A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In th...A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability.展开更多
This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of...This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of highly skilled talents in urban agriculture in the era of smart agriculture and their cultivation pathways and practices.It proposes measures such as building multi-level practical teaching platforms,implementing the“Enjoy Horticulture”series of high-quality activities,and establishing the“1234”applied talent training model to cultivate high-quality talents that meet the development needs of modern urban horticulture industry.Taking Beijing University of Agriculture and other universities as examples,the paper analyzes the practical cases and effects of the urban horticulture discipline’s industry-education-research collaborative talent training model,which has reference significance for further improving and perfecting the urban horticulture industry-education-research collaborative talent training plan.展开更多
To promote the growth of cutting seeding of poplar(Populus L.),nitrogen(N)fertilizer and surface weed managements were required.We here conducted a pot experiment to examine the effects of natural vegetation,barnyardg...To promote the growth of cutting seeding of poplar(Populus L.),nitrogen(N)fertilizer and surface weed managements were required.We here conducted a pot experiment to examine the effects of natural vegetation,barnyardgrass(Echinochloa Beauv.),and sesbania(Sesbania cannabina pers.)on the growth of poplar cutting seedlings,soil properties,and ammonia(NH3)volatilization under three N inputs(0,0.5,and 1.5 g/pot,i.e.,N0,N0.5,and N1,respectively).Results showed that N application promoted the growth of poplar cutting seedlings,including plant height,ground diameter,and biomass,compared with N0 treatment.Moreover,under N0,sesbania significantly increased the plant height by 87.1%,barnyardgrass and sesbania significantly increased the ground diameter(16.2%and 51.5%),and biomass(67.4%and 74.7%)of poplar cutting seedlings,compared with natural vegetation management.Compared to natural vegetation,soil organic matter(SOM)of barnyardgrass and sesbania covered soil significantly increased by 12.4%and 18.7%at N1,respectively.In addition,soil total N(TN)content was significantly increased by 15.8%in barnyardgrass planted at N0.The soil ammonium N(NH_(4)^(+)-N)content decreased with the planting of barnyardgrass and sesbania across all levels of N application.At N0.5,the nitrate N(NO_(3)^(−)-N)content of soil planted with barnyardgrass significantly increased compared to both the natural vegetation and the sesbania groups.Compared to the natural vegetation,the soil available phosphorus(AP)content of the barnyardgrass group significantly increasing by 78.8%at N0.5,soil available potassium(AK)content was significantly reduced by 12.5%in the sesbania group at N0 and increased by 24.1%in the barnyardgrass group at N1.We found that cumulative NH3 emissions were significantly higher in all treatment groups at the N1 level than that at the N0.5 level,while the differences among the three plants treated were not significant.The results suggest that both barnyardgrass and sesbania promote seedling growth in the short term,while also increase certain properties.Therefore,effective herb management during the seedling stage is recommended in nurseries to support seedling growth and retain soil fertility.展开更多
In the context of"high-quality development"construction,the establishment and operational status of the national-level scientific research platforms at provincial universities—specifically,the large-scale i...In the context of"high-quality development"construction,the establishment and operational status of the national-level scientific research platforms at provincial universities—specifically,the large-scale instrument sharing platforms—are critically linked to the production of scientific research outcomes and the development of interdisciplinary talents.This study focuses on the public platform of the State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization,aiming to innovate the management system,establish a technical platform,effectively integrate resources,and actively engage graduate students in the platform management.While ensuring the efficient operation of the large-scale instrument platform at state key laboratories,it offers a novel approach to cultivating graduate students into versatile talents.展开更多
The cultivation of innovative talents has become the primary objective of talent development in colleges and universities and is also essential for the personal development of master's degree students.Ethnic medic...The cultivation of innovative talents has become the primary objective of talent development in colleges and universities and is also essential for the personal development of master's degree students.Ethnic medicine,a key discipline at Guangxi University of Chinese Medicine,aims to cultivate high-level master's degree talents who possess comprehensive qualities,strong innovative capabilities,and distinctive characteristics of Zhuang medicine.This program is designed to better serve the local economic development and social needs of Guangxi,thereby promoting the substantive advancement of higher education within traditional medical institutions.展开更多
THE peony,known as the“king of flowers”in China,is one of the country’s most celebrated flowers.Luoyang,a historically significant city in Henan Province,is widely considered the birthplace of peonies and a center ...THE peony,known as the“king of flowers”in China,is one of the country’s most celebrated flowers.Luoyang,a historically significant city in Henan Province,is widely considered the birthplace of peonies and a center of peony culture,which originated in the city over 1,600 years ago.Today,the city boasts enormous peony varieties,a long cultivation history,and a rich culture surrounding the flower.展开更多
As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production an...As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.展开更多
The Liaohe River Valley was one of the key centers of the origination and development of agriculture in northern China during the Holocene.To understand the long-term interaction among the evolutions of climate,agricu...The Liaohe River Valley was one of the key centers of the origination and development of agriculture in northern China during the Holocene.To understand the long-term interaction among the evolutions of climate,agriculture,and human activities,it is essential to quantitatively reconstruct the spatiotemporal changes in regional prehistoric human land use.In this study,known archaeological sites and a prehistoric land use model(PLUM)were combined to reconstruct changes in land use in the Liaohe River Valley during 8-2 ka BP from a quantitative perspective.The land use area experienced two stages of increase(during 8-5 ka BP and after 4 ka BP)and one stage of decrease(during 5-4 ka BP);these periods were characterized by spatial expansions and contractions.The land use intensity level differed significantly in the western and eastern parts of the valley before 4 ka BP,but the situation changed as the distribution center of the human activity shifted to the southern part of the valley around 5-4 ka BP.Overall,the spatial and temporal changes in the land use areas in both the western and eastern parts of the valley responded well to variations in precipitation during 8-2 ka BP,which potentially provides useful insights into understanding the responses of human activity to future climate change.展开更多
In the context of the new era,deepening education reform and improving the quality of talent cultivation are important measures for universities to align with industry trends and the development needs of students.With...In the context of the new era,deepening education reform and improving the quality of talent cultivation are important measures for universities to align with industry trends and the development needs of students.With the continuous growth of the economy,the construction industry is undergoing rapid development and transformation,and there is an increasing demand for high-quality and high-level applied talents,which poses certain challenges to the architecture majors in universities.Therefore,universities should actively follow the industry development trends and the characteristics of talents,clarify the talent cultivation objectives,optimize the professional teaching system,and promote the high-quality development of education.The cultivation of applied talents in the architecture major of universities is not only an internal requirement for the development of the construction industry but also an important part of the country’s innovation-driven development strategy.It is of great significance for promoting scientific and technological progress,enhancing cultural confidence,and promoting the comprehensive development of the economy and society.展开更多
Significant variations in global temperatures and weather patterns over time are known as climate change.Although it occurs naturally,human activities—particularly the burning of fossil fuels,deforestation,and indust...Significant variations in global temperatures and weather patterns over time are known as climate change.Although it occurs naturally,human activities—particularly the burning of fossil fuels,deforestation,and industrial processes—are accelerating these changes,which have various detrimental effects on the environment.This review aims to highlight the edapho-climatic requirements of this cactus and the advantages and challenges of its cultivation to mitigate climate change.The prickly pear cactus is a plant with numerous financial and environmental advantages.It needs well-draining,sandy or gravelly soil to avoid root rot and do best in full sun.With a strong tolerance for dryness,they thrive in arid or semi-arid regions with scorching summers and prefer sparing watering.Despite being suited to tropical climates,some species can tolerate freezing temperatures and sporadic frost.Once established,these hardy plants require little care and thrive in nutrient-poor soils,which makes them perfect for xeriscaping or challenging growing environments.Because of its high water use efficiency ratio and low water requirements,prickly pear can be grown in marginally dry and semi-arid areas.The cactus does contribute to the ecological and socioeconomic fight against climate change.For instance,it supports sustainable agriculture,biodiversity preservation,soil restoration,carbon sequestration,and effective water usage.Demarcating dry and semi-arid zones and fostering employment in these areas is beneficial from a socioeconomic standpoint.The prickly pear’s traditional cultural heritage supports its current economic function as a crop that can withstand drought.While ecological threats necessitate balanced management,this adaptability promotes sustainable growth.Innovations in bioenergy and value-added goods build on its historical applications,increasing its socioeconomic advantages and,eventually,its worldwide significance.展开更多
Carrots have an important role for a life of the men.Everybody can use carrots in the meals or the drinks,he uses carrots such as the medicaments to cure many diseases,too.Carrots are very necessary,we can tell the fo...Carrots have an important role for a life of the men.Everybody can use carrots in the meals or the drinks,he uses carrots such as the medicaments to cure many diseases,too.Carrots are very necessary,we can tell the following effects:carrots help improve eyesight,especially,carrots contain vitamin A,essential nutrients for health of the eyes.When our bodies have the vitamin A deficiency in the long time,visual cells in the eye retina can been hurt,make dry eyes,cause the visual disturbances and it is serious that everyone does not see.Carrots support to cure the diabetes;help strengthen the immunity;improve the health cardiovascular;help beautiful healthy skin;can reduce the risk of cancer.Besides,everyone drinks carrot juice to stimulate hair growth and stabilize blood pressure.Carrots contain a lot of beta carotene,fiber,vitamin K1,potassium as well as antioxidants and are grown popularly in all regions of Vietnam.The cultivating soil,irrigating water and carrot samples were collected in Loc Thanh village,Bao Lam district,Lam Dong province and some indicators were analyzed.The results showed that the soil sample was poor in nutrient by N,P,K indicators that were lower than the standards;but pH,Al3+and Fe3+were higher than the standards.The water sample did not contain the toxic heavy metals such as:As,Pb,Cd;but the indicators of Cu,Zn,Fe were higher than the standards.For the carrot sample,the indicators of As,Pb,Cu,Zn exceeded the allowable threshold;the contents of lipid and protein were lower than the standards but NO3-was within allowable standards.However,analysis of carrot samples grown by us using organic methods showed that some heavy metal indicators such as:As,Pb,Cu were lower than allowable standards.This showed that it is necessary to choose the type of soil,water and organic method for growing carrots in order to harvest good quality carrots and safety for users.We studied an environment of soil,water and analyzed the carrots to find a nutrition level,nutritional value and the metal accumulation in order to improve a life of men.展开更多
Wheat(Triticum aestivum L.)is a staple crop critical for global food security,yet its productivity is significantly affected by abiotic stresses such as drought,salinity,and waterlogging,which are exacerbated by clima...Wheat(Triticum aestivum L.)is a staple crop critical for global food security,yet its productivity is significantly affected by abiotic stresses such as drought,salinity,and waterlogging,which are exacerbated by climate change.This study evaluated the effects of these stresses on vegetative growth,physiological responses,and yield.Field experiments were conducted using a Randomized Complete Block Design(RCBD)at the Mona Reclamation Experimental Project(MREP),WAPDA,Bhalwal,Sargodha,Punjab Pakistan.Stress treatments included three levels of drought(25%,50%,and 75%field capacity),salinity(4,8,and 12 dS/m),and waterlogging(24,48,and 72 h).Key parameters measured included plant height,leaf area,tiller number,stomatal conductance,chlorophyll content,and antioxidant enzyme activities.The results revealed that drought stress caused a 46%reduction in yield,while salinity and waterlogging reduced yield by 54%and 35%,respectively,with statistically significant differences(p 0.05).Key<physiological changes included a significant reduction in stomatal conductance(from 0.55 to 0.15 mmol m^(2)/s under drought stress,p 0.01)and chlorophyll content(from 48 to 28 SPAD units under drought,p 0.01).Biochemical<<responses indicated elevated levels of malondialdehyde(MDA)and hydrogen peroxide(H2O2),with significant increases in antioxidant enzyme activities,particularly superoxide dismutase(SOD)and catalase(CAT).These findings underscore the need for developing stress-tolerant wheat varieties and implementing agronomic practices to mitigate the impact of abiotic stresses on wheat yield.展开更多
Climate variability significantly impacts agricultural water resources,particularly in regions like Vietnam's Plain of Reeds that heavily utilize rain-fed conditions.This study employs the FAO-AquaCrop model to es...Climate variability significantly impacts agricultural water resources,particularly in regions like Vietnam's Plain of Reeds that heavily utilize rain-fed conditions.This study employs the FAO-AquaCrop model to estimate current and future irrigation water needs for rice cultivation in this critical subregion,aiming to identify optimal sowing schedules(OSS)that enhance rainwater utilization and reduce irrigation dependency.The model was driven by current climate data and future projections(2041-2070 and 2071-2099)derived from downscaled Global Circulation Models under RCP4.5 and RCP8.5 scenarios.The AquaCrop model demonstrated robust performance during validation and calibration,with d-values(0.82-0.93)and R²values(0.85-0.92)indicating strong predictive accuracy for rice yield.Simulation results for efficient irrigation water potential(IWP)under RCP4.5 revealed that strategic shifts in sowing dates can substantially alter water requirements;for instance,advancing the winter-spring sowing to December 5th decreased IWP by 15.6%in the 2041-2070 period,while delaying summer-autumn crop sowing to April 20th increased IWP by 48.6%due to greater reliance on irrigation as rainfall patterns shift.Similar dynamic responses were observed for the 2071-2099 period and for autumn-winter crops.These findings underscore that AquaCrop modeling can effectively predict future irrigation needs and that adjusting cultivation calendars presents a viable,low-cost adaptation strategy.This approach allows farmers in the Plain of Reeds to optimize rainwater use,thereby reducing dependency on supplementary irrigation and mitigating the adverse impacts of climate variability,contributing to more sustainable agricultural water management.展开更多
To address the severe challenges posed by the international situation and meet the needs of the national major development strategies,the traditional software engineering talent cultivation model lacks interdisciplina...To address the severe challenges posed by the international situation and meet the needs of the national major development strategies,the traditional software engineering talent cultivation model lacks interdisciplinary education focused on specific fields,making it difficult to cultivate engineering leaders with multidisciplinary backgrounds who are capable of solving complex real-world problems.To solve this problem,based on the decade-long interdisciplinary talent cultivation achievements of the College of Software Engineering at Sichuan University,this article proposes the“Software Engineering+”innovative talent cultivation paradigm.It provides an analysis through professional construction of interdisciplinary talents,the design of talent cultivation frameworks,the formulation of cultivation plans,the establishment of interdisciplinary curriculum systems,the reform of teaching modes,and the improvement of institutional systems.Scientific solutions are proposed,and five project models implemented and operated by the College of Software Engineering at Sichuan University are listed as practical examples,offering significant reference value.展开更多
Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this wor...Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.展开更多
Rice(Oryza sativa L.)plays a pivotal role in global food security,yet its breeding is constrained by its long generation time and seasonality.To enhance rice breeding efficiency and meet future food demands,we have de...Rice(Oryza sativa L.)plays a pivotal role in global food security,yet its breeding is constrained by its long generation time and seasonality.To enhance rice breeding efficiency and meet future food demands,we have developed a vertical hydroponic breeding system integrated with light-emitting diodes(LEDs)light-ing in a closed plant factory(PF),which significantly accelerates rice growth and generation advance-ment.The results show that indica rice can be harvested as early as after 63 days of cultivation,a 50%reduction compared with field cultivation,enabling the annual harvesting of 5-6 generations within the PF.A hyperspectral imaging(HSI)system and attenuated total reflectance infrared(ATR-IR)spec-troscopy were further employed to characterize the chemical composition of the PF-and field-cultivated rice.Metabolomics analysis with ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and gas chromatography-mass spectrometry(GC-MS)revealed that,com-pared with the field-cultivated rice,the PF-cultivated rice exhibited an up-regulation of total phenolic acids along with 68 non-volatile and 19 volatile metabolites,such as isovitexin,succinic acid,and methylillicinone F.Overall,this study reveals the unique metabolic profile of PF-cultivated rice and high-lights the potential of PFs to accelerate the breeding of crops such as rice,offering an innovative agricul-tural strategy to support food security in the face of global population growth and climate change.展开更多
The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed ...The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed that while employment placement rates for bioengineering graduates remain stable,starting salaries exhibit limited growth and career trajectories lack clarity.These challenges originate from a curriculum lagging behind technological progress,inadequate practical training,and a mismatch between student competencies and industry expectations.To address these issues,this paper proposed a strategic framework grounded in"demand-driven design,industry-education integration,and competence-centered development".Key strategies include dynamic curriculum renewal,collaborative university-industry training,holistic competency development,and personalized student support,which collectively aim at enhancing graduates employment competitiveness and long-term professional sustainability.展开更多
In the context of the new quality productive forces,the advancement of finance and economics institutions relies heavily on the support of engineering disciplines to foster synergies between new engineering and busine...In the context of the new quality productive forces,the advancement of finance and economics institutions relies heavily on the support of engineering disciplines to foster synergies between new engineering and business innovation.Based on the requirements for skilled manpower for the new financial development,we have initiated an initial inquiry into establishing artificial intelligence(AI)major in economic universities under the framework of the new engineering paradigm.From the national policies and the development rules of traditional advantageous majors in universities,we analyze the current challenges and opportunities in the construction of artificial intelligence majors and explore the talent cultivation model of artificial intelligence majors in economic universities under the framework of the integrated development of“New Engineering”and“New Finance”.In conclusion,we propose some viable solutions by giving a case study at Hubei University of Economics.展开更多
Chinese medicine,as a treasure of China’s traditional culture,has a long history and deep cultural heritage,and talents are the first resource for the development of Chinese medicine.In ancient times,there were many ...Chinese medicine,as a treasure of China’s traditional culture,has a long history and deep cultural heritage,and talents are the first resource for the development of Chinese medicine.In ancient times,there were many famous Chinese medicine practitioners with excellent medical skills and fame,and their main ways of success were through teaching and family tradition.The cultivation of high medical ethics,benevolence and love,and helping the world and the people is the source of motivation for success;familiarizing oneself with the classics,understanding the humanities,and being knowledgeable about things past and present is an important way for success;practicing diligently,learning from all the strengths,and observing the right and innovating is the key to success.This paper analyzes the growth path of ancient Chinese medicine masters and discusses the advantages and shortcomings of contemporary Chinese medicine talent training,with a view to providing certain references for the cultivation of Chinese medicine talents.展开更多
Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while bala...Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.展开更多
基金supported by the National High Technology Research and Development Program of China(grant 2007AA09Z434)
文摘A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability.
基金The Enhancement Project of Young Teachers Research Innovation Ability(JKC2022006)Beijing Municipal Higher Education Institutions’Teacher Team Construction Support Plan-High-Level Teaching Innovation Team(BPHR20220211)+1 种基金Beijing Higher Education Undergraduate Teaching Reform and Innovation Project(2023003)2024 Beijing University of Agriculture Student Party Members“Vanguard Force Action”Project。
文摘This paper discusses the development characteristics of urban horticulture under the background of smart agriculture,as well as the application of artificial intelligence technology in it.It analyzes the importance of highly skilled talents in urban agriculture in the era of smart agriculture and their cultivation pathways and practices.It proposes measures such as building multi-level practical teaching platforms,implementing the“Enjoy Horticulture”series of high-quality activities,and establishing the“1234”applied talent training model to cultivate high-quality talents that meet the development needs of modern urban horticulture industry.Taking Beijing University of Agriculture and other universities as examples,the paper analyzes the practical cases and effects of the urban horticulture discipline’s industry-education-research collaborative talent training model,which has reference significance for further improving and perfecting the urban horticulture industry-education-research collaborative talent training plan.
基金funded by the Science and Technology Innovation Programof Jiangsu Province,China for“Carbon Dioxide Emission Peaking and Carbon Neutrality”(BE2022307).
文摘To promote the growth of cutting seeding of poplar(Populus L.),nitrogen(N)fertilizer and surface weed managements were required.We here conducted a pot experiment to examine the effects of natural vegetation,barnyardgrass(Echinochloa Beauv.),and sesbania(Sesbania cannabina pers.)on the growth of poplar cutting seedlings,soil properties,and ammonia(NH3)volatilization under three N inputs(0,0.5,and 1.5 g/pot,i.e.,N0,N0.5,and N1,respectively).Results showed that N application promoted the growth of poplar cutting seedlings,including plant height,ground diameter,and biomass,compared with N0 treatment.Moreover,under N0,sesbania significantly increased the plant height by 87.1%,barnyardgrass and sesbania significantly increased the ground diameter(16.2%and 51.5%),and biomass(67.4%and 74.7%)of poplar cutting seedlings,compared with natural vegetation management.Compared to natural vegetation,soil organic matter(SOM)of barnyardgrass and sesbania covered soil significantly increased by 12.4%and 18.7%at N1,respectively.In addition,soil total N(TN)content was significantly increased by 15.8%in barnyardgrass planted at N0.The soil ammonium N(NH_(4)^(+)-N)content decreased with the planting of barnyardgrass and sesbania across all levels of N application.At N0.5,the nitrate N(NO_(3)^(−)-N)content of soil planted with barnyardgrass significantly increased compared to both the natural vegetation and the sesbania groups.Compared to the natural vegetation,the soil available phosphorus(AP)content of the barnyardgrass group significantly increasing by 78.8%at N0.5,soil available potassium(AK)content was significantly reduced by 12.5%in the sesbania group at N0 and increased by 24.1%in the barnyardgrass group at N1.We found that cumulative NH3 emissions were significantly higher in all treatment groups at the N1 level than that at the N0.5 level,while the differences among the three plants treated were not significant.The results suggest that both barnyardgrass and sesbania promote seedling growth in the short term,while also increase certain properties.Therefore,effective herb management during the seedling stage is recommended in nurseries to support seedling growth and retain soil fertility.
基金Supported by Quality Engineering Teaching Research Project of Anhui Agricultural University(2023aujyxm013,2023aujyxm002)Research Project on Quality Engineering Experimental Teaching and Teaching Laboratory Construction of Anhui Agricultural University(2024ausyyj002)Quality Engineering Teaching Research Project of Anhui Province(2020jyxm0504,2024jyxm0141).
文摘In the context of"high-quality development"construction,the establishment and operational status of the national-level scientific research platforms at provincial universities—specifically,the large-scale instrument sharing platforms—are critically linked to the production of scientific research outcomes and the development of interdisciplinary talents.This study focuses on the public platform of the State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization,aiming to innovate the management system,establish a technical platform,effectively integrate resources,and actively engage graduate students in the platform management.While ensuring the efficient operation of the large-scale instrument platform at state key laboratories,it offers a novel approach to cultivating graduate students into versatile talents.
基金Supported by Innovation Project of Guangxi Graduate Education of Guangxi University of Chinese Medicine(YJSJG201602)Special Project on Innovation and Entrepreneurship Education in Colleges and Universities under the"14 th Five-Year Plan"of Guangxi Education Science(2022ZJY2965).
文摘The cultivation of innovative talents has become the primary objective of talent development in colleges and universities and is also essential for the personal development of master's degree students.Ethnic medicine,a key discipline at Guangxi University of Chinese Medicine,aims to cultivate high-level master's degree talents who possess comprehensive qualities,strong innovative capabilities,and distinctive characteristics of Zhuang medicine.This program is designed to better serve the local economic development and social needs of Guangxi,thereby promoting the substantive advancement of higher education within traditional medical institutions.
文摘THE peony,known as the“king of flowers”in China,is one of the country’s most celebrated flowers.Luoyang,a historically significant city in Henan Province,is widely considered the birthplace of peonies and a center of peony culture,which originated in the city over 1,600 years ago.Today,the city boasts enormous peony varieties,a long cultivation history,and a rich culture surrounding the flower.
基金Supported by Special Fund for National Modern Agricultural Industry Technology System Construction(CARS-04-CES16).
文摘As one of the world's three major food crops and an important economic and oil crop,soybean plays a crucial role in ensuring food safety.In recent years,there are many problems in soybean cultivation,production and processing.In view of this situation,this paper comprehensively expounded and decomposed the cultivation situation,existing problems,specific countermeasures and conclusions,so as to re-recognize them.This study provides reference materials for the sustainable and healthy development of the soybean industry.
基金Global Change Program of National Key Research and Development Program of China,No.2020YFA0607700National Natural Science Foundation of China,No.T2192954,No.42488201,No.42177180。
文摘The Liaohe River Valley was one of the key centers of the origination and development of agriculture in northern China during the Holocene.To understand the long-term interaction among the evolutions of climate,agriculture,and human activities,it is essential to quantitatively reconstruct the spatiotemporal changes in regional prehistoric human land use.In this study,known archaeological sites and a prehistoric land use model(PLUM)were combined to reconstruct changes in land use in the Liaohe River Valley during 8-2 ka BP from a quantitative perspective.The land use area experienced two stages of increase(during 8-5 ka BP and after 4 ka BP)and one stage of decrease(during 5-4 ka BP);these periods were characterized by spatial expansions and contractions.The land use intensity level differed significantly in the western and eastern parts of the valley before 4 ka BP,but the situation changed as the distribution center of the human activity shifted to the southern part of the valley around 5-4 ka BP.Overall,the spatial and temporal changes in the land use areas in both the western and eastern parts of the valley responded well to variations in precipitation during 8-2 ka BP,which potentially provides useful insights into understanding the responses of human activity to future climate change.
文摘In the context of the new era,deepening education reform and improving the quality of talent cultivation are important measures for universities to align with industry trends and the development needs of students.With the continuous growth of the economy,the construction industry is undergoing rapid development and transformation,and there is an increasing demand for high-quality and high-level applied talents,which poses certain challenges to the architecture majors in universities.Therefore,universities should actively follow the industry development trends and the characteristics of talents,clarify the talent cultivation objectives,optimize the professional teaching system,and promote the high-quality development of education.The cultivation of applied talents in the architecture major of universities is not only an internal requirement for the development of the construction industry but also an important part of the country’s innovation-driven development strategy.It is of great significance for promoting scientific and technological progress,enhancing cultural confidence,and promoting the comprehensive development of the economy and society.
文摘Significant variations in global temperatures and weather patterns over time are known as climate change.Although it occurs naturally,human activities—particularly the burning of fossil fuels,deforestation,and industrial processes—are accelerating these changes,which have various detrimental effects on the environment.This review aims to highlight the edapho-climatic requirements of this cactus and the advantages and challenges of its cultivation to mitigate climate change.The prickly pear cactus is a plant with numerous financial and environmental advantages.It needs well-draining,sandy or gravelly soil to avoid root rot and do best in full sun.With a strong tolerance for dryness,they thrive in arid or semi-arid regions with scorching summers and prefer sparing watering.Despite being suited to tropical climates,some species can tolerate freezing temperatures and sporadic frost.Once established,these hardy plants require little care and thrive in nutrient-poor soils,which makes them perfect for xeriscaping or challenging growing environments.Because of its high water use efficiency ratio and low water requirements,prickly pear can be grown in marginally dry and semi-arid areas.The cactus does contribute to the ecological and socioeconomic fight against climate change.For instance,it supports sustainable agriculture,biodiversity preservation,soil restoration,carbon sequestration,and effective water usage.Demarcating dry and semi-arid zones and fostering employment in these areas is beneficial from a socioeconomic standpoint.The prickly pear’s traditional cultural heritage supports its current economic function as a crop that can withstand drought.While ecological threats necessitate balanced management,this adaptability promotes sustainable growth.Innovations in bioenergy and value-added goods build on its historical applications,increasing its socioeconomic advantages and,eventually,its worldwide significance.
文摘Carrots have an important role for a life of the men.Everybody can use carrots in the meals or the drinks,he uses carrots such as the medicaments to cure many diseases,too.Carrots are very necessary,we can tell the following effects:carrots help improve eyesight,especially,carrots contain vitamin A,essential nutrients for health of the eyes.When our bodies have the vitamin A deficiency in the long time,visual cells in the eye retina can been hurt,make dry eyes,cause the visual disturbances and it is serious that everyone does not see.Carrots support to cure the diabetes;help strengthen the immunity;improve the health cardiovascular;help beautiful healthy skin;can reduce the risk of cancer.Besides,everyone drinks carrot juice to stimulate hair growth and stabilize blood pressure.Carrots contain a lot of beta carotene,fiber,vitamin K1,potassium as well as antioxidants and are grown popularly in all regions of Vietnam.The cultivating soil,irrigating water and carrot samples were collected in Loc Thanh village,Bao Lam district,Lam Dong province and some indicators were analyzed.The results showed that the soil sample was poor in nutrient by N,P,K indicators that were lower than the standards;but pH,Al3+and Fe3+were higher than the standards.The water sample did not contain the toxic heavy metals such as:As,Pb,Cd;but the indicators of Cu,Zn,Fe were higher than the standards.For the carrot sample,the indicators of As,Pb,Cu,Zn exceeded the allowable threshold;the contents of lipid and protein were lower than the standards but NO3-was within allowable standards.However,analysis of carrot samples grown by us using organic methods showed that some heavy metal indicators such as:As,Pb,Cu were lower than allowable standards.This showed that it is necessary to choose the type of soil,water and organic method for growing carrots in order to harvest good quality carrots and safety for users.We studied an environment of soil,water and analyzed the carrots to find a nutrition level,nutritional value and the metal accumulation in order to improve a life of men.
基金supported by grants from the Mona Reclamation Experimental Project WAPDA,Pakistan.Researchers Supporting Project Number(RSP2024R410),King Saud University,Riyadh,Saudi Arabia.
文摘Wheat(Triticum aestivum L.)is a staple crop critical for global food security,yet its productivity is significantly affected by abiotic stresses such as drought,salinity,and waterlogging,which are exacerbated by climate change.This study evaluated the effects of these stresses on vegetative growth,physiological responses,and yield.Field experiments were conducted using a Randomized Complete Block Design(RCBD)at the Mona Reclamation Experimental Project(MREP),WAPDA,Bhalwal,Sargodha,Punjab Pakistan.Stress treatments included three levels of drought(25%,50%,and 75%field capacity),salinity(4,8,and 12 dS/m),and waterlogging(24,48,and 72 h).Key parameters measured included plant height,leaf area,tiller number,stomatal conductance,chlorophyll content,and antioxidant enzyme activities.The results revealed that drought stress caused a 46%reduction in yield,while salinity and waterlogging reduced yield by 54%and 35%,respectively,with statistically significant differences(p 0.05).Key<physiological changes included a significant reduction in stomatal conductance(from 0.55 to 0.15 mmol m^(2)/s under drought stress,p 0.01)and chlorophyll content(from 48 to 28 SPAD units under drought,p 0.01).Biochemical<<responses indicated elevated levels of malondialdehyde(MDA)and hydrogen peroxide(H2O2),with significant increases in antioxidant enzyme activities,particularly superoxide dismutase(SOD)and catalase(CAT).These findings underscore the need for developing stress-tolerant wheat varieties and implementing agronomic practices to mitigate the impact of abiotic stresses on wheat yield.
文摘Climate variability significantly impacts agricultural water resources,particularly in regions like Vietnam's Plain of Reeds that heavily utilize rain-fed conditions.This study employs the FAO-AquaCrop model to estimate current and future irrigation water needs for rice cultivation in this critical subregion,aiming to identify optimal sowing schedules(OSS)that enhance rainwater utilization and reduce irrigation dependency.The model was driven by current climate data and future projections(2041-2070 and 2071-2099)derived from downscaled Global Circulation Models under RCP4.5 and RCP8.5 scenarios.The AquaCrop model demonstrated robust performance during validation and calibration,with d-values(0.82-0.93)and R²values(0.85-0.92)indicating strong predictive accuracy for rice yield.Simulation results for efficient irrigation water potential(IWP)under RCP4.5 revealed that strategic shifts in sowing dates can substantially alter water requirements;for instance,advancing the winter-spring sowing to December 5th decreased IWP by 15.6%in the 2041-2070 period,while delaying summer-autumn crop sowing to April 20th increased IWP by 48.6%due to greater reliance on irrigation as rainfall patterns shift.Similar dynamic responses were observed for the 2071-2099 period and for autumn-winter crops.These findings underscore that AquaCrop modeling can effectively predict future irrigation needs and that adjusting cultivation calendars presents a viable,low-cost adaptation strategy.This approach allows farmers in the Plain of Reeds to optimize rainwater use,thereby reducing dependency on supplementary irrigation and mitigating the adverse impacts of climate variability,contributing to more sustainable agricultural water management.
基金supported by the 2023 Sichuan Province Higher Education Talent Cultivation and Teaching Reform Major Project“Exploration and Practice of Interdisciplinary and Integrated Industrial Software Talent Cultivation Model”(JG2023-14)the Sichuan University Higher Education Teaching Reform Project(10th Phase)Research and Exploration of Practical Teaching Mode under the New Major Background of“Cross Disciplinary and Integration”(SCU10128)。
文摘To address the severe challenges posed by the international situation and meet the needs of the national major development strategies,the traditional software engineering talent cultivation model lacks interdisciplinary education focused on specific fields,making it difficult to cultivate engineering leaders with multidisciplinary backgrounds who are capable of solving complex real-world problems.To solve this problem,based on the decade-long interdisciplinary talent cultivation achievements of the College of Software Engineering at Sichuan University,this article proposes the“Software Engineering+”innovative talent cultivation paradigm.It provides an analysis through professional construction of interdisciplinary talents,the design of talent cultivation frameworks,the formulation of cultivation plans,the establishment of interdisciplinary curriculum systems,the reform of teaching modes,and the improvement of institutional systems.Scientific solutions are proposed,and five project models implemented and operated by the College of Software Engineering at Sichuan University are listed as practical examples,offering significant reference value.
基金supported by grants from the National Science Foundation of China to Yanwen Zhang(32272757,31972363)grants from Liaoning Provincial Department of Education Project to Dandan Wang(JYTMS20230698)grants from the Liaoning Provincial Science and Technology.Fund Project to Dandan Wang(2023JH2/101700200).
文摘Taxus cuspidata,a rare species of the Taxus genus,and its wild resources are under severe threat.The development of cultivated species has become an important strategy to replace wild species.The objective of this work was to elucidate the differences in secondary metabolite accumulation,particularly in the paclitaxel biosynthesis pathway,between wild and cultivated species.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)and RNA sequencing(RNA-Seq)technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T.cuspidata.The results showed that the content of paclitaxel in cultivated species was significantly higher than in wild species,reaching 1.67 times that of the latter(p 0.01).Additionally,the<content of key paclitaxel precursors,GGPP and 10-deacetylbaccatin III,in cultivated species was 1.94 times(p 0.05)<and 1.71 times(p 0.01)higher than in wild species,respectively.Transcriptomic analysis identified 2606 differentially<expressed genes(DEGs),among which key enzyme genes related to paclitaxel biosynthesis(such as DXS,DXR,GGPS,etc.)were generally upregulated in cultivated species.Multiple key enzyme genes in both the 2-C-methyl-D-erythritol 4-phosphate pathway(MEP)and paclitaxel biosynthesis pathways were significantly upregulated in cultivated species.Conversely,genes and metabolites related to sugar metabolism were found to be higher in content in wild species.These findings reveal the significant advantage of cultivated species in paclitaxel production capacity,providing new insights into the metabolic regulation mechanisms during yew domestication.This has important implications for optimizing paclitaxel biosynthesis and guiding future improvements in T.cuspidata cultivars.
基金supported by the National Key Research and Development Program(2023YFF1001500)the Local Financial Funds of National Agricultural Science and Technology Center,Chengdu(NASC2022KR02,NASC2023TD08,NASC2021ST08,NASC2021PC04,NASC2022KR07,NASC2022KR06,and NASC2023ST04)+2 种基金the Agricultural Science and Technology Innova-tion Program(ASTIP-34-IUA-01,ASTIP-34-IUA-02,ASTIP-IUA-2023003,and ASTIP2024-34-IUA-09)the Central Public-interest Scientific Institution Basal Research Fund(Y2023YJ07 and SZ202403)the Sichuan Science and Technology Program(2023YFN003,2024NSFC1261,2023YFQ0100,and 2023ZYD0089).
文摘Rice(Oryza sativa L.)plays a pivotal role in global food security,yet its breeding is constrained by its long generation time and seasonality.To enhance rice breeding efficiency and meet future food demands,we have developed a vertical hydroponic breeding system integrated with light-emitting diodes(LEDs)light-ing in a closed plant factory(PF),which significantly accelerates rice growth and generation advance-ment.The results show that indica rice can be harvested as early as after 63 days of cultivation,a 50%reduction compared with field cultivation,enabling the annual harvesting of 5-6 generations within the PF.A hyperspectral imaging(HSI)system and attenuated total reflectance infrared(ATR-IR)spec-troscopy were further employed to characterize the chemical composition of the PF-and field-cultivated rice.Metabolomics analysis with ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and gas chromatography-mass spectrometry(GC-MS)revealed that,com-pared with the field-cultivated rice,the PF-cultivated rice exhibited an up-regulation of total phenolic acids along with 68 non-volatile and 19 volatile metabolites,such as isovitexin,succinic acid,and methylillicinone F.Overall,this study reveals the unique metabolic profile of PF-cultivated rice and high-lights the potential of PFs to accelerate the breeding of crops such as rice,offering an innovative agricul-tural strategy to support food security in the face of global population growth and climate change.
基金Supported by Undergraduate Education and Teaching Reform Research Project of Chengdu University(XJJG-20242025228)Sichuan Genuine Medicinal Materials and Traditional Chinese Medicine Innovation Team(SCCXTD-2025-19)Sichuan Science and Technology Program(2021YFYZ0012).
文摘The rapid advancement of the bioeconomy imposes increasingly stringent demands on bioengineering education.Drawing on data from the 2025 Chinese Undergraduate Employment Report and related sources,this study revealed that while employment placement rates for bioengineering graduates remain stable,starting salaries exhibit limited growth and career trajectories lack clarity.These challenges originate from a curriculum lagging behind technological progress,inadequate practical training,and a mismatch between student competencies and industry expectations.To address these issues,this paper proposed a strategic framework grounded in"demand-driven design,industry-education integration,and competence-centered development".Key strategies include dynamic curriculum renewal,collaborative university-industry training,holistic competency development,and personalized student support,which collectively aim at enhancing graduates employment competitiveness and long-term professional sustainability.
基金supported in part by Provincial Teaching Reform Research Project of Undergraduate Colleges and Universities in Hubei Province(No.2024419)Education Department Science and Technology Planning Project(No.Q20212204)Hubei University of Economics Youth Key Project(No.XJZD202106).
文摘In the context of the new quality productive forces,the advancement of finance and economics institutions relies heavily on the support of engineering disciplines to foster synergies between new engineering and business innovation.Based on the requirements for skilled manpower for the new financial development,we have initiated an initial inquiry into establishing artificial intelligence(AI)major in economic universities under the framework of the new engineering paradigm.From the national policies and the development rules of traditional advantageous majors in universities,we analyze the current challenges and opportunities in the construction of artificial intelligence majors and explore the talent cultivation model of artificial intelligence majors in economic universities under the framework of the integrated development of“New Engineering”and“New Finance”.In conclusion,we propose some viable solutions by giving a case study at Hubei University of Economics.
基金supported by the National Natural Science Foundation of China(Grant No.82260925)Yunnan Provincial High-level Scientific and Technological Talents and Innovative Teams Selection Special Project(Grant No.202305AS350007)+2 种基金Yunnan Provincial Major Scientific and Technological Special Project Program for Biomedicine(Grant No.202402AA310028)Yunnan Province’s High-level Talent Cultivation Project for Successors in Traditional Chinese Medicine Disciplines(Yun Cai She[2024]No.103)Yunnan Provincial Key Laboratory of Dai and Yi Medicine Open Research Topics(2024SS24010).
文摘Chinese medicine,as a treasure of China’s traditional culture,has a long history and deep cultural heritage,and talents are the first resource for the development of Chinese medicine.In ancient times,there were many famous Chinese medicine practitioners with excellent medical skills and fame,and their main ways of success were through teaching and family tradition.The cultivation of high medical ethics,benevolence and love,and helping the world and the people is the source of motivation for success;familiarizing oneself with the classics,understanding the humanities,and being knowledgeable about things past and present is an important way for success;practicing diligently,learning from all the strengths,and observing the right and innovating is the key to success.This paper analyzes the growth path of ancient Chinese medicine masters and discusses the advantages and shortcomings of contemporary Chinese medicine talent training,with a view to providing certain references for the cultivation of Chinese medicine talents.
基金he National Key Research and Development Program of China(2022YFD2300304)the National Natural Science Foundation of China(31671617)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.
