Differential gene expression profiles in Balb/cJ mouse model of acute hepatic failure infected with MHV-3 virus intervened by anti-hepatic failure compound （AHFC） and the changes of cytokines regulated by genes were...Differential gene expression profiles in Balb/cJ mouse model of acute hepatic failure infected with MHV-3 virus intervened by anti-hepatic failure compound （AHFC） and the changes of cytokines regulated by genes were investigated. The Balb/cj mice were divided into AHFC-intervened group and control group randomly. Acute hepatic failure model of Balb/cJ mice infected with MHV-3 virus was established. The survival rate in the two groups was observed. It was found that the survival rate in the AHFC-intervened group and control group was 90% and 50% respectively 48 h after intraperitoneal injection of MHV-3 （P〈0.05）. Before and after the experiment, the cytokines in peripheral blood of the survival mice were determined, and RNA was extracted from survival mouse liver tissue for the analysis of the differential gene expression by a 36 kb mouse oligonuleotide DNA array. In all the genes of microarray there were 332 genes expressed differently in the two groups, in which 234 genes were up-regulated and 78 genes down-regulated. Through clustering analysis, the differential expression of immune related genes, including TNF receptor superfamily, Kctd9, Bcl-2, Fgl2, IL-8, IL-6, IFN-7, TNF-α etc. might be related with the curative effectiveness of AHFC. It was suggested that AHFC can balance the immune state of mouse model of acute hepatic failure infected with MHV-3 virus mainly through regulating the expression of immune related genes, decrease the immune damage and inhibit liver cell apoptosis of mouse acute hepatic failure model obviously so as to increase the survival rate of mouse models of acute hepatic failure.展开更多
Axle-box bearings are crucial components of high-speed trains and operate in challenging conditions.As service mileage increases,these bearings are susceptible to various failures,posing a safety risk to high-speed tr...Axle-box bearings are crucial components of high-speed trains and operate in challenging conditions.As service mileage increases,these bearings are susceptible to various failures,posing a safety risk to high-speed train operations.Thus,it is crucial to examine the deployment methods of axle-box bearings.A dynamic model of axle-box bearings for high-speed trains with compound faults is constructed by setting up separate faults in two rows of double-row tapered roller bearings based on a single-fault model.The model's high accuracy in expressing compound faults is verified through corresponding experimental results.Then,the frequency domain diagram of system vibration response under varying rotational speed conditions is obtained,and the amplitude corresponding to the single frequency is extracted and analyzed to identify the optimal rotational speed band for composite fault diagnosis.Finally,the optimal speed band is analyzed under different faults,different load sizes,and different composite fault types.It can be concluded that the determination of the optimal speed band is solely influenced by the composite fault type and is independent of the fault and load sizes.Finally,it is concluded that the energy proportion of faults in different positions changes periodically with the change in speed,and this phenomenon is not affected by the fault sizes or load magnitude.展开更多
基金This project was supported by a grant from Hubei Province Natural Sciences Foundation of China (No2007ABA114)
文摘Differential gene expression profiles in Balb/cJ mouse model of acute hepatic failure infected with MHV-3 virus intervened by anti-hepatic failure compound （AHFC） and the changes of cytokines regulated by genes were investigated. The Balb/cj mice were divided into AHFC-intervened group and control group randomly. Acute hepatic failure model of Balb/cJ mice infected with MHV-3 virus was established. The survival rate in the two groups was observed. It was found that the survival rate in the AHFC-intervened group and control group was 90% and 50% respectively 48 h after intraperitoneal injection of MHV-3 （P〈0.05）. Before and after the experiment, the cytokines in peripheral blood of the survival mice were determined, and RNA was extracted from survival mouse liver tissue for the analysis of the differential gene expression by a 36 kb mouse oligonuleotide DNA array. In all the genes of microarray there were 332 genes expressed differently in the two groups, in which 234 genes were up-regulated and 78 genes down-regulated. Through clustering analysis, the differential expression of immune related genes, including TNF receptor superfamily, Kctd9, Bcl-2, Fgl2, IL-8, IL-6, IFN-7, TNF-α etc. might be related with the curative effectiveness of AHFC. It was suggested that AHFC can balance the immune state of mouse model of acute hepatic failure infected with MHV-3 virus mainly through regulating the expression of immune related genes, decrease the immune damage and inhibit liver cell apoptosis of mouse acute hepatic failure model obviously so as to increase the survival rate of mouse models of acute hepatic failure.
基金National Natural Science Foundation of China,Grant/Award Numbers:12032017,12002221Key Scientific Research Projects of China Railway Group,Grant/Award Number:N2021J032+1 种基金S&T Program of Hebei,Grant/Award Number:21567622HNational Scholarship Council of China。
文摘Axle-box bearings are crucial components of high-speed trains and operate in challenging conditions.As service mileage increases,these bearings are susceptible to various failures,posing a safety risk to high-speed train operations.Thus,it is crucial to examine the deployment methods of axle-box bearings.A dynamic model of axle-box bearings for high-speed trains with compound faults is constructed by setting up separate faults in two rows of double-row tapered roller bearings based on a single-fault model.The model's high accuracy in expressing compound faults is verified through corresponding experimental results.Then,the frequency domain diagram of system vibration response under varying rotational speed conditions is obtained,and the amplitude corresponding to the single frequency is extracted and analyzed to identify the optimal rotational speed band for composite fault diagnosis.Finally,the optimal speed band is analyzed under different faults,different load sizes,and different composite fault types.It can be concluded that the determination of the optimal speed band is solely influenced by the composite fault type and is independent of the fault and load sizes.Finally,it is concluded that the energy proportion of faults in different positions changes periodically with the change in speed,and this phenomenon is not affected by the fault sizes or load magnitude.
