In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tend...In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tendency for high seed shattering in domesticated crops,such as rice,not only reduces paddy yield but also complicates mechanized harvesting.展开更多
In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.U...In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.Using pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU mutants.Wefound thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony formation.Interestingly,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony expansion.Cell-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and expansion.Thesefindings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control P.aeruginosa biofilm formation andvirulence.展开更多
基金supported by the National Natural Science Foundation of China(32372118 and 32188102)the Qian Qian Academician Workstation,China+3 种基金the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province,China(YSPTZX202303)the Nanfan Special Project,Chinese Academy of Agricultural Sciences(ZDXM2315)the Chinese Academy of Agricultural Sciences Talent Plan-Outstanding Young Talentthe Zhejiang Province’s High-level Talent Special Support Plan-Young Talent,China。
文摘In natural ecosystems,the timely abscission of seeds in wild plants is a crucial adaptive trait that contributes to reproductive success,population renewal,and colony expansion(Thurber et al.2010).In contrast,the tendency for high seed shattering in domesticated crops,such as rice,not only reduces paddy yield but also complicates mechanized harvesting.
基金Supported by the National Natural ScienceFoundation of China(12374206)to K.Z.National Key R&DProgram of China(2023YFC3402401)to K.Z.+2 种基金National KeyR&D Program of China(2020YFA0906900)to F.J.China,Postdoctoral Science Foundation(2023M740523)to J.Z.Sichuan Provincial People's Hospital Research Fund ofthe Academy(2022B1013)to J.Z。
文摘In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial behaviors.While PilU isconsidered a homolog of the TFP disassembling motor PilT,its specific roles remain unclear.Using pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU mutants.Wefound thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony formation.Interestingly,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony expansion.Cell-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and expansion.Thesefindings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control P.aeruginosa biofilm formation andvirulence.
