Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the...Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the pepper industry by affecting the planting period,growth conditions,and disease susceptibility.The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance.In order to explore the upstream regulatory mechanism of CaABI3/VP1-1,a high-quality standardized yeast hybrid library was successfully constructed for yeast one-,two-,and threehybrid screening using pepper‘ZHC2’as the experimental material,with a library recombinant efficiency of up to 100%.The length of inserted fragments varied from 650 to 5000 bp,the library titer was 5.18×10^(6)colony-forming units(CFU)·mL-1,and the library capacity was 1.04×10^(7)CFU of cDNA inserts.The recombinant bait plasmid was used to successfully identify 78 different proteins through the yeast one-hybrid system,including one transcription factor within the ethylene-responsive factor family and the other within the growth-regulating factor family.The interaction happened between LOC124895848 and CaABI3/VP1-1 promoter by point-to-point yeast one-hybrid experiment.The expression level of the 12 selected protein-coding genes was then evaluated by quantitative real-time polymerase chain reaction.Results indicated the protein coding genes showed different responses to waterlogging stress and that the activity of the CaABI3/VP1-1 promoter could be inhibited or activated by up-regulating or down-regulating gene expression,respectively.The identification of these proteins interacting with the promoter provides a new perspective for understanding the gene regulatory network of hot pepper operating under waterlogging stress and provides theoretical support for further analysis of the complex regulatory relationship between transcription factors and promoters.展开更多
In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,t...In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,the ballistic interaction process was successfully simulated based on the Tuler eButcher and GISSMO coupling failure model.The simulated conoidal fracture morphology was in good agreement with the three-dimensional industrial-computed-tomography image.Further,three main damage zones(zones I,II,and III)were identified besides the crater area,which are located respectively near the crater area,at the back of the target plate,and directly below the crater area.Under the high-speed-impact conditions,in zone II,cracks began to form at the end of the period of crack formation in zone I,but crack formation in zone III started before the end of crack formation in zone II.Further,the damage mechanism differed for different stress states.The microcracks in zone I were formed both by void connection and shear deformation.In the formation of zone I,the stress triaxiality ranged from2.0 to1.0,and the shear failure mechanism played a dominant role.The microcracks in zone II showed the combined features of shear deformation and void connection,and during the formation process,the stress triaxiality was between 0 and 0.5 with a mixed failure mode.Further,the microcracks in zone III showed obvious characteristics of void connection caused by local melting.During the zone III formation,the triaxiality was 1.0e1.9,and the ductile fracture mechanism was dominant,which also reflects the phenomenon of spallation.展开更多
In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with ...In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with (α, β)-metrics are given. Last, the Gauss curvature of the conoid in the 3-dimension Randers-Minkowski space is studied.展开更多
目的:使用三维可变翻转角快速自旋回波(three⁃dimensional sampling perfection with application optimized contrast using different flip angle evolution,3D⁃SPACE)序列联合压缩感知(compressed sensing,CS)技术,在健康人群中定量...目的:使用三维可变翻转角快速自旋回波(three⁃dimensional sampling perfection with application optimized contrast using different flip angle evolution,3D⁃SPACE)序列联合压缩感知(compressed sensing,CS)技术,在健康人群中定量测量喙锁韧带的两个组成部分,斜方韧带与锥状韧带各自的长度、附着点宽度、附着点位置等解剖学参数。方法:收集无肩部外伤行肩关节磁共振(magnetic resonance imaging,MRI)检查的志愿者64例,进行冠状位T1加权像(T1⁃weighted image,T1WI)3D CS⁃SPACE序列的扫描。两名放射科医师分别对图像进行多平面重建,在斜冠状位图像上测量斜方韧带与锥状韧带的长度、锁骨上附着处宽度以及附着处中心点距锁骨外侧缘的距离,在斜矢状位图像上测量两者在喙突上附着处宽度,在斜轴位图像上测量斜方韧带与锥状韧带在喙突附着处中心点距喙突外侧缘的距离以及喙突的大小。当两名医师测量结果具有一致性时,取平均值用于统计学分析。按性别分组,比较各参数组间一致性,并将喙锁韧带各解剖定量数据与年龄、喙突大小进行相关性分析。结果:斜方韧带长度、在锁骨及喙突上的附着处宽度、附着处中心点距锁骨、喙突外侧缘的距离以及锥状韧带附着处中心点距锁骨、喙突外侧缘的距离在男女组间的差异均有统计学意义(P均<0.05)。年龄与各解剖参数间均不具有相关性(P均>0.05)。除锥状韧带长度外,其余斜方韧带及锥状韧带解剖学定量参数均与喙突大小具有不同程度的相关性(P均<0.05)。结论:3D CS⁃SPACE序列能够清晰显示斜方韧带与锥状韧带的解剖学结构,通过其获得斜方韧带与锥状韧带的定量解剖学参数,为肩锁关节损伤患者的个体化手术治疗提供依据,有利于减少并发症。展开更多
In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH an...In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.展开更多
Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were a...Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.展开更多
Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activ...Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.展开更多
A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual lab...A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual laborer. The dislocation is classified according to Rockwood. Types Ⅰ?and Ⅱ are treated nonoperatively, while types Ⅳ, Ⅴ and Ⅵ are generally treated operatively. Controversy exists regarding the optimal treatment of type Ⅲ dislocations in the high-demand patient. Recent evidence suggests that these should be treated nonoperatively initially. Classic surgical techniques were associated with high complication rates, including recurrent dislocations and hardware breakage. In recent years, many new techniques have been introduced in order to improve the outcomes. Arthroscopic reconstruction or repair techniques have promising short-term results. This article aims to provide a current concepts review on the treatment of AC dislocations with emphasis on recent developments.展开更多
基金funded by the National Natural Science Foundation of China(grant no.32260760)the Science and Technology Program of Guizhou Province(grant no.20201Z002)the Platform Construction Project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province(Qianjiaoji[2022]No.040).
文摘Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the pepper industry by affecting the planting period,growth conditions,and disease susceptibility.The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance.In order to explore the upstream regulatory mechanism of CaABI3/VP1-1,a high-quality standardized yeast hybrid library was successfully constructed for yeast one-,two-,and threehybrid screening using pepper‘ZHC2’as the experimental material,with a library recombinant efficiency of up to 100%.The length of inserted fragments varied from 650 to 5000 bp,the library titer was 5.18×10^(6)colony-forming units(CFU)·mL-1,and the library capacity was 1.04×10^(7)CFU of cDNA inserts.The recombinant bait plasmid was used to successfully identify 78 different proteins through the yeast one-hybrid system,including one transcription factor within the ethylene-responsive factor family and the other within the growth-regulating factor family.The interaction happened between LOC124895848 and CaABI3/VP1-1 promoter by point-to-point yeast one-hybrid experiment.The expression level of the 12 selected protein-coding genes was then evaluated by quantitative real-time polymerase chain reaction.Results indicated the protein coding genes showed different responses to waterlogging stress and that the activity of the CaABI3/VP1-1 promoter could be inhibited or activated by up-regulating or down-regulating gene expression,respectively.The identification of these proteins interacting with the promoter provides a new perspective for understanding the gene regulatory network of hot pepper operating under waterlogging stress and provides theoretical support for further analysis of the complex regulatory relationship between transcription factors and promoters.
基金This work was supported by the National Natural Science Foundation of China(Grant No.51571031).
文摘In order to understand the mechanism of conoidal fracture damage caused by a high-speed fragmentsimulating projectile in titanium alloy layer of a composite armor plate composed of titanium-and aluminum-alloy layers,the ballistic interaction process was successfully simulated based on the Tuler eButcher and GISSMO coupling failure model.The simulated conoidal fracture morphology was in good agreement with the three-dimensional industrial-computed-tomography image.Further,three main damage zones(zones I,II,and III)were identified besides the crater area,which are located respectively near the crater area,at the back of the target plate,and directly below the crater area.Under the high-speed-impact conditions,in zone II,cracks began to form at the end of the period of crack formation in zone I,but crack formation in zone III started before the end of crack formation in zone II.Further,the damage mechanism differed for different stress states.The microcracks in zone I were formed both by void connection and shear deformation.In the formation of zone I,the stress triaxiality ranged from2.0 to1.0,and the shear failure mechanism played a dominant role.The microcracks in zone II showed the combined features of shear deformation and void connection,and during the formation process,the stress triaxiality was between 0 and 0.5 with a mixed failure mode.Further,the microcracks in zone III showed obvious characteristics of void connection caused by local melting.During the zone III formation,the triaxiality was 1.0e1.9,and the ductile fracture mechanism was dominant,which also reflects the phenomenon of spallation.
文摘In this paper, minimal submanifolds in Finsler spaces with (α, β)-metrics are studied. Especially, helicoids are also minimal in (α, β)-Minkowski spaces. Then the minimal surfaces of conoid in Finsler spaces with (α, β)-metrics are given. Last, the Gauss curvature of the conoid in the 3-dimension Randers-Minkowski space is studied.
文摘In this study,the biosorption of Malachite green(MG)onto Turbinaria conoides,brown marine algae,was studied with respect to initial pH,temperature,initial dye concentration,and sorbent dosage.The optimum initial pH and temperature values for MG removal were found to be 8.0 and 30℃,respectively.Sorbent dosage was found to strongly influence the removal of MG.Equilibrium studies were carried out to test the validity of the Langmuir(q_(max)=66.6 mg/g and b=0.526 mL mol/L)and the Freundlich(n=1.826 and K=3.751 mg/g)isotherms.The kinetic studies indicated the validity of the pseudo first-order and second-order equation.
基金Supported by Ministry of Education(MOE)of Malaysia and Research Intensive Faculty(RIF)of University of Technology MARA(UiTM)Malaysia[Grant No.600-RMI/DANA 5/3/RIF(185/2012)].
文摘Objective:To evaluate the in vivo acute toxicity and antioxidant activity of the marine endophytic fungus extract S2 isolated from Turbinaria conoides.Methods:Two doses(100 mg/kg and 400 mg/kg)of the S2 extract were administered to rats orally for acute toxicity and antioxidant test.The body weight,relative weight of six organs,haematological,biochemical and antioxidant properties were investigated on Day 14.Results:A single oral dose treatment did not cause any mortality or observable adverse effects in rats.No significant variations in the body and organ weights between the control and the treated groups were observed.Heamatological analysis and clinical blood chemistry also did not reveal any toxic effects of the extract.The total white blood cell count and haemoglobin levels were increased.The levels of total serum cholesterol in males treated with 100 and 400 mg/kg were significantly(P<0.05)decreased(1.28 and 1.34 mmol/L respectively)compared to control(1.55 mmol/L)rats.Pathologically,neither gross abnormalities nor histopathological changes were observed.This study showed strong evidence of the non-toxic effects of S2 extract.Furthermore the extract exhibited significant(P<0.05)antioxidant activity through increased levels of superoxide dismutase and glutathione peroxidase enzy mes in serum,liver and kidney.Conclusions:The research findings from the present study showed the potential of marine natural products particularly in Malaysia as a source of bioactive compounds.Marine endophytic fungi as a potential source of anticancer drugs with great potential as they are potent yet safe,thus deserving further extensive investigation.
基金Supported by University Grants Commission,New Delhi.India
文摘Objective:To evaluate the antimicrobial activity of Turbinaria conoides(T.conoides).Padina gymnospora(P.gymnospora) and Sargassum tenerrimum against human bacterial and fungal pathogens.Methods:The antimicrobial activities of the extracts against various organisms were tested by using disc diffusion method.Results:The methanol extract showed the better result than the other extracts.Whereas,the strong antibacterial inhibition was noted in methanol extracts of P.gymnospora against Bacillus subtilus(26.33±1.86) and the mild inhibition of ethanol extracts from T.conoides against Klebsiella pneumoniae(2.33±0.51).Acetone extraction of P. gymnospora had strong antifungal inhibition against Cryptococcus neoformans(23.00±1.78), and acetone extract of T.conoides had mild inhibition against Aspergillus niger(3.00±0.89). Conclusions:The seven different solvent extracts of seaweeds used in the present study have shown significant bacterial action.Further,a detailed study on the principle compound in the seaweeds which is responsible for antimicrobial activity is still needed and it can be achieved by using advanced separation techniques.
文摘A direct force on the superior aspect of the shoulder may cause acromioclavicular(AC) dislocation or separation. Severe dislocations can lead to chronic impairment, especially in the athlete and high-demand manual laborer. The dislocation is classified according to Rockwood. Types Ⅰ?and Ⅱ are treated nonoperatively, while types Ⅳ, Ⅴ and Ⅵ are generally treated operatively. Controversy exists regarding the optimal treatment of type Ⅲ dislocations in the high-demand patient. Recent evidence suggests that these should be treated nonoperatively initially. Classic surgical techniques were associated with high complication rates, including recurrent dislocations and hardware breakage. In recent years, many new techniques have been introduced in order to improve the outcomes. Arthroscopic reconstruction or repair techniques have promising short-term results. This article aims to provide a current concepts review on the treatment of AC dislocations with emphasis on recent developments.
