Objective:To delineate the frequency of occurrence of bla_(CTX-M),bla_(TEM),and bla_(SHV) in Enterobacteriaceae from North-Indian tertiary hospital.Methods:A random collection of a subset of 45 Escherichia coli(E.coli...Objective:To delineate the frequency of occurrence of bla_(CTX-M),bla_(TEM),and bla_(SHV) in Enterobacteriaceae from North-Indian tertiary hospital.Methods:A random collection of a subset of 45 Escherichia coli(E.coli) and 28 Klebsiella pneumoniae(K.pneumoniae) that was resistant to a third generation cephalosporin and obtained during 2007-2008 was selected for detailed screening for bla_(CTX-M),bla_(TEM),and bla_(SHV) by monoplex PCRs.The isolates demonstrating the presence of bla_(CTX-M) alleles were characterized for the specific CTX-M-genogroup by using a multiplex PCR.Results:Resistance to cefoperazone,ceftazidime,ceftriaxone,cefotaxime, cefoxitin and piperacillin was 100%each in K.pneumoniae isolates,whereas these resistancerates for E.coli isolates were 93.1%,83.8%,91.9%,93.6%,97.3%and 97.1%,respectively. Concomitant resistance to aminoglycosides,quinolones and aztreonam was also noticed.Presence of any of the bla genes(bla_(CTX-M),bla_(TEM),and bla_(SHV)) was noticed in a total of 28(38.4%) isolates of the 73 isolates studied.Many isolates demonstrated occurrence of these genes in various combinations.bla_(CTX-M),bla_(TEM),and bla_(SHV) were noticed in 28.8%,10.9%and 13.7%isolates, respectively.Multiplex PCR in bla_(CTX-M) harboring isolates demonstrated the presence of CTX-MGenogroup -1 and sequencing for the specific CTX-M-type revealed presence of CTX-M-15 type. RAPD typing showed wide diversity in isolates.Conclusions:This is amongst the premier report describing the simultaneous occurrence of blo_(TEM),bla_(SHV),and bla_(ampC) in Indian Enterobacteriaceae and that wider dissemination of these genes,as demonstrated by diversity of isolates,raises concern and emphasizes a need for extensive search for the presence of these gene pools in Indian subcontinent.展开更多
基金Department of Science & Technology,Ministry of Science & Technology,Government of India,for the award of Young Scientist Project(SR/FT/ L-111/2006)
文摘Objective:To delineate the frequency of occurrence of bla_(CTX-M),bla_(TEM),and bla_(SHV) in Enterobacteriaceae from North-Indian tertiary hospital.Methods:A random collection of a subset of 45 Escherichia coli(E.coli) and 28 Klebsiella pneumoniae(K.pneumoniae) that was resistant to a third generation cephalosporin and obtained during 2007-2008 was selected for detailed screening for bla_(CTX-M),bla_(TEM),and bla_(SHV) by monoplex PCRs.The isolates demonstrating the presence of bla_(CTX-M) alleles were characterized for the specific CTX-M-genogroup by using a multiplex PCR.Results:Resistance to cefoperazone,ceftazidime,ceftriaxone,cefotaxime, cefoxitin and piperacillin was 100%each in K.pneumoniae isolates,whereas these resistancerates for E.coli isolates were 93.1%,83.8%,91.9%,93.6%,97.3%and 97.1%,respectively. Concomitant resistance to aminoglycosides,quinolones and aztreonam was also noticed.Presence of any of the bla genes(bla_(CTX-M),bla_(TEM),and bla_(SHV)) was noticed in a total of 28(38.4%) isolates of the 73 isolates studied.Many isolates demonstrated occurrence of these genes in various combinations.bla_(CTX-M),bla_(TEM),and bla_(SHV) were noticed in 28.8%,10.9%and 13.7%isolates, respectively.Multiplex PCR in bla_(CTX-M) harboring isolates demonstrated the presence of CTX-MGenogroup -1 and sequencing for the specific CTX-M-type revealed presence of CTX-M-15 type. RAPD typing showed wide diversity in isolates.Conclusions:This is amongst the premier report describing the simultaneous occurrence of blo_(TEM),bla_(SHV),and bla_(ampC) in Indian Enterobacteriaceae and that wider dissemination of these genes,as demonstrated by diversity of isolates,raises concern and emphasizes a need for extensive search for the presence of these gene pools in Indian subcontinent.
