Corneal ulceration induced by Staphylococcus pseudintermedius(S.pseudintermedius)is a common clinical eye disease.Antibiotics combined with corneoconjunctival transposition(CCT)or conjunctival autografts(CA)are often ...Corneal ulceration induced by Staphylococcus pseudintermedius(S.pseudintermedius)is a common clinical eye disease.Antibiotics combined with corneoconjunctival transposition(CCT)or conjunctival autografts(CA)are often used,but the recovery characteristics are still unknown.In this experiment,canine corneal ulcer models induced by S.pseudintermedius and treated with levofloxacin eye drops(LED)were created.The models were used to compare the recovery characteristics of CCT and CA,combined with LED,by clinical observation,histopathology,and cytokine expression detected by qRT-PCR analysis.The results showed that the ulcerative cornea with only LED treatment perforated after 48 h.The mRNA expression of TLR2,IL-1β,IL-6,IL-8,and TNF-αgenes was significantly elevated on 14,28,and 35 days after the surgery compared to normal(p<0.01).On day 42,the inflammatory damage had resolved,but the corneal transparency and arrangement of collagen fibrils in the CCT group were higher than those in the CA group.The mRNA expression of EGF,FGF,TGF-β1 and VEGF genes increased significantly(p<0.01),mostly until day 42,proving that CCT and CA surgery contributed to the corneal recovery,and relieved the inflammatory reaction,with the elimination of corneal cicatrices needing a period of reconstruction.Therefore,this study has provided,for the first time,the method for establishing a canine corneal ulcer model induced by S.pseudintermedius.More importantly,the recovery of canine ulcerative corneas with CCT or CA surgery is reported for the first time.展开更多
基金supported financially by 333 High-level Talent Training Project of Jiangsu Province(CN),the Jiangsu Postgraduate Research and Innovation Plan(KYCX21_3273)the 111 Project D18007,Priority Academic Program Development of Jiangsu Higher Education Institution(PAPD),and Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP).
文摘Corneal ulceration induced by Staphylococcus pseudintermedius(S.pseudintermedius)is a common clinical eye disease.Antibiotics combined with corneoconjunctival transposition(CCT)or conjunctival autografts(CA)are often used,but the recovery characteristics are still unknown.In this experiment,canine corneal ulcer models induced by S.pseudintermedius and treated with levofloxacin eye drops(LED)were created.The models were used to compare the recovery characteristics of CCT and CA,combined with LED,by clinical observation,histopathology,and cytokine expression detected by qRT-PCR analysis.The results showed that the ulcerative cornea with only LED treatment perforated after 48 h.The mRNA expression of TLR2,IL-1β,IL-6,IL-8,and TNF-αgenes was significantly elevated on 14,28,and 35 days after the surgery compared to normal(p<0.01).On day 42,the inflammatory damage had resolved,but the corneal transparency and arrangement of collagen fibrils in the CCT group were higher than those in the CA group.The mRNA expression of EGF,FGF,TGF-β1 and VEGF genes increased significantly(p<0.01),mostly until day 42,proving that CCT and CA surgery contributed to the corneal recovery,and relieved the inflammatory reaction,with the elimination of corneal cicatrices needing a period of reconstruction.Therefore,this study has provided,for the first time,the method for establishing a canine corneal ulcer model induced by S.pseudintermedius.More importantly,the recovery of canine ulcerative corneas with CCT or CA surgery is reported for the first time.
