Osteocytes reside as three-dimensionally（3D） networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of os...Osteocytes reside as three-dimensionally（3D） networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of osteocytes have been challenging because：（1） current cell lines do not sufficiently represent the phenotypic features of mature osteocytes and（2） primary cells rapidly differentiate to osteoblasts upon isolation. In this study, we used a 3D perfusion culture approach to：（1） construct the 3D cellular network of primary murine osteocytes by biomimetic assembly with microbeads and（2） reproduce ex vivo the phenotype of primary murine osteocytes, for the first time to our best knowledge. In order to enable 3D construction with a sufficient number of viable cells, we used a proliferated osteoblastic population of healthy cells outgrown from digested bone chips. The diameter of microbeads was controlled to：（1） distribute and entrap cells within the interstitial spaces between the microbeads and（2） maintain average cell-to-cell distance to be about 19 mm. The entrapped cells formed a 3D cellular network by extending and connecting their processes through openings between the microbeads. Also, with increasing culture time, the entrapped cells exhibited the characteristic gene expressions（SOST and FGF23） and nonproliferative behavior of mature osteocytes. In contrast, 2D-cultured cells continued their osteoblastic differentiation and proliferation. This 3D biomimetic approach is expected to provide a new means of：（1） studying flow-induced shear stress on the mechanotransduction function of primary osteocytes,（2） studying physiological functions of 3D-networked osteocytes with in vitro convenience,and（3） developing clinically relevant human bone disease models.展开更多
The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achiev...The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achieve using satellite remote sensing.Considering the convenient,facilitative,and flexible characteristics of UAV(unmanned air vehicle)remote sensing tech-nology,this study selects a campus as a typical research area and uses the Pegasus D2000 equipped with a D-MSPC2000 multi-spectral camera and a CAM3000 aerial camera to acquire oblique images and multi-spectral data.Using professional software,including Context Capture,ENVI,and ArcGIS,a 3D(three-dimensional)campus model,a digital orthophoto map,and multi-spectral remote sensing map drawing are realized,and the geometric accuracy of typical feature selection is evaluated.Based on a quantitative remote sensing model,the campus ecological environment assessment is performed from the perspectives of vegetation and water body.The results presented in this study could be of great significance to the scientific management and sustainable development of regional natural resources.展开更多
1. The problems on hybrid network combining TD-SCDMA 1) Hybrid network is imperative in China At present, there are several major wireless networks in China, namely, GSM network of China Mobile, GSM network and CDMA n...1. The problems on hybrid network combining TD-SCDMA 1) Hybrid network is imperative in China At present, there are several major wireless networks in China, namely, GSM network of China Mobile, GSM network and CDMA network of China Unicom, PHS network of China Telecom and China Netcom, and therefore the seamless transition from the existent wireless network to 3G network is an inevitable展开更多
In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was ...In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.展开更多
The purpose of this paper is to present a general universal formula for <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;&...The purpose of this paper is to present a general universal formula for <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate survival functions for arbitrary <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 2, 3, <span style="font-family:;" "=""><span style="font-family:Verdana;">...<span style="font-family:Verdana;">, given all the univariate marginal survival functions. This universal form of <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate probability distributions was obtained by means of “dependence functions” named “joiners” in the text. These joiners determine all the involved stochastic dependencies between the underlying random variables. However, in order that the presented formula (the form) represents a legitimate survival function, some necessary and sufficient conditions for the joiners had to be found. Basically, finding those conditions is the main task of this paper. This task was successfully performed for the case <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 2 and the main results for the case <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 3 were formulated as Theorem 1 and Theorem 2 in Section 4. Nevertheless, the hypothetical conditions valid for the general <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> ≥ 4 case were also formulated in Section 3 as the (very convincing) Hypothesis. As for the sufficient conditions for both the <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:;" "=""><span style="font-family:Verdana;"> = 3 and <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> ≥ 4 cases, the full generality was not achieved since two restrictions were imposed. Firstly, we limited ourselves to the, defined in the text, “continuous cases” (when the corresponding joint density exists and is continuous), and secondly we consider positive stochastic dependencies only. Nevertheless, the class of the <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate distributions which can be constructed is very wide. The presented method of construction by means of joiners can be considered competitive to the <span style="font-family:Verdana;"><span style="font-family:Verdana;">copula<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> methodology. As it is suggested in the paper the possibility of building a common theory of both copulae and joiners is quite possible, and the joiners may play the role of tools within the theory of copulae, and vice versa copulae may, for example, be used for finding proper joiners. Another independent feature of the joiners methodology is the possibility of constructing many new stochastic processes including stationary and Markovian.展开更多
基金the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health (1R21AR065032 to W.Y.L and J.Z.)the National Science Foundation (DMR 1409779 to W.Y.L and J.Z.)
文摘Osteocytes reside as three-dimensionally（3D） networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of osteocytes have been challenging because：（1） current cell lines do not sufficiently represent the phenotypic features of mature osteocytes and（2） primary cells rapidly differentiate to osteoblasts upon isolation. In this study, we used a 3D perfusion culture approach to：（1） construct the 3D cellular network of primary murine osteocytes by biomimetic assembly with microbeads and（2） reproduce ex vivo the phenotype of primary murine osteocytes, for the first time to our best knowledge. In order to enable 3D construction with a sufficient number of viable cells, we used a proliferated osteoblastic population of healthy cells outgrown from digested bone chips. The diameter of microbeads was controlled to：（1） distribute and entrap cells within the interstitial spaces between the microbeads and（2） maintain average cell-to-cell distance to be about 19 mm. The entrapped cells formed a 3D cellular network by extending and connecting their processes through openings between the microbeads. Also, with increasing culture time, the entrapped cells exhibited the characteristic gene expressions（SOST and FGF23） and nonproliferative behavior of mature osteocytes. In contrast, 2D-cultured cells continued their osteoblastic differentiation and proliferation. This 3D biomimetic approach is expected to provide a new means of：（1） studying flow-induced shear stress on the mechanotransduction function of primary osteocytes,（2） studying physiological functions of 3D-networked osteocytes with in vitro convenience,and（3） developing clinically relevant human bone disease models.
基金supported by the National Natural Science Foundation of China (Grant No.42171311)the Open Fund of State Key Laboratory of Remote Sensing Science (Grant No.OFSLRSS202218)+1 种基金the Key Research and Development Program of the Hainan Province,China (Grant No.ZDYF2021SHFZ105)the Training Program of Excellent Master Thesis of Zhejiang Ocean University.
文摘The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achieve using satellite remote sensing.Considering the convenient,facilitative,and flexible characteristics of UAV(unmanned air vehicle)remote sensing tech-nology,this study selects a campus as a typical research area and uses the Pegasus D2000 equipped with a D-MSPC2000 multi-spectral camera and a CAM3000 aerial camera to acquire oblique images and multi-spectral data.Using professional software,including Context Capture,ENVI,and ArcGIS,a 3D(three-dimensional)campus model,a digital orthophoto map,and multi-spectral remote sensing map drawing are realized,and the geometric accuracy of typical feature selection is evaluated.Based on a quantitative remote sensing model,the campus ecological environment assessment is performed from the perspectives of vegetation and water body.The results presented in this study could be of great significance to the scientific management and sustainable development of regional natural resources.
文摘1. The problems on hybrid network combining TD-SCDMA 1) Hybrid network is imperative in China At present, there are several major wireless networks in China, namely, GSM network of China Mobile, GSM network and CDMA network of China Unicom, PHS network of China Telecom and China Netcom, and therefore the seamless transition from the existent wireless network to 3G network is an inevitable
文摘In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.
文摘The purpose of this paper is to present a general universal formula for <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate survival functions for arbitrary <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 2, 3, <span style="font-family:;" "=""><span style="font-family:Verdana;">...<span style="font-family:Verdana;">, given all the univariate marginal survival functions. This universal form of <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate probability distributions was obtained by means of “dependence functions” named “joiners” in the text. These joiners determine all the involved stochastic dependencies between the underlying random variables. However, in order that the presented formula (the form) represents a legitimate survival function, some necessary and sufficient conditions for the joiners had to be found. Basically, finding those conditions is the main task of this paper. This task was successfully performed for the case <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 2 and the main results for the case <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> = 3 were formulated as Theorem 1 and Theorem 2 in Section 4. Nevertheless, the hypothetical conditions valid for the general <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> ≥ 4 case were also formulated in Section 3 as the (very convincing) Hypothesis. As for the sufficient conditions for both the <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:;" "=""><span style="font-family:Verdana;"> = 3 and <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;"> ≥ 4 cases, the full generality was not achieved since two restrictions were imposed. Firstly, we limited ourselves to the, defined in the text, “continuous cases” (when the corresponding joint density exists and is continuous), and secondly we consider positive stochastic dependencies only. Nevertheless, the class of the <span style="font-family:Verdana;"><span style="font-family:Verdana;">k<span style="font-family:Verdana;"><span style="font-family:Verdana;">-variate distributions which can be constructed is very wide. The presented method of construction by means of joiners can be considered competitive to the <span style="font-family:Verdana;"><span style="font-family:Verdana;">copula<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> methodology. As it is suggested in the paper the possibility of building a common theory of both copulae and joiners is quite possible, and the joiners may play the role of tools within the theory of copulae, and vice versa copulae may, for example, be used for finding proper joiners. Another independent feature of the joiners methodology is the possibility of constructing many new stochastic processes including stationary and Markovian.
