Boolean logic devices play a key role in both traditional and nontraditional molecular logic circuits. This kind of binary logic, in which each bit is coded by （0, 1）, has only two output states--on or off （or high...Boolean logic devices play a key role in both traditional and nontraditional molecular logic circuits. This kind of binary logic, in which each bit is coded by （0, 1）, has only two output states--on or off （or high/low）. Because of the finite computing capacity and variation, it is facing challenges from multivalued logic gates while processing high-density or uncertain/imprecise information. However, a low-cost, simple, and universal system that can perform different multivalued logic computations has not yet been developed, and remains a concept for further study. Herein, taking the ternary OR and INHIBIT logic gates as model devices, we present the fabrication of a novel simple, fast, label-flee, and nanoquencher-free system for multivalued DNA logic gates using poly-thymine （T） templated copper nanoparticles （CuNPs） as signal reporters. The mixture of Cu2~ and ascorbic acid （AA） is taken as a universal platform for all ternary logic gates. Different kinds of poly-T strands and delicately designed complementary poly-adenine （A） strands are alternatively applied as ternary inputs to exhibit the ternary output states （low/0, medium/1, high/2）. Notably, there are no nanoquenchers in this platform as poly-A strands can function as not only inputs but also efficient inhibitors of poly-T templated CuNPs. Moreover, all DNA are unlabeled single-strand DNA that do not need sophisticated labeling procedures or sequence design. The above design greatly reduces the operating time, costs, and complexity. More importantly, the ternary logic computations can be completed within 20 min because of the fast formation of CuNPs, and all of them share the same threshold values.展开更多
Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in bi...Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles(Cu NPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol(MI-h PG) coating via a simple dip-coating method.This hyperbranched polyglycerol with 10 % catechol groups’ modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone(PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated Cu NPs-incorporated PCL membrane [PCL-(MI-h PG)-Cu NPs]was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-h PG)-Cu NPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing Cu NPs.展开更多
Composite structures are sensitive to impact damage in practical engineering.Electric resistance change method(ERCM)is an ideal technique for damage monitoring of composite structures.Due to the anisotropy of fiber-re...Composite structures are sensitive to impact damage in practical engineering.Electric resistance change method(ERCM)is an ideal technique for damage monitoring of composite structures.Due to the anisotropy of fiber-resin matrix composites,impact location monitoring is difficult,and research on impact location of fiber composite laminates(FRPs)is limited.A preparation method of MXene/CNT/CuNps thin film sensor is proposed.According to the modeling simulation and theoretical calculation,the resistance change characteristics of the thin film sensor are obtained,the relationship between the impact distance and the resistance change is established,and the sensor array is designed.A three-point localization algorithm and a weight function compensation localization algorithm are proposed,which can improve the imaging accuracy of the impact position.The impact point location was observed and analyzed using ultrasonic C-scan technology.The results show that the weight function compensation positioning algorithm can accurately locate the impact of the composite structure,and the error in the X direction is 7.1%,the error in the Y direction is 0.03%,which verifies the effectiveness of the method.展开更多
基金This work was supported by the National Natural Science Foundation of China （Nos. 21375123, 21427811 and 21675151）.
文摘Boolean logic devices play a key role in both traditional and nontraditional molecular logic circuits. This kind of binary logic, in which each bit is coded by （0, 1）, has only two output states--on or off （or high/low）. Because of the finite computing capacity and variation, it is facing challenges from multivalued logic gates while processing high-density or uncertain/imprecise information. However, a low-cost, simple, and universal system that can perform different multivalued logic computations has not yet been developed, and remains a concept for further study. Herein, taking the ternary OR and INHIBIT logic gates as model devices, we present the fabrication of a novel simple, fast, label-flee, and nanoquencher-free system for multivalued DNA logic gates using poly-thymine （T） templated copper nanoparticles （CuNPs） as signal reporters. The mixture of Cu2~ and ascorbic acid （AA） is taken as a universal platform for all ternary logic gates. Different kinds of poly-T strands and delicately designed complementary poly-adenine （A） strands are alternatively applied as ternary inputs to exhibit the ternary output states （low/0, medium/1, high/2）. Notably, there are no nanoquenchers in this platform as poly-A strands can function as not only inputs but also efficient inhibitors of poly-T templated CuNPs. Moreover, all DNA are unlabeled single-strand DNA that do not need sophisticated labeling procedures or sequence design. The above design greatly reduces the operating time, costs, and complexity. More importantly, the ternary logic computations can be completed within 20 min because of the fast formation of CuNPs, and all of them share the same threshold values.
基金financially supported by SFB 765 and the Focus Area Nanoscale of Freie Universitat Berlinfinancial support from China Scholarship Councilsupported by the DFG and Dr. Pamela Winchester (Freie Universitat Berlin, Germany) for language polishing。
文摘Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles(Cu NPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol(MI-h PG) coating via a simple dip-coating method.This hyperbranched polyglycerol with 10 % catechol groups’ modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone(PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated Cu NPs-incorporated PCL membrane [PCL-(MI-h PG)-Cu NPs]was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-h PG)-Cu NPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing Cu NPs.
基金This work was financially supported by National Natural Science Foundation of China(11902204)Liaoning Revitalization Talents Program(XLYC2007118)+3 种基金Aeronautical Science Foundation(201903054001)Shenyang Youth Technological Innovation Talent Project(RC200030),Shenyang Natural Science Foundation Project(22-315-6-07)Education Department of Liaoning Province’s Item(LJKQZ 20222263)Basic Scientific Research Project of Liaoning Provincial Department of Education(LJKMZ20220566).
文摘Composite structures are sensitive to impact damage in practical engineering.Electric resistance change method(ERCM)is an ideal technique for damage monitoring of composite structures.Due to the anisotropy of fiber-resin matrix composites,impact location monitoring is difficult,and research on impact location of fiber composite laminates(FRPs)is limited.A preparation method of MXene/CNT/CuNps thin film sensor is proposed.According to the modeling simulation and theoretical calculation,the resistance change characteristics of the thin film sensor are obtained,the relationship between the impact distance and the resistance change is established,and the sensor array is designed.A three-point localization algorithm and a weight function compensation localization algorithm are proposed,which can improve the imaging accuracy of the impact position.The impact point location was observed and analyzed using ultrasonic C-scan technology.The results show that the weight function compensation positioning algorithm can accurately locate the impact of the composite structure,and the error in the X direction is 7.1%,the error in the Y direction is 0.03%,which verifies the effectiveness of the method.
