bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the pres...bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the present study,a PdbHLH57 gene,belonging to the bHLH IIIf group,was characterized,which was isolated and cloned from the colored-leaf poplar‘Zhongshancaiyun’(ZSCY).The cDNA sequence of PdbHLH57 was 1887 base pairs,and the protein encoded by PdbHLH57 had 628 amino acids,the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa,respectively.Through bioinformatics analysis,PdbHLH57 has been classified into the IIIf bHLH subgroup,with many members of this subgroup known to participate in anthocyanin biosynthesis.The subcellular localization analysis conducted in the leaf protoplasts of‘ZSCY’revealed that the PdbHLH57 protein is specifically localized in the nucleus.The transcription activation analysis was also conducted,and the results showed that the PdbHLH57 protein had self-transcription activation.To better explore the functions of the PdbHLH57 protein,two parts of this protein(PdbHLH57-1,PdbHLH57-2)were split to detect their transcriptional activation activity.The results indicated that PdbHLH57-1(1-433aa)had self-transcription activation,and PdbHLH57-2(433-628aa)had no transcription activation.The expression of PdbHLH57 peaked in June during different developmental stages in‘ZSCY’,and it was most highly expressed in the phloem among various tissues.These findings offer a basis for understanding the role of PdbHLH57 in colored-leaf poplar.展开更多
Based on the investigation of colored-leaf plant resources in Hefei City,analytic hierarchy process(AHP)was used to construct a comprehensive evaluation system for colored-leaf plants in Hefei City.This system is comp...Based on the investigation of colored-leaf plant resources in Hefei City,analytic hierarchy process(AHP)was used to construct a comprehensive evaluation system for colored-leaf plants in Hefei City.This system is composed of three layers:leaf ornamental value,ecological adaptability,and other ornamental values,including 12 evaluation indicators.The landscape value of the 20 selected species of colored-leaf trees were comprehensively evaluated.The evaluation results show that 9 kinds of colored-leaf trees such as Ginkgo biloba and Tiadica sebifera are excellent(grade I,comprehensive evaluation value>3.5);7 species of colored-leaf trees such as Sophora japonica and Albizia juibissin are good(grade I 2.5≤comprehensive evaluation value≤3.5);4 types of colored-leaf trees such as Celtis sinensis and Pistacia chinensis are general(grade,comprehensive evaluation value<2.5).These results can provide theoretical references for the application of colored-leaf trees in uban green spaces of Hefei City.展开更多
[Objective] In this study, the relationship between the pigments and the color expression of leaves of colored-leaf plants was discussed. [Method] The colors of leaf blades of 6 kinds of plants were analyzed with the ...[Objective] In this study, the relationship between the pigments and the color expression of leaves of colored-leaf plants was discussed. [Method] The colors of leaf blades of 6 kinds of plants were analyzed with the Royal Horticultural Soci-ety Colour Chart. The chlorophyl content, carotenoids content and anthocyanin con-tent in leaf blades were determined. In addition, the color types of leaf blades, kinds of pigments, pigment contents and pigment distributions of 6 kinds of plants were compared. [Result] The chlorophyl contents ranked as Populus canadensis Moench （green leaves） 〉 Populus deltoids cv. Zhonghuahongye （purple green leaves） 〉 Populus euramericana cv. Quanhong （red leaves）; Acer palmatum Thunb. （green leaves） 〉 Acer palmatum cv. Atropurpureum （purple red leaves） 〉 Acer pal-matum Thunb. cv. Atropurpureum （red leaves）. The ranking of anthocyanin contents was just opposite. The chlorophyl content was negatively related to the anthocyanin content. The leaf color of plants is determined by various pigments. The more the chlorophyl is, the greener the leaf is. The more the anthocyanin is, the redder the leaf is. In the colored-leaf plants, the chlorophyl content represents about 80% of the content of pigments, the carotenoids content represents about 17%, and the an-thocyanin represents about 3%. There is a difference in the chlorophyl content be-tween colored-leaf plants and green plants. However, the relatively low chlorophyl content wil not hamper the normal life activities of colored-leaf plants. [Conclusion] We hoped to provide reference and basis for the production and landscaping of col-ored-leaf plants.展开更多
By using biological five points,expert consultation and fuzzy comprehensive evaluation,the species,phenological ornamental characteristics,and landscape application of colored-leaf trees in Lhasa City were surveyed sy...By using biological five points,expert consultation and fuzzy comprehensive evaluation,the species,phenological ornamental characteristics,and landscape application of colored-leaf trees in Lhasa City were surveyed systematically. The results show that there are 42 species of colored-leaf trees in Lhasa City,belonging to 31 genera and 18 families,of which there are 5,19,and 18 species of spring,autumn,and common colored-leaf trees,accounting for 11.90%,45.24% and 42.86% of total number of the investigated tree species respectively. It is clearly seen that there are too few species of spring colored-leaf trees in the city. There are 19 and 17 species of trees possessing red and yellow leaves,while the leaves of other 6 species of colored-leaf trees are shown in other colors,and the number of their species accounts for 45.24%,40.48% and 14.28% of total number of the investigated tree species respectively. The best ornamental periods of spring,autumn and common colored-leaf trees in Lhasa City average 38.4,41.8 and 251.8 d respectively. Prunus cerasifera Ehrhar f. atropurpurea Rehd.,Ulmus pumila L.,Populus × beijingensis W. Y. Hsu,and Salix alba are applied most frequently in Lhasa City,and their relative frequency is 18.67%,10.29%,9.91% and 8.95% respectively. According to the comprehensive assessment value of their ornamental characteristics,the ornamental characteristics of 15 species of colored-leaf trees in Lhasa City are good or very good,and there is a positive correlation between the comprehensive assessment value and relative frequency. Based on the investigation,the current application situation and problems of colored-leaf trees in Lhasa City were analyzed,and solutions to the problems were proposed.展开更多
For the quantitative analysis of colored-leaf plants in Tibet,the five-point sampling method is used for stratified investigation of garden plant communities in Tibet.(i)There are a total of 46 families,76 genera and ...For the quantitative analysis of colored-leaf plants in Tibet,the five-point sampling method is used for stratified investigation of garden plant communities in Tibet.(i)There are a total of 46 families,76 genera and 110 species of garden colored-leaf plants in Tibet,including 56 kinds of trees,40 kinds of bushes,9 kinds of herbs,4 kinds of vines and 1 kind of bamboo.There are too few colored-leaf herbs and bamboos,and there is a serious imbalance between evergreen and deciduous trees,between coniferous and broad leaved forests.(ii)The most widely applied families include Rosaceae(26),Salicaceae(12),Fabaceae(6),Aceraceae(5),Oleaceae(4)and Elaeagnaceae(4),accounting for 23.64%,10.91%,5.46%,4.55%,3.64% and 3.64% of the investigated colored-leaf plants,respectively.(iii)In terms of color,there are 55 kinds of red plants,43 kinds of yellow plants and 12 kinds of plants with other colors,accounting for 50.00%,39.09%and 10.91% of colored-leaf plants,respectively.There are 9 kinds of spring color leaf plants,63 kinds of autumn color leaf plants,29 kinds of constant color leaf plants,7 kinds of double color leaf plants and 2 kinds of spot color leaf plants,accounting for 8.18%,57.27%,26.36%,6.36% and 1.82% of colored-leaf plants,respectively,indicating that it is dominated by autumn color leaf and constant color leaf plants.(iv)In terms of importance value of trees,the top two are Salix alba(37.623)and Prunus cerasifera f.atropurpurea(26.063); in terms of importance value of bushes,the top three are Ligustrum × vicaryi Hort(22.577),Berberis thunbergii‘atropurpurea Nana'(18.987)and Platycladus orientalis Franco cv.Sieboldii(10.529); in terms of importance value of herbs,the top two are Taraxacum sherriffii(0.915)and Oxalis triangu laris cv.purpurea(0.326).(v)In terms of species abundance of colored-leaf plants,it is in the order of Nyingchi(94)> Lhasa(47)> Qamdo(43)> Shannan(34)> Xigaze(21)> Ali(7)> Nagqu(5).There are great differences between regions: it is highest in Nyingchi while it is lowest in Nagqu.Based on the main problems in the application of colored-leaf plants in Tibet,this paper makes the corresponding recommendations.展开更多
With the gradual improvement of living standard, the public demands on landscaping have focused more on quality than on quantity, and plant furnishing has grown from singular green plants to colored-leaf plants with m...With the gradual improvement of living standard, the public demands on landscaping have focused more on quality than on quantity, and plant furnishing has grown from singular green plants to colored-leaf plants with multiple layers. Confi guration of colored-leaf plants follows certain principles and patterns to enrich the beauty of plant landscaping, construct colorful urban green spaces, satisfy increasing application and aesthetic needs of the public, and reflect value of plant landscaping.展开更多
By investigating species, characteristics and landscaping application of colored-leaf species in Guang'an City, this paper proposed suggestions for the application of colored-leaf species in the local area.
基金supported by the Natural Science Foundation of Jiangsu Province,China(BK20242007)the Natural Science Foundation of China(32271916)the Jiangsu Agricultural Science and Technology Innovation Fund[CX(24)3048].
文摘bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the present study,a PdbHLH57 gene,belonging to the bHLH IIIf group,was characterized,which was isolated and cloned from the colored-leaf poplar‘Zhongshancaiyun’(ZSCY).The cDNA sequence of PdbHLH57 was 1887 base pairs,and the protein encoded by PdbHLH57 had 628 amino acids,the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa,respectively.Through bioinformatics analysis,PdbHLH57 has been classified into the IIIf bHLH subgroup,with many members of this subgroup known to participate in anthocyanin biosynthesis.The subcellular localization analysis conducted in the leaf protoplasts of‘ZSCY’revealed that the PdbHLH57 protein is specifically localized in the nucleus.The transcription activation analysis was also conducted,and the results showed that the PdbHLH57 protein had self-transcription activation.To better explore the functions of the PdbHLH57 protein,two parts of this protein(PdbHLH57-1,PdbHLH57-2)were split to detect their transcriptional activation activity.The results indicated that PdbHLH57-1(1-433aa)had self-transcription activation,and PdbHLH57-2(433-628aa)had no transcription activation.The expression of PdbHLH57 peaked in June during different developmental stages in‘ZSCY’,and it was most highly expressed in the phloem among various tissues.These findings offer a basis for understanding the role of PdbHLH57 in colored-leaf poplar.
基金Sponsored by the Provincial Innovation Training Project for College Students of Anhui Xinhua University in 2023(S202312216044)Key Research Project of Natural Science in Universities of Anhui Province(2023AH051816)General Teaching Research Project of Anhui Province(2022jyxm665).
文摘Based on the investigation of colored-leaf plant resources in Hefei City,analytic hierarchy process(AHP)was used to construct a comprehensive evaluation system for colored-leaf plants in Hefei City.This system is composed of three layers:leaf ornamental value,ecological adaptability,and other ornamental values,including 12 evaluation indicators.The landscape value of the 20 selected species of colored-leaf trees were comprehensively evaluated.The evaluation results show that 9 kinds of colored-leaf trees such as Ginkgo biloba and Tiadica sebifera are excellent(grade I,comprehensive evaluation value>3.5);7 species of colored-leaf trees such as Sophora japonica and Albizia juibissin are good(grade I 2.5≤comprehensive evaluation value≤3.5);4 types of colored-leaf trees such as Celtis sinensis and Pistacia chinensis are general(grade,comprehensive evaluation value<2.5).These results can provide theoretical references for the application of colored-leaf trees in uban green spaces of Hefei City.
基金Supported by the Technology Research and Development Program of Beijing Vocational College of Agriculture(XY-YF-13-39)~~
文摘[Objective] In this study, the relationship between the pigments and the color expression of leaves of colored-leaf plants was discussed. [Method] The colors of leaf blades of 6 kinds of plants were analyzed with the Royal Horticultural Soci-ety Colour Chart. The chlorophyl content, carotenoids content and anthocyanin con-tent in leaf blades were determined. In addition, the color types of leaf blades, kinds of pigments, pigment contents and pigment distributions of 6 kinds of plants were compared. [Result] The chlorophyl contents ranked as Populus canadensis Moench （green leaves） 〉 Populus deltoids cv. Zhonghuahongye （purple green leaves） 〉 Populus euramericana cv. Quanhong （red leaves）; Acer palmatum Thunb. （green leaves） 〉 Acer palmatum cv. Atropurpureum （purple red leaves） 〉 Acer pal-matum Thunb. cv. Atropurpureum （red leaves）. The ranking of anthocyanin contents was just opposite. The chlorophyl content was negatively related to the anthocyanin content. The leaf color of plants is determined by various pigments. The more the chlorophyl is, the greener the leaf is. The more the anthocyanin is, the redder the leaf is. In the colored-leaf plants, the chlorophyl content represents about 80% of the content of pigments, the carotenoids content represents about 17%, and the an-thocyanin represents about 3%. There is a difference in the chlorophyl content be-tween colored-leaf plants and green plants. However, the relatively low chlorophyl content wil not hamper the normal life activities of colored-leaf plants. [Conclusion] We hoped to provide reference and basis for the production and landscaping of col-ored-leaf plants.
基金Sponsored by National Natural Science Foundation of China(51568059)
文摘By using biological five points,expert consultation and fuzzy comprehensive evaluation,the species,phenological ornamental characteristics,and landscape application of colored-leaf trees in Lhasa City were surveyed systematically. The results show that there are 42 species of colored-leaf trees in Lhasa City,belonging to 31 genera and 18 families,of which there are 5,19,and 18 species of spring,autumn,and common colored-leaf trees,accounting for 11.90%,45.24% and 42.86% of total number of the investigated tree species respectively. It is clearly seen that there are too few species of spring colored-leaf trees in the city. There are 19 and 17 species of trees possessing red and yellow leaves,while the leaves of other 6 species of colored-leaf trees are shown in other colors,and the number of their species accounts for 45.24%,40.48% and 14.28% of total number of the investigated tree species respectively. The best ornamental periods of spring,autumn and common colored-leaf trees in Lhasa City average 38.4,41.8 and 251.8 d respectively. Prunus cerasifera Ehrhar f. atropurpurea Rehd.,Ulmus pumila L.,Populus × beijingensis W. Y. Hsu,and Salix alba are applied most frequently in Lhasa City,and their relative frequency is 18.67%,10.29%,9.91% and 8.95% respectively. According to the comprehensive assessment value of their ornamental characteristics,the ornamental characteristics of 15 species of colored-leaf trees in Lhasa City are good or very good,and there is a positive correlation between the comprehensive assessment value and relative frequency. Based on the investigation,the current application situation and problems of colored-leaf trees in Lhasa City were analyzed,and solutions to the problems were proposed.
基金Supported by Key Project of National Science and Technology Support Program(2013BAJ03B00)
文摘For the quantitative analysis of colored-leaf plants in Tibet,the five-point sampling method is used for stratified investigation of garden plant communities in Tibet.(i)There are a total of 46 families,76 genera and 110 species of garden colored-leaf plants in Tibet,including 56 kinds of trees,40 kinds of bushes,9 kinds of herbs,4 kinds of vines and 1 kind of bamboo.There are too few colored-leaf herbs and bamboos,and there is a serious imbalance between evergreen and deciduous trees,between coniferous and broad leaved forests.(ii)The most widely applied families include Rosaceae(26),Salicaceae(12),Fabaceae(6),Aceraceae(5),Oleaceae(4)and Elaeagnaceae(4),accounting for 23.64%,10.91%,5.46%,4.55%,3.64% and 3.64% of the investigated colored-leaf plants,respectively.(iii)In terms of color,there are 55 kinds of red plants,43 kinds of yellow plants and 12 kinds of plants with other colors,accounting for 50.00%,39.09%and 10.91% of colored-leaf plants,respectively.There are 9 kinds of spring color leaf plants,63 kinds of autumn color leaf plants,29 kinds of constant color leaf plants,7 kinds of double color leaf plants and 2 kinds of spot color leaf plants,accounting for 8.18%,57.27%,26.36%,6.36% and 1.82% of colored-leaf plants,respectively,indicating that it is dominated by autumn color leaf and constant color leaf plants.(iv)In terms of importance value of trees,the top two are Salix alba(37.623)and Prunus cerasifera f.atropurpurea(26.063); in terms of importance value of bushes,the top three are Ligustrum × vicaryi Hort(22.577),Berberis thunbergii‘atropurpurea Nana'(18.987)and Platycladus orientalis Franco cv.Sieboldii(10.529); in terms of importance value of herbs,the top two are Taraxacum sherriffii(0.915)and Oxalis triangu laris cv.purpurea(0.326).(v)In terms of species abundance of colored-leaf plants,it is in the order of Nyingchi(94)> Lhasa(47)> Qamdo(43)> Shannan(34)> Xigaze(21)> Ali(7)> Nagqu(5).There are great differences between regions: it is highest in Nyingchi while it is lowest in Nagqu.Based on the main problems in the application of colored-leaf plants in Tibet,this paper makes the corresponding recommendations.
基金Sponsored by National Social Science Foundation of China(14BZX084)Teachers’Professional Ethics Education and Research Base of Hunan Provincial Institutes of Higher Education
文摘With the gradual improvement of living standard, the public demands on landscaping have focused more on quality than on quantity, and plant furnishing has grown from singular green plants to colored-leaf plants with multiple layers. Confi guration of colored-leaf plants follows certain principles and patterns to enrich the beauty of plant landscaping, construct colorful urban green spaces, satisfy increasing application and aesthetic needs of the public, and reflect value of plant landscaping.
基金Sponsored by Research Program of Sichuan Provincial Department of Education(14ZB0390)
文摘By investigating species, characteristics and landscaping application of colored-leaf species in Guang'an City, this paper proposed suggestions for the application of colored-leaf species in the local area.
