Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integ...Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.展开更多
目的探讨基于Cox健康行为互动模式(interaction model of client health behavior,IMCHB)的心理护理联合疼痛护理小组模式应用于带状疱疹患者中的效果。方法方便选取2021年9月—2023年9月山东省泰安市中心医院收治的82例带状疱疹患者为...目的探讨基于Cox健康行为互动模式(interaction model of client health behavior,IMCHB)的心理护理联合疼痛护理小组模式应用于带状疱疹患者中的效果。方法方便选取2021年9月—2023年9月山东省泰安市中心医院收治的82例带状疱疹患者为研究对象,依据不同护理方法分为两组,各41例。对照组采取常规护理,观察组实施基于IMCHB的心理护理联合疼痛护理小组模式护理,两组均连续护理1周。比较两组患者心理状态、疼痛程度、舒适度以及生活质量。结果护理后,观察组患者健康问卷、广泛性焦虑障碍量表评分分别为(12.33±1.84)分、(9.19±1.44)分,均低于对照组的(15.51±1.96)分、(12.76±1.52)分,差异均有统计学意义(t=7.574,10.918;P均<0.05)。护理后,观察组两项疼痛评分及生活质量评分低于对照组,舒适度评分高于对照组,差异均有统计学意义(P均<0.05)。结论基于IMCHB的心理护理联合疼痛护理小组模式可缓解带状疱疹患者负面情绪,改善其疼痛程度和舒适度,促进患者生活质量提升。展开更多
基金supported by grants from the Guangxi Natural Science Foundation(2024GXNSFAA010150)the Guangdong Basic and Applied Basic Research Foundation(2022A1515111167).
文摘Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.
文摘目的探讨基于Cox健康行为互动模式(interaction model of client health behavior,IMCHB)的心理护理联合疼痛护理小组模式应用于带状疱疹患者中的效果。方法方便选取2021年9月—2023年9月山东省泰安市中心医院收治的82例带状疱疹患者为研究对象,依据不同护理方法分为两组,各41例。对照组采取常规护理,观察组实施基于IMCHB的心理护理联合疼痛护理小组模式护理,两组均连续护理1周。比较两组患者心理状态、疼痛程度、舒适度以及生活质量。结果护理后,观察组患者健康问卷、广泛性焦虑障碍量表评分分别为(12.33±1.84)分、(9.19±1.44)分,均低于对照组的(15.51±1.96)分、(12.76±1.52)分,差异均有统计学意义(t=7.574,10.918;P均<0.05)。护理后,观察组两项疼痛评分及生活质量评分低于对照组,舒适度评分高于对照组,差异均有统计学意义(P均<0.05)。结论基于IMCHB的心理护理联合疼痛护理小组模式可缓解带状疱疹患者负面情绪,改善其疼痛程度和舒适度,促进患者生活质量提升。
