After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after cul...After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.展开更多
[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship ...[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship was found in the range of 0.10 -0.50 μg, and the average recovery rate was 100.59% （RSD = 1.38% ）. [ Conclusion] The method is simple, rapid and reproducible, and could be used as a method for quality control of Compound Danshen Tablets.展开更多
Objective:Left ventricular remodeling induced by myocardial ischemia/reperfusion injury(MI/RI)is a common cardiac dysfunction.Accumulating evidence has demonstrated that autophagy plays a vital role in protecting agai...Objective:Left ventricular remodeling induced by myocardial ischemia/reperfusion injury(MI/RI)is a common cardiac dysfunction.Accumulating evidence has demonstrated that autophagy plays a vital role in protecting against ventricular remodeling.This study aims to investigate the performance of Compound Danshen Tablets(CDT)in rescuing ventricular remodeling and whether autophagy as the potential mechanism.Methods:The left anterior descending arteries of rats were temporarily ligated for 30 min to construct the MI/RI model.Ventricular remodeling was induced by reperfusion for 28 d,during which the MI/RI rats were administered CDT(300 mg/kg and 600 mg/kg),atorvastatin(2 mg/kg),and diltiazem(16 mg/kg).Cardiac function and structure were examined by echocardiography.Immunohistochemistry,Masson's trichrome staining,and hematoxylin-eosin(HE)staining were utilized to assess the fibrosis and histological alterations in the heart tissue.The expression of autophagy-related proteins was detected using Western blotting.Results:CDT attenuated the cardiac dysfunction,structural changes,histopathological changes and fibrosis induced by MI/RI.CDT significantly enhanced the level of Beclin1 and microtubule-associated protein1 light chain 3 beta(LC3β),and reduced p62 levels in MI/RI rats.Moreover,CDT significantly increased the phosphorylation of adenosine monophosphate-activated protein kinase(AMPK)and inhibited mammalian target of rapamycin(mTOR)phosphorylation.Conclusion:CDT ameliorated MI/RI-induced ventricular remodeling by activating autophagy and improving autophagic flux via the AMPK/mTOR signaling pathway.展开更多
基金supported by the Bureau of Traditional Chinese Medicine of Guangdong Province, No. 2010463the National Science and Technology"12~(th) Five-years"Major Special-purpose Foundation,No.2011ZX09201-201-01
文摘After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.
文摘[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship was found in the range of 0.10 -0.50 μg, and the average recovery rate was 100.59% （RSD = 1.38% ）. [ Conclusion] The method is simple, rapid and reproducible, and could be used as a method for quality control of Compound Danshen Tablets.
基金supported by Guangdong Province Key Field R&D Program Project(No.2020B111111002)the National Natural Science Foundation of China(No.U1812403-5-381891012)。
文摘Objective:Left ventricular remodeling induced by myocardial ischemia/reperfusion injury(MI/RI)is a common cardiac dysfunction.Accumulating evidence has demonstrated that autophagy plays a vital role in protecting against ventricular remodeling.This study aims to investigate the performance of Compound Danshen Tablets(CDT)in rescuing ventricular remodeling and whether autophagy as the potential mechanism.Methods:The left anterior descending arteries of rats were temporarily ligated for 30 min to construct the MI/RI model.Ventricular remodeling was induced by reperfusion for 28 d,during which the MI/RI rats were administered CDT(300 mg/kg and 600 mg/kg),atorvastatin(2 mg/kg),and diltiazem(16 mg/kg).Cardiac function and structure were examined by echocardiography.Immunohistochemistry,Masson's trichrome staining,and hematoxylin-eosin(HE)staining were utilized to assess the fibrosis and histological alterations in the heart tissue.The expression of autophagy-related proteins was detected using Western blotting.Results:CDT attenuated the cardiac dysfunction,structural changes,histopathological changes and fibrosis induced by MI/RI.CDT significantly enhanced the level of Beclin1 and microtubule-associated protein1 light chain 3 beta(LC3β),and reduced p62 levels in MI/RI rats.Moreover,CDT significantly increased the phosphorylation of adenosine monophosphate-activated protein kinase(AMPK)and inhibited mammalian target of rapamycin(mTOR)phosphorylation.Conclusion:CDT ameliorated MI/RI-induced ventricular remodeling by activating autophagy and improving autophagic flux via the AMPK/mTOR signaling pathway.
