Backgroud:Coltiviruses are spherical,non-enveloped viruses with 12 double-stranded RNA segments,belonging to the family Spinareoviridae,and predominantly transmitted by ticks.This study isolated and characterized a no...Backgroud:Coltiviruses are spherical,non-enveloped viruses with 12 double-stranded RNA segments,belonging to the family Spinareoviridae,and predominantly transmitted by ticks.This study isolated and characterized a novel coltivirus,designated Woodland tick reovirus(WLTRV),from Haemaphysalis concinna ticks collected in Helong City,Jilin Province,in Northeastern China.Methods SW-13 and Vero cells were used to isolate WLTRV through three blind passages,while seven mammalian cell lines assessed viral growth.Viral morphology was observed by electron microscopy.Next-generation sequencing,5ʹand 3ʹrapid amplification of cDNA ends were used to determine WLTRV whole genome sequences,and phylogenetic methods were used to characterize WLTRV.A real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect WLTRV RNA in ticks.Results:WLTRV grew and exerted cytopathic effects in human(SW-13 and 293T)and mouse(BHK-21 and N2A)cell lines,revealing its potential to infect mammals.Phylogenetic analysis based on RNA-dependent RNA polymerase sequences classified WLTRV within the genus Coltivirus,with a close evolutionary relationship to Tarumizu tick virus.The nucleotide and amino acid sequence homologies between WLTRV and Tarumizu tick virus across the 12 segments analyzed ranged from approximately 44.79%to 69.09%and 33.73%to 75.60%,respectively.WLTRV shared conserved the 5ʹ-terminal(GACAA/UU/A)and 3ʹ-terminal(UGCAGUC)consensus sequences of the genus Coltivirus genomes.Electron microscopy revealed WLTRV as spherical(diameter∼80 nm),non-enveloped,and morphologically consistent with coltiviruses.Among the 4,717 ticks collected from six towns in the Yanbian Korean Autonomous Prefecture,WLTRV RNA was only detected in H.concinna(0.95%virus-carrying rate)but not in Haemaphysalis japonica,Haemaphysalis longicornis,Ixodes persulcatus,and Dermacentor silvarum.Conclusions:This study represents the first isolation and identification of WLTRV from H.concinna in the Yanbian Korean Autonomous Prefecture,providing new insights into the genetic diversity and evolution of the genus Coltivirus.展开更多
Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2...Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.Study selection Relevant articles on studies of Coltivirus in domestic and foreign literature were selected.Data extraction Data were maily extracted from the articles which are listed in the reference section of this review.Results Many Coltiviruses have been isolated not only from blood samples of patients with unknown fever or from cerebrospinal fluid of patients with encephalitis in Xishuangbanna area in Yunnan province, but also from mosquitoes collected in many areas in China. In some patients diagnosed as Japanese encephalitis or unknown fever, an increase of Coltivirus IgG antibody of fourfold, or more, has been detected using ELISA. Similarly, Coltivirus IgM antibody was positive in some patients with Japanese encephalitis or viral encephalitis. From most Chinese patients, except the northeastern, the isolates of Coltiviruses belong to subgroup B2, according to RT-PCR amplification of the ninth and twelfth segments of the isolates and sequence analysis of their amplicons. Some biological properties of Chinese Coltiviruses isolates are different from that of North American Coltiviruses.Conclusions The isolates of Coltiviruses from Chinese patients are one of the common agents causing viral encephalitis and unknown fever in summer-autumn season. It might be an important public health problem due to its high isolation rate and wide distribution in China. Mosquito is the main transmission vector of the virus.展开更多
基金supported by the Beijing Natural Science Foundation(No.L232069)the National Key R&D Program of China(No.2022YFC2302700)the Young Scholar Science Foundation of China CDC(No.2023A103).
文摘Backgroud:Coltiviruses are spherical,non-enveloped viruses with 12 double-stranded RNA segments,belonging to the family Spinareoviridae,and predominantly transmitted by ticks.This study isolated and characterized a novel coltivirus,designated Woodland tick reovirus(WLTRV),from Haemaphysalis concinna ticks collected in Helong City,Jilin Province,in Northeastern China.Methods SW-13 and Vero cells were used to isolate WLTRV through three blind passages,while seven mammalian cell lines assessed viral growth.Viral morphology was observed by electron microscopy.Next-generation sequencing,5ʹand 3ʹrapid amplification of cDNA ends were used to determine WLTRV whole genome sequences,and phylogenetic methods were used to characterize WLTRV.A real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect WLTRV RNA in ticks.Results:WLTRV grew and exerted cytopathic effects in human(SW-13 and 293T)and mouse(BHK-21 and N2A)cell lines,revealing its potential to infect mammals.Phylogenetic analysis based on RNA-dependent RNA polymerase sequences classified WLTRV within the genus Coltivirus,with a close evolutionary relationship to Tarumizu tick virus.The nucleotide and amino acid sequence homologies between WLTRV and Tarumizu tick virus across the 12 segments analyzed ranged from approximately 44.79%to 69.09%and 33.73%to 75.60%,respectively.WLTRV shared conserved the 5ʹ-terminal(GACAA/UU/A)and 3ʹ-terminal(UGCAGUC)consensus sequences of the genus Coltivirus genomes.Electron microscopy revealed WLTRV as spherical(diameter∼80 nm),non-enveloped,and morphologically consistent with coltiviruses.Among the 4,717 ticks collected from six towns in the Yanbian Korean Autonomous Prefecture,WLTRV RNA was only detected in H.concinna(0.95%virus-carrying rate)but not in Haemaphysalis japonica,Haemaphysalis longicornis,Ixodes persulcatus,and Dermacentor silvarum.Conclusions:This study represents the first isolation and identification of WLTRV from H.concinna in the Yanbian Korean Autonomous Prefecture,providing new insights into the genetic diversity and evolution of the genus Coltivirus.
文摘Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.Study selection Relevant articles on studies of Coltivirus in domestic and foreign literature were selected.Data extraction Data were maily extracted from the articles which are listed in the reference section of this review.Results Many Coltiviruses have been isolated not only from blood samples of patients with unknown fever or from cerebrospinal fluid of patients with encephalitis in Xishuangbanna area in Yunnan province, but also from mosquitoes collected in many areas in China. In some patients diagnosed as Japanese encephalitis or unknown fever, an increase of Coltivirus IgG antibody of fourfold, or more, has been detected using ELISA. Similarly, Coltivirus IgM antibody was positive in some patients with Japanese encephalitis or viral encephalitis. From most Chinese patients, except the northeastern, the isolates of Coltiviruses belong to subgroup B2, according to RT-PCR amplification of the ninth and twelfth segments of the isolates and sequence analysis of their amplicons. Some biological properties of Chinese Coltiviruses isolates are different from that of North American Coltiviruses.Conclusions The isolates of Coltiviruses from Chinese patients are one of the common agents causing viral encephalitis and unknown fever in summer-autumn season. It might be an important public health problem due to its high isolation rate and wide distribution in China. Mosquito is the main transmission vector of the virus.
