We develop a novel coumarin-all〈yne derivative （NC7-AL）, which can specifically react with Au^3＋ and give a colorimetric and fluorescent ＂turn-on＂ response toward Au3~, Notably, other alkynophilic metal species ...We develop a novel coumarin-all〈yne derivative （NC7-AL）, which can specifically react with Au^3＋ and give a colorimetric and fluorescent ＂turn-on＂ response toward Au3~, Notably, other alkynophilic metal species such as Au^＋, Ag^＋, Pd^2＋, Ni^2＋, Cu^2＋, and Hg^2＋ do not produce an interfering signal. A good linear relationship between emission intensity at 420 nm and Au^3＋ concentration from 0 to 2 equivalent is observed, and the detection limit （3σ/k） is estimated to be ca. 3.58 nmol/L. Harnessing the Au^3＋ .展开更多
Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO^- in water and biological samples is very meanin...Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO^- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe（Rh-ClO） based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO^- detection in water samples and HeLa cells. Upon addition of ClO^-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light（365 nm）. Rh-ClO displayed high selectivity and sensitivity for ClO^- , and the detection limit was 7 mmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO^- within 10 s, Rh-ClO and its test paper could successfully detect ClO^- in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO^- in water samples and in mitochondria of living cells.展开更多
Herein,a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1)in lumbar disc herniation(LDH)patients.Apo L1 could be easily found in hu...Herein,a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1)in lumbar disc herniation(LDH)patients.Apo L1 could be easily found in human serum of the LDH group,but not obviously expressed in the normal control group(Ctrl),spine spondylolisthesis(SSP)group,spinal fracture(SFR)group,and spine scoliosis(SSC)group.Furthermore,the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation.The P value of early diagnosis and recovery monitoring was<0.001 and>0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA),respectively,suggesting this detection strategy had significant differences compared with the traditional ELISA.All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH.This proposed detection strategy has a potential application in discovering new biomarkers of diseases.展开更多
A new rhodamine derivative,N-(3-carboxy)acryloyl rhodamine B hydrazide(CARB),has been synthesized,and its unusual spectroscopic reaction with Cu2+ has been investigated.The derivative exhibits a rapid and reversible n...A new rhodamine derivative,N-(3-carboxy)acryloyl rhodamine B hydrazide(CARB),has been synthesized,and its unusual spectroscopic reaction with Cu2+ has been investigated.The derivative exhibits a rapid and reversible non-fluorescent absorption upon coordination to Cu2+,which is a rather unusual phenomenon for rhodamine B derivatives.Stoichiometric measurements using the Job's method and the molar ratio method reveal that one CARB molecule combines two Cu2+ ions,and the two Cu2+ ions play different roles:one opens the spirocyclic structure and the other quenches the fluorescence of the xanthene moiety.This reaction mechanism is supported by a comparative study on the model compound N-acryloyl rhodamine B hydrazide as well as by the density functional theory calculations.Furthermore,the absorption response of CARB is highly selective for Cu2+ over other common ions,which implies that CARB may be used as a colorimetric probe for the rapid visual detection of Cu2+.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21102001, 21271035 and 21372005)Anhui Provincial Natural Science Foundation (No. 1608085MB39)+1 种基金Natural Science Foundation of Education Department of Anhui Province (Nos. KJ2015A047, KJ2014A013)211 Project of Anhui University
文摘We develop a novel coumarin-all〈yne derivative （NC7-AL）, which can specifically react with Au^3＋ and give a colorimetric and fluorescent ＂turn-on＂ response toward Au3~, Notably, other alkynophilic metal species such as Au^＋, Ag^＋, Pd^2＋, Ni^2＋, Cu^2＋, and Hg^2＋ do not produce an interfering signal. A good linear relationship between emission intensity at 420 nm and Au^3＋ concentration from 0 to 2 equivalent is observed, and the detection limit （3σ/k） is estimated to be ca. 3.58 nmol/L. Harnessing the Au^3＋ .
基金supported by the National Natural Science Foundation of China (No. 21302080)Program Funded by Liaoning Province Education Administration (No. L2014010)
文摘Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO^- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe（Rh-ClO） based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO^- detection in water samples and HeLa cells. Upon addition of ClO^-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light（365 nm）. Rh-ClO displayed high selectivity and sensitivity for ClO^- , and the detection limit was 7 mmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO^- within 10 s, Rh-ClO and its test paper could successfully detect ClO^- in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO^- in water samples and in mitochondria of living cells.
基金supported by the National Basic Research Program of China(2012CB933301)the Key Discipline Construction Project of Pudong Health Bureau of Shanghai(PWZx2014-02)+1 种基金the Sci-tech Support Plan of Jiangsu Province(BE2014719)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Herein,a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1)in lumbar disc herniation(LDH)patients.Apo L1 could be easily found in human serum of the LDH group,but not obviously expressed in the normal control group(Ctrl),spine spondylolisthesis(SSP)group,spinal fracture(SFR)group,and spine scoliosis(SSC)group.Furthermore,the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation.The P value of early diagnosis and recovery monitoring was<0.001 and>0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA),respectively,suggesting this detection strategy had significant differences compared with the traditional ELISA.All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH.This proposed detection strategy has a potential application in discovering new biomarkers of diseases.
基金supported by grants from the National Natural Science Foundation of China (20935005,90813032 & 20875092)National Basic Research Program of China (2010CB933502 & 2011CB935800)the Chinese Academy of Sciences
文摘A new rhodamine derivative,N-(3-carboxy)acryloyl rhodamine B hydrazide(CARB),has been synthesized,and its unusual spectroscopic reaction with Cu2+ has been investigated.The derivative exhibits a rapid and reversible non-fluorescent absorption upon coordination to Cu2+,which is a rather unusual phenomenon for rhodamine B derivatives.Stoichiometric measurements using the Job's method and the molar ratio method reveal that one CARB molecule combines two Cu2+ ions,and the two Cu2+ ions play different roles:one opens the spirocyclic structure and the other quenches the fluorescence of the xanthene moiety.This reaction mechanism is supported by a comparative study on the model compound N-acryloyl rhodamine B hydrazide as well as by the density functional theory calculations.Furthermore,the absorption response of CARB is highly selective for Cu2+ over other common ions,which implies that CARB may be used as a colorimetric probe for the rapid visual detection of Cu2+.
