In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA...In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of C.coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy C.coggygria in Xunlupo than that in diseased ones in 2018.Verticillium dahliae,which is the causal agent of C.coggygria wilt,was detected in five soil samples.In 2018,the bacterial diversity in the rhizosphere soil of diseased C.coggygria in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy C.coggygria under Platycladus orientalis in Langfengting.展开更多
Objective:To assess the analgesic and anti-inflammatory effects of standardized extract of Cotinus coggygria(C.coggygria)in different animal models.Methods:C.coggygria extracts(25,50,and 100 mg/kg)were administered to...Objective:To assess the analgesic and anti-inflammatory effects of standardized extract of Cotinus coggygria(C.coggygria)in different animal models.Methods:C.coggygria extracts(25,50,and 100 mg/kg)were administered to rats and mice(n=6)during hot plate,tail-flick,acetic acid-induced writhing,and formalin tests to determine its analgesic efficacy.The anti-inflammatory activity of C.coggygria extracts was evaluated by histamine and carrageenan-induced paw edema,cotton pellet-induced granuloma,and acetic acid-induced peritoneal capillary dye leakage tests.Results:C.coggygria extracts(50 and 100 mg/kg)significantly alleviated thermal and chemical-induced pain in rodents(P<0.05).It also demonstrated notable anti-inflammatory properties by mitigating histamine and carrageenan-induced paw edema,granuloma deposits,and vascular permeability(P<0.05).Moreover,C.coggygria extracts remarkably reduced TNF-α,IL-1β,IL-6,COX-2,and oxidative stress in rat paws(P<0.05).Carrageenan-induced histological aberrations in hind paw tissues were effectively(P<0.05)mitigated by treatment with C.coggygria extracts.Conclusions:C.coggygria Scop.extracts show analgesic and anti-inflammatory effects via inhibition of COX-2 and inflammatory and oxidative mediators.展开更多
We analyzed the inhibition rates of eight kinds of fungicides to the pathogen of verticillium wilt of smoke tree （Cotinus coggygria） by measuring mycelium growth. Results show that four fungicides （Weijunjing, thio...We analyzed the inhibition rates of eight kinds of fungicides to the pathogen of verticillium wilt of smoke tree （Cotinus coggygria） by measuring mycelium growth. Results show that four fungicides （Weijunjing, thiophanate-methyl 70% WP （wettable powder）, carbendazim 50% WP and Junxianwei） have the best antifungal effects. Three fungicides, Weijunjing, carbendazim 50% WP and Junxianwei, were selected to determine the control of verticillium wilt in potted smoke tree seedlings. We found that the relative efficacy of Weijunjing was up to 74%. Based on the results, Weijunjing and carbendazim 50% WP were prepared for field trials. The results indicate that the relative efficacy of Weijunjing （300 times diluted） and carbendazim 50% WP （400 times diluted） were 66.2% and 48.9% in plot 1 and 26.4% and 31.8% in plot 2 after soil disinfection, and were 224% and 61.8% in plot 3 without soil disinfection in 2007. The relative efficacy of Weijunjing and carbendazim 50% WP were 49.6% and 45.4% in plot 2 in 2008.展开更多
Objective:To investigate the therapeutic effects of aqueous infusion of Cotinus coggygria Scop.leaves(CCLAI)on the dextran sulphate sodium(DSS)-induced ulcerative colitis(UC)in mice.Methods:A total of 56 C57BL16J mice...Objective:To investigate the therapeutic effects of aqueous infusion of Cotinus coggygria Scop.leaves(CCLAI)on the dextran sulphate sodium(DSS)-induced ulcerative colitis(UC)in mice.Methods:A total of 56 C57BL16J mice were divided into 7 groups,including control,DSS model,CCLAI4,CCLAI6,DSS+CCLAI4,DSS+CCLAI6 and DSS+mesalamine groups(n=8).Induction of UC was conducted by 7-day treatment of 3%DSS in mice.CCLAI in 2 different doses(4%and 6%)or mesalamine(250 mg/kg)was administered to C57BL/6J mice orally for 7 consecutive days.The mice in the control group received only tap water.Clinical parameters,antioxidant and anti-inflammatory activities,and histopathological changes in the colon mucosa were examined.Cell proliferatipn index in colon section was determined by immunonistochemical analysis.Colonic cyclooxygenase(COX)-2 and inducible nitric oxide synthase(i NOS)expression levels were detected by Western blot.Results:CCLAI treatment inhibited DSS-induced colon shortening and significantly improved the Disease Activity Index score(P<0.05 or P<0.01).We also found a significant decrease in myeloperoxidase levels,oxidative stress parameters,and neutrophil infiltration index in the experimental groups receiving CCLAI compared to the control group(all P<0.01).In addition,it was observed that CCLAI administration for 7 days decreased the pro-inflammatory cytokines levels including interleukin(IL)-1β,IL-6,interferon-γ,and tumor necrosis factor-αin the colon tissues of mice receiving DSS(P<0.05).Further,CCLAI treatment reduced the COX-2 and i NOS levels with an accompanying decrease in prostaglandin E2 and nitric oxide levels,respectively.Additionally,CCLAI treatment markedly decreased the histological lesion score,which was consistent with the reduction of the pro-inflammatory cytokine levels.Conclusion:CCLAI treatment significantly improved the DSS-induced UC in mice by diminishing histopathological damage in the colonic mucosa and markedly decreased the inflammatory response through inhibiting the formation of inflammatory cytokines and markers of oxidative stress.展开更多
基金Supported by Science and Technology Project from the Beijing Municipal Administration Center of Parks(ZX2018016).
文摘In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of C.coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy C.coggygria in Xunlupo than that in diseased ones in 2018.Verticillium dahliae,which is the causal agent of C.coggygria wilt,was detected in five soil samples.In 2018,the bacterial diversity in the rhizosphere soil of diseased C.coggygria in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy C.coggygria under Platycladus orientalis in Langfengting.
文摘Objective:To assess the analgesic and anti-inflammatory effects of standardized extract of Cotinus coggygria(C.coggygria)in different animal models.Methods:C.coggygria extracts(25,50,and 100 mg/kg)were administered to rats and mice(n=6)during hot plate,tail-flick,acetic acid-induced writhing,and formalin tests to determine its analgesic efficacy.The anti-inflammatory activity of C.coggygria extracts was evaluated by histamine and carrageenan-induced paw edema,cotton pellet-induced granuloma,and acetic acid-induced peritoneal capillary dye leakage tests.Results:C.coggygria extracts(50 and 100 mg/kg)significantly alleviated thermal and chemical-induced pain in rodents(P<0.05).It also demonstrated notable anti-inflammatory properties by mitigating histamine and carrageenan-induced paw edema,granuloma deposits,and vascular permeability(P<0.05).Moreover,C.coggygria extracts remarkably reduced TNF-α,IL-1β,IL-6,COX-2,and oxidative stress in rat paws(P<0.05).Carrageenan-induced histological aberrations in hind paw tissues were effectively(P<0.05)mitigated by treatment with C.coggygria extracts.Conclusions:C.coggygria Scop.extracts show analgesic and anti-inflammatory effects via inhibition of COX-2 and inflammatory and oxidative mediators.
基金supported by the Beijing Science and Technology Planned Project of China (Grant No.D0705002040291)the Beijing Education Committee Research Base Jointly-Funded Project of China+2 种基金the National Project of ScienceTechnology for the 11th Five-Year Plan in China (Grant No. 2006BAD08A1003)the Program for Changjiang Scholars and Innovative Research Team in Universities (PCSIRT0607)
文摘We analyzed the inhibition rates of eight kinds of fungicides to the pathogen of verticillium wilt of smoke tree （Cotinus coggygria） by measuring mycelium growth. Results show that four fungicides （Weijunjing, thiophanate-methyl 70% WP （wettable powder）, carbendazim 50% WP and Junxianwei） have the best antifungal effects. Three fungicides, Weijunjing, carbendazim 50% WP and Junxianwei, were selected to determine the control of verticillium wilt in potted smoke tree seedlings. We found that the relative efficacy of Weijunjing was up to 74%. Based on the results, Weijunjing and carbendazim 50% WP were prepared for field trials. The results indicate that the relative efficacy of Weijunjing （300 times diluted） and carbendazim 50% WP （400 times diluted） were 66.2% and 48.9% in plot 1 and 26.4% and 31.8% in plot 2 after soil disinfection, and were 224% and 61.8% in plot 3 without soil disinfection in 2007. The relative efficacy of Weijunjing and carbendazim 50% WP were 49.6% and 45.4% in plot 2 in 2008.
基金Supported by Scientific Research Fund of Istanbul University(No.TDK-28983)。
文摘Objective:To investigate the therapeutic effects of aqueous infusion of Cotinus coggygria Scop.leaves(CCLAI)on the dextran sulphate sodium(DSS)-induced ulcerative colitis(UC)in mice.Methods:A total of 56 C57BL16J mice were divided into 7 groups,including control,DSS model,CCLAI4,CCLAI6,DSS+CCLAI4,DSS+CCLAI6 and DSS+mesalamine groups(n=8).Induction of UC was conducted by 7-day treatment of 3%DSS in mice.CCLAI in 2 different doses(4%and 6%)or mesalamine(250 mg/kg)was administered to C57BL/6J mice orally for 7 consecutive days.The mice in the control group received only tap water.Clinical parameters,antioxidant and anti-inflammatory activities,and histopathological changes in the colon mucosa were examined.Cell proliferatipn index in colon section was determined by immunonistochemical analysis.Colonic cyclooxygenase(COX)-2 and inducible nitric oxide synthase(i NOS)expression levels were detected by Western blot.Results:CCLAI treatment inhibited DSS-induced colon shortening and significantly improved the Disease Activity Index score(P<0.05 or P<0.01).We also found a significant decrease in myeloperoxidase levels,oxidative stress parameters,and neutrophil infiltration index in the experimental groups receiving CCLAI compared to the control group(all P<0.01).In addition,it was observed that CCLAI administration for 7 days decreased the pro-inflammatory cytokines levels including interleukin(IL)-1β,IL-6,interferon-γ,and tumor necrosis factor-αin the colon tissues of mice receiving DSS(P<0.05).Further,CCLAI treatment reduced the COX-2 and i NOS levels with an accompanying decrease in prostaglandin E2 and nitric oxide levels,respectively.Additionally,CCLAI treatment markedly decreased the histological lesion score,which was consistent with the reduction of the pro-inflammatory cytokine levels.Conclusion:CCLAI treatment significantly improved the DSS-induced UC in mice by diminishing histopathological damage in the colonic mucosa and markedly decreased the inflammatory response through inhibiting the formation of inflammatory cytokines and markers of oxidative stress.
