近红外量子点因其优异的组织穿透性和较低的光损伤,广泛应用于生物成像等领域。本文制备了一种水相CuInS2/ZnS-FA量子点,该量子点在近红外区域表现出优异的光学性能,具有良好的荧光稳定性和水相分散性。生物毒性测试结果显示其具有良好...近红外量子点因其优异的组织穿透性和较低的光损伤,广泛应用于生物成像等领域。本文制备了一种水相CuInS2/ZnS-FA量子点,该量子点在近红外区域表现出优异的光学性能,具有良好的荧光稳定性和水相分散性。生物毒性测试结果显示其具有良好的生物兼容性,表明该材料在生物医学成像等领域具有应用潜力。Near-infrared quantum dots are widely used in fields such as biological imaging due to their excellent tissue penetration and low light-induced damage. In this study, a water-based CuInS2/ZnS-FA quantum dot was synthesized, which exhibits outstanding optical performance in the near-infrared region, with good fluorescence stability and water dispersion. Biotoxicity tests demonstrate that the quantum dots have good biocompatibility, indicating that this material has potential applications in fields such as biomedical imaging.展开更多
采用逐步热注射法合成了用于白光LED的Cu In S2/Zn S(CIS/Zn S)核壳结构量子点。通过调整Cu/In的比率,在Cu In S2(CIS)量子点的基础上,合成了发射波长在570~650 nm之间可调的CIS/Zn S量子点。与CIS量子点的低量子产率相比,具有核壳结构...采用逐步热注射法合成了用于白光LED的Cu In S2/Zn S(CIS/Zn S)核壳结构量子点。通过调整Cu/In的比率,在Cu In S2(CIS)量子点的基础上,合成了发射波长在570~650 nm之间可调的CIS/Zn S量子点。与CIS量子点的低量子产率相比,具有核壳结构的CIS/Zn S量子点的量子产率达到了78%。通过在黄光荧光粉YAG∶Ce3+表面旋涂CIS/Zn S量子点的方式制备了暖白光LED器件。在工作电流为10 m A时,暖白光LED的发光效率达到了244.58 lm/W。由于CIS/Zn S量子点的加入,所制备的白光LED器件的显色指数达到86.7且发光颜色向暖色调发生了转移,相应的色坐标为(0.340 6,0.369 0)。展开更多
BACKGROUND： The oncogenesis of hepatocellular carcinoma（HCC） is not clear. The current methods of the pertinent studies are not precise and sensitive. The present study was to use liver cancer cell line to explore ...BACKGROUND： The oncogenesis of hepatocellular carcinoma（HCC） is not clear. The current methods of the pertinent studies are not precise and sensitive. The present study was to use liver cancer cell line to explore the bio-compatibility and cytotoxicity of ternary quantum dots（QDs） probe and to evaluate the possible application of QDs in HCC.METHODS： CuInS_2-ZnS-AFP fluorescence probe was designed and synthesized to label the liver cancer cell HepG 2. The cytotoxicity of CuInS_2-ZnS-AFP probe was evaluated by MTT experiments and flow cytometry. RESULTS： The labeling experiments indicated that CuInS_2-ZnS QDs conjugated with AFP antibody could enter HepG 2 cells effectively and emit intensive yellow fluorescence by ultraviolet excitation without changing cellular morphology. Toxicity tests suggested that the cytotoxicity of CuInS_2-ZnS-AFP probe was significantly lower than that of CdT e-ZnS-AFP probe（t test, F=0.8, T=-69.326, P〈0.001）. For CuInS_2-ZnS-AFP probe, timeeffect relationship was presented in intermediate concentration（〉20%） groups（P〈0.05） and dose-effect relationship was presented in almost all of the groups（P〈0.05）. CONCLUSION： CuInS_2-ZnS-AFP QDs probe had better biocompatibility and lower cytotoxicity compared with CdT e-ZnS-AFP probe, and could be used for imaging the living cells in vitro.展开更多
文摘近红外量子点因其优异的组织穿透性和较低的光损伤,广泛应用于生物成像等领域。本文制备了一种水相CuInS2/ZnS-FA量子点,该量子点在近红外区域表现出优异的光学性能,具有良好的荧光稳定性和水相分散性。生物毒性测试结果显示其具有良好的生物兼容性,表明该材料在生物医学成像等领域具有应用潜力。Near-infrared quantum dots are widely used in fields such as biological imaging due to their excellent tissue penetration and low light-induced damage. In this study, a water-based CuInS2/ZnS-FA quantum dot was synthesized, which exhibits outstanding optical performance in the near-infrared region, with good fluorescence stability and water dispersion. Biotoxicity tests demonstrate that the quantum dots have good biocompatibility, indicating that this material has potential applications in fields such as biomedical imaging.
基金supported by grants from the Nation al Natural Science Foundation of China(51272246 and 81172082)
文摘BACKGROUND： The oncogenesis of hepatocellular carcinoma（HCC） is not clear. The current methods of the pertinent studies are not precise and sensitive. The present study was to use liver cancer cell line to explore the bio-compatibility and cytotoxicity of ternary quantum dots（QDs） probe and to evaluate the possible application of QDs in HCC.METHODS： CuInS_2-ZnS-AFP fluorescence probe was designed and synthesized to label the liver cancer cell HepG 2. The cytotoxicity of CuInS_2-ZnS-AFP probe was evaluated by MTT experiments and flow cytometry. RESULTS： The labeling experiments indicated that CuInS_2-ZnS QDs conjugated with AFP antibody could enter HepG 2 cells effectively and emit intensive yellow fluorescence by ultraviolet excitation without changing cellular morphology. Toxicity tests suggested that the cytotoxicity of CuInS_2-ZnS-AFP probe was significantly lower than that of CdT e-ZnS-AFP probe（t test, F=0.8, T=-69.326, P〈0.001）. For CuInS_2-ZnS-AFP probe, timeeffect relationship was presented in intermediate concentration（〉20%） groups（P〈0.05） and dose-effect relationship was presented in almost all of the groups（P〈0.05）. CONCLUSION： CuInS_2-ZnS-AFP QDs probe had better biocompatibility and lower cytotoxicity compared with CdT e-ZnS-AFP probe, and could be used for imaging the living cells in vitro.
