Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Metho...Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.展开更多
目的:观察黄连解毒汤对肺炎衣原体(Cpn)感染后高胆固醇饮食兔血液流变学和动脉粥样硬化的影响。方法:造模前60只新西兰兔经采血检测CpnIgG均为阴性。随机选取8只兔作正常组,其余52只以含2.5 g.kg-1胆固醇的饲料喂养并接种Cpn。经检测血...目的:观察黄连解毒汤对肺炎衣原体(Cpn)感染后高胆固醇饮食兔血液流变学和动脉粥样硬化的影响。方法:造模前60只新西兰兔经采血检测CpnIgG均为阴性。随机选取8只兔作正常组,其余52只以含2.5 g.kg-1胆固醇的饲料喂养并接种Cpn。经检测血清CpnIgG阳性者随机分为4组:黄连解毒汤高、低剂量(3.34,1.67 g.kg-1.d-1)组、阳性药阿奇霉素(20 mg.kg-1.d-1)组和模型组(生理盐水),每组11只,灌胃给药6周;CpnIgG阴性者作高胆固醇组,不予药物干预。治疗结束后采血测定高、中、低全血黏度、血浆黏度和红细胞压积,并计算RBC聚集指数、变形指数和刚性指数;取主动脉弓近左颈总动脉分叉处血管,测定和计算最大血管内膜厚度(M IT)、动脉粥样硬化病变占管周百分比(PLC I)、动脉粥样硬化斑块面积指数(IPA)。结果:高胆固醇组和模型组血液流变学均显著紊乱,主动脉均有典型的动脉粥样硬化病理变化,后者最大M IT(23.65±8.19 vs 12.76±4.06)μm、动脉粥样硬化病变PLC I(41.08±12.51 vs 22.43±9.45)%、动脉粥样硬化斑块IPA(9.57±1.82 vs 2.84±0.25)%显著较前者高(均P<0.01);黄连解毒汤高、低剂量组、阿奇霉素组血液流变学紊乱和动脉粥样硬化病理变化与模型组比较显著改善,其中黄连汤高剂量组M IT(6.45±1.27 vs 23.65±8.19),(P<0.01),PLC I(22.39±6.74 vs41.08±12.51),(P<0.05);IPA(1.44±0.33 vs 9.57±1.82),(P<0.01)显著降低。结论:Cpn感染加重高胆固醇饮食兔血液流变学紊乱和动脉粥样硬化损害,黄连解毒汤可减轻Cpn感染所致高胆固醇饮食兔的血液流变学紊乱和动脉粥样硬化损害。展开更多
文摘Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.
文摘目的:观察黄连解毒汤对肺炎衣原体(Cpn)感染后高胆固醇饮食兔血液流变学和动脉粥样硬化的影响。方法:造模前60只新西兰兔经采血检测CpnIgG均为阴性。随机选取8只兔作正常组,其余52只以含2.5 g.kg-1胆固醇的饲料喂养并接种Cpn。经检测血清CpnIgG阳性者随机分为4组:黄连解毒汤高、低剂量(3.34,1.67 g.kg-1.d-1)组、阳性药阿奇霉素(20 mg.kg-1.d-1)组和模型组(生理盐水),每组11只,灌胃给药6周;CpnIgG阴性者作高胆固醇组,不予药物干预。治疗结束后采血测定高、中、低全血黏度、血浆黏度和红细胞压积,并计算RBC聚集指数、变形指数和刚性指数;取主动脉弓近左颈总动脉分叉处血管,测定和计算最大血管内膜厚度(M IT)、动脉粥样硬化病变占管周百分比(PLC I)、动脉粥样硬化斑块面积指数(IPA)。结果:高胆固醇组和模型组血液流变学均显著紊乱,主动脉均有典型的动脉粥样硬化病理变化,后者最大M IT(23.65±8.19 vs 12.76±4.06)μm、动脉粥样硬化病变PLC I(41.08±12.51 vs 22.43±9.45)%、动脉粥样硬化斑块IPA(9.57±1.82 vs 2.84±0.25)%显著较前者高(均P<0.01);黄连解毒汤高、低剂量组、阿奇霉素组血液流变学紊乱和动脉粥样硬化病理变化与模型组比较显著改善,其中黄连汤高剂量组M IT(6.45±1.27 vs 23.65±8.19),(P<0.01),PLC I(22.39±6.74 vs41.08±12.51),(P<0.05);IPA(1.44±0.33 vs 9.57±1.82),(P<0.01)显著降低。结论:Cpn感染加重高胆固醇饮食兔血液流变学紊乱和动脉粥样硬化损害,黄连解毒汤可减轻Cpn感染所致高胆固醇饮食兔的血液流变学紊乱和动脉粥样硬化损害。
