Wheat is one of the major food crops in the world.Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population.This study...Wheat is one of the major food crops in the world.Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population.This study investigated the role of ABP7,a bHLH transcription factor from maize involved in kernel development,in regulating grain yield-related traits in transgenic wheat.Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression.At the same time,QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type(WT) plants.Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits(e.g.,grain number per spike,grain weight per spike,thousand-grain weight,grain length,and grain width) and increased grain yield per plot,compared to WT plants,whereas line QB205 did not.In addition,total chlorophyll,chlorophyll a,chlorophyll b,and total soluble sugars were largely increased in the flag leaves of both HB123and HB287 transgenic lines compared to the WT.These results strongly suggest that ABP7 positively regulates yieldrelated traits and plot grain yield in transgenic wheat.Consequently,ABP7 can be utilized in wheat breeding for grain yield improvement.展开更多
2014年9月24日,ARM宣布推出最新的32位Cortex-M处理器Cortex-M7,这款处理器相较于目前性能最高的ARM架构微控制器(M C U),可大幅提升两倍的运算及数字信号处理(DSP)性能。ARM Cortex-M7处理器针对高端嵌入式应用,适用于新一代汽车电子...2014年9月24日,ARM宣布推出最新的32位Cortex-M处理器Cortex-M7,这款处理器相较于目前性能最高的ARM架构微控制器(M C U),可大幅提升两倍的运算及数字信号处理(DSP)性能。ARM Cortex-M7处理器针对高端嵌入式应用,适用于新一代汽车电子、连网设备以及智能家居与工业应用。在M7之前,ARM Cortex-M系列内核已推出多款产品:MO、MO+、M3、M4等,在市场上应用极广。展开更多
Genome information from model species such as rice can assist in the cloning of genes in a complex genome, such as maize. Here, we identified a maize ortholog of rice GS5 that contributes to kernel development in maiz...Genome information from model species such as rice can assist in the cloning of genes in a complex genome, such as maize. Here, we identified a maize ortholog of rice GS5 that contributes to kernel development in maize. The genome- wide association analysis of the expression levels of ZmGSs, and 15 of its 26 paralogs, identified a trans-regulator on chromosome 7, which was a BAK1-1ike gene. This gene that we named as ZmBAK1-7 could regulate the expression of ZmGS5 and three of the paralogs. Candidate-gene association analyses revealed that these five genes were associated with maize kernel development-related traits. Linkage analyses also detected that ZINGS5 and ZmI3AK1-7 co-localized with mapped QTLs. A transgenic analysis of ZINGS5 in Arabidopsis thaliana L. showed a significant increase in seed weight and cell number, suggesting that 2mG55 may have a conserved function among different plant species that affects seed development.展开更多
Siberian apricot(Prunus sibirica L.)kernels have a variety of medicinal effects and can be used in the production of Siberian apricot kernel drinks or other foods,such as marzipan or biscuits,but their potential immun...Siberian apricot(Prunus sibirica L.)kernels have a variety of medicinal effects and can be used in the production of Siberian apricot kernel drinks or other foods,such as marzipan or biscuits,but their potential immunoreactivity cannot be ignored.In this research,the hydrolysis effect of several fermented strains on Siberian apricot kernel protein(SAKP)was measured by the degree of hydrolysis,and the content of free amino acids and Lactiplantibacillus plantarum was finally selected to ferment SAKP to reduce its immunoreactivity.SDS‒PAGE and HPLC were used to determine the molecular weight of SAKP and to confirm that the macromolecule was hydrolyzed into small molecules.The fermentation effect was measured by the change in pH and small peptide content in the fermentation process.The optimal fermentation conditions were determined by a single-factor experiment as follows:apricot kernel powder:water=1:20(w/v),inoculation amount of 0.1%(v/w),initial pH of 6.5 for 36 h of fermentation.Atomic force microscopy(AFM)was used to observe the changes in SAKP particle structure after fermentation,and indirect ELISA was used to determine that the immunoreactivity decreased to 29.64%±1.03%after fermentation,which indicated that fermentation reduced the immunoreactivity of SAKP.展开更多
文摘Wheat is one of the major food crops in the world.Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population.This study investigated the role of ABP7,a bHLH transcription factor from maize involved in kernel development,in regulating grain yield-related traits in transgenic wheat.Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression.At the same time,QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type(WT) plants.Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits(e.g.,grain number per spike,grain weight per spike,thousand-grain weight,grain length,and grain width) and increased grain yield per plot,compared to WT plants,whereas line QB205 did not.In addition,total chlorophyll,chlorophyll a,chlorophyll b,and total soluble sugars were largely increased in the flag leaves of both HB123and HB287 transgenic lines compared to the WT.These results strongly suggest that ABP7 positively regulates yieldrelated traits and plot grain yield in transgenic wheat.Consequently,ABP7 can be utilized in wheat breeding for grain yield improvement.
文摘2014年9月24日,ARM宣布推出最新的32位Cortex-M处理器Cortex-M7,这款处理器相较于目前性能最高的ARM架构微控制器(M C U),可大幅提升两倍的运算及数字信号处理(DSP)性能。ARM Cortex-M7处理器针对高端嵌入式应用,适用于新一代汽车电子、连网设备以及智能家居与工业应用。在M7之前,ARM Cortex-M系列内核已推出多款产品:MO、MO+、M3、M4等,在市场上应用极广。
基金supported by the National Natural Science Foundation of China(31222041)the National Hi-Tech Research and Development Program of China (2012AA10A307)
文摘Genome information from model species such as rice can assist in the cloning of genes in a complex genome, such as maize. Here, we identified a maize ortholog of rice GS5 that contributes to kernel development in maize. The genome- wide association analysis of the expression levels of ZmGSs, and 15 of its 26 paralogs, identified a trans-regulator on chromosome 7, which was a BAK1-1ike gene. This gene that we named as ZmBAK1-7 could regulate the expression of ZmGS5 and three of the paralogs. Candidate-gene association analyses revealed that these five genes were associated with maize kernel development-related traits. Linkage analyses also detected that ZINGS5 and ZmI3AK1-7 co-localized with mapped QTLs. A transgenic analysis of ZINGS5 in Arabidopsis thaliana L. showed a significant increase in seed weight and cell number, suggesting that 2mG55 may have a conserved function among different plant species that affects seed development.
基金supported by the National Key R&D Program of China(2017YFC1600902).
文摘Siberian apricot(Prunus sibirica L.)kernels have a variety of medicinal effects and can be used in the production of Siberian apricot kernel drinks or other foods,such as marzipan or biscuits,but their potential immunoreactivity cannot be ignored.In this research,the hydrolysis effect of several fermented strains on Siberian apricot kernel protein(SAKP)was measured by the degree of hydrolysis,and the content of free amino acids and Lactiplantibacillus plantarum was finally selected to ferment SAKP to reduce its immunoreactivity.SDS‒PAGE and HPLC were used to determine the molecular weight of SAKP and to confirm that the macromolecule was hydrolyzed into small molecules.The fermentation effect was measured by the change in pH and small peptide content in the fermentation process.The optimal fermentation conditions were determined by a single-factor experiment as follows:apricot kernel powder:water=1:20(w/v),inoculation amount of 0.1%(v/w),initial pH of 6.5 for 36 h of fermentation.Atomic force microscopy(AFM)was used to observe the changes in SAKP particle structure after fermentation,and indirect ELISA was used to determine that the immunoreactivity decreased to 29.64%±1.03%after fermentation,which indicated that fermentation reduced the immunoreactivity of SAKP.
