The crowding agent induced phase transition of amphiphilic block copolymers in solution was explicitly considered. The influence of the size and the volume fraction of the crowding agent on the phase separation of amp...The crowding agent induced phase transition of amphiphilic block copolymers in solution was explicitly considered. The influence of the size and the volume fraction of the crowding agent on the phase separation of ampbiphilic diblock copolymers is investigated by using self-consistent field theory （SCFT） method. The concentration of the disorder to order transition of the block copolymer decreases when the size of the crowding agent is larger than that of the solvent. The higher volume fraction of the crowding agent will induce the transition of the block copolymer from disorder to order state at a lower concentration. The relation between the size and the volume fraction of the crowding agent is elucidated. When the size of the crowding agent is larger, its volume fraction of the disorder to order transition of the block copolymer will be lower. The conformation of the crowding agent considered as a polymer chain is also studied and compared.展开更多
Colorectal cancer(CRC)is one of the most prevalent forms of cancer.CircRNAs have emerged as promising biomarkers for cancer diagnosis and prognosis evaluation.However,novel circRNAs as potential biomarkers for CRC sti...Colorectal cancer(CRC)is one of the most prevalent forms of cancer.CircRNAs have emerged as promising biomarkers for cancer diagnosis and prognosis evaluation.However,novel circRNAs as potential biomarkers for CRC still need further exploration and validation,and precise detection methods are yet to be developed.Herein,we report for the first time the use of droplets Cas13a to detect the circWDR37 as a biomarker of CRC.The arraystar circRNA microarray assays,functional experiments in vitro and in vivo,and qPCR were performed to discover and validate that circWDR37 is a biomarker for early screening and prognosis evaluation of CRC.A new technology namedμDCR,which accurately detects circWDR37,has been developed by combining microfluidic droplets with CRISPR/Cas13a and recombinase polymerase amplification(RPA).Meanwhile,the role of crowding agent in improving the performance of Cas13a was uncovered.The 4%polyethylene glycol 8000 and 3%dextran-10 significantly improved the response speed and sensitivity of one-pot Cas13a-RPA reaction.The detection limit of circWDR37 byμDCR was found to be 10 copies/mL,which is higher than that of qPCR.The clinical sample findings demonstrated that circWDR37 detection can be utilized to effectively screen for CRC at an early stage and enable accurate assessment of prognosis.CircWDR37 is confirmed as a groundbreaking biomarker for both diagnosis and prognosis evaluation in CRC patients.Furthermore,our innovativeμDCR method for detecting circWDR37 demonstrates impressive attributes such as streamlined operation,rapidity,and high-throughput,making it an optimal technology platform for the noninvasive screening of CRC.展开更多
基金financially supported by the National Natural Science Foundations of China(Nos.20874046,21074053 and 51133002)National Basic Research Program of China(Nos.2010CB923303,2012CB821503)Fundamental Research Funds for the Central Universities(No.1095020515)
文摘The crowding agent induced phase transition of amphiphilic block copolymers in solution was explicitly considered. The influence of the size and the volume fraction of the crowding agent on the phase separation of ampbiphilic diblock copolymers is investigated by using self-consistent field theory （SCFT） method. The concentration of the disorder to order transition of the block copolymer decreases when the size of the crowding agent is larger than that of the solvent. The higher volume fraction of the crowding agent will induce the transition of the block copolymer from disorder to order state at a lower concentration. The relation between the size and the volume fraction of the crowding agent is elucidated. When the size of the crowding agent is larger, its volume fraction of the disorder to order transition of the block copolymer will be lower. The conformation of the crowding agent considered as a polymer chain is also studied and compared.
基金supported by National Key Research and Development Program(Nos.2022YFC2603800 and 2023YFC2306200 to M.L.)National Natural Science Foundation of China(82472374)+2 种基金the Cross-Research Fund of Biomedical Engineering of Shanghai Jiaotong University(YG2024LC02)Foundation of Shanghai Renji Hospital of School of Medicine of Jiaotong University(RJPY-10-005)Public Health System Construction Three-year Action Plan(GWVI-11.1-11).
文摘Colorectal cancer(CRC)is one of the most prevalent forms of cancer.CircRNAs have emerged as promising biomarkers for cancer diagnosis and prognosis evaluation.However,novel circRNAs as potential biomarkers for CRC still need further exploration and validation,and precise detection methods are yet to be developed.Herein,we report for the first time the use of droplets Cas13a to detect the circWDR37 as a biomarker of CRC.The arraystar circRNA microarray assays,functional experiments in vitro and in vivo,and qPCR were performed to discover and validate that circWDR37 is a biomarker for early screening and prognosis evaluation of CRC.A new technology namedμDCR,which accurately detects circWDR37,has been developed by combining microfluidic droplets with CRISPR/Cas13a and recombinase polymerase amplification(RPA).Meanwhile,the role of crowding agent in improving the performance of Cas13a was uncovered.The 4%polyethylene glycol 8000 and 3%dextran-10 significantly improved the response speed and sensitivity of one-pot Cas13a-RPA reaction.The detection limit of circWDR37 byμDCR was found to be 10 copies/mL,which is higher than that of qPCR.The clinical sample findings demonstrated that circWDR37 detection can be utilized to effectively screen for CRC at an early stage and enable accurate assessment of prognosis.CircWDR37 is confirmed as a groundbreaking biomarker for both diagnosis and prognosis evaluation in CRC patients.Furthermore,our innovativeμDCR method for detecting circWDR37 demonstrates impressive attributes such as streamlined operation,rapidity,and high-throughput,making it an optimal technology platform for the noninvasive screening of CRC.
