Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic ...Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.展开更多
[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous ...[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.展开更多
Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reducti...Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.展开更多
Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potent...Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.展开更多
At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degr...At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.展开更多
First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provi...First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.展开更多
To investigate the effects of long term tobacco-rice continuous cropping on soil nutrients and flue-cured tobacco leaf yield and quality, the soils of different con- tinuous cropping years were selected in tobacco-ric...To investigate the effects of long term tobacco-rice continuous cropping on soil nutrients and flue-cured tobacco leaf yield and quality, the soils of different con- tinuous cropping years were selected in tobacco-rice multiple system of Hengyang. Some important indexes, including pH, contents of organic matter and available nu- trients of N P K, contents of medium trace elements of soil samples, and contents of main chemical components of flue-cured tobacco samples were detected for fur- ther analyzing. The results showed that pH value of moderate continuous cropping soil（≤7 years） was more coordinated, in addition, leaf yield and quality of flue-cured tobacco were contained, and the sugar-nicotine ratio and potassium-chlorine ratio were proper. However, under long-term continuous cropping （≥10 years） condition, the soil became acidification, and yield and output value of flue-cured tobacco de- clined. It was suggested that improving soil quality by planting green manure or keeping fallow after continuous cropping 7 years in tobacco-rice system.展开更多
The continuous cropping of flue-cured tobacco in the major producing areas of Henan Province by designing questionnaire, fieldwork, enquiry and phone calls, mails, and other forms, the data were obtained then classifi...The continuous cropping of flue-cured tobacco in the major producing areas of Henan Province by designing questionnaire, fieldwork, enquiry and phone calls, mails, and other forms, the data were obtained then classified and analyzed. The results showed that the main planting areas of flue-cured tobacco were in Nanyang, Xuchang, Luoyang, Sanmenxia, Pingdingshan, and Luohe where were the major producing areas of succession cropping of flue-cured tobacco with percentage of 20%-40% for three years and over three years, and a few places up to 50%. Morbidity was common with wide distribution, and the main diseases were black shank, viral disease, and black rot. It proved that tobacco was not suitable for continuous cropping. Both of tobacco disease and pest rate and mortality significantly increased by more than 40% under continuous cropping for three years and over three years, and the yield and quality of flue-cured tobacco also decreased after continuous cropping. For many places, the tobacco yield started to decrease in the second year of continuous cropping, and after three-year cropping, the yield decreased in the range of 9%-24%. Continuous cropping for a long time would make a serious decline in the quality of tobacco, resulting in non-economic benefits. Therefore, in order to reduce the occurrence of diseases and economic loss in tobacco production, it is recommended to avoid continuous cropping, or to adopt appropriate measures to control tobacco continuous cropping obstacles in the unavoidable case.展开更多
Certain agricultural management practices are known to affect the soil microbial community structure;however,knowledge of the response of the fungal community structure to the long-term continuous cropping and rotatio...Certain agricultural management practices are known to affect the soil microbial community structure;however,knowledge of the response of the fungal community structure to the long-term continuous cropping and rotation of soybean,maize and wheat in the same agroecosystem is limited.We assessed the fungal abundance,composition and diversity among soybean rotation,maize rotation and wheat rotation systems and among long-term continuous cropping systems of soybean,maize and wheat as the effect of crop types on fungal community structure.We compared these fungal parameters of same crop between long-term crop rotation and continuous cropping systems as the effect of cropping systems on fungal community structure.The fungal abundance and composition were measured by quantitative real-time PCR and Illumina MiSeq sequencing.The results revealed that long-term continuous soybean cropping increased the soil fungal abundance compared with soybean rotation,and the fungal abundance was decreased in long-term continuous maize cropping compared with maize rotation.The long-term continuous soybean cropping also exhibited increased soil fungal diversity.The variation in the fungal community structure among the three crops was greater than that between long-term continuous cropping and rotation cropping.Mortierella,Guehomyces and Alternaria were the most important contributors to the dissimilarity of the fungal communities between the continuous cropping and rotation cropping of soybean,maize and wheat.There were 11 potential pathogen and 11 potential biocontrol fungi identified,and the relative abundance of most of the potential pathogenic fungi increased during the long-term continuous cropping of all three crops.The relative abundance of most biocontrol fungi increased in long-term continuous soybean cropping but decreased in long-term continuous maize and wheat cropping.Our results indicate that the response of the soil fungal community structure to long-term continuous cropping varies based upon crop types.展开更多
[Objective] This experiment aimed to investigate the effects of continuous cropping of Panax notoginseng on the properties of rhizosphere soil. [Method] A total of 12 rhizospheres oil samples were collected in the fie...[Objective] This experiment aimed to investigate the effects of continuous cropping of Panax notoginseng on the properties of rhizosphere soil. [Method] A total of 12 rhizospheres oil samples were collected in the fields continuously cropped with P. notoginseng for different years and the soil properties including pH value, contents of available N, available K, available P,total N, total K, total P and organic matter were determined. [Result] With the increase in the number of years of continuous cropping, seven soli indices: soil pH value, organic matter content, total N, total P, total K, available P and available K gradually increased, while available N showed a gradual downward trend. The contents of organic matter content, total N, total P, total K,available P, available K and available N after three years of continuous cropping were increased by 74.93%, 65.85%, 123.82%,18.78%, 341.67%, 120.16% and-32.16%, respectively, indicating that continuous cropping of P. notoginseng resulted in nutrient enrichment in rhizosphere soils. The pH value and available N in IBC(soil inside the border check) and UBC(soil under the border check) were higher than that in BBC(soil beside the border check), suggesting that the soil was gradually alkalized due to the continuous cropping of P. notoginseng. [Conclusion] These results suggest that pH change and nutrient imbalance may be the obstacles to the continuous cropping of P. notoginseng.展开更多
Reports the effects of selenium on lipid peroxidation of soybean under continuous cropping stress.Results of pot culture and plot experiment showed that appropriate amount of Se addition increased the activity of glut...Reports the effects of selenium on lipid peroxidation of soybean under continuous cropping stress.Results of pot culture and plot experiment showed that appropriate amount of Se addition increased the activity of glutathione peroxidase (GSH-Px) significantly,and cleared away the excessive free radicals existing in soybean plant under continuous cropping stress,decreased the concentration of malonaldehyde(MDA),which is the end product of lipid peroxidation,maintained the perfection of chloroplast and mitochondrion structure,enhanced the resistance of continuous cropped soybean,and therefore increased the dry weight of seedlong and yield.展开更多
Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study wa...Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.展开更多
Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequa...Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequately addressed. The objective of the present study was to assess the effects on soil microbial community and yields of waxy maize（Zea mays L.） intercropped with or without residue retention in a continuous broccoli（Brassica oleracea L.） cropping system, i.e., relay intercropping of broccoli and waxy maize（B/M-B）, relay intercropping of broccoli and waxy maize with residue retention（B/MR-B）, and broccoli monoculture（B-B）. The biomass yields of spring and autumn vegetables in B/MR-B were 16.3%–32.5% and 30.1%–46.1% higher than those of B-B,respectively. Autumn vegetable economic yields of B/MR-B were 28.2%–40.3% higher than B-B. The average well color development followed the order： B/MR-B ＆gt; B/M-B ＆gt; B-B. The Shannon index, Simpson index, and Mc Intosh index were higher in B/MR-B than under monoculture. A principal component analysis showed that microbial communities of B/MR-B soils differed from those of B/M-B and B-B soils. Carbon（C） sources utilized by the rhizosphere microorganisms were mainly carbohydrates, carboxylic acids, amino acids, and polymers; however, the C sources for the soil microbial community differed between intercropping and monoculture. The communities from B/MR-B preferred amino acids and polymers. Available nitrogen（N）, potassium（K）, and phosphorus（P） had an obvious impact on soil microbial community. Additionally, the C source utilization by microorganisms was significantly affected by p H and available K and P. Cropping system diversification through relay intercropping and residue retention effectively improved the functional diversity of the soil microbial communities and increased the yields of vegetables.展开更多
In order to find out changes in the main fertility indicators of continuous cropping tobacco fields in Henan Province with years of continuous cropping,5 typical tobacco fields( Jia County,Shaoling District,Fangcheng ...In order to find out changes in the main fertility indicators of continuous cropping tobacco fields in Henan Province with years of continuous cropping,5 typical tobacco fields( Jia County,Shaoling District,Fangcheng County,Xiangcheng County,and Song County) were studied. Indicators in question included activity of main enzymes( sucrase,urease,catalase,and acid phosphatase),p H value,organic matters,total nitrogen,available phosphorus and available potassium. The results indicate that in most tobacco fields,the activity of sucrase and urease increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping; in some fields,it directly declined with increase in years of continuous cropping; the activity of catalase increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping,and it became stable after three years of continuous cropping;generally,the activity of acid phosphatase declined with increase in the years of continuous cropping within 3 years continuous cropping,later,it became stable. Except Fangcheng County,the soil p H value of other districts( counties) generally declined with increase in years of continuous cropping,and it was in the range of 5. 6-6. 8. With the increase in continuous cropping,the soil organic matters( SOM) in tobacco fields of Jia County and Song County declined,changes of SOM in tobacco fields of Fangcheng County and Xiangcheng County were not obvious,and SOM showed the trend of first decline then rise in Shaoling District. In most tobacco fields( Jia County,Xiangcheng County,and Song County),the total nitrogen showed a declining trend; in Shaoling District,it showed a trend of first decline then rise; in Fangcheng County,it showed a rising trend. The available phosphorus firstly slightly declined then rose in tobacco fields of Shaoling District and Song County; it showed a trend of first rise then decline in Jia County; there were no obvious changes in Fangcheng County and Xiangcheng County. Changes in the available potassium were different in tobacco fields. In sum,there are certain rules for changes in soil enzyme activity and nutrient content in tobacco fields in Henan Province,but changes are not completely the same. It is concluded that the site conditions and farming activities exert a certain influence on soil enzyme activity,p H value,organic matters,and nitrogen,phosphorus and potassium content. Therefore,to solve obstacles in continuous cropping of tobacco fields,different regions should take different measures.展开更多
Fxperiments on the effect of soil amendment with rape (Brassica chinensis L.) seed cake, cotton (Gossypium hirsutum L.) seed or sesame (Sesamum indicum L.) seed cake on the growth of cucumber seedlings under a continu...Fxperiments on the effect of soil amendment with rape (Brassica chinensis L.) seed cake, cotton (Gossypium hirsutum L.) seed or sesame (Sesamum indicum L.) seed cake on the growth of cucumber seedlings under a continuous cropping system were conducted in a greenhouse environment. The results indicated that two applications of sesame seed cake (0.1 and 0.5 %, w/w) increased the growth of cucumber, but the rape oil cake showed a negative effect at a rate of 1.5% (w/w). The sesame seed cake was separated into four fractions (Ⅰ, Ⅱ, Ⅲ and Ⅳ) according to the polarity, and all four fractions had a positive effect on the growth of cucumbers under a continuous cropping system. Fraction number Ⅲ was isolated into 25 proportions by silicon column, and only oleic acid, palmitic acid and octadecanoic acid were identified in proportion 10 by GC-MS in which 93.3% was oleic acid and palmitic acid. The oleic acid had a significant and positive effect on cucumber growth under salt stress at the level 30 mmol and showed slight resistance to several pathogenic fungi.展开更多
Continuous cropping in greenhouses can result in root-knot nematode outbreaks resulting from imbalances in the soil nematode community.However,the changes in soil nematode communities in greenhouses with continuous cr...Continuous cropping in greenhouses can result in root-knot nematode outbreaks resulting from imbalances in the soil nematode community.However,the changes in soil nematode communities in greenhouses with continuous crop production are unclear.We compared soil nematode communities in greenhouses after 2 years(2-yr)and 10 years(10-yr)of continuous crop production by 18S rDNA high-throughput sequencing.Compared with the 2-yr greenhouse,soil in the 10-yr greenhouse showed acidification,nutrient accumulation and salinization.Bacterial-feeding nematodes(BF)were dominant in the 2-yr greenhouse over the whole growing season,but plant-parasitic nematodes(PP)were the dominant group in the 10-yr greenhouse during the late growing season.Meloidogyne gradually became the dominant group and had a relative abundance of 70.9%(maximum)in the 10-yr greenhouse.Rhabditidae,with relative abundance ranging from 99.8%to 26.8%,was the predominant group in the 2-year greenhouse.Forβ-diversity,hierarchical clustering analysis,unweighted UniFrac principal component analysis(PCA)and principal co-ordinates analysis(PCoA)all revealed that soil nematode communities in the two types of greenhouses were significantly different.Redundancy analysis(RDA)showed that soil nematode communities in the 10-yr greenhouse were related to high soil organic material,total nitrogen,electrical conductivity and disease index of root-knot nematodes.Fisher’s exact test and Pearson’s correlation coefficients revealed that Meloidogyne contributed to themain differences in soil nematode communities between the two types of greenhouses.Population dynamics of Meloidogyne were divided into dormant phase,low-level increasing phase and exponential phase during the whole season.The soil nematode communities within the 2-yr and 10-yr greenhouses had significant variation and different dynamics.This work contributes to a deeper understanding of changes in the soil nematode community in greenhouses with different continuous cropping duration.展开更多
[Objective] The effects of Dongshengbao liquid organic fertilizer on soil im- provement of continuous cropping cucumber and the growth conditions of the cu- cumber were studied to provide the scientific basis for solv...[Objective] The effects of Dongshengbao liquid organic fertilizer on soil im- provement of continuous cropping cucumber and the growth conditions of the cu- cumber were studied to provide the scientific basis for solving the problem of con- tinuous cropping of vegetable soil. [Method] The soil of continuous cropping was sampled. The soil EC, pH, nutrient contents and cucumber height, dry weight of aboveground part and leaf area etc were studied with the method of potted cucum- ber test. [Result] Soil EC applied by Dongshengbao liquid organic fertilizer reached 0.93 mS/cm, and decreased by 27.91% compared with the contrast. Soil pH reached 5.89, and increased with 0.86% compared with the contrast. Dry weight of aboveground part, plant height and leaf area of cucumber were increased by 283.08%, 242.12% and 254.02% compared with the contrast. The incidence rank of cucumber Didymella bryoniae was below grade 2. Soil EC applied by compound fertilizer reached 1.53 mS/cm, and increased by 18.6% compared with the contrast. Soil pH reached 5.09, and decreased by 12.84% compared with the contrast. Dry weight of aboveground part, plant height and leaf area of cucumber were increased by 32.31%,-37.24% and 26.20% compared with the contrast. The incidence rank of cucumber Didymella bryoniae was above grade 2. [Conclusion] The organic fertilizer could solve the problems of soil salinization and soil acidification of continuous crop- ping, improve the resistance ability of Didymella bryoniae and promote the plant growth.展开更多
In order to actively develop green ecological strawberry, the authors have studied integrated microecological prevention and control technologies of strawberry continuous cropping diseases, including increasing soil b...In order to actively develop green ecological strawberry, the authors have studied integrated microecological prevention and control technologies of strawberry continuous cropping diseases, including increasing soil biomass and solar high temperature disinfection during strawberry greenhouse leisure period in summer, biological fertilizer (agent) treatment before colonization, root irrigation treatment with biocontrol agent, as well as appropriate amount of fulvic acid and cytex after colonization, forming a supporting technical system for the microecological prevention and control of soil-borne diseases in facilities. The demonstration application in production shows that the technology has the functions of restoring and enhancing soil biological fertility, enriching beneficial microbial flora, antagonizing harmful bacteria, significantly promoting the growth and development of strawberry, reducing the incidence of soil-borne diseases, reducing the use of chemical fertilizers and pesticides, promoting the early ripening and high yield of strawberry, significantly improving the quality and flavor of strawberry, reducing the risk of pesticide residues, and boosting consumer confidence, which further improves the economic benefit, social benefit and ecological benefit, with good application prospect in production.展开更多
The aim was to develop green strawberry varieties,control soil-borne diseases from facility strawberry continuous cropping,improve the safety and quality of strawberries and promote the healthy and stable development ...The aim was to develop green strawberry varieties,control soil-borne diseases from facility strawberry continuous cropping,improve the safety and quality of strawberries and promote the healthy and stable development of the strawberry industry. Based on the production practices in recent years,we summarized the physical-biological collaborative prevention and control technology for the disease of facility strawberry continuous cropping facilities,established the technical goals,the main types of soil-borne diseases,and the physical-biological collaborative control prevention and control technologies,mainly including the specific methods and technical points of solar high temperature disinfection technology and biological bacterial fertilizer（ agent） treatment technology.展开更多
Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of conti...Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of continuous melon cropping remain poorly understood.Here,we employed the metagenomic techniques to comparatively investigate the bulk and rhizosphere soil microbial communities of major melon-producing regions(where the duration of continuous melon cropping ranges from 1 to 30 a)in the eastern and southern parts of Xinjiang Uygur Autonomous Region,China.The results showed that soil pH clearly decreased with increasing melon cropping duration,while soil electrical conductivity(EC)and the other soil nutrient indices increased with increasing melon cropping duration(with the exception of AN and TK in the southern melon-producing region).The most dominant bacterial phyla were Proteobacteria and Actinobacteria,and the most abundant fungal phyla were Ascomycota and Mucoromycota.Redundancy analysis(RDA)indicated that soil pH and EC had no significant effects on the bacterial communities.However,after many years of continuous melon cropping in the southern melon-producing region,fungal communities were significantly negatively correlated with soil pH and significantly positively correlated with soil EC(P<0.050).Co-occurrence network analysis showed that continuous melon cropping increased the complexity but decreased the connectivity of the cross-domain microbial networks.Moreover,the enrichment patterns of microorganisms in the main microbial network modules varied significantly with the duration of continuous melon cropping.Based on the analysis of keystone taxa,we found that continuous melon cropping increased some plant pathogens(e.g.,Fusarium and Stagonospora)but decreased beneficial bacteria(e.g.,Mesorhizobium and Pseudoxanthomonas).In conclusion,this study has greatly enhanced the understanding of the effects of continuous melon cropping on alterations in the microbial community structure and ecological networks in Xinjiang.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1700203and 2022YFD1901501)the Tianchi Talent Introduction Program of Xinjiang Autonomous Region,China(2023-“2+5”)the Tingzhou Talent Introduction Program of Changji Autonomous Region,China(2023)。
文摘Crops produced using the practice of continuous cropping can become seriously damaged by plant-parasitic nematodes,an important indicator of continuous cropping obstacles.As a typical and important perennial economic crop,dragon fruit is prone to serious plant-parasitic nematode infestation;however,whether it encounters continuous cropping obstacles remains unclear.Here,we studied plant-parasitic nematodes(Meloidogyne spp.and Tylenchorhynchus sp.)in the soil and roots,soil nematode communities,metabolic footprint,soil integrated fertility,and the yield of intensively planted dragon fruit under non-continuous cropping(Y1)and 3 years(Y3)and 5 years(Y5)of continuous cropping,to determine potential continuous-cropping obstacles and factors that affect the yield of this fruit.The largest numbers of plant-parasitic nematodes in the soil and roots were observed in Y5;the associated yield was reduced,and the dragon fruit was severely stressed.Further analysis of the composition,diversity,and ecological function indices of soil nematodes showed that the soil ecological environment deteriorated after 3 years of continuous cropping,with Y5 having the worst results.Similarly,the soil at Y5 had a significant inhibitory effect on the growth and reproduction of Caenorhabditis elegans.Mantel test analysis and a random forest model showed that soil available phosphorus,soil exchange calcium,and soil nematode abundance and diversity were related significantly to yield.Partial least squares path modeling revealed that soil fertility and soil nematode diversity directly impacts the yield of continuously cropped dragon fruit.In summary,continuous cropping obstacles occurred in Y5 of intensive dragon fruit cultivation,with soil nematode diversity and soil fertility determining the crop's yield.
基金Supported by Agricultural Science and Technology Innovation Fund of Hunan Province(XCNZ[2021]No.15)Loudi Science and Technology Innovation Program(LKF[2022]29)+1 种基金Applied Characteristic Discipline Construction Project of Hunan Province:Plant ProtectionPostgraduate Research and Innovation Project of Hunan University of Humanities,Science and Technology(ZSCX2022Y12).
文摘[Objectives]This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages.[Methods]The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing.Normally-grown first crop P.sibiricum was used as the control group,while continuous cropping plants served as the treatment group.Transcriptomic differences in roots and leaves under different conditions were compared.[Results]The leaf materials of first crop and continuous cropping P.sibiricum(CCLZ vs FCLZ)showed 21916 differentially expressed genes(DEGs),while the root materials of first crop and continuous cropping P.sibiricum(CCRZ vs FCRZ)exhibited 12726 DEGs(the lowest DEG count)(12726).Among them,1896 DEGs were common.GO enrichment analysis revealed that DEGs were mainly enriched in metabolism,cell wall degradation,and pathogen defense.KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways.[Conclusions]This study preliminarily elucidate the regulatory mechanisms in the roots and leaves of continuous cropping P.sibiricum at the molecular level,providing reference for research on its adaptation to continuous cropping.
基金Supported by Key Project of Yunnan Provincial Science and Technology Plan(202202AE090015)Scientific Research Fund of Yunnan Education Department(2024Y742+3 种基金2023Y0863)National Natural Science Foundation of China(42067009)2023 Undergraduate Innovation and Entrepreneurship Training Program of Yunnan Education Department(S202311393044S202311393061).
文摘Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.
基金Supported by National Natural Science Foundation of China(42067009)Scientific Research Project of Yunnan Provincial Department of Education(2024Y742,2023Y0863)+2 种基金Project of Kunming Municipal Commission of Development and Reform(Kunming Spring City Industrial Technology Leading Talents)Science and Technology Major Project of Yunnan Province Science and Technology Department(202202AE090015-02)Yunnan Students’innovation and entrepreneurship training program(S202311393044,S202311393061)。
文摘Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.
基金Supported by Scientific Research Fund of Yunnan Education Department(2024Y742,2023Y0863)National Natural Science Foundation of China(42067009)+1 种基金College Students'Innovative Training Plan Program of Yunnan Education Department in 2023(S202311393044,S202311393061)Key Project of Science and Technology Program of Yunnan Province(202202AE090015).
文摘At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.
文摘First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.
基金Supported by Improvement Mechanism and Application of Soil Quality by Succession Planting and Reclamation in Tobacco Field(14-16ZDAa01)
文摘To investigate the effects of long term tobacco-rice continuous cropping on soil nutrients and flue-cured tobacco leaf yield and quality, the soils of different con- tinuous cropping years were selected in tobacco-rice multiple system of Hengyang. Some important indexes, including pH, contents of organic matter and available nu- trients of N P K, contents of medium trace elements of soil samples, and contents of main chemical components of flue-cured tobacco samples were detected for fur- ther analyzing. The results showed that pH value of moderate continuous cropping soil（≤7 years） was more coordinated, in addition, leaf yield and quality of flue-cured tobacco were contained, and the sugar-nicotine ratio and potassium-chlorine ratio were proper. However, under long-term continuous cropping （≥10 years） condition, the soil became acidification, and yield and output value of flue-cured tobacco de- clined. It was suggested that improving soil quality by planting green manure or keeping fallow after continuous cropping 7 years in tobacco-rice system.
文摘The continuous cropping of flue-cured tobacco in the major producing areas of Henan Province by designing questionnaire, fieldwork, enquiry and phone calls, mails, and other forms, the data were obtained then classified and analyzed. The results showed that the main planting areas of flue-cured tobacco were in Nanyang, Xuchang, Luoyang, Sanmenxia, Pingdingshan, and Luohe where were the major producing areas of succession cropping of flue-cured tobacco with percentage of 20%-40% for three years and over three years, and a few places up to 50%. Morbidity was common with wide distribution, and the main diseases were black shank, viral disease, and black rot. It proved that tobacco was not suitable for continuous cropping. Both of tobacco disease and pest rate and mortality significantly increased by more than 40% under continuous cropping for three years and over three years, and the yield and quality of flue-cured tobacco also decreased after continuous cropping. For many places, the tobacco yield started to decrease in the second year of continuous cropping, and after three-year cropping, the yield decreased in the range of 9%-24%. Continuous cropping for a long time would make a serious decline in the quality of tobacco, resulting in non-economic benefits. Therefore, in order to reduce the occurrence of diseases and economic loss in tobacco production, it is recommended to avoid continuous cropping, or to adopt appropriate measures to control tobacco continuous cropping obstacles in the unavoidable case.
基金funded by the National Key Research and Development Program of China(2016YFD02003009-6 and 2016YFD0300806)the National Natural Science Foundation of China(41771327 and 41571219)the earmarked fund for China Agriculture Research System(CARS04)
文摘Certain agricultural management practices are known to affect the soil microbial community structure;however,knowledge of the response of the fungal community structure to the long-term continuous cropping and rotation of soybean,maize and wheat in the same agroecosystem is limited.We assessed the fungal abundance,composition and diversity among soybean rotation,maize rotation and wheat rotation systems and among long-term continuous cropping systems of soybean,maize and wheat as the effect of crop types on fungal community structure.We compared these fungal parameters of same crop between long-term crop rotation and continuous cropping systems as the effect of cropping systems on fungal community structure.The fungal abundance and composition were measured by quantitative real-time PCR and Illumina MiSeq sequencing.The results revealed that long-term continuous soybean cropping increased the soil fungal abundance compared with soybean rotation,and the fungal abundance was decreased in long-term continuous maize cropping compared with maize rotation.The long-term continuous soybean cropping also exhibited increased soil fungal diversity.The variation in the fungal community structure among the three crops was greater than that between long-term continuous cropping and rotation cropping.Mortierella,Guehomyces and Alternaria were the most important contributors to the dissimilarity of the fungal communities between the continuous cropping and rotation cropping of soybean,maize and wheat.There were 11 potential pathogen and 11 potential biocontrol fungi identified,and the relative abundance of most of the potential pathogenic fungi increased during the long-term continuous cropping of all three crops.The relative abundance of most biocontrol fungi increased in long-term continuous soybean cropping but decreased in long-term continuous maize and wheat cropping.Our results indicate that the response of the soil fungal community structure to long-term continuous cropping varies based upon crop types.
基金Supported by National Natural Science Foundation of China(81102751)the National Key Technology R&D Program of China(2006BAI09B03)
文摘[Objective] This experiment aimed to investigate the effects of continuous cropping of Panax notoginseng on the properties of rhizosphere soil. [Method] A total of 12 rhizospheres oil samples were collected in the fields continuously cropped with P. notoginseng for different years and the soil properties including pH value, contents of available N, available K, available P,total N, total K, total P and organic matter were determined. [Result] With the increase in the number of years of continuous cropping, seven soli indices: soil pH value, organic matter content, total N, total P, total K, available P and available K gradually increased, while available N showed a gradual downward trend. The contents of organic matter content, total N, total P, total K,available P, available K and available N after three years of continuous cropping were increased by 74.93%, 65.85%, 123.82%,18.78%, 341.67%, 120.16% and-32.16%, respectively, indicating that continuous cropping of P. notoginseng resulted in nutrient enrichment in rhizosphere soils. The pH value and available N in IBC(soil inside the border check) and UBC(soil under the border check) were higher than that in BBC(soil beside the border check), suggesting that the soil was gradually alkalized due to the continuous cropping of P. notoginseng. [Conclusion] These results suggest that pH change and nutrient imbalance may be the obstacles to the continuous cropping of P. notoginseng.
基金National Nature Science Foundation projectgrant No:39870 4 70 Natural Scieuce Foundation of Heilongjiang Province
文摘Reports the effects of selenium on lipid peroxidation of soybean under continuous cropping stress.Results of pot culture and plot experiment showed that appropriate amount of Se addition increased the activity of glutathione peroxidase (GSH-Px) significantly,and cleared away the excessive free radicals existing in soybean plant under continuous cropping stress,decreased the concentration of malonaldehyde(MDA),which is the end product of lipid peroxidation,maintained the perfection of chloroplast and mitochondrion structure,enhanced the resistance of continuous cropped soybean,and therefore increased the dry weight of seedlong and yield.
基金supported by the National Key R&D Program of China(2018YFD1000902)the Natural Science Foundation of Shandong Province(ZR2021QC163).
文摘Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.
基金financially supported by the Special Research Funding for Public Benefit Industries (Agriculture) of China (No. 201503121)the Key Projects in the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period, China (No. 2012BAD14B07)
文摘Intercropping and residue retention contribute to high yield and quality of crops. However, their coupled effects on rhizospheric microbial communities under a continuous vegetable cropping system have not been adequately addressed. The objective of the present study was to assess the effects on soil microbial community and yields of waxy maize（Zea mays L.） intercropped with or without residue retention in a continuous broccoli（Brassica oleracea L.） cropping system, i.e., relay intercropping of broccoli and waxy maize（B/M-B）, relay intercropping of broccoli and waxy maize with residue retention（B/MR-B）, and broccoli monoculture（B-B）. The biomass yields of spring and autumn vegetables in B/MR-B were 16.3%–32.5% and 30.1%–46.1% higher than those of B-B,respectively. Autumn vegetable economic yields of B/MR-B were 28.2%–40.3% higher than B-B. The average well color development followed the order： B/MR-B ＆gt; B/M-B ＆gt; B-B. The Shannon index, Simpson index, and Mc Intosh index were higher in B/MR-B than under monoculture. A principal component analysis showed that microbial communities of B/MR-B soils differed from those of B/M-B and B-B soils. Carbon（C） sources utilized by the rhizosphere microorganisms were mainly carbohydrates, carboxylic acids, amino acids, and polymers; however, the C sources for the soil microbial community differed between intercropping and monoculture. The communities from B/MR-B preferred amino acids and polymers. Available nitrogen（N）, potassium（K）, and phosphorus（P） had an obvious impact on soil microbial community. Additionally, the C source utilization by microorganisms was significantly affected by p H and available K and P. Cropping system diversification through relay intercropping and residue retention effectively improved the functional diversity of the soil microbial communities and increased the yields of vegetables.
基金Supported by Key Scientific and Technological Plan Project of Henan Tobacco Company(HYKJM201304)
文摘In order to find out changes in the main fertility indicators of continuous cropping tobacco fields in Henan Province with years of continuous cropping,5 typical tobacco fields( Jia County,Shaoling District,Fangcheng County,Xiangcheng County,and Song County) were studied. Indicators in question included activity of main enzymes( sucrase,urease,catalase,and acid phosphatase),p H value,organic matters,total nitrogen,available phosphorus and available potassium. The results indicate that in most tobacco fields,the activity of sucrase and urease increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping; in some fields,it directly declined with increase in years of continuous cropping; the activity of catalase increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping,and it became stable after three years of continuous cropping;generally,the activity of acid phosphatase declined with increase in the years of continuous cropping within 3 years continuous cropping,later,it became stable. Except Fangcheng County,the soil p H value of other districts( counties) generally declined with increase in years of continuous cropping,and it was in the range of 5. 6-6. 8. With the increase in continuous cropping,the soil organic matters( SOM) in tobacco fields of Jia County and Song County declined,changes of SOM in tobacco fields of Fangcheng County and Xiangcheng County were not obvious,and SOM showed the trend of first decline then rise in Shaoling District. In most tobacco fields( Jia County,Xiangcheng County,and Song County),the total nitrogen showed a declining trend; in Shaoling District,it showed a trend of first decline then rise; in Fangcheng County,it showed a rising trend. The available phosphorus firstly slightly declined then rose in tobacco fields of Shaoling District and Song County; it showed a trend of first rise then decline in Jia County; there were no obvious changes in Fangcheng County and Xiangcheng County. Changes in the available potassium were different in tobacco fields. In sum,there are certain rules for changes in soil enzyme activity and nutrient content in tobacco fields in Henan Province,but changes are not completely the same. It is concluded that the site conditions and farming activities exert a certain influence on soil enzyme activity,p H value,organic matters,and nitrogen,phosphorus and potassium content. Therefore,to solve obstacles in continuous cropping of tobacco fields,different regions should take different measures.
文摘Fxperiments on the effect of soil amendment with rape (Brassica chinensis L.) seed cake, cotton (Gossypium hirsutum L.) seed or sesame (Sesamum indicum L.) seed cake on the growth of cucumber seedlings under a continuous cropping system were conducted in a greenhouse environment. The results indicated that two applications of sesame seed cake (0.1 and 0.5 %, w/w) increased the growth of cucumber, but the rape oil cake showed a negative effect at a rate of 1.5% (w/w). The sesame seed cake was separated into four fractions (Ⅰ, Ⅱ, Ⅲ and Ⅳ) according to the polarity, and all four fractions had a positive effect on the growth of cucumbers under a continuous cropping system. Fraction number Ⅲ was isolated into 25 proportions by silicon column, and only oleic acid, palmitic acid and octadecanoic acid were identified in proportion 10 by GC-MS in which 93.3% was oleic acid and palmitic acid. The oleic acid had a significant and positive effect on cucumber growth under salt stress at the level 30 mmol and showed slight resistance to several pathogenic fungi.
基金the National Natural Science Funds of China(Grant No.31101418)National Science Foundation of Henan(Grant No.182300410020)+2 种基金National Key Research&Development(R&D)plan(Grant No.2017ZY060063)the Plant Protection Key Discipline of Henan Province,China Agriculture Research System(Grant No.CARS-25)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Continuous cropping in greenhouses can result in root-knot nematode outbreaks resulting from imbalances in the soil nematode community.However,the changes in soil nematode communities in greenhouses with continuous crop production are unclear.We compared soil nematode communities in greenhouses after 2 years(2-yr)and 10 years(10-yr)of continuous crop production by 18S rDNA high-throughput sequencing.Compared with the 2-yr greenhouse,soil in the 10-yr greenhouse showed acidification,nutrient accumulation and salinization.Bacterial-feeding nematodes(BF)were dominant in the 2-yr greenhouse over the whole growing season,but plant-parasitic nematodes(PP)were the dominant group in the 10-yr greenhouse during the late growing season.Meloidogyne gradually became the dominant group and had a relative abundance of 70.9%(maximum)in the 10-yr greenhouse.Rhabditidae,with relative abundance ranging from 99.8%to 26.8%,was the predominant group in the 2-year greenhouse.Forβ-diversity,hierarchical clustering analysis,unweighted UniFrac principal component analysis(PCA)and principal co-ordinates analysis(PCoA)all revealed that soil nematode communities in the two types of greenhouses were significantly different.Redundancy analysis(RDA)showed that soil nematode communities in the 10-yr greenhouse were related to high soil organic material,total nitrogen,electrical conductivity and disease index of root-knot nematodes.Fisher’s exact test and Pearson’s correlation coefficients revealed that Meloidogyne contributed to themain differences in soil nematode communities between the two types of greenhouses.Population dynamics of Meloidogyne were divided into dormant phase,low-level increasing phase and exponential phase during the whole season.The soil nematode communities within the 2-yr and 10-yr greenhouses had significant variation and different dynamics.This work contributes to a deeper understanding of changes in the soil nematode community in greenhouses with different continuous cropping duration.
基金Supported by Jiangsu Agricultural Technological Promotion Program(TG(14)007)Changshu Scientific and Technological Development Plan(CN[2014]01,CN2014C10)+1 种基金Jiangsu Agricultural Three New Program(SXGC[2014]081)China Spark Program(S2015C100048)~~
文摘[Objective] The effects of Dongshengbao liquid organic fertilizer on soil im- provement of continuous cropping cucumber and the growth conditions of the cu- cumber were studied to provide the scientific basis for solving the problem of con- tinuous cropping of vegetable soil. [Method] The soil of continuous cropping was sampled. The soil EC, pH, nutrient contents and cucumber height, dry weight of aboveground part and leaf area etc were studied with the method of potted cucum- ber test. [Result] Soil EC applied by Dongshengbao liquid organic fertilizer reached 0.93 mS/cm, and decreased by 27.91% compared with the contrast. Soil pH reached 5.89, and increased with 0.86% compared with the contrast. Dry weight of aboveground part, plant height and leaf area of cucumber were increased by 283.08%, 242.12% and 254.02% compared with the contrast. The incidence rank of cucumber Didymella bryoniae was below grade 2. Soil EC applied by compound fertilizer reached 1.53 mS/cm, and increased by 18.6% compared with the contrast. Soil pH reached 5.09, and decreased by 12.84% compared with the contrast. Dry weight of aboveground part, plant height and leaf area of cucumber were increased by 32.31%,-37.24% and 26.20% compared with the contrast. The incidence rank of cucumber Didymella bryoniae was above grade 2. [Conclusion] The organic fertilizer could solve the problems of soil salinization and soil acidification of continuous crop- ping, improve the resistance ability of Didymella bryoniae and promote the plant growth.
基金Supported by Key Research and Development Program of Zhenjiang City(NY2020017)Independent Innovation Project of Agricultural Science and Technology of Jiangsu Province(CX[21]2019).
文摘In order to actively develop green ecological strawberry, the authors have studied integrated microecological prevention and control technologies of strawberry continuous cropping diseases, including increasing soil biomass and solar high temperature disinfection during strawberry greenhouse leisure period in summer, biological fertilizer (agent) treatment before colonization, root irrigation treatment with biocontrol agent, as well as appropriate amount of fulvic acid and cytex after colonization, forming a supporting technical system for the microecological prevention and control of soil-borne diseases in facilities. The demonstration application in production shows that the technology has the functions of restoring and enhancing soil biological fertility, enriching beneficial microbial flora, antagonizing harmful bacteria, significantly promoting the growth and development of strawberry, reducing the incidence of soil-borne diseases, reducing the use of chemical fertilizers and pesticides, promoting the early ripening and high yield of strawberry, significantly improving the quality and flavor of strawberry, reducing the risk of pesticide residues, and boosting consumer confidence, which further improves the economic benefit, social benefit and ecological benefit, with good application prospect in production.
基金Supported by the Comprehensive Treatment Technology Research and Development for Greenhouse Strawberry Successive Cropping Obstacles,the Agricultural Science and Technology Support Program of Jurong City(NY2016745091)the Integrated Demonstration of Green Control Technologies for Strawberry Pests and Diseases,the Demonstration and Promotion Project of Shanghai Municipal Agricultural Commission[Hunongketui Zi(2015)No.2-7]
文摘The aim was to develop green strawberry varieties,control soil-borne diseases from facility strawberry continuous cropping,improve the safety and quality of strawberries and promote the healthy and stable development of the strawberry industry. Based on the production practices in recent years,we summarized the physical-biological collaborative prevention and control technology for the disease of facility strawberry continuous cropping facilities,established the technical goals,the main types of soil-borne diseases,and the physical-biological collaborative control prevention and control technologies,mainly including the specific methods and technical points of solar high temperature disinfection technology and biological bacterial fertilizer（ agent） treatment technology.
基金funded by the Major Science and Technology Projects of Xinjiang Uygur Autonomous Region(2022A02007-4)the Xinjiang Uygur Autonomous Region Natural Science Foundation Youth Project(2024D01B31)the Graduate Student Research Innovation Project of Xinjiang Agricultural University(XJAUGRI2024033).
文摘Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of continuous melon cropping remain poorly understood.Here,we employed the metagenomic techniques to comparatively investigate the bulk and rhizosphere soil microbial communities of major melon-producing regions(where the duration of continuous melon cropping ranges from 1 to 30 a)in the eastern and southern parts of Xinjiang Uygur Autonomous Region,China.The results showed that soil pH clearly decreased with increasing melon cropping duration,while soil electrical conductivity(EC)and the other soil nutrient indices increased with increasing melon cropping duration(with the exception of AN and TK in the southern melon-producing region).The most dominant bacterial phyla were Proteobacteria and Actinobacteria,and the most abundant fungal phyla were Ascomycota and Mucoromycota.Redundancy analysis(RDA)indicated that soil pH and EC had no significant effects on the bacterial communities.However,after many years of continuous melon cropping in the southern melon-producing region,fungal communities were significantly negatively correlated with soil pH and significantly positively correlated with soil EC(P<0.050).Co-occurrence network analysis showed that continuous melon cropping increased the complexity but decreased the connectivity of the cross-domain microbial networks.Moreover,the enrichment patterns of microorganisms in the main microbial network modules varied significantly with the duration of continuous melon cropping.Based on the analysis of keystone taxa,we found that continuous melon cropping increased some plant pathogens(e.g.,Fusarium and Stagonospora)but decreased beneficial bacteria(e.g.,Mesorhizobium and Pseudoxanthomonas).In conclusion,this study has greatly enhanced the understanding of the effects of continuous melon cropping on alterations in the microbial community structure and ecological networks in Xinjiang.
