Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: b...Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.展开更多
目的研究复方蜥蜴散不同微粒组合剂对胃癌前病变大鼠Stat3通路Stat3、Bcl-2蛋白表达的影响,探讨复方蜥蜴散不同微粒组合剂对胃癌前病变抗凋亡蛋白的调控机制。方法将120只SD雄性大鼠随机分成空白对照组、复方蜥蜴散不同微粒组合剂80目...目的研究复方蜥蜴散不同微粒组合剂对胃癌前病变大鼠Stat3通路Stat3、Bcl-2蛋白表达的影响,探讨复方蜥蜴散不同微粒组合剂对胃癌前病变抗凋亡蛋白的调控机制。方法将120只SD雄性大鼠随机分成空白对照组、复方蜥蜴散不同微粒组合剂80目治疗组、100目治疗组、80目和100目等量混合治疗组、胃癌前病变模型对照组、维酶素治疗组,除空白对照组外,均用N-甲基-N'-硝基-N-亚硝基胍、饥饱失常及情绪刺激综合因素8周制备胃癌前病变大鼠模型。分别用复方蜥蜴散不同微粒组合剂80目、100目、80目100目等量混合糊剂混悬液、生理盐水、维酶素治疗,观察大鼠胃黏膜病理组织学变化及应用免疫组化法检测胃体组织Stat3、Bcl-2蛋白表达情况。结果通过Image-Pro Plus 6.0图像分析系统对免疫组化图像进行光密度的测算,复方蜥蜴散不同微粒组合剂80目治疗组、100目治疗组、80目和100目等量混合治疗组Stat3、Bcl-2蛋白阳性表达率低于维霉素治疗组(P<0.05),其中以复方蜥蜴散80目100目等量混合治疗组差异最显著(P<0.01)。结论复方蜥蜴散不同微粒组合剂能部分逆转胃黏膜病理变化,降低Stat3、Bcl-2蛋白的表达,促进细胞凋亡,抑制细胞过度增殖,恢复细胞增殖与凋亡的平衡,揭示了复方蜥蜴散不同微粒组合剂可能是通过对抗凋亡蛋白的调控实现治疗胃癌前病变的作用机制的。展开更多
目的:观察以终末脱氧核糖核酸转移酶介导的原位缺口末端标记(terminal deoxynucleotidyl transferase-me-diated dUTP nick end labeling,TUNEL)技术检测复方蜥蜴散治疗后对CAG大鼠模型胃黏膜细胞凋亡的影响。方法:将90只SD雄性大鼠随...目的:观察以终末脱氧核糖核酸转移酶介导的原位缺口末端标记(terminal deoxynucleotidyl transferase-me-diated dUTP nick end labeling,TUNEL)技术检测复方蜥蜴散治疗后对CAG大鼠模型胃黏膜细胞凋亡的影响。方法:将90只SD雄性大鼠随机分为正常组,模型组,复方蜥蜴散大、中、小剂量组及维酶素组。除正常组以外,均采取55℃热盐水、2%水杨酸、20mmol/L脱氧胆酸钠3个致萎缩因素配合饥饱失常造成大鼠CAG模型,分别运用复方蜥蜴散大、中、小剂量及维酶素治疗,记录各组大鼠体重变化,以TUNEL法测定各组大鼠胃黏膜细胞凋亡率。结果:造模后大鼠体重增加缓慢,与正常组大鼠相比体重明显减轻(P<0.01)。治疗4周后,复方蜥蜴散大剂量组较模型组体重略有增加(P<0.05)。与正常组相比,模型组凋亡细胞指数显著升高(P<0.01);与模型组相比,各治疗组胃黏膜细胞凋亡率显著升高(P<0.01);与维酶素组相比,复方蜥蜴散治疗各组胃黏膜细胞凋亡率显著升高(P<0.05);与复方蜥蜴散小剂量组比较,复方蜥蜴散大剂量组胃黏膜细胞凋亡率显著升高(P<0.01)。结论:复方蜥蜴散治疗后可使CAG模型大鼠体重增加,并且可促进胃黏膜病变细胞凋亡,阻断CAG病变细胞的增殖,从而逆转胃黏膜组织萎缩,防止CAG的发生。展开更多
文摘Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.
文摘目的研究复方蜥蜴散不同微粒组合剂对胃癌前病变大鼠Stat3通路Stat3、Bcl-2蛋白表达的影响,探讨复方蜥蜴散不同微粒组合剂对胃癌前病变抗凋亡蛋白的调控机制。方法将120只SD雄性大鼠随机分成空白对照组、复方蜥蜴散不同微粒组合剂80目治疗组、100目治疗组、80目和100目等量混合治疗组、胃癌前病变模型对照组、维酶素治疗组,除空白对照组外,均用N-甲基-N'-硝基-N-亚硝基胍、饥饱失常及情绪刺激综合因素8周制备胃癌前病变大鼠模型。分别用复方蜥蜴散不同微粒组合剂80目、100目、80目100目等量混合糊剂混悬液、生理盐水、维酶素治疗,观察大鼠胃黏膜病理组织学变化及应用免疫组化法检测胃体组织Stat3、Bcl-2蛋白表达情况。结果通过Image-Pro Plus 6.0图像分析系统对免疫组化图像进行光密度的测算,复方蜥蜴散不同微粒组合剂80目治疗组、100目治疗组、80目和100目等量混合治疗组Stat3、Bcl-2蛋白阳性表达率低于维霉素治疗组(P<0.05),其中以复方蜥蜴散80目100目等量混合治疗组差异最显著(P<0.01)。结论复方蜥蜴散不同微粒组合剂能部分逆转胃黏膜病理变化,降低Stat3、Bcl-2蛋白的表达,促进细胞凋亡,抑制细胞过度增殖,恢复细胞增殖与凋亡的平衡,揭示了复方蜥蜴散不同微粒组合剂可能是通过对抗凋亡蛋白的调控实现治疗胃癌前病变的作用机制的。
文摘目的:观察以终末脱氧核糖核酸转移酶介导的原位缺口末端标记(terminal deoxynucleotidyl transferase-me-diated dUTP nick end labeling,TUNEL)技术检测复方蜥蜴散治疗后对CAG大鼠模型胃黏膜细胞凋亡的影响。方法:将90只SD雄性大鼠随机分为正常组,模型组,复方蜥蜴散大、中、小剂量组及维酶素组。除正常组以外,均采取55℃热盐水、2%水杨酸、20mmol/L脱氧胆酸钠3个致萎缩因素配合饥饱失常造成大鼠CAG模型,分别运用复方蜥蜴散大、中、小剂量及维酶素治疗,记录各组大鼠体重变化,以TUNEL法测定各组大鼠胃黏膜细胞凋亡率。结果:造模后大鼠体重增加缓慢,与正常组大鼠相比体重明显减轻(P<0.01)。治疗4周后,复方蜥蜴散大剂量组较模型组体重略有增加(P<0.05)。与正常组相比,模型组凋亡细胞指数显著升高(P<0.01);与模型组相比,各治疗组胃黏膜细胞凋亡率显著升高(P<0.01);与维酶素组相比,复方蜥蜴散治疗各组胃黏膜细胞凋亡率显著升高(P<0.05);与复方蜥蜴散小剂量组比较,复方蜥蜴散大剂量组胃黏膜细胞凋亡率显著升高(P<0.01)。结论:复方蜥蜴散治疗后可使CAG模型大鼠体重增加,并且可促进胃黏膜病变细胞凋亡,阻断CAG病变细胞的增殖,从而逆转胃黏膜组织萎缩,防止CAG的发生。
