Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al....Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).展开更多
Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due...Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due to signal interference from blood autofluorescence.Here,we proposed a universal and accessible bioluminescent CRISPR/Cas(bioLUCas)platform for direct detection of disease biomarkers in whole blood.By employing a specially designed cpHNLucMB reporter,the bioLUCas system converts CRISPR/Cas12a trans-cleavage activity into a ratiometric bioluminescent signal,producing a distinct emission color change.Compared to conventional CRISPR/Cas12a-based sensors,this platform eliminates the need for external light excitation,effectively bypassing blood autofluorescence and offering high sensitivity.Additionally,the visual signal of bioLUCas system allows user-friendly readout methods,such as smartphone.The platform successfully facilitated point-of-care test(POCT)for myeloperoxidase(MPO)in clinical acute myelogenous leukemia(AML)blood samples and hepatitis C virus(HCV)RNA in synthetic blood samples.This work may advance CRISPR/Cas technology for accessible whole-blood disease diagnostics.展开更多
基金supported by the Natural Science Foundation of China(Grant Nos.32172544,32441071)the special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(Grant No.R2023PY-JG003)+4 种基金the earmarked fund for CARS(Grant No.CARS-31-01)Guangdong Special Support Program(Grant No.NYLJ2024010)Guangdong S&T Program(Grant No.2025B0202070005)IAEA CRP D23033the Project from Guangzhou Municipal Science and Technology Bureau(2023B03J0991).
文摘Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).
基金supported by the National Natural Science Foundation of China(22474032,22104032)to Mengyi Xiong and(22234003,21890744)to Xiao-Bing Zhangthe Natural Science Foundation of Shanxi Province(202303021221139)to Xuhua Zhao+1 种基金the China Postdoctoral Science Foundation(2024M761887)to Xuhua Zhaothe Innovation and Entrepreneurship Training Program for College Students(20161469)。
文摘Clustered regularly interspaced short palindromic repeats(CRISPR)systems have achieved significant advancements in precise molecular diagnosis.However,their applications in whole blood detection remain challenging due to signal interference from blood autofluorescence.Here,we proposed a universal and accessible bioluminescent CRISPR/Cas(bioLUCas)platform for direct detection of disease biomarkers in whole blood.By employing a specially designed cpHNLucMB reporter,the bioLUCas system converts CRISPR/Cas12a trans-cleavage activity into a ratiometric bioluminescent signal,producing a distinct emission color change.Compared to conventional CRISPR/Cas12a-based sensors,this platform eliminates the need for external light excitation,effectively bypassing blood autofluorescence and offering high sensitivity.Additionally,the visual signal of bioLUCas system allows user-friendly readout methods,such as smartphone.The platform successfully facilitated point-of-care test(POCT)for myeloperoxidase(MPO)in clinical acute myelogenous leukemia(AML)blood samples and hepatitis C virus(HCV)RNA in synthetic blood samples.This work may advance CRISPR/Cas technology for accessible whole-blood disease diagnostics.
