[Objectives]To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii.[Methods]The content of caffeic acid in C.buchananii leaves was determined by high performance li...[Objectives]To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii.[Methods]The content of caffeic acid in C.buchananii leaves was determined by high performance liquid chromatography(HPLC).[Results]The results of HPLC determination showed that the RSD values were less than 3%,and the content of caffeic acid in 10 batches of C.buchananii leaves was in the range of 0.0965%-0.4772%.[Conclusions]This method is accurate and reliable,has good linear relationship and high precision,and can be used for quality evaluation of C.buchananii.展开更多
[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.m...[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.展开更多
[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transfor...[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transform infrared spectroscopy. The experimental data were clustered by Omic software and Ward method. [Results] The infrared fingerprints of C. buchananii were obtained, and the similarity was all greater than 0.9. [Conclusions] The established method has good stability and controllability and can be used for the identification of C. buchananii.展开更多
[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of c...[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of chemical liver injury was established by D-GalN, and the mice with D-GalN-induced liver injury were randomly divided into the blank group, model group, positive control group and drug-administered groups. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum of the mice in each group and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver tissue of the mice were determined, and the liver pathological changes were observed. [Results] The extracts of the Zhuang medicinal herb C. buchananii could reduce the serum ALT and AST levels(P<0.05), increase the SOD content in the liver tissue of mice(P<0.05), and decrease the MDA level(P<0.05). [Conclusions] The Zhuang medicinal herb C. buchananii has a good protective effect on D-GalN-induced liver injury in mice.展开更多
基金Supported by Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi(2019KY0324)Graduate Project of Guangxi University of Chinese Medicine(YCSY20190096)+2 种基金Program of Collaborative Innovation Center of Zhuang and Yao Medicine(zyx[2015]-01)Sub-project of Guangxi Key Laboratory of Traditional Chinese Medicine Pharmacology(J16072)Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi University of Chinese Medicine in Guangxi Zhuang Autonomous Region(201910600022).
文摘[Objectives]To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii.[Methods]The content of caffeic acid in C.buchananii leaves was determined by high performance liquid chromatography(HPLC).[Results]The results of HPLC determination showed that the RSD values were less than 3%,and the content of caffeic acid in 10 batches of C.buchananii leaves was in the range of 0.0965%-0.4772%.[Conclusions]This method is accurate and reliable,has good linear relationship and high precision,and can be used for quality evaluation of C.buchananii.
基金Supported by Fundamental Scientific Research Ability Enhancement Project for Young and Middle-Aged Teachers in Universities and Colleges of Guangxi(2019KY0324)Graduate Project of Guangxi University of Chinese Medicine(xjyb019)+2 种基金Project of Zhuang Medicine Collaborative Innovation Center(zyx[2015]-01)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)College Students’Innovative Entrepreneurial Training Program of Guangxi University of Chinese Medicine(201910600022).
文摘[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.
基金Supported by The Basic Ability Improving Project of Young and Middle-aged Teachers in Guangxi(2019KY0324)Graduate Program of Guangxi University of Chinese Medicine(xjyb019)+2 种基金Collaborative Innovation Center for Zhuang and Yao Medicines(zyx[2015]-01)General Project of Guangxi University of Chinese Medicine(P2010016)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)
文摘[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transform infrared spectroscopy. The experimental data were clustered by Omic software and Ward method. [Results] The infrared fingerprints of C. buchananii were obtained, and the similarity was all greater than 0.9. [Conclusions] The established method has good stability and controllability and can be used for the identification of C. buchananii.
基金Supported by The Basic Ability Improving Project of Young and Middle-aged Teachers in Guangxi(2019KY0324)Graduate Program of Guangxi University of Chinese Medicine(YCSY20190096)+2 种基金Collaborative Innovation Center for Zhuang and Yao Medicines(zyx[2015]-01)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)College Students’Innovation and Enterpreneurship Training Program of Guangxi University of Chinese Medicine(201910600022)
文摘[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of chemical liver injury was established by D-GalN, and the mice with D-GalN-induced liver injury were randomly divided into the blank group, model group, positive control group and drug-administered groups. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum of the mice in each group and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver tissue of the mice were determined, and the liver pathological changes were observed. [Results] The extracts of the Zhuang medicinal herb C. buchananii could reduce the serum ALT and AST levels(P<0.05), increase the SOD content in the liver tissue of mice(P<0.05), and decrease the MDA level(P<0.05). [Conclusions] The Zhuang medicinal herb C. buchananii has a good protective effect on D-GalN-induced liver injury in mice.
