Objective:To investigate the level of heavy metals(Cu,Zn,Fe Mn Ni,Pb and Cd) in muscles,gills and liver tissue of Croaker fish from oil spilled rivers of Bonny and Finima,Niger Delta region,Nigeria.Methods:Twenty four...Objective:To investigate the level of heavy metals(Cu,Zn,Fe Mn Ni,Pb and Cd) in muscles,gills and liver tissue of Croaker fish from oil spilled rivers of Bonny and Finima,Niger Delta region,Nigeria.Methods:Twenty four Croacker fishes(Genyonemus lineatus) weighing between 250 and 260 g and 24–26 cm length were collected from each sampling sites(Bonny and finima rivers).The frozen fishes were thawed and dried at 105C until they reach a constant weight.The dried samples were homogenized and digested with 10 ml tri-acid mixture(HNO3:HCl O4:H2SO4),and then the digested samples were diluted to 100 ml with deionized water.Heavy metals(Cu,Ni,Zn,Pb,Mn,Iron Fe,and Cd) concentrations were determined by atomic absorption spectrophotometer.Results:Our results revealed that concentrations and distribution patterns of all heavy metals studied varied significantly(P < 0.05) amongst the fish tissues analyzed and sampling sites.Moreover,liver tissue of fish caught from Finima creek accumulated the highest concentrations of Cu(52.64 ± 3.01 mg/g dry wt),Zn(166.50 ± 6.45 mg/g dry wt)and Fe(801.50 ± 14.15 mg/g dry wt) in comparison to the liver of fish caught from Bonny river in which the levels of Cu,Zn and Fe were(45.00 ± 2.79),(49.90 ± 2.91) and(216 ± 6.11 mg/g dry wt),respectively.In addition,Mn,Ni,Pb and Cd exhibited their highest concentrations in gills from both locations.As expected,muscle tissue contained the least concentrations of all metals investigated from both sampling sites.Furthermore,all the metals investigated exhibited highest concentration in fish collected from Finima creek.These abnormal high level heavy metals accumulation observed in this location could be linked to the frequent crude oil spills as well as industrial activity around the area which might get discharged into Finima creek.In general,the mean concentrations of some toxic heavy metals investigated exceed the recommended maximum permissible limits set by the Joint FAO/WHO committee.However,some metals are within the acceptable limits.Conclusions:In conclusion,our data showed the abnormal higher concentration of these metals and this might be toxic to the fish and other aquatic organisms directly or by extension to humans that frequently consumed such contaminated fishes.展开更多
To ascertain the nutrient components and nutritive quality of the flesh of big yellow croaker in three culture conditions （ traditional cage, offshore cage and cage-free）, basic nutritional components,amino acid,fat...To ascertain the nutrient components and nutritive quality of the flesh of big yellow croaker in three culture conditions （ traditional cage, offshore cage and cage-free）, basic nutritional components,amino acid,fatty acid and mineral elements were determined. The results indicated that crude protein in flesh of the big yellow croaker in cage-free culture was higher than that in offshore cage and much higher than that in traditional cage （ P 〈0.05）. Crude fat of the croaker cul- tured in the traditional cage was twice as high as that in cage-free culture, while that in the offshore cage was in the middle. Proline content in the cage-free culture was much higher than that in the offshore cage, and also than that in the traditional cage （P 〈 0.05 ）. There was no significant difference in the content of alanine, methionine and tryptophan （ P 〈 0.05）. Contents of other amino acids had no significant difference between the cage-free culture and offshore cage, but were much lower in the traditional cage （P 〈 0.05 ）. Top six fatty acids were 9-Hexadecenoic acid, palmitic acid,9-Octadecenoic acid, Octadecauoic acid, DHA and EPA. The palmitic acid content was the highest in cage-free culture and in traditional cage, 9-Octadeeenoic acid content was the highest in offshore cage. Content of unsaturat- ed fatty acids in cage-free culture, offshore cage and traditional cage was 63.60,66.32,57.67, respectively, and polyunsaturated fatty acid was 29.10, 28.57, and 24.40. Content of DHA in cage-free culture was significantly higher than that in the offshore and traditional cage. Content of zinc had no significant difference in three culture models. Content of phosphorus had no significant difference between that in cage-free culture and offshore cage, was lower in the traditional cage. The cage-free cultured croakers had the highest content of calcium and phosphorus. Content of selenium was about the same between the offshore cage and the traditional cage stocking, higher in the cage-free culture. This research has considerable application value for identifying quality and sources of the big yellow croakers.展开更多
传统的大黄鱼鱼苗异常行为检测与计数耗时长、效率低,无法满足自动化水产养殖的需求。基于机器视觉技术设计大黄鱼鱼苗异常行为检测计数系统。通过在原始YOLO(You Look Only Once)v5中添加ContextGuided模块和DeepSORT目标追踪算法,对...传统的大黄鱼鱼苗异常行为检测与计数耗时长、效率低,无法满足自动化水产养殖的需求。基于机器视觉技术设计大黄鱼鱼苗异常行为检测计数系统。通过在原始YOLO(You Look Only Once)v5中添加ContextGuided模块和DeepSORT目标追踪算法,对异常行为大黄鱼鱼苗进行精准检测。同时集成实时计数模块,对异常行为大黄鱼鱼苗数量进行统计监测。研究结果表明,基于机器视觉的大黄鱼鱼苗异常行为检测计数系统精确度达到96.4%,召回率达到97.8%,F1分数达到97.1%,平均精度达到98.3%,帧率达到93.46,为水产养殖业提供了一种高效、智能化的鱼苗异常行为监测方案。展开更多
文摘Objective:To investigate the level of heavy metals(Cu,Zn,Fe Mn Ni,Pb and Cd) in muscles,gills and liver tissue of Croaker fish from oil spilled rivers of Bonny and Finima,Niger Delta region,Nigeria.Methods:Twenty four Croacker fishes(Genyonemus lineatus) weighing between 250 and 260 g and 24–26 cm length were collected from each sampling sites(Bonny and finima rivers).The frozen fishes were thawed and dried at 105C until they reach a constant weight.The dried samples were homogenized and digested with 10 ml tri-acid mixture(HNO3:HCl O4:H2SO4),and then the digested samples were diluted to 100 ml with deionized water.Heavy metals(Cu,Ni,Zn,Pb,Mn,Iron Fe,and Cd) concentrations were determined by atomic absorption spectrophotometer.Results:Our results revealed that concentrations and distribution patterns of all heavy metals studied varied significantly(P < 0.05) amongst the fish tissues analyzed and sampling sites.Moreover,liver tissue of fish caught from Finima creek accumulated the highest concentrations of Cu(52.64 ± 3.01 mg/g dry wt),Zn(166.50 ± 6.45 mg/g dry wt)and Fe(801.50 ± 14.15 mg/g dry wt) in comparison to the liver of fish caught from Bonny river in which the levels of Cu,Zn and Fe were(45.00 ± 2.79),(49.90 ± 2.91) and(216 ± 6.11 mg/g dry wt),respectively.In addition,Mn,Ni,Pb and Cd exhibited their highest concentrations in gills from both locations.As expected,muscle tissue contained the least concentrations of all metals investigated from both sampling sites.Furthermore,all the metals investigated exhibited highest concentration in fish collected from Finima creek.These abnormal high level heavy metals accumulation observed in this location could be linked to the frequent crude oil spills as well as industrial activity around the area which might get discharged into Finima creek.In general,the mean concentrations of some toxic heavy metals investigated exceed the recommended maximum permissible limits set by the Joint FAO/WHO committee.However,some metals are within the acceptable limits.Conclusions:In conclusion,our data showed the abnormal higher concentration of these metals and this might be toxic to the fish and other aquatic organisms directly or by extension to humans that frequently consumed such contaminated fishes.
基金Sponsored by Research Program of Zhejiang Provincial Department of Education(Y201328411)Program of Zhejiang Provincial Department of Science and Technology(2009C22007)
文摘To ascertain the nutrient components and nutritive quality of the flesh of big yellow croaker in three culture conditions （ traditional cage, offshore cage and cage-free）, basic nutritional components,amino acid,fatty acid and mineral elements were determined. The results indicated that crude protein in flesh of the big yellow croaker in cage-free culture was higher than that in offshore cage and much higher than that in traditional cage （ P 〈0.05）. Crude fat of the croaker cul- tured in the traditional cage was twice as high as that in cage-free culture, while that in the offshore cage was in the middle. Proline content in the cage-free culture was much higher than that in the offshore cage, and also than that in the traditional cage （P 〈 0.05 ）. There was no significant difference in the content of alanine, methionine and tryptophan （ P 〈 0.05）. Contents of other amino acids had no significant difference between the cage-free culture and offshore cage, but were much lower in the traditional cage （P 〈 0.05 ）. Top six fatty acids were 9-Hexadecenoic acid, palmitic acid,9-Octadecenoic acid, Octadecauoic acid, DHA and EPA. The palmitic acid content was the highest in cage-free culture and in traditional cage, 9-Octadeeenoic acid content was the highest in offshore cage. Content of unsaturat- ed fatty acids in cage-free culture, offshore cage and traditional cage was 63.60,66.32,57.67, respectively, and polyunsaturated fatty acid was 29.10, 28.57, and 24.40. Content of DHA in cage-free culture was significantly higher than that in the offshore and traditional cage. Content of zinc had no significant difference in three culture models. Content of phosphorus had no significant difference between that in cage-free culture and offshore cage, was lower in the traditional cage. The cage-free cultured croakers had the highest content of calcium and phosphorus. Content of selenium was about the same between the offshore cage and the traditional cage stocking, higher in the cage-free culture. This research has considerable application value for identifying quality and sources of the big yellow croakers.
文摘传统的大黄鱼鱼苗异常行为检测与计数耗时长、效率低,无法满足自动化水产养殖的需求。基于机器视觉技术设计大黄鱼鱼苗异常行为检测计数系统。通过在原始YOLO(You Look Only Once)v5中添加ContextGuided模块和DeepSORT目标追踪算法,对异常行为大黄鱼鱼苗进行精准检测。同时集成实时计数模块,对异常行为大黄鱼鱼苗数量进行统计监测。研究结果表明,基于机器视觉的大黄鱼鱼苗异常行为检测计数系统精确度达到96.4%,召回率达到97.8%,F1分数达到97.1%,平均精度达到98.3%,帧率达到93.46,为水产养殖业提供了一种高效、智能化的鱼苗异常行为监测方案。
