Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological...Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological functions and,importantly,disease-related roles of the prion protein(PrP),a relatively broadly expressed membrane-anchored glycoprotein with high levels in several cell types of the nervous and immune system and with well-established key roles in different progressive and fatal neurodegenerative protein misfolding diseases(proteopathies).展开更多
1 Introduction Single-cell analyses have rapidly emerged as powerful tools for studying cellular heterogeneity,gene regulation,and complex biological processes[1].A key technology of singlecell analyses is single-cell...1 Introduction Single-cell analyses have rapidly emerged as powerful tools for studying cellular heterogeneity,gene regulation,and complex biological processes[1].A key technology of singlecell analyses is single-cell chromatin accessibility sequencing(scCAS)which enables the analysis of chromatin accessibility at the resolution of individual cells[2],providing unprecedented insights into gene regulation,cellular differentiation,and disease mechanisms by profiling key regulatory regions such as transcription factor binding sites,enhancers,and promoters.展开更多
基金supported by the CJD Foundation,USA,the Alzheimer Forschung Initiative(AFI)e.V.,Germany,and Werner-Otto-Stiftung,Germany(all to HCA),ChinaScholarship Council(grant#202108080249 to FS)Deutsche Forschungsgemeinschaft(DFG)CRC877“Proteolysis as a regulatory event in pathophysiology”(project A12 to MG),Slovene Research and InnovationAgency(grant number P4-0176 to VCS).
文摘Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological functions and,importantly,disease-related roles of the prion protein(PrP),a relatively broadly expressed membrane-anchored glycoprotein with high levels in several cell types of the nervous and immune system and with well-established key roles in different progressive and fatal neurodegenerative protein misfolding diseases(proteopathies).
基金supported by the National Natural Science Foundation of China(Grant Nos.62473212,62203236)the Young Elite Scientists Sponsorship Program by CAST[2023QNRC001].
文摘1 Introduction Single-cell analyses have rapidly emerged as powerful tools for studying cellular heterogeneity,gene regulation,and complex biological processes[1].A key technology of singlecell analyses is single-cell chromatin accessibility sequencing(scCAS)which enables the analysis of chromatin accessibility at the resolution of individual cells[2],providing unprecedented insights into gene regulation,cellular differentiation,and disease mechanisms by profiling key regulatory regions such as transcription factor binding sites,enhancers,and promoters.
