Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of C...Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD1-12). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy.?Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC50 values 3.79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC50 value 30.52 μg/ml), CD11 (IC50 value 39.27 μg/ml) and CD12 (IC50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.展开更多
Polluted populations of Euphorbia heterophylla, Chromolaena odorata, Commelina diffusa and Kyllinga pumila were collected fresh from 0.1 m to 0.25 m radii of the exhausted-pipe of power generators used for domestic pu...Polluted populations of Euphorbia heterophylla, Chromolaena odorata, Commelina diffusa and Kyllinga pumila were collected fresh from 0.1 m to 0.25 m radii of the exhausted-pipe of power generators used for domestic purposes, whereas, their non-polluted counterparts were collected from distances of 100-110 m away. The length and width of 10 leaves, each of both polluted and non-polluted populations, were measured. Their average leaf areas （LA） were 2.60 ± 0.1 cm2, 14.16 ±0.4 cm2, 5.19 ± 0.8 cm2 and 1.80 ±0.8 cm2 for the polluted populations of each of the species, respectively. Whereas, the LA of their non-polluted counterparts were 4.18±0.7 cm2, 34.39 ±0.2 cm2, 7.51± 0.1 cm2 and 10.76 ± 0.3 cm2, respectively. Damages such as plugged stomatal pores, epidermal cell aberrations and erosion, ruptured stomatal ledges, occasional leaf perforations, irregularly fused cell boundaries and glued leaf surfaces were noticeable in the leaves of the polluted populations. Such were not observed in the non-polluted populations. It was suggested that foliar morphology of these group of plants could serve as phytometer to gauge the threats of power generators used in homes to terrestrial ecosystem.展开更多
文摘Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD1-12). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy.?Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC50 values 3.79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC50 value 30.52 μg/ml), CD11 (IC50 value 39.27 μg/ml) and CD12 (IC50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.
文摘Polluted populations of Euphorbia heterophylla, Chromolaena odorata, Commelina diffusa and Kyllinga pumila were collected fresh from 0.1 m to 0.25 m radii of the exhausted-pipe of power generators used for domestic purposes, whereas, their non-polluted counterparts were collected from distances of 100-110 m away. The length and width of 10 leaves, each of both polluted and non-polluted populations, were measured. Their average leaf areas （LA） were 2.60 ± 0.1 cm2, 14.16 ±0.4 cm2, 5.19 ± 0.8 cm2 and 1.80 ±0.8 cm2 for the polluted populations of each of the species, respectively. Whereas, the LA of their non-polluted counterparts were 4.18±0.7 cm2, 34.39 ±0.2 cm2, 7.51± 0.1 cm2 and 10.76 ± 0.3 cm2, respectively. Damages such as plugged stomatal pores, epidermal cell aberrations and erosion, ruptured stomatal ledges, occasional leaf perforations, irregularly fused cell boundaries and glued leaf surfaces were noticeable in the leaves of the polluted populations. Such were not observed in the non-polluted populations. It was suggested that foliar morphology of these group of plants could serve as phytometer to gauge the threats of power generators used in homes to terrestrial ecosystem.
