Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed t...Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.展开更多
In this paper we present how an serious security relevant event can be taken care of duing Olympic Games.Remote healthcare treatment will be given to injured persons during nuclear radiological biological attacks or i...In this paper we present how an serious security relevant event can be taken care of duing Olympic Games.Remote healthcare treatment will be given to injured persons during nuclear radiological biological attacks or in the case of physical disasters. The "Emergency-112" wireless telemedicine platform provides the hardware and software inrastructures in order to cope with the most extreme scenarios.A fully autonomous mobile system interconnects a group of injured persons with the expert medical institution.The unlimited capabilities of the system allows the Emergency-112 platform to be sed for rescue and surveillance operations regardless the terrain or the weather conditions.A dynamic hybrid system monitors the available spectrum and actively switches between different telecommunication access gateways.展开更多
基金the support of the National Natural Science Foundation of China (Grant No.82272503)Natural Science Foundation of Zhejiang Province (Grant No. LQN25H060006)
文摘Exosomes have shown good potential in ischemic injury disease treatments.However,evidence about their effect and molecular mechanisms in osteonecrosis of femoral head(ONFH)treatment is still limited.Here,we revealed the cell biology characters of ONFH osteonecrosis area bone tissue in single cell scale and thus identified a novel ONFH treatment approach based on M2 macrophages-derived exosomes(M2-Exos).We further show that M2-Exos are highly effective in the treatment of ONFH by modulating the phenotypes communication between neutrophil and endothelium including neutrophil extracellular traps formation and endothelial phenotype transition.Additionally,we identified that M2-Exos’therapeutic effect is attributed to the high content of miR-93-5p and constructed miR-93-5p overexpression model in vitro and in vivo based on lentivirus and adenoassociated virus respectively.Then we found miR-93-5p can not only reduce neutrophil extracellular traps formation but also improve angiogenic ability of endothelial cells.These results provided a new theoretical basis for the clinical application of ONFH therapeutic exosomes.
文摘In this paper we present how an serious security relevant event can be taken care of duing Olympic Games.Remote healthcare treatment will be given to injured persons during nuclear radiological biological attacks or in the case of physical disasters. The "Emergency-112" wireless telemedicine platform provides the hardware and software inrastructures in order to cope with the most extreme scenarios.A fully autonomous mobile system interconnects a group of injured persons with the expert medical institution.The unlimited capabilities of the system allows the Emergency-112 platform to be sed for rescue and surveillance operations regardless the terrain or the weather conditions.A dynamic hybrid system monitors the available spectrum and actively switches between different telecommunication access gateways.
