China’s“common prosperity”policy is a multidimensional pursuit of social and economic progress aimed at full realization by 2050.A CHIEVING common prosperity–prosperity that is universally shared–has been a longs...China’s“common prosperity”policy is a multidimensional pursuit of social and economic progress aimed at full realization by 2050.A CHIEVING common prosperity–prosperity that is universally shared–has been a longstanding aspiration of the Chinese government.展开更多
Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the i...Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the improvement of animal flesh quality.Panax ginseng leaf polysaccharides(PGLP)have a similar composition and lower cost compared with Panax ginseng root polysaccharides.However,its function and application effects in grass carp(Ctenopharyngodon idella)are unclear.Methods A total of 540 sub-adult grass carp(679±1.29 g),one of the important economic fish species,were used as experimental models and fed diets supplemented with 0,100,200,300,400,or 500 mg/kg PGLP for 60 d.After 60 d,grass carp were weighed,and their muscles were collected to explore the effects of PGLP on the growth and development of myofibers and energy metabolism-related parameters.Results Our study found that PGLP increased the growth performance and muscle nutritional composition as well as improved muscle hardness,springiness,cohesiveness,chewiness,and hyperplasia of myofibers of sub-adult grass carp.Besides,PGLP promoted muscle energy metabolism by increasing creatine,glycogen,pyruvate,and acetyl-CoA contents and creatine kinase(CK),pyruvate kinase(PK),phosphofructokinase(PFK),and hexokinase(HK)activities,while decreasing lactate dehydrogenase(LDH)activity and lactate content in fish muscle.Finally,our study found that PGLP enhanced mitochondrial function by increasing the protein expression of mitochondrial complexes I–V,biogenesis,and fusion and decreasing autophagy and fission in fish muscle.Conclusions PGLP improved growth performance and flesh quality of sub-adult grass carp,which may be related to enhancing hyperplasia of myofibers by promoting energy metabolism.We concluded that the recommended amount of PGLP in sub-adult grass carp feed to optimize growth performance is 100–200 mg/kg.This study provides a theoretical basis for the application of PGLP in fish feed and for the analysis of the mechanism of nutrition and feed regulating fish flesh quality,which is of great significance.展开更多
Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related c...Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related complications.We aimed to develop an artificial intelligence(AI)model for stent length selection during ERCP.Methods:Images of the patients who underwent ERCP and were diagnosed with CBD strictures were collected.Training involved identifying and delineating the duodenoscope,CBD and guidewire,calculating the pixel distance of the target guidewire and determining the required biliary stent length based on the diameter of the duodenoscope.The performance of the model,accuracy for length calculation and the assistance for endoscopists were validated using the testing set.Results:A total of 794 images from 431 patients were included and data augmentation was conducted.The mean intersection over union(mIoU)for duodenoscope,CBD and guidewire were 90.46%,84.79%and 84.64%,respectively.The accuracy in identifying the strictures was 97.58%(121/124).The accuracy for stent length calculation achieved 85.95%(104/121)with an error margin of±1 cm.The mean absolute error(MAE)and mean relative error(MRE)of the AI model was 0.81 cm and 0.13,respectively.The AI model could reduce approximately 202 mGycm^(2)of the radiation exposure for each patient.It significantly improved both MAE and MRE for less experienced endoscopists(P=0.01 and P=0.02,respectively).Conclusions:The AI model could accurately identify duodenoscope,CBD and guidewire,enabling accurate strictures identification and stent length selection.展开更多
Villager Pan Chunlin is witnessing a boom in his homestay business.More and more visitors are coming to his village,Yucun Village in Anji County,Huzhou City,Zhejiang Province.
Taking the rural low-income population of Zhejiang Province as its subject, this paper examines how to build a sustainable income-growth mechanism and identify feasible implementation paths within the context of the c...Taking the rural low-income population of Zhejiang Province as its subject, this paper examines how to build a sustainable income-growth mechanism and identify feasible implementation paths within the context of the common prosperity strategy. The research identifies key obstacles to income expansion, including an undiversified industrial structure, insufficient human capital, and a lack of robust social protection. These call for systemic solutions featuring institutional innovation, resource consolidation, and capability enhancement. Building on Zhejiang's experience as a common prosperity demonstration zone, the article constructs an integrated framework centered on four pillars: industrial empowerment, education upgrading, social security reinforcement, and digital coordination. It further offers concrete policy proposals involving the cultivation of localized industries, vocational skill training, enhanced safety nets, and the adoption of digital tools. The study thus offers both theoretical insights and practical paradigms for tackling the challenge of raising incomes in low-income rural areas.展开更多
The Chinese government’s common prosperity policy has inspired a series of rural development initiatives aimed at reducing income disparities between urban and rural areas.Zhejiang Province was designated as the firs...The Chinese government’s common prosperity policy has inspired a series of rural development initiatives aimed at reducing income disparities between urban and rural areas.Zhejiang Province was designated as the first demonstration zone in China’s pursuit of common prosperity.To explore the demonstration effect of rural development on narrowing the income gap between urban and rural areas,this study adopts the Theil index to measure urban-rural income disparity levels and explores spatial evolution patterns of the income disparity in 52 counties of Zhejiang Province,China from 2011 to 2020.The spatial Durbin model is also used to investigate the impact of rural development on the urban-rural income disparity.The results show that:1)Urban-rural income disparities have gradually narrowed.Moreover,income disparities across the province are spatially clustered from north to south.Narrowing the gap between mountainous and non-mountainous counties is imperative for resolving these spatial imbalances in the short term.2)Rural development reduces urban-rural income disparity levels and generates economic and technological spillover effects,as evidenced by the phenomenon where development in one county leads to reduced income disparities in neighboring counties.3)The technological innovation enhances the co-development of rural industries through technological diffusion effects,further strengthening rural development.Finally,enhancing the ecological environment synergizes with rural development to reduce urban-rural income disparities.The research results provide policy implications for narrowing the urban-rural income disparity and promoting common prosperity.展开更多
During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per gene...During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.展开更多
This study was carried out to study the effect of adding different levels of date palm seed （Phoentix dactyliJProus） powder in the diet of common carp （Cyprinus carpio） at fish laboratory of Animal Production Depa...This study was carried out to study the effect of adding different levels of date palm seed （Phoentix dactyliJProus） powder in the diet of common carp （Cyprinus carpio） at fish laboratory of Animal Production Department, College of Agricultural Sciences, Sulaimani University, Sulaimani, Iraq. The actual experimental feeding trials were conducted for 70 d and the adaptation period for 21 d. A total of 81 common carp fingerlings weighted 62 ± 2 g were divided into three experimental groups. The control treatment T1 was with 0 g date palm seed, T2 with 2.5 g date palm seed per kg diet and T3 with 5 g date palm seed per kg diet. Each treatment was in three replicates in which three common carp fingerlings were stocked in each aquarium which fed the experimental diets twice daily. The results indicated that weight gain （WG） and specific growth rate （SGR） in the third treatment （T3） group were significantly higher than those of fish fed control diets. T2 consumed more diet than other treatments with 306.940 g with higher feed conversion ratio （FCR）; T3 was higher significantly in food efficiency. No significant （P 〈 0.05） differences obtained from consumed protein while the protein efficiency ratio was significantly higher in T3 with 319.323. Control was higher significantly in flesh weight without viscera with the third treatment; in flesh weight without head and viscera each the control and the second treatment was significantly higher than the third treatment. As a conclusion, the date palm seeds powder have a positive effect on growth performance of common carp.展开更多
Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass pr...Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus.展开更多
Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before Decem...Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before December 31, 2004. A complete search of 2-6 nucleotide microsatellites resulted in the identification of 513 SSR-containing ESTs, accounting for 4.8% of the total. Cluster analysis indicated that 73 sequences of SSR-containing ESTs fell into 27 groups and the remaining 440 ESTs were independent. A total of 467 unique SSR-containing ESTs were identified. These EST-SSRs contained a vari- ety of simple sequence types, and di- and tri-nucleotide repeats were the most abundant, accounting for 42.1% and 27.9% of the whole, respectively. Of the dinucleotide repeats, CA/TG was the most abundant, followed by GA/TC. BLASTx search showed that 38.1% of the SSR loci could be associated with genes or proteins of known or unknown function. BLASTx searches of SSR-containing ESTs also showed high frequencies （98/179） of hits on zebrafish sequences.展开更多
Genome evolution arises from two main ways of duplication and reduction. Fish specific genome duplication （FSGD） may have occurred before the radiation of the teleosts. Common carp （Cyprinus carpio L.） has been co...Genome evolution arises from two main ways of duplication and reduction. Fish specific genome duplication （FSGD） may have occurred before the radiation of the teleosts. Common carp （Cyprinus carpio L.） has been considered to be a tetraploid species, because of its chromosome numbers （2n=100） and its high DNA content. Using 69 microsatellite primer pairs, the variations were studied to better understand the genome evolution （genome duplication and diploidization） of common carp from a gynogenetic family. About 48% of primer pairs were estimated to amplify duplicates based on the number of PCR amplification per individual. Segregation patterns in the family suggested a partially duplicated genome structure and disomic inheritance. This indicates that the common carp is tetraploid and polyploidy occurred by allotetraploidy. Two primer pairs （HLJ021 and HLJ332） were estimated to amplify reduction based on the number of PCR amplification per individual. One allele in HLJ002 locus and HLJ332 locus was clearly lost in the gynogenetic family and the same as in six wild populations. Segregation patterns in the family suggested a partially diplodization genome structure. A hypothesis transition （dynamic） and equilibrium （static） were proposed to explain the common carp genome evolution between genome duplication and diploidization.展开更多
Thirty microsatellite loci were used for analyzing six wild populations of common carp(Cyprinus carpio L.).Observed(Ho)and expected(He)heterozygosity values,polymorphic information content(PIC),and number of effective...Thirty microsatellite loci were used for analyzing six wild populations of common carp(Cyprinus carpio L.).Observed(Ho)and expected(He)heterozygosity values,polymorphic information content(PIC),and number of effective alleles(Ae)were all detected.Genetic similarity index and genetic distance were computed based on the allele frequency.The Hardy-Weinberg Equilibrium was checked according to the test ofχ^2.Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm.A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package(version 3.63).There were totally 8,136 fragments ranging from 125 bp to 414 bp in length.Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations.The average number of alleles in each locus was seven.The result showed that 1)the level of genetic variability was moderate in the six populations.Polymorphic information contents of the six wild common carp populations were 0.44,0.52,0.53,0.57,0.63,and 0.64 respectively.Effective alleles were from 1.04 to 4.72,the average numbers in each population were 2.19,2.60,2.42,2.43,2.45,and 2.33.The average expected heterozygosity values were 0.50,0.59,0.56,0.56,0.57,and 0.54 respectively;2)the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688,and it came from the populations of BR and HN.There was a correlation between the clustering result and the geographical distribution.展开更多
This experiment was an attempt to use the soybean residue derived from the production of 'soy milk'. The residue contains about 18% protein, 70% caf bohydrates and 7.5% lipid as fish feed for reafing common ca...This experiment was an attempt to use the soybean residue derived from the production of 'soy milk'. The residue contains about 18% protein, 70% caf bohydrates and 7.5% lipid as fish feed for reafing common carp, Cyrinus carpto. There were 4 types of diets: (1)soybean residue, (2 ) soybean residue digested with Papain, (3) soybean residue (64%)mixed with beef liver (34%) and (4) same mixture as (3) but digested with Papain. The results indicate that the percentage increase in weight and length of fish feeding with beef liver supplemented diets was higher than those feeding with soybean residue alone. This was possibly due to the fact that beef liver was able to supplement the nutrient deficiency in soybean. The two types of feeds (2 and 4) digested with Papain also yielded significantly better fish growth in terms of weight and length gains, than their counterparts without digestion. Furthermore, the water turbidity of the tanks added with digested feeds was significantly less, as papain was able to hyrolyse the protein substrates suspended in the water, and thus lowered the turbidity展开更多
A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of e...A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of enzymes in common carp,Cyprinus carpio(mean body weight,36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate.Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%,13%,19.5%,or 26%and protein levels of 30.5%,28.2%,26.4%,and 24.2%.The results showed no differences in growth performance of fish fed the diets with different protein and corn starch levels,but body composition and glucose metabolic enzyme activity of carp were significantly affected by the different diets(P<0.05).Weight gain,specific growth rate,and the feed conversion ratio were not different in fish fed the different dietary treatments.Protein efficiency ratio increased significantly as corn starch level increased(P<0.05).Whole-body crude lipid composition increased with increasing dietary corn starch level(P<0.05).Glucokinase(GK),hexokinase,and pyruvate kinase(PK) activities increased significantly with increasing dietary corn starch level(P<0.05),whereas glucose-6-phosphate(G6Pase) activity decreased with increasing dietary corn starch level(P<0.05).GK gene expression was significantly higher in fish fed the high-corn starch diet than those fed the low-corn starch diet(P<0.05).G6 pase gene expression tended to decrease with increasing starch level(P>0.05).In summary,the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.展开更多
The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies o...The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies of thepMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites. By using a method of plasmid rescue, four hundred copies of transgenes fromtwo individuals of F4 transgenic fish, A and B, were recovered and clarified into 6 classes. All classes of recoveredtransgenes contained either complete or partial pMThGH sequences. The class I, which comprised 83% and 84.5%respectively of the recovered transgene copies from fish A and B, had maintained the original configuration, indicatingthat most transgenes were faithfully inherited during the four generations of reproduction. The other five classes weredifferent from the original configuration in both molecular weight and restriction map, indicating that a few transgeneshad undergone mutation, rearrangement or deletion during integration and germline transmission. In the five types ofaberrant transgenes, three flanking sequences of the host genome were analyzed. These sequences were common carpβ-actin gene, common carp DNA sequences homologous to mouse phosphoglycerate kinase-1 and human epidermalkeratin 14, respectively.展开更多
Phytoremediation can be used as a sustainable technology for mine spoil remediation to remove heavy metals. This study investigated the concentration of 7 heavy metal contamination in soil and plant samples at an aban...Phytoremediation can be used as a sustainable technology for mine spoil remediation to remove heavy metals. This study investigated the concentration of 7 heavy metal contamination in soil and plant samples at an abandoned mine site. We found that, after vegetation remediation at the abandoned mine site, the reduction rates for 7 heavy metals were in the range of 4.2%-86%, where reduction rates over 50% were achieved for four heavy metals （Zn, Mn, Cd, Ni）. Transfer coefficients of the panicled goldenrain tree （Koelreuteria paniculata Laxm） and the common elaeocarpus （Elaeocarpus decipens） for Zn, Mn, Ni, and Co were more than 1. Enrichment coefficients of both trees for Mn were higher than 1. Our results suggest that the panicled goldenrain tree and the common elaeocarpus tree may act as accumulators in remediation. Moreover, the woody vegetation remediation in abandoned mining areas play an important role in improving scenery besides removing heavy metal from contaminated soil.展开更多
Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium c...Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, △5-3β-HSD and 17β-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 μg/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.展开更多
Feeding rhythm of common carp was investigated from 4℃ to 34℃. The results indicated that there was a diel feeding rhythm for both adult （630-850 g） and youth （61-91 g） at all tested temperatures. There were two...Feeding rhythm of common carp was investigated from 4℃ to 34℃. The results indicated that there was a diel feeding rhythm for both adult （630-850 g） and youth （61-91 g） at all tested temperatures. There were two main activity peaks at 8：00-11：00 a.m. and 19：00-23：00 p.m., during which feeding quantities were 10.68%-32.53% and 16.25%-33.41% of the daily intake, respectively. When water temperature dropped to below 10℃, the feeding peak concentrated at 8：00-11：00 a.m. and 19：00 p.m. to 4：00 a.m. At 6℃, though both adult and youth would still feed, the feeding quantities were only 0.01% and 0.35% of body mass. Daily feeding rate of adult and youth reached 1.21% and 2.63% at 14℃, respectively. Both adult and youth carps reached the maximum daily feeding rate at 28℃, being 2.84% and 12.06% of body mass, respectively. The daily feeding rate of adult and youth reduced suddenly after at 34℃, and the daily feeding rate was only 0.74% and 9.45% of body mass, respectively. There was significant difference in daily feeding rate at different water temperatures （P〈0.05）.展开更多
The biological hydrogen generating from fermentation of low-cost lignocellulosic feedstocks by hydrogen-producing bacteria has attracted many attentions in recent years. In the present investigation, ten hydrogen-prod...The biological hydrogen generating from fermentation of low-cost lignocellulosic feedstocks by hydrogen-producing bacteria has attracted many attentions in recent years. In the present investigation, ten hydrogen-producing bacteria were newly isolated from the intestine of wild common carp (</span><span style="font-family:Verdana;"><i>Cyprinus carpio</i></span><span style="font-family:Verdana;"> L.), and identified belonging to the genera of </span><i><span style="font-family:Verdana;">Enterobacter</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Klebsiella</span></i><span style="font-family:Verdana;"> based on analysis of the 16S rDNA gene sequence and examination of the physiological and biochemical characteristics. All the isolates inherently owned the ability to metabolize xylose especially the cotton stalk hydrolysate for hydrogen production with hydrogen yield (HY) higher than 100 mL</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;">·</span></span><span></span><span></span><span style="font-family:""><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">. In particular, two isolates, WL1306 and WL1305 obtained higher HY, hydrogen production rate (HPR), and hydrogen production potential (HPP) using cotton stalk hydrolysate as sugar substrate than the mixed sugar of glucose & xylose, which obtained the HY of 249.5 ± 29.0, 397.0 ± 36.7 mL</span></span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">, HPR of 10.4 ± 1.2, 16.5 ± 1.5 mL</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">h</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">, HPP of 19.5 ± 2.3, 31.0 ± 2.8 mL</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">g</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><sub><span style="font-family:Verdana;">sugar</span></sub><span style="font-family:Verdana;">, separately. The generation of soluble metabolites, such as the lactate, formate, acetate, succinate and ethanol reflected the mixed acid fermentation properties of the hydrogen production pathway.展开更多
Physicochemical and sensory characteristics of restructured meat of common carp （Cyprinus carpio） fortified with 0-8 g/100 g of chia seed flour （CSF） was evaluated. It had a higher nutritional value （higher fibre...Physicochemical and sensory characteristics of restructured meat of common carp （Cyprinus carpio） fortified with 0-8 g/100 g of chia seed flour （CSF） was evaluated. It had a higher nutritional value （higher fibre content and protein retention] （p 〈 0.05） and better cooking characteristics （higher cooking yield and moisture retention） （p 〈 0.05） than the control. The colour （a＊, b＊） increased; lightness and whiteness index decrease （p 〈 0.05）. Hardness increase （p 〈 0.05） occurred because of CSF addition. Differential scanning calorimetry showed that fibre fortification did not interfere with the thermal transitions of the restructured meat. No significant differences were detected with the preference test scores of 4% or 8% CSF compared with the control. Restructured （4%-8% CSF） had a higher content of fibre and fat, which could be linoleic and linolenic acid, and an increase in the content of protein compared with those of commercial products, among had 1.62 and 2.25 mg AGE/g. Therefore, the restructured properties of common carp were governed by CSF addition.展开更多
文摘China’s“common prosperity”policy is a multidimensional pursuit of social and economic progress aimed at full realization by 2050.A CHIEVING common prosperity–prosperity that is universally shared–has been a longstanding aspiration of the Chinese government.
基金supported by National Natural Science Foundation of China(U23A20250)the earmarked fund for CARS(CARS-45)+2 种基金the National Key R&D Program of China(2023YFD2400600)Sichuan Provincial Science and Technology Innovation Talent Project(2023JDRC0043)Sichuan Innovation Team of National Modern Agricultural Industry Technology System(SCCXTD-2024-15)。
文摘Background As living standards improve,consumers are placing greater emphasis on the enhancement of fish flesh quality,making its improvement increasingly critical.Plant-derived polysaccharides positively affect the improvement of animal flesh quality.Panax ginseng leaf polysaccharides(PGLP)have a similar composition and lower cost compared with Panax ginseng root polysaccharides.However,its function and application effects in grass carp(Ctenopharyngodon idella)are unclear.Methods A total of 540 sub-adult grass carp(679±1.29 g),one of the important economic fish species,were used as experimental models and fed diets supplemented with 0,100,200,300,400,or 500 mg/kg PGLP for 60 d.After 60 d,grass carp were weighed,and their muscles were collected to explore the effects of PGLP on the growth and development of myofibers and energy metabolism-related parameters.Results Our study found that PGLP increased the growth performance and muscle nutritional composition as well as improved muscle hardness,springiness,cohesiveness,chewiness,and hyperplasia of myofibers of sub-adult grass carp.Besides,PGLP promoted muscle energy metabolism by increasing creatine,glycogen,pyruvate,and acetyl-CoA contents and creatine kinase(CK),pyruvate kinase(PK),phosphofructokinase(PFK),and hexokinase(HK)activities,while decreasing lactate dehydrogenase(LDH)activity and lactate content in fish muscle.Finally,our study found that PGLP enhanced mitochondrial function by increasing the protein expression of mitochondrial complexes I–V,biogenesis,and fusion and decreasing autophagy and fission in fish muscle.Conclusions PGLP improved growth performance and flesh quality of sub-adult grass carp,which may be related to enhancing hyperplasia of myofibers by promoting energy metabolism.We concluded that the recommended amount of PGLP in sub-adult grass carp feed to optimize growth performance is 100–200 mg/kg.This study provides a theoretical basis for the application of PGLP in fish feed and for the analysis of the mechanism of nutrition and feed regulating fish flesh quality,which is of great significance.
基金supported by grants from the Taishan Scholars Program of Shandong Province(tsqn202312333)the National Natural Science Foundation of China(82470695).
文摘Background:Biliary stent placement during endoscopic retrograde cholangiopancreatography(ERCP)is important for drainage in common bile duct(CBD)strictures,while the stent length is associated with many stent-related complications.We aimed to develop an artificial intelligence(AI)model for stent length selection during ERCP.Methods:Images of the patients who underwent ERCP and were diagnosed with CBD strictures were collected.Training involved identifying and delineating the duodenoscope,CBD and guidewire,calculating the pixel distance of the target guidewire and determining the required biliary stent length based on the diameter of the duodenoscope.The performance of the model,accuracy for length calculation and the assistance for endoscopists were validated using the testing set.Results:A total of 794 images from 431 patients were included and data augmentation was conducted.The mean intersection over union(mIoU)for duodenoscope,CBD and guidewire were 90.46%,84.79%and 84.64%,respectively.The accuracy in identifying the strictures was 97.58%(121/124).The accuracy for stent length calculation achieved 85.95%(104/121)with an error margin of±1 cm.The mean absolute error(MAE)and mean relative error(MRE)of the AI model was 0.81 cm and 0.13,respectively.The AI model could reduce approximately 202 mGycm^(2)of the radiation exposure for each patient.It significantly improved both MAE and MRE for less experienced endoscopists(P=0.01 and P=0.02,respectively).Conclusions:The AI model could accurately identify duodenoscope,CBD and guidewire,enabling accurate strictures identification and stent length selection.
文摘Villager Pan Chunlin is witnessing a boom in his homestay business.More and more visitors are coming to his village,Yucun Village in Anji County,Huzhou City,Zhejiang Province.
文摘Taking the rural low-income population of Zhejiang Province as its subject, this paper examines how to build a sustainable income-growth mechanism and identify feasible implementation paths within the context of the common prosperity strategy. The research identifies key obstacles to income expansion, including an undiversified industrial structure, insufficient human capital, and a lack of robust social protection. These call for systemic solutions featuring institutional innovation, resource consolidation, and capability enhancement. Building on Zhejiang's experience as a common prosperity demonstration zone, the article constructs an integrated framework centered on four pillars: industrial empowerment, education upgrading, social security reinforcement, and digital coordination. It further offers concrete policy proposals involving the cultivation of localized industries, vocational skill training, enhanced safety nets, and the adoption of digital tools. The study thus offers both theoretical insights and practical paradigms for tackling the challenge of raising incomes in low-income rural areas.
基金Under the auspices of Project of Humanities and Social Sciences Research of the Ministry of Education(No.23YJC790127)Major projects of the National Social Science Fund(No.18ZDA045)。
文摘The Chinese government’s common prosperity policy has inspired a series of rural development initiatives aimed at reducing income disparities between urban and rural areas.Zhejiang Province was designated as the first demonstration zone in China’s pursuit of common prosperity.To explore the demonstration effect of rural development on narrowing the income gap between urban and rural areas,this study adopts the Theil index to measure urban-rural income disparity levels and explores spatial evolution patterns of the income disparity in 52 counties of Zhejiang Province,China from 2011 to 2020.The spatial Durbin model is also used to investigate the impact of rural development on the urban-rural income disparity.The results show that:1)Urban-rural income disparities have gradually narrowed.Moreover,income disparities across the province are spatially clustered from north to south.Narrowing the gap between mountainous and non-mountainous counties is imperative for resolving these spatial imbalances in the short term.2)Rural development reduces urban-rural income disparity levels and generates economic and technological spillover effects,as evidenced by the phenomenon where development in one county leads to reduced income disparities in neighboring counties.3)The technological innovation enhances the co-development of rural industries through technological diffusion effects,further strengthening rural development.Finally,enhancing the ecological environment synergizes with rural development to reduce urban-rural income disparities.The research results provide policy implications for narrowing the urban-rural income disparity and promoting common prosperity.
文摘During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.
文摘This study was carried out to study the effect of adding different levels of date palm seed （Phoentix dactyliJProus） powder in the diet of common carp （Cyprinus carpio） at fish laboratory of Animal Production Department, College of Agricultural Sciences, Sulaimani University, Sulaimani, Iraq. The actual experimental feeding trials were conducted for 70 d and the adaptation period for 21 d. A total of 81 common carp fingerlings weighted 62 ± 2 g were divided into three experimental groups. The control treatment T1 was with 0 g date palm seed, T2 with 2.5 g date palm seed per kg diet and T3 with 5 g date palm seed per kg diet. Each treatment was in three replicates in which three common carp fingerlings were stocked in each aquarium which fed the experimental diets twice daily. The results indicated that weight gain （WG） and specific growth rate （SGR） in the third treatment （T3） group were significantly higher than those of fish fed control diets. T2 consumed more diet than other treatments with 306.940 g with higher feed conversion ratio （FCR）; T3 was higher significantly in food efficiency. No significant （P 〈 0.05） differences obtained from consumed protein while the protein efficiency ratio was significantly higher in T3 with 319.323. Control was higher significantly in flesh weight without viscera with the third treatment; in flesh weight without head and viscera each the control and the second treatment was significantly higher than the third treatment. As a conclusion, the date palm seeds powder have a positive effect on growth performance of common carp.
基金the National Key Research and Development Program,China(2018YFD0900205)to Zhan Yinthe National Natural Science Foundation,China(31972779 and 31530077)to Gang Zhai and Zhan Yin+2 种基金the Pilot Program A Project from the Chinese Academy of Sciences(XDA24010206)to Zhan Yinthe Youth Innovation Promotion Association of CAS(20200336)to Gang Zhaithe State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)to Zhan Yin.
文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus.
文摘Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before December 31, 2004. A complete search of 2-6 nucleotide microsatellites resulted in the identification of 513 SSR-containing ESTs, accounting for 4.8% of the total. Cluster analysis indicated that 73 sequences of SSR-containing ESTs fell into 27 groups and the remaining 440 ESTs were independent. A total of 467 unique SSR-containing ESTs were identified. These EST-SSRs contained a vari- ety of simple sequence types, and di- and tri-nucleotide repeats were the most abundant, accounting for 42.1% and 27.9% of the whole, respectively. Of the dinucleotide repeats, CA/TG was the most abundant, followed by GA/TC. BLASTx search showed that 38.1% of the SSR loci could be associated with genes or proteins of known or unknown function. BLASTx searches of SSR-containing ESTs also showed high frequencies （98/179） of hits on zebrafish sequences.
文摘Genome evolution arises from two main ways of duplication and reduction. Fish specific genome duplication （FSGD） may have occurred before the radiation of the teleosts. Common carp （Cyprinus carpio L.） has been considered to be a tetraploid species, because of its chromosome numbers （2n=100） and its high DNA content. Using 69 microsatellite primer pairs, the variations were studied to better understand the genome evolution （genome duplication and diploidization） of common carp from a gynogenetic family. About 48% of primer pairs were estimated to amplify duplicates based on the number of PCR amplification per individual. Segregation patterns in the family suggested a partially duplicated genome structure and disomic inheritance. This indicates that the common carp is tetraploid and polyploidy occurred by allotetraploidy. Two primer pairs （HLJ021 and HLJ332） were estimated to amplify reduction based on the number of PCR amplification per individual. One allele in HLJ002 locus and HLJ332 locus was clearly lost in the gynogenetic family and the same as in six wild populations. Segregation patterns in the family suggested a partially diplodization genome structure. A hypothesis transition （dynamic） and equilibrium （static） were proposed to explain the common carp genome evolution between genome duplication and diploidization.
基金This work is supported by the Key Project of Chinese National Program for Fundamental Research and Development(No.2004CB-117405)Chinese commonweal Program(No.2005DIB4J024).
文摘Thirty microsatellite loci were used for analyzing six wild populations of common carp(Cyprinus carpio L.).Observed(Ho)and expected(He)heterozygosity values,polymorphic information content(PIC),and number of effective alleles(Ae)were all detected.Genetic similarity index and genetic distance were computed based on the allele frequency.The Hardy-Weinberg Equilibrium was checked according to the test ofχ^2.Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm.A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package(version 3.63).There were totally 8,136 fragments ranging from 125 bp to 414 bp in length.Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations.The average number of alleles in each locus was seven.The result showed that 1)the level of genetic variability was moderate in the six populations.Polymorphic information contents of the six wild common carp populations were 0.44,0.52,0.53,0.57,0.63,and 0.64 respectively.Effective alleles were from 1.04 to 4.72,the average numbers in each population were 2.19,2.60,2.42,2.43,2.45,and 2.33.The average expected heterozygosity values were 0.50,0.59,0.56,0.56,0.57,and 0.54 respectively;2)the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688,and it came from the populations of BR and HN.There was a correlation between the clustering result and the geographical distribution.
文摘This experiment was an attempt to use the soybean residue derived from the production of 'soy milk'. The residue contains about 18% protein, 70% caf bohydrates and 7.5% lipid as fish feed for reafing common carp, Cyrinus carpto. There were 4 types of diets: (1)soybean residue, (2 ) soybean residue digested with Papain, (3) soybean residue (64%)mixed with beef liver (34%) and (4) same mixture as (3) but digested with Papain. The results indicate that the percentage increase in weight and length of fish feeding with beef liver supplemented diets was higher than those feeding with soybean residue alone. This was possibly due to the fact that beef liver was able to supplement the nutrient deficiency in soybean. The two types of feeds (2 and 4) digested with Papain also yielded significantly better fish growth in terms of weight and length gains, than their counterparts without digestion. Furthermore, the water turbidity of the tanks added with digested feeds was significantly less, as papain was able to hyrolyse the protein substrates suspended in the water, and thus lowered the turbidity
基金Supported by the Key Technology R&D Program of Tianjin(No.13ZCZDNC00900)the Tianjin Research Program of Application Foundation and Advanced Technology(No.14JCQNJC15100)+2 种基金the Tianjin Innovative Research Team(No.TD12-5018)the Tianjin Key Laboratory of Animal and Plant Resistancethe National Natural Science Foundation of China(No.31402313)
文摘A 9-week feeding trial in floating freshwater cages(1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth,body composition,and gene expression of enzymes in common carp,Cyprinus carpio(mean body weight,36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate.Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%,13%,19.5%,or 26%and protein levels of 30.5%,28.2%,26.4%,and 24.2%.The results showed no differences in growth performance of fish fed the diets with different protein and corn starch levels,but body composition and glucose metabolic enzyme activity of carp were significantly affected by the different diets(P<0.05).Weight gain,specific growth rate,and the feed conversion ratio were not different in fish fed the different dietary treatments.Protein efficiency ratio increased significantly as corn starch level increased(P<0.05).Whole-body crude lipid composition increased with increasing dietary corn starch level(P<0.05).Glucokinase(GK),hexokinase,and pyruvate kinase(PK) activities increased significantly with increasing dietary corn starch level(P<0.05),whereas glucose-6-phosphate(G6Pase) activity decreased with increasing dietary corn starch level(P<0.05).GK gene expression was significantly higher in fish fed the high-corn starch diet than those fed the low-corn starch diet(P<0.05).G6 pase gene expression tended to decrease with increasing starch level(P>0.05).In summary,the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.
基金supported bythe Major State Basic Research Development Program ofChina (No. 2004CB117406 and G2000016109)the National Natural Science Foundation of China (No.90208024 and 39823003).
文摘The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies of thepMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites. By using a method of plasmid rescue, four hundred copies of transgenes fromtwo individuals of F4 transgenic fish, A and B, were recovered and clarified into 6 classes. All classes of recoveredtransgenes contained either complete or partial pMThGH sequences. The class I, which comprised 83% and 84.5%respectively of the recovered transgene copies from fish A and B, had maintained the original configuration, indicatingthat most transgenes were faithfully inherited during the four generations of reproduction. The other five classes weredifferent from the original configuration in both molecular weight and restriction map, indicating that a few transgeneshad undergone mutation, rearrangement or deletion during integration and germline transmission. In the five types ofaberrant transgenes, three flanking sequences of the host genome were analyzed. These sequences were common carpβ-actin gene, common carp DNA sequences homologous to mouse phosphoglycerate kinase-1 and human epidermalkeratin 14, respectively.
基金As a key project under the State Forestry Administration of China (2006-11, 2006-17, 2005-08)this project was funded by the National Natural Science Foundation of China (No. 30571487, 30771700)+1 种基金the Furong Scholar Program, the Urban Forest Ecological Key Laboratory of Hunan Province (No. 06FJ3083)the Platform Construction Project under the Ministry of Science and Technology of China
文摘Phytoremediation can be used as a sustainable technology for mine spoil remediation to remove heavy metals. This study investigated the concentration of 7 heavy metal contamination in soil and plant samples at an abandoned mine site. We found that, after vegetation remediation at the abandoned mine site, the reduction rates for 7 heavy metals were in the range of 4.2%-86%, where reduction rates over 50% were achieved for four heavy metals （Zn, Mn, Cd, Ni）. Transfer coefficients of the panicled goldenrain tree （Koelreuteria paniculata Laxm） and the common elaeocarpus （Elaeocarpus decipens） for Zn, Mn, Ni, and Co were more than 1. Enrichment coefficients of both trees for Mn were higher than 1. Our results suggest that the panicled goldenrain tree and the common elaeocarpus tree may act as accumulators in remediation. Moreover, the woody vegetation remediation in abandoned mining areas play an important role in improving scenery besides removing heavy metal from contaminated soil.
文摘Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, △5-3β-HSD and 17β-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 μg/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.
文摘Feeding rhythm of common carp was investigated from 4℃ to 34℃. The results indicated that there was a diel feeding rhythm for both adult （630-850 g） and youth （61-91 g） at all tested temperatures. There were two main activity peaks at 8：00-11：00 a.m. and 19：00-23：00 p.m., during which feeding quantities were 10.68%-32.53% and 16.25%-33.41% of the daily intake, respectively. When water temperature dropped to below 10℃, the feeding peak concentrated at 8：00-11：00 a.m. and 19：00 p.m. to 4：00 a.m. At 6℃, though both adult and youth would still feed, the feeding quantities were only 0.01% and 0.35% of body mass. Daily feeding rate of adult and youth reached 1.21% and 2.63% at 14℃, respectively. Both adult and youth carps reached the maximum daily feeding rate at 28℃, being 2.84% and 12.06% of body mass, respectively. The daily feeding rate of adult and youth reduced suddenly after at 34℃, and the daily feeding rate was only 0.74% and 9.45% of body mass, respectively. There was significant difference in daily feeding rate at different water temperatures （P〈0.05）.
文摘The biological hydrogen generating from fermentation of low-cost lignocellulosic feedstocks by hydrogen-producing bacteria has attracted many attentions in recent years. In the present investigation, ten hydrogen-producing bacteria were newly isolated from the intestine of wild common carp (</span><span style="font-family:Verdana;"><i>Cyprinus carpio</i></span><span style="font-family:Verdana;"> L.), and identified belonging to the genera of </span><i><span style="font-family:Verdana;">Enterobacter</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Klebsiella</span></i><span style="font-family:Verdana;"> based on analysis of the 16S rDNA gene sequence and examination of the physiological and biochemical characteristics. All the isolates inherently owned the ability to metabolize xylose especially the cotton stalk hydrolysate for hydrogen production with hydrogen yield (HY) higher than 100 mL</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;">·</span></span><span></span><span></span><span style="font-family:""><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">. In particular, two isolates, WL1306 and WL1305 obtained higher HY, hydrogen production rate (HPR), and hydrogen production potential (HPP) using cotton stalk hydrolysate as sugar substrate than the mixed sugar of glucose & xylose, which obtained the HY of 249.5 ± 29.0, 397.0 ± 36.7 mL</span></span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">, HPR of 10.4 ± 1.2, 16.5 ± 1.5 mL</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">h</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">, HPP of 19.5 ± 2.3, 31.0 ± 2.8 mL</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">L</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">g</span><sup><span style="font-family:Verdana;">-</span></sup><sup><span style="font-family:Verdana;">1</span></sup><sub><span style="font-family:Verdana;">sugar</span></sub><span style="font-family:Verdana;">, separately. The generation of soluble metabolites, such as the lactate, formate, acetate, succinate and ethanol reflected the mixed acid fermentation properties of the hydrogen production pathway.
文摘Physicochemical and sensory characteristics of restructured meat of common carp （Cyprinus carpio） fortified with 0-8 g/100 g of chia seed flour （CSF） was evaluated. It had a higher nutritional value （higher fibre content and protein retention] （p 〈 0.05） and better cooking characteristics （higher cooking yield and moisture retention） （p 〈 0.05） than the control. The colour （a＊, b＊） increased; lightness and whiteness index decrease （p 〈 0.05）. Hardness increase （p 〈 0.05） occurred because of CSF addition. Differential scanning calorimetry showed that fibre fortification did not interfere with the thermal transitions of the restructured meat. No significant differences were detected with the preference test scores of 4% or 8% CSF compared with the control. Restructured （4%-8% CSF） had a higher content of fibre and fat, which could be linoleic and linolenic acid, and an increase in the content of protein compared with those of commercial products, among had 1.62 and 2.25 mg AGE/g. Therefore, the restructured properties of common carp were governed by CSF addition.
