以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧...以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧稻苗过氧化氢酶 (CAT)活性和抗坏血酸过氧化物酶 (APX)活性下降 ;对谷胱甘肽还原酶 (GR)活性则无明显影响 ;而超氧化物岐化酶 (SOD)和过氧化物酶 (POD)活性前期无明显变化 ,仅在胁迫后期明显下降。盐胁迫下 ,L a Cl3和 CPZ预处理加剧 SOD、 CAT和 APX活性下降与加剧稻苗 MDA含量积累呈显著或极显著正相关。这暗示 Ca2 + · Ca展开更多
Backgrounds:As cancer progresses through various stages of malignancy,metastasis,and drug resistance,the Wnt/-catenin signaling is frequently dysregulated.Despite advancements in medical technology and therapeutic str...Backgrounds:As cancer progresses through various stages of malignancy,metastasis,and drug resistance,the Wnt/-catenin signaling is frequently dysregulated.Despite advancements in medical technology and therapeutic strategies,the prognosis for numerous gastric cancer patients remains unfavorable.Methods:For the analysis of prognostic signature genes associated with Wnt signaling in GC,we used LASSO(least absolute shrinkage and selection operator)regression.To explore the function,cell specificity,and transcriptional regulation of the signature gene Carboxypeptidase Z(CPZ),we conducted co-expression analysis,single-cell RNA sequencing data analysis,transcription factor prediction,and dual luciferase reporter assay.The knockdown and overexpression experiments were also performed to observe the changes in the downstream gene expression,as well as the influence on the biological functions of GC cells.Results:We identified a five-gene signature,including CPZ,Collagen Triple Helix Repeat Containing-1(CTHRC1),Dickkopf-1(DKK1),Epidermal Growth Factor(EGF),and Glypican Proteoglycan-3(GPC3),with risk scores predictive of the prognosis of GC patients.We found that the adipocyte enhancer binding protein 1(AEBP1)and transcription factor 3(TCF3)could interact in the nucleus and synergistically enhance the expression of Wnt signaling-associated genes,including WNT2/FZD2(Wnt family member 2/frizzled class receptor 2)and VIM(vimentin),thus promoting the invasion,migration,and malignant metastasis of GC.Conclusions:Our study offers a precise gene-signature prediction method for the prognosis of GC.We discovered the synergistic effect of AEBP1 and TCF3 in the nucleus on GC metastasis.GC may benefit from the identification of this potential therapeutic target.展开更多
文摘以质膜 Ca2 +通道阻断剂 L a Cl3和钙调素 (Ca M)拮抗剂氯丙嗪 (CPZ)对水稻幼苗预处理以阻碍 Ca2 +· Ca M信使传导后 ,研究了盐胁迫下 ,稻苗保护酶活性的变化。结果表明 :0 .15 mol/ L Na Cl胁迫下 ,L a Cl3和 CPZ预处理显著加剧稻苗过氧化氢酶 (CAT)活性和抗坏血酸过氧化物酶 (APX)活性下降 ;对谷胱甘肽还原酶 (GR)活性则无明显影响 ;而超氧化物岐化酶 (SOD)和过氧化物酶 (POD)活性前期无明显变化 ,仅在胁迫后期明显下降。盐胁迫下 ,L a Cl3和 CPZ预处理加剧 SOD、 CAT和 APX活性下降与加剧稻苗 MDA含量积累呈显著或极显著正相关。这暗示 Ca2 + · Ca
基金funded by the Natural Science Research Project of Anhui Educational Committee(2023AH050650)the Research Fund Program of Anhui Medical University(2023xkj176).
文摘Backgrounds:As cancer progresses through various stages of malignancy,metastasis,and drug resistance,the Wnt/-catenin signaling is frequently dysregulated.Despite advancements in medical technology and therapeutic strategies,the prognosis for numerous gastric cancer patients remains unfavorable.Methods:For the analysis of prognostic signature genes associated with Wnt signaling in GC,we used LASSO(least absolute shrinkage and selection operator)regression.To explore the function,cell specificity,and transcriptional regulation of the signature gene Carboxypeptidase Z(CPZ),we conducted co-expression analysis,single-cell RNA sequencing data analysis,transcription factor prediction,and dual luciferase reporter assay.The knockdown and overexpression experiments were also performed to observe the changes in the downstream gene expression,as well as the influence on the biological functions of GC cells.Results:We identified a five-gene signature,including CPZ,Collagen Triple Helix Repeat Containing-1(CTHRC1),Dickkopf-1(DKK1),Epidermal Growth Factor(EGF),and Glypican Proteoglycan-3(GPC3),with risk scores predictive of the prognosis of GC patients.We found that the adipocyte enhancer binding protein 1(AEBP1)and transcription factor 3(TCF3)could interact in the nucleus and synergistically enhance the expression of Wnt signaling-associated genes,including WNT2/FZD2(Wnt family member 2/frizzled class receptor 2)and VIM(vimentin),thus promoting the invasion,migration,and malignant metastasis of GC.Conclusions:Our study offers a precise gene-signature prediction method for the prognosis of GC.We discovered the synergistic effect of AEBP1 and TCF3 in the nucleus on GC metastasis.GC may benefit from the identification of this potential therapeutic target.
