboration as a way to support teachers responding to language policy demands.展开更多
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ...Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.展开更多
Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease.The accumulation of amyloid-βpeptides,a key hallmark of Alzheimer's disease,is be...Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease.The accumulation of amyloid-βpeptides,a key hallmark of Alzheimer's disease,is believed to induce neuritic abnormalities,including reduced growth,extension,and abnormal growth cone morphology,all of which contribute to decreased connectivity.However,the precise cellular and molecular mechanisms governing this response remain unknown.In this study,we used an innovative approach to demonstrate the effect of amyloid-βon neurite dynamics in both two-dimensional and three-dimensional cultu re systems,in order to provide more physiologically relevant culture geometry.We utilized various methodologies,including the addition of exogenous amyloid-βpeptides to the culture medium,growth substrate coating,and the utilization of human-induced pluripotent stem cell technology,to investigate the effect of endogenous amyloid-βsecretion on neurite outgrowth,thus paving the way for potential future applications in personalized medicine.Additionally,we also explore the involvement of the Nogo signaling cascade in amyloid-β-induced neurite inhibition.We demonstrate that inhibition of downstream ROCK and RhoA components of the Nogo signaling pathway,achieved through modulation with Y-27632(a ROCK inhibitor)and Ibuprofen(a Rho A inhibitor),respectively,can restore and even enhance neuronal connectivity in the presence of amyloid-β.In summary,this study not only presents a novel culture approach that offers insights into the biological process of neurite growth and inhibition,but also proposes a specific mechanism for reduced neural connectivity in the presence of amyloid-βpeptides,along with potential intervention points to restore neurite growth.Thereby,we aim to establish a culture system that has the potential to serve as an assay for measuring preclinical,predictive outcomes of drugs and their ability to promote neurite outgrowth,both generally and in a patient-specific manner.展开更多
Theoretical Studies in Literature and Art(ISSN 0257-0254)is a most highly-recognized peer-reviewed journal in China,and has been listed in Scopus and Ebsco.TSLA,launched in 1980 bimonthly,publishes original articles i...Theoretical Studies in Literature and Art(ISSN 0257-0254)is a most highly-recognized peer-reviewed journal in China,and has been listed in Scopus and Ebsco.TSLA,launched in 1980 bimonthly,publishes original articles in Chinese or English in arts and humanities,especially literary studies.We cover any topic/issue involving,of.about or on but not confined to China in the fields of literary theory,critical theory,aesthetics,philosophy of art,cultural studies,etc.展开更多
基金Supported by the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202151).
文摘[Objectives]The paper was to analyze the structure of the culturable bacterial communities in healthy areca palms and those affected by yellow leaf disease(YLD).[Methods]The quantification and isolation of culturable bacteria present in the leaves,roots,and rhizosphere soil of both healthy areca palms and those exhibiting symptoms of YLD within the same orchard were conducted.Furthermore,a differential analysis of the bacterial community structure was conducted.[Results]The bacterial count was observed to be greater in healthy areca palm samples compared to those exhibiting disease symptoms.Furthermore,the diversity of bacterial species in healthy areca palm samples surpassed that found in diseased samples.Notably,the bacterial genera that exhibited significant differences between healthy and diseased areca palms included Bacillus velezensis,Paenibacillus validus,Alcaligenes faecalis,Burkholderia territorii,Pseudomonas aeruginosa,Bacillus amyloliquefaciens,etc.[Conclusions]This study may offer data support and technical guidance for the effective prevention and control of YLD in areca palm in the future.
基金supported by the public welfare program of the Ministry of Environmental Protection of China(No.201509008)the National Natural Science Foundation of China(No.51308527)
文摘The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor（i.e., fine screen room and sludge dewatering house） and two outdoor（i.e., aeration tank and control site） sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria.
基金This work was supported by the National 863 Programm of China "Bioengineering Technique Project 2002A2104101
文摘Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67μg·g-1).
基金Supported by the Natural Science Foundation of China-United Fund(No.U1406402-5)the Postdoctoral Researcher Applied Research Project Funding of Qingdao,China(No.Q51201407)the International Cooperation and Exchanges in Science and Technology(No.2014DFG30890)
文摘The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America.Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012.The 16 S rRNA sequences were analyzed from 187 isolated bacterial strains.Three phyla,29 genera and 56 species were identified.The three phyla were Actinobacteria,Firmicutes and Proteobacteria;the Proteobacteria included a-Proteobacteria,P-Proteobacteria and y-Proteobacteria.y-Proteobacteria,Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species.Gram-positive bacteria(Actinobacteria and Firmicutes) accounted for 57.8% of all types identified.There were nine dominant genera,including Curtobacterium,Staphylococcus,and Halomonas,and 14 dominant species including Curtobacterium flaccumfaciens,Curtobacterium pusillum,and Staphylococcus sciuri.Of the strains identified,87.2% were catalase positive or weakly positive.
基金financially supported by the National Nature Science Foundation of China(41771117 and 41877540)the China National Key Research and Development Plan(2017FY100200)+1 种基金the Second Tibetan Plateau Scientific Expedition and Research program(2019QZKK0305)the Key Research and Development project of Zhejiang Province(2018C02031)
文摘Soil fungi play a key role in soil functional performance and ecological restoration.To understand the diversity and composition of culturable fungi in soils of Horqin Sandy Land,China,mobile dune,semi-fixed dune,fixed dune and sandy grassland were selected to investigate the soil fungal diversity using a traditional culture-dependent approach.ITS sequencing was applied to identify the fungal strains.The counts of culturable fungi increased significantly from mobile dune to sandy grassland along the gradient of sandy land restoration.The Shannon-Wiener,Simpson and Evenness indices of culturable fungi ranged from 1.26-1.71,0.22-0.37 and 0.83-0.87,respectively.A total of 27 fungal strains were isolated using dilution plate cultural technique.The 27 fungal isolates were clustered into three groups:Ascomycota,Basidiomycota and Mucoromycota at phylum level,indicating that Ascomycota was the dominant fungal phylum(88.9%of the total).The isolated fungi were grouped into 3 phyla,5 classes,6 orders,11 families and 13 genera.The results show that culturable fungi were diverse in sandy land soils and fungal isolates have potential function in lipid turnover,cellulose degradation and ethanol,glucose and fatty acid production.Future studies should be carried out to explore their ecological and biological function for degraded sandy land restoration.
基金financially supported by the National Natural Science Foundation of China(31870479,31570498,and 41801045)the Key Foreign Cooperation Projects of the Bureau of International Cooperation of Chinese Academy of Sciences(131B62KYSB20160014)
文摘Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation.This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils,and the interaction between bacteria and environmental factors.The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis.The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g,respectively,indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil.A total of 98 species belonging to 34 genera were identified,among which Arthrobacter,Bacillus,and Streptomyces were found dominantly and widely distributed.The community of culturable bacteria had obvious sample specificity,and the diversity in hypolithic soil was higher than that in peripheral soil.On the regional scale,the distribution of culturable bacteria and the environmental factors showed regular changes.On the local scale,the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.
基金supported by the National Natural Science Foundation of China(32270296)the Shenzhen Postdoctoral Scientific Research,China(77000-42100004)+1 种基金the Key Basic Research and Development Program of Hubei Province,China(2020BBA052)the Natural Science Foundation of Guangdong Province,China(2024A1515010498)and the Fundamental Research Funds for the Central Universities,Sun Yat-sen University,China.
文摘Sharp eyespot(Rhizoctonia cerealis)is a widespread soil-borne fungal disease that poses a severe threat to wheat health,and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.Our collaborative team has developed a novel,efficient,and low-toxicity fungicide named Y17991(N-(2-(2,4-bis-(trifluoromethyl)phenoxy)phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide).Preliminary laboratory tests confirmed the significant inhibitory effect of this agent on R.cerealis.Large-area field trials also demonstrated its efficacy,with a disease prevention index of 83.52%,which is 1.97%greater than that of the widely used thifluzamide,and it significantly increased the wheat yield.Moreover,this study explored the impacts of Y17991 on the structure and function of the microbial community in wheat rhizosphere soil.Bacterial communities were more strongly affected than fungal communities.Y17991 significantly modulated key amino acid metabolic pathways and certain biosynthetic processes in diseased wheat rhizospheres,and it also enhanced certain biosynthetic pathways and metabolic activities in healthy wheat rhizospheres.Additionally,the application of Y17991 regulated rhizosphere metabolites,thus exerting significant control over the microbial community.We identified 15 microbial strains potentially involved in the prevention and treatment of R.cerealis,and Y17991 treatment promoted the growth of Pedobacter and Bacillus strains.These strains not only aid in plant growth but they also have the potential for disease prevention.In summary,Y17991 application at a reasonable dose does not cause significant disruption to nontarget rhizosphere microbial communities.In future studies,we will continue to investigate the impacts of Y17991 on nonmicrobial components in soil ecosystems,such as protozoa and nematodes.Our research provides a theoretical basis for the scientific application and promotion of new fungicides and offers a significant reference for establishing a comprehensive system for assessing the ecological impact of pesticides on the environment.
基金Supported by Instituto de Salud Carlos III(ISCiii),No.PI19/01266 and No.PI22/00857Consejería de Salud y Familias(Junta de Andalucía),No.PI-0216-2020 and No.PIP-0215-2020Biomedical Research Network Center for Liver and Digestive Diseases(CIBERehd)founded by the ISCIII and co-financed by European Regional Development Fund“A way to achieve Europe”ERDF.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is the most common subtype of primary liver cancer with varied incidence and epidemiology worldwide.Sorafenib is still a recommended treatment for a large proportion of patients with advanced HCC.Different patterns of treatment responsiveness have been identified in differentiated hepatoblastoma HepG2 cells and metastatic HCC SNU449 cells.AIM To define the long non-codingRNA-microRNA-mRNA(lncRNA-miRNA-mRNA)predicted signatures related to selected hallmarks of cancer(apoptosis,autophagy,cell stress,cell dedifferentiation and invasiveness)in RNAseq studies using Sorafenib-treated HepG2 and SNU449 cells.Various available software analyses allowed us to establish the lncRNA-miRNA-mRNA regulatory axes following treatment in HepG2 and SNU449 cells.METHODS HepG2 and SNU449 cells were treated with Sorafenib(10μmol/L)for 24 hours.Total RNA,including small and long RNA,was extracted with a commercial miRNeasy kit.RNAseq was carried out for the identification of changes in lncRNA-miRNA-mRNA regulatory axes.RESULTS MALAT,THAP9-AS1 and SNGH17 appeared to coordinately regulate miR-374b-3p and miR-769-5p that led to upregulation of SMAD7,TIRARP,TFAP4 and FAXDC2 in HepG2 cells.SNHG12,EPB41 L4A-AS1,LINC01578,SNHG12 and GAS5 interacted with let-7b-3p,miR-195-5p and VEGFA in SNU449 cells.The axes MALAT1/hsamir-374b-3p/SMAD7 and MALAT1/hsa-mir-769-5p/TFAP4 were of high relevance for Sorafenib response in HepG2 cells,whereas PVT1/hsa-miR-195-5p/VEGFA was responsible for the differential response of SNU449 cells to Sorafenib treatment.CONCLUSION Critical lncRNAs acting as sponges of miRNA were identified that regulated mRNA expression,whose proteins mainly increased the antitumor effectiveness of the treatment(SMAD7,TIRARP,TFAP4,FAXDC2 and ADRB2).However,the broad regulatory axis leading to increased VEGFA expression may be related to the side effect of Sorafenib in SNU449 cells.
基金supported by the National Natural Science Foundation of China,Nos.81901156(to ZZ),82271200(to ZZ),82171308(to XC)the Fundamental Research Funds for the Central Universities,No.xzy012022035(to ZZ)+1 种基金the Natural Science Foundation of Shaanxi Province,Nos.2021JM-261(to QK),2023-YBSF-303(to ZZ)Traditional Chinese Medicine Project of Shaanxi Province,No.2019-ZZ-JC047(to QK)。
文摘The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.
文摘To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including tradi- tional culturing, rDNA internal transcribed spacer （ITS） sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shmblands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance （S）, Shannon-Wiener index （H）, Simpson dominance index （D）, and Pielou evenness index （3） of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66-2.71, 0.92, 0.95~）.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.
文摘本文基于国际文化经济学期刊Journal of Cultural Economics 2007—2023年所发表的335篇文献,运用Citespace软件进行文献计量与可视化分析,揭示国际文化经济学研究的热点演进与理论特征。研究发现,国际文化经济学聚焦文化市场定价机制、文化消费行为、文化产业创新及文化参与模式等微观实证领域,形成以新古典经济学为基础的研究范式。相比之下,国内文化经济学研究受制度经济学和幸福经济学影响,更侧重宏观层面的文化与经济关系探讨,强调文化政策对经济发展的形塑作用。通过比较分析,本文认为国内文化经济学研究需加强与国际理论界的对话,在实现多元、多尺度研究方法融合基础上,开展面向中国式现代化的文化经济实践,实现理论路径突破。未来研究应聚焦三大方向:一是深化文化与经济互动的制度分析,深化“双效统一”的机制研究;二是探索数字技术驱动的文化经济转型,分析区块链、人工智能等最新技术对文化生产、传播与消费的重构路径;三是立足中国式现代化建设实践,构建文化赋能社会经济发展的内生驱动模式,为全球文化经济发展和治理贡献中国智慧。
文摘In the context of rural revitalization,the landscape design of public spaces in Enshi Xiaomaopoying Village presents both new opportunities and challenges.This study explores the significance of integrating regional culture into rural public spaces.Field research has been conducted to assess the current utilization of regional culture in the public spaces of Xiaomaopoying Village.Subsequently,design optimization strategies are analyzed and proposed.Additionally,the practical application of regional cultural elements is examined,in order to offer guidance for the application of cultural creation of traditional characteristic villages in rural public spaces.
基金Supported by National Natural Science Foundation of China,No.81630080,No.82305179,and No.82374181The China Postdoctoral Science Foundation Grant,No.2019M650600+1 种基金The Beijing University of Chinese Medicine“Decoding Traditional Chinese Medicine”Project,No.2023-JYB-JBZD-036The Hefei National Research Center for Physical Sciences at the Microscale,No.KF2021104.
文摘BACKGROUND Hepatic organoid-based modelling,through the elucidation of a range of in vivo biological processes and the recreation of the intricate liver microenvironment,is yielding groundbreaking insights into the pathophysiology and personalized medicine approaches for liver diseases.AIM This study was designed to analyse the global scientific output of hepatic organoid research and assess current achievements and future trends through bibliometric analysis.METHODS Articles were retrieved from the Web of Science Core Collection,and CiteSpace 6.3.R1 was employed to analyse the literature,including outputs,journals,and countries,among others.RESULTS Between 2010 and 2024,a total of 991 articles pertaining to hepatic organoid research were published.The journal Hepatology published the greatest number of papers,and journals with an impact factor greater than 10 constituted 60%of the top 10 journals.The United States and Utrecht University were identified as the most prolific country and institution,respectively.Clevers H emerged as the most prolific author,whereas Huch M had the highest number of cocitations,suggesting that both are ideal candidates for academic collaboration.Research on hepatic organoids has exhibited a progressive shift in focus,evolving from initial investigations into model building,differentiation research in stem cells,bile ducts,and progenitor cells,to a broader spectrum encompassing lipid metabolism,single-cell RNA sequencing,and therapeutic applications.The phrases exhibiting citation bursts from 2022 to 2024 include“drug resistance”,“disease model”,and“patient-derived tumor organoids”.CONCLUSION Research on hepatic organoids has increased over the past decade and is expected to continue to grow.Key research areas include applications for liver diseases and drug development.Future trends likely to gain focus include patient-derived tumour organoids,disease modelling,and personalized medicine.
文摘This study focuses on the deepening paths of ideological and political construction of professional courses in higher vocational colleges,taking the layout design course of the digital media technology major as the research object,and explores the integration mechanism of excellent traditional Chinese culture and professional teaching.The curriculum’s ideological and political reform is advanced through three major measures:Reconstructing the role of teachers,innovating teaching methods,and building a cultural infiltration system.Combined with the three-dimensional assessment mechanism of“professional+culture+value”and the transformation of teaching achievements into competitions,it breaks through the limitations of traditional skill training and systematically solves the problems of formalization tendency and insufficient value guidance existing in the practice of curriculum ideology and politics.It responds to the era proposition of“promoting the creative transformation of excellent traditional Chinese culture,”and provides an operable solution for higher vocational colleges to break the predicament of“emphasizing skills while neglecting culture.”
基金Strategic Priority Research Program of the Chinese Academy of Sciences,No.XDB26000000National Natural Science Foundation of China,No.41888101,No.42072209Geological Survey Projects of China,No.DD20189629,No.DD20190370。
文摘The historicity of China's first state-level government(the Xia Dynasty),during which a Great Flood is claimed to have swept through the core of northern China,remains a well-known yet unresolved issue.Archaeologists hypothesize a connection between the legendary events of the Xia Dynasty and archaeological discoveries in the Central China Plains cultural area,encompassing late Neolithic and Bronze Age cultures such as Henan's Longshan,Xinzhai,Erlitou,and Erligang.The authenticity of these speculations has been challenging to substantiate due to the lack of systematic evidence for the Great Flood in the middle to lower Yellow River(YR)Basin.In this paper,we present high-resolution hydrological environmental proxy data,sedimentological remains,and paleontological evidence from the central North China Plain.Our findings provide isochronous evidence of the termination of a hundred-year-long flood period dated to approximately 2080±216 BC,consistent with the observations from lower YR flood plain and marginal marine sediments.These findings both spatially and temporally overlap with the framework of the Great Flood described in the Chinese classics.The alignment between the geoscientific and archaeological evidence and the information in the Chinese classics provides robust confirmation that the Great Flood occurred in the middle to lower YR region during the late Longshan era.
基金funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skodowska-Curie grant agreement No.813263(PMSMat Train,granted to UF,PP,MV,and DP)provided by the Fund for Scientific Research Flanders(FWO-Vlaanderen)of the Flemish Government(FWO sabbatical bench fee K800224N granted to PP)and ERA-NET Re Park(granted to PP)。
文摘Brain organoids encompass a large collection of in vitro stem cell–derived 3D culture systems that aim to recapitulate multiple aspects of in vivo brain development and function.First,this review provides a brief introduction to the current state-of-the-art for neuroectoderm brain organoid development,emphasizing their biggest advantages in comparison with classical two-dimensional cell cultures and animal models.However,despite their usefulness for developmental studies,a major limitation for most brain organoid models is the absence of contributing cell types from endodermal and mesodermal origin.As such,current research is highly investing towards the incorporation of a functional vasculature and the microglial immune component.In this review,we will specifically focus on the development of immune-competent brain organoids.By summarizing the different approaches applied to incorporate microglia,it is highlighted that immune-competent brain organoids are not only important for studying neuronal network formation,but also offer a clear future as a new tool to study inflammatory responses in vitro in 3D in a brainlike environment.Therefore,our main focus here is to provide a comprehensive overview of assays to measure microglial phenotype and function within brain organoids,with an outlook on how these findings could better understand neuronal network development or restoration,as well as the influence of physical stress on microglia-containing brain organoids.Finally,we would like to stress that even though the development of immune-competent brain organoids has largely evolved over the past decade,their full potential as a pre-clinical tool to study novel therapeutic approaches to halt or reduce inflammation-mediated neurodegeneration still needs to be explored and validated.
基金funding from the U.S.-Kazakhstan University Partnerships program funded by the U.S.Mission to Kazakhstan and administered by American Councils[Award number SKZ100-19-CA-0149].
文摘CLIL,which stands for Content and Language Integrated Learning,is an instructional approach that gives ample curricular and pedagogical attention to content and language outcomes in multilingual educational settings.Increasingly,it is heralded as a way to responsibly enact top-down English-Medium-of-Instruction(EMI)policies at the university level,where teachers and students are tasked with developing their English proficiency while remaining competitive in the international job market.However,teachers and teacher educators hoping to implement this approach in their science,technology,engineering and mathematics(STEM)content courses face significant challenges.This article serves as an introduction to a vip-edited special issue that reports on several aspects related to a project of international collaboration called Project SCILLA,an acronym for“STEM Content Integrated with Language-Learning Activities”.We first provide a brief overview of the project,which was developed and carried out in collaboration between Michigan State University and a consortium of 10 rural universities in Kazakhstan as a way to support STEM educators who wish to adapt their teaching practices to Kazakhstan’s Ministry of Education.We then offer an overview of the six articles that comprise the special issue,and call for deliberate and dialogic international collaboration as a way to support teachers responding to language policy demands.
文摘Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.
基金supported by a BBSRC CASE training studentship,No.BB/K011413/1(to KG)。
文摘Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease.The accumulation of amyloid-βpeptides,a key hallmark of Alzheimer's disease,is believed to induce neuritic abnormalities,including reduced growth,extension,and abnormal growth cone morphology,all of which contribute to decreased connectivity.However,the precise cellular and molecular mechanisms governing this response remain unknown.In this study,we used an innovative approach to demonstrate the effect of amyloid-βon neurite dynamics in both two-dimensional and three-dimensional cultu re systems,in order to provide more physiologically relevant culture geometry.We utilized various methodologies,including the addition of exogenous amyloid-βpeptides to the culture medium,growth substrate coating,and the utilization of human-induced pluripotent stem cell technology,to investigate the effect of endogenous amyloid-βsecretion on neurite outgrowth,thus paving the way for potential future applications in personalized medicine.Additionally,we also explore the involvement of the Nogo signaling cascade in amyloid-β-induced neurite inhibition.We demonstrate that inhibition of downstream ROCK and RhoA components of the Nogo signaling pathway,achieved through modulation with Y-27632(a ROCK inhibitor)and Ibuprofen(a Rho A inhibitor),respectively,can restore and even enhance neuronal connectivity in the presence of amyloid-β.In summary,this study not only presents a novel culture approach that offers insights into the biological process of neurite growth and inhibition,but also proposes a specific mechanism for reduced neural connectivity in the presence of amyloid-βpeptides,along with potential intervention points to restore neurite growth.Thereby,we aim to establish a culture system that has the potential to serve as an assay for measuring preclinical,predictive outcomes of drugs and their ability to promote neurite outgrowth,both generally and in a patient-specific manner.
文摘Theoretical Studies in Literature and Art(ISSN 0257-0254)is a most highly-recognized peer-reviewed journal in China,and has been listed in Scopus and Ebsco.TSLA,launched in 1980 bimonthly,publishes original articles in Chinese or English in arts and humanities,especially literary studies.We cover any topic/issue involving,of.about or on but not confined to China in the fields of literary theory,critical theory,aesthetics,philosophy of art,cultural studies,etc.
