Potentilla anserine L.,a traditional functional food in Xizang,is known for its immunomodulatory and antioxidant properties.Previous studies have shown the pharmacological potential of glycolipids,however,their glycos...Potentilla anserine L.,a traditional functional food in Xizang,is known for its immunomodulatory and antioxidant properties.Previous studies have shown the pharmacological potential of glycolipids,however,their glycosidic instability has limited further exploration into their mechanisms.This study aimed to isolate and characterize novel glycolipids from Potentilla anserine rhizomes,evaluate their anti-inflammatory and hepatoprotective ac-tivities,and elucidate the underlying mechanisms.Glycolipids were extracted and purified using column chro-matography and HPLC,and their structures were identified through NMR and MS analysis.Network pharmacology was applied to predict core targets and pathways involved in inflammation and hepatic injury.In vitro assays assessed the inhibition of 15-lipoxygenase,NO production,and cytokine secretion in LPS-stimulated RAW 264.7 cells,alongside hepatoprotective effects in H₂O₂-induced LO_(2) cell injury models.Molecular docking simulated the interactions between active glycolipids and core target proteins,while structure-activity rela-tionship(SAR)analysis examined the correlation between structural features and bioactivity.As a result,eight novel glycolipids(poteriglycolipids A-H)and ten known compounds were identified.Compounds 2-4 and 9-13 significantly inhibited 15-lipoxygenase and NO production,while compounds 4,12,and 13 suppressed cytokine secretion.Compounds 1-7 showed significant hepatoprotective effects by reducing oxidative damage.Molecular docking validated the strong binding affinities,and SAR analysis confirmed structural factors influencing bioactivity.In conclusion,this study revealed novel glycolipids with robust anti-inflammatory and hep-atoprotective properties,offering mechanistic insights and laying the foundation for the development of glycolipid-based functional foods and therapeutics.展开更多
文摘Potentilla anserine L.,a traditional functional food in Xizang,is known for its immunomodulatory and antioxidant properties.Previous studies have shown the pharmacological potential of glycolipids,however,their glycosidic instability has limited further exploration into their mechanisms.This study aimed to isolate and characterize novel glycolipids from Potentilla anserine rhizomes,evaluate their anti-inflammatory and hepatoprotective ac-tivities,and elucidate the underlying mechanisms.Glycolipids were extracted and purified using column chro-matography and HPLC,and their structures were identified through NMR and MS analysis.Network pharmacology was applied to predict core targets and pathways involved in inflammation and hepatic injury.In vitro assays assessed the inhibition of 15-lipoxygenase,NO production,and cytokine secretion in LPS-stimulated RAW 264.7 cells,alongside hepatoprotective effects in H₂O₂-induced LO_(2) cell injury models.Molecular docking simulated the interactions between active glycolipids and core target proteins,while structure-activity rela-tionship(SAR)analysis examined the correlation between structural features and bioactivity.As a result,eight novel glycolipids(poteriglycolipids A-H)and ten known compounds were identified.Compounds 2-4 and 9-13 significantly inhibited 15-lipoxygenase and NO production,while compounds 4,12,and 13 suppressed cytokine secretion.Compounds 1-7 showed significant hepatoprotective effects by reducing oxidative damage.Molecular docking validated the strong binding affinities,and SAR analysis confirmed structural factors influencing bioactivity.In conclusion,this study revealed novel glycolipids with robust anti-inflammatory and hep-atoprotective properties,offering mechanistic insights and laying the foundation for the development of glycolipid-based functional foods and therapeutics.
