OBJECTIVE To assess the functional change of SFRP1 （secreted frizzled-related protein1）, in colorectal tumorigenesis. METHODS Immunohistochemical investigation and the semiquantitative reverse transcription-polymera...OBJECTIVE To assess the functional change of SFRP1 （secreted frizzled-related protein1）, in colorectal tumorigenesis. METHODS Immunohistochemical investigation and the semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） were used to assess the expression of SFRP1, β-catenin （β-cat） and E-caderin （E-cad） in matched samples of normal colorectal mucosa, adenomas and cancers. RESULTS SFRP1 mRNA expression was down-regulated in the neoplasms, and abnormal expressions of β-cat and E-cad were found in colorectal adenomas and colorectal cancers. CONCLUSION Down-regulation of SFRP1 observed is consistent with its acting as a tumor suppressor gene in colorectal tumorigenesis.展开更多
Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens fr...Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens from polypus and colorectal carcinoma were examined with streptavidin-biotin peroxidase（S-P） immunohistochemical technique for the expressions of MGMT and Survivin. Results： We found that there were significant differences in MGMT and Survivin between polypus and colorectal carcinoma. Expression of MGMT was correlated with ages and lymph node metastasis while Survivin was associated with lymph node metastasis only. Meanwhile, the expression of MGMT was correlated with Survivin （P〈0.01, r=0.65）. But there was no significant difference between male and female and the different depth of infiltration. Conclusion： It is concluded that the abnormal expressions of MGMT and Survivin were associated with the degree of malignancy of colorectal tumor. They possibly could be useful indexes for the primary screening and prognosis of colorectal carcinoma. Examination of them may have an important guiding significance in the chemotherapy strategy.展开更多
文摘OBJECTIVE To assess the functional change of SFRP1 （secreted frizzled-related protein1）, in colorectal tumorigenesis. METHODS Immunohistochemical investigation and the semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） were used to assess the expression of SFRP1, β-catenin （β-cat） and E-caderin （E-cad） in matched samples of normal colorectal mucosa, adenomas and cancers. RESULTS SFRP1 mRNA expression was down-regulated in the neoplasms, and abnormal expressions of β-cat and E-cad were found in colorectal adenomas and colorectal cancers. CONCLUSION Down-regulation of SFRP1 observed is consistent with its acting as a tumor suppressor gene in colorectal tumorigenesis.
基金a grant from the Department of Sci & Tech of Hebei Province(No.03276117)
文摘Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens from polypus and colorectal carcinoma were examined with streptavidin-biotin peroxidase（S-P） immunohistochemical technique for the expressions of MGMT and Survivin. Results： We found that there were significant differences in MGMT and Survivin between polypus and colorectal carcinoma. Expression of MGMT was correlated with ages and lymph node metastasis while Survivin was associated with lymph node metastasis only. Meanwhile, the expression of MGMT was correlated with Survivin （P〈0.01, r=0.65）. But there was no significant difference between male and female and the different depth of infiltration. Conclusion： It is concluded that the abnormal expressions of MGMT and Survivin were associated with the degree of malignancy of colorectal tumor. They possibly could be useful indexes for the primary screening and prognosis of colorectal carcinoma. Examination of them may have an important guiding significance in the chemotherapy strategy.
