Colchicine is an anti-inflammatory alkaloid that reduces cardiovascular events through its actions on the interleukin(IL)-1β/IL-6/C-reactive protein pathway,which promotes the degradation and rupture of atherosclerot...Colchicine is an anti-inflammatory alkaloid that reduces cardiovascular events through its actions on the interleukin(IL)-1β/IL-6/C-reactive protein pathway,which promotes the degradation and rupture of atherosclerotic plaques.Low-dose colchicine(0.5 mg/day)has been shown to decrease major adverse car-diovascular events(MACE)by 31%among patients with stable atherosclerosis and 23%among those after a recent myocardial infarction.In patients with coronary artery disease(CAD)already taking a statin,colchicine in conjunction with lipid-lowering therapy has additionally been shown to provide a larger benefit with respect to secondary prevention of MACE.The drug is contrain-dicated in patients with renal or hepatic impairment and should be avoided in patients taking strong cytochrome P4503A4 or P-glycoprotein inhibitors.Low-dose colchicine was recently approved by the United States Food and Drug Administration in 2023 to reduce the risk of stroke,coronary revascularization,myocardial infarction,and cardiovascular death among patients with athero-sclerotic disease or multiple risk factors.This article focuses on the use of colchicine and its anti-inflammatory effects in preventing MACE among patients with CAD and patients without CAD with multiple risk factors.展开更多
Colchicine is one of the most widely used drugs in the world.While it is most commonly used in the treatment and prevention of gout,it is also widely used to treat other chronic inflammatory diseases,such as familial ...Colchicine is one of the most widely used drugs in the world.While it is most commonly used in the treatment and prevention of gout,it is also widely used to treat other chronic inflammatory diseases,such as familial Mediterranean fever and Behçet’s disease.Regarding cardiovascular disease,an established use of colchicine concerns pericarditis,both acute and chronic,and its effectiveness in this context is supported by multiple studies and robust evidence.Regarding coronary artery disease(CAD),colchicine use has been endorsed in both acute and chronic coronary syndromes(CCS),primarily because of two randomized controlled trials:The COLCOT trial for patients with acute coronary syndromes(ACS)and the LoDoCo2 trial for patients with CCS.Considering this robust evidence,CCS 2024 European Society of Cardiology(ESC)Guidelines recommended 0.5 mg daily colchicine in patients with atherosclerotic CAD to reduce the risk of myocardial infarction,stroke and need for revascularization.However,a few months after the publication of 2024 ESC Guidelines on CCS,the“CLEAR”trial demonstrated that among patients who had experienced an acute myocardial infarction,when initiated shortly after the event and continued for a median of 3 years,colchicine did not reduce the incidence of the composite outcome of death from cardiovascular causes,recurrent myocardial infarction,stroke,or unplanned ischemia-driven coronary revascularization.This result casts doubt on the indication for colchicine use in ACS and weakens evidence that had previously led to the routine use of colchicine in clinical cardiology practice.This review aims to shed light on the current and past scientific evidence underlying the use of colchicine in ACS,CCS and cerebrovascular disease,and thus seeks to provide a quick yet effective tool for cardiologists facing the long-standing issue of reducing residual inflammatory risk in patients with coronary atherosclerotic disease.展开更多
[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotu...[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotus. [Result] The results showed that the concentration of 0.2% colchicine solution could induced double sweet clover which showed corresponding features of polyploidy plants as a whole showed great features. [Conclusion] Sweet clover induced by colchicine was polyploidy.展开更多
AIM:To test whether colchicine would be an effective antif ibrotic agent for treatment of chronic liver diseases in patients who could not be treated with α-interferon.METHODS:Seventy-four patients(46 males,28 female...AIM:To test whether colchicine would be an effective antif ibrotic agent for treatment of chronic liver diseases in patients who could not be treated with α-interferon.METHODS:Seventy-four patients(46 males,28 females) aged 40-66 years(mean 53±13 years) participated in the study.The patients were affected by chronic liver diseases with cirrhosis which was proven histologically(n=58);by chronic active hepatitis C(n=4),chronic active hepatitis B(n=2),and chronic persistent hepatitis C(n=6).In the four patients lacking histology,cirrhosis was diagnosed from anamnesis,serum laboratory tests,esophageal varices and ascites.Patients were assigned to colchicine(1 mg/d) or standard treatment as control in a randomized,double-blind trial,and followed for 4.4 years with clinical and laboratory evaluation.RESULTS:Survival at the end of the study was 94.6% in the colchicine group and 78.4% in the control group(P=0.001).Serum N-terminal peptide of type Ⅲ procollagen levels fell from 34.0 to 18.3 ng/mL(P=0.0001),and pseudocholinesterase levels rose from 4.900 to 5.610 mU/mL(P=0.0001) in the colchicine group,while no signif icant change was seen in controls.Best results were obtained in patients with chronic hepatitis C and in alcoholic cirrhotics.CONCLUSION:Colchicine is an effective and safe antifibrotic drug for long-term treatment of chronic liver disease in which fi brosis progresses towards cirrhosis.展开更多
In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for n...In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.展开更多
To search for more potent antitumor agent, a series of novel nitric oxide-donating colchicine （Col） derivatives （6a-f, 8a and b） were synthesized by coupling nitrates with N-methyl colchiceinamide. Their cytotoxic...To search for more potent antitumor agent, a series of novel nitric oxide-donating colchicine （Col） derivatives （6a-f, 8a and b） were synthesized by coupling nitrates with N-methyl colchiceinamide. Their cytotoxicity against four human cancer cell lines in vitro were evaluated by MTT assay. It was found that many of the derivatives displayed significant activity, particularly, compounds 6c, 8a and 8b showed more potent cytotoxic activities than Col.展开更多
[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extract...[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S. sagittifolia were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal test was carried out to optimize the extraction process. According to the optimal extraction process, the content of colchicine in S. sagittifolia was determined by high performance liquid chromatography. The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro. [Result] The optimal extraction process of colchicine from S. sagittifolia was as follows： ethanol concentration of 60%, extraction temper- ature of 50℃, extraction time of 30 min, and solid-liquid ratio of 1：25 （g/ml）. The content of colchicine in S. sagittifolia was determined as 40.58 μg/100 mg. Com- pared with the control, the colchicine from S. sagittifolia （9.0 and 4.5 g/kg） inhibited the increase in PGE2, TNF-α and IL-1β contents in pleural fluid （P〈0.05）. High-dose colchicine inhibited the increase in TNF-α, IL-1β and MDA contents in lung tissue （P〈0.01）, and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue （P〈0.01）. [Conclusion] The colchicine in S. sagittifolia has a good anti-inflam- matory effect, which may be achieved through hindering the production of inflam- matory mediators and antioxidation.展开更多
AIM To develop a novel rat model of heterogeneous hepatic injury.METHODS Seventy male Sprague-Dawley rats were randomly divided into a control group(n = 10) and a colchicine group(n =60). A 0.25% colchicine solution(0...AIM To develop a novel rat model of heterogeneous hepatic injury.METHODS Seventy male Sprague-Dawley rats were randomly divided into a control group(n = 10) and a colchicine group(n =60). A 0.25% colchicine solution(0.4 mL/kg) was injected via the splenic vein in the colchicine group to develop a rat model of heterogeneous hepatic injury. An equal volume of normal saline was injected via the splenic vein in the control group. At days 3, 7, and 14 and weeks 4, 8, and 12 after the operation, at least seven rats of the colchicine group were selected randomly for magnetic resonance imaging(MRI) examinations, and then they were euthanized. Ten rats of the control group underwent MRI examinations at the same time points, and then were euthanized at week 12. T2-weighted images(T2 WI) and diffusion weighted imaging(DWI) were used to evaluate the heterogeneous hepatic injury. The heterogeneous injury between the left and right hepatic lobes was assessed on liver sections according to the histological scoring criteria, and correlated with the results of MRI study. RESULTS Obvious pathological changes occurred in the hepatic parenchyma in the colchicine group. Hepatic injury scores were significantly different between the left and right lobes at each time point(P < 0.05). There was a significant difference in apparent diffusion coefficient(ADC) of DWI and liver-to-muscle ratio(LMR) of T2 WI between the left and right lobes of rats in the colchicine group(P < 0.05) at each time point, and similar results were observed between the colchicine and control groups. Besides, there was a significant correlation between hepatic injury scores and ADC values or LMR(r =-0.682, P = 0.000; r =-0.245, P = 0.018).CONCLUSION Injection with colchicine via the splenic vein can be used to successfully develop a rat model of heterogeneous hepatic injury. DWI and T2 WI may help evaluate the heterogeneous injury among liver lobes.展开更多
Objective: The current study evaluated various new colchicine analogs for their anticancer activity and to study the primary mechanism of apoptosis and in vivo antitumor activity of the analogs with selective anticanc...Objective: The current study evaluated various new colchicine analogs for their anticancer activity and to study the primary mechanism of apoptosis and in vivo antitumor activity of the analogs with selective anticancer properties and minimal toxicity to normal cells.Methods: Sulforhodamine B(SRB) assay was used to screen various colchicine analogs for their in vitro cytotoxicity. The effect of N-[(7S)-1,2,3-trimethoxy-9-oxo-10-(pyrrolidine-1-yl)5,6,7,9-tetrahydrobenzo[a] heptalene-7-yl] acetamide(ⅢM-067) on clonogenicity, apoptotic induction, and invasiveness of A549 cells was determined using a clonogenic assay, scratch assay, and staining with 4’,6-diamidino-2-phenylindole(DAPI) and annexin V/propidium iodide. Mitochondrial membrane potential(MMP) and reactive oxygen species(ROS) levels were observed using fluorescence microscopy. Western blot analysis was used to quantify expression of proteins involved in apoptosis, cell cycle, and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling. Pharmacokinetic and in vivo efficacy studies against Ehrlich ascites carcinoma(EAC) and Ehrlich solid tumor models were conducted using Swiss albino mice.Results: ⅢM-067 showed potent cytotoxicity and better selectivity than all other colchicine analogs screened in this study. The selective activity of ⅢM-067 toward A549 cells was higher among other cancer cell lines,with a selectivity index(SI) value of 2.28. ⅢM-067 demonstrated concentration-and time-dependent cytotoxicity against A549 cells with half-maximal inhibitory concentration values of 0.207, 0.150 and0.106 μmol/L at 24, 48 and 72 h, respectively. It also had reduced toxicity to normal cells(SI > 1) than the parent compound colchicine(SI = 1). ⅢM-067 reduced the clonogenic ability of A549 cells in a dose-dependent manner. ⅢM-067 enhanced ROS production from 24.6% at 0.05 μmol/L to 82.1% at 0.4 μmol/L and substantially decreased the MMP(100% in control to 5.6% at 0.4 μmol/L). The annexin V-FITC assay demonstrated78% apoptosis at 0.4 μmol/L. ⅢM-067 significantly(P < 0.5) induced the expression of various intrinsic apoptotic pathway proteins, and it differentially regulated the PI3K/AKT/mTOR signaling pathway. Furthermore,ⅢM-067 exhibited remarkable in vivo anticancer activity against the murine EAC model, with tumor growth inhibition(TGI) of 67.0% at a dose of 6 mg/kg(i.p.) and a reduced mortality compared to colchicine. ⅢM-067also effectively inhibited the tumor growth in the murine solid tumor model with TGI rates of 48.10%, 55.68%and 44.00% at doses of 5 mg/kg(i.p.), 6 mg/kg(i.p.) and 7 mg/kg(p.o.), respectively.Conclusion: ⅢM-067 exhibited significant anticancer activity with reduced toxicity both in vitro and in vivo and is a promising anticancer candidate. However, further studies are required in clinical settings to fully understand its potential.展开更多
A variety of plants were colchicine treated to double their chromosome number.Chromosomes are genetic carriers that determine the individual traits of organisms.The doubling of chromosomes will lead to modifications i...A variety of plants were colchicine treated to double their chromosome number.Chromosomes are genetic carriers that determine the individual traits of organisms.The doubling of chromosomes will lead to modifications in plant morphology,physiology and genetics.To determine the response of mulberry trees induced by colchicine,using mulberry variety Yu-711 leaves as research materials,two small RNA libraries(control and experimental groups)were constructed.It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results.A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA genes,19 genes of which were up-regulated and 18 genes of which were down-regulated.Eight miRNAs were selected from 37 differentially expressed miRNA genes.These miRNAs were verified by quantitative real-time PCR,and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing,which proved the accuracy of sequencing results.The PsRNATarget software was used to predict the target genes of 8 miRNAs.The Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect the functions of target genes and confirm that target genes are mainly involved in biological processes,cell components and molecular functions.novel-77 miRNA was selected from 37 differentially expressed miRNAs.What will serve as a fundamental data in understanding mulberry miRNAs function in molecular biology research and further provides the molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77 precursor sequence and the target gene(XM_010104258.2),as well as the secondary structure analysis.展开更多
Acute and recurring pericarditis are frequently encountered clinical entities.Given that severe complications such as tamponade and constrictive pericarditis occur rarely,the majority of patients suffering from acute ...Acute and recurring pericarditis are frequently encountered clinical entities.Given that severe complications such as tamponade and constrictive pericarditis occur rarely,the majority of patients suffering from acute pericarditis will have a benign clinical course.However,pericarditis recurrence,with its painful symptoms,is frequent.In effect,recent studies have demonstrated a beneficial role of colchicine in preventing recurrence,while also suggesting an increase in recurrences with the use of corticosteroids,the traditional first-line agent.展开更多
AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/...AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/VGH cells were irradiated at various doses （0, 1, 2, 4, and 8 Gy）. Colony assay was performed to assess the surviving cell fraction. Survival curves were fitted by using a linear-quadratic model to estimate the sensitizer enhancement ratio （SER）. Flow cytometry was used for cell cycle analysis.RESULTS： Colchicine at lower concentrations （1 and 2 ng/mL） had obvious synergy with radiation to inhibit HCC cell growth, whereas higher concentrations （4 and 8 ng/mL） had only additive effect to radiation. Pretreatment with 1 and 2 ng/mL colchicine for 24-h enhanced cell killing by radiation with SERs of 1.21 and 1.53, respectively.G2/N arrest was only observed with higher colchicine doses （8 and 16 ng/mL） after 24-h treatment, this effect was neither seen with lower doses （1, 2, and 4 ng/mL）nor with any dose after only 1 h of treatment.CONCLUSION： Our results suggest that colchicine has potential as an adjunct to radiotherapy for HCC treatment.Lower doses of colchicine possess radiosensitizing effects via some mechanism other than G2/M arrest. Further study is necessary to elucidate the mechanism.展开更多
Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were an...Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide mieroarray. Colehieine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colehicine, the expression of tissue inhibitor of metalloprotenase-1 （TIMP-1） and the expression of interleukin-6 （IL-6） in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchieine＇s beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down regulation of the expression of both TIMP1 and IL-6 in HSCs.展开更多
An induced polyploid plant through colchicine treatment offers probably the best scope for improvement in flower size and fruit weight.Thus,in the present study,an attempt was made to induce polyploidy in Cape goosebe...An induced polyploid plant through colchicine treatment offers probably the best scope for improvement in flower size and fruit weight.Thus,in the present study,an attempt was made to induce polyploidy in Cape gooseberry using colchicine with the objective of creating more genetic variability.The colchicine concentrations were used as 0.10%(C1),0.20%(C2)and 0.40%(C3)for the duration 12(H1),24(H2)and 36(H3)hours for each concentration with seedling apex dip method(M1),cotton plug method(M2)and lanolin paste method(M3).The plants treated with 0.10%of colchicine by cotton plug method for 12 h showed the better performance during the years 2017-2018 and 2018-2019 in respect of more delay in the flower bud emergence(54 d and 53 d from the date of transplanting),anthesis(19 d and 20 d from the first appearance of bud to full anthesis of flower)and fruit setting(8.00 d and 9.00 d from the date of anthesis),bigger flower size(2.93 cm2 and 3.00 cm2)than the untreated plants.The lower percentage of pollen viability(40.33%and 40.67%)was noticed in the same treatment in comparison to control(70.33%and 72.33%).The fruit maturity was also extended(59 d and 60 d from the date of fruit set)with bigger sized fruits(length:2.53 cm and 2.57 cm,breadth:2.27 cm and 2.33 cm)as well as more fruit weight(8.70 g and 8.33 g)by the application of colchicine at 0.10%with cotton plug method for 12 h.On the basis of results obtained,the application of colchicine at 0.10%for 12 h duration with cotton plug method was found to be the best and effective treatment for induction of polyploidy as well as more flower size and fruit weight in Cape gooseberry.展开更多
The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for ...The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for their production by in vitro plant cell and tissue cultures have been accelerated considerably. Colchicine, the principle secondary metabolite of Colchicum autumnale L. and Gloriosa superba L., is an important alkaloid that has poison effect used for treatment of various diseases and plant breeding studies. Presently, colchicine has been produced by using the seeds of C. autumnale L. and the tubers of G. superba L. through different chemical extraction methods. Applying in vitro plant cell and tissue cultures together with metabolic and genetic engineering techniques, large-scale production of colchicine can be achieved from the above two plant species.展开更多
The distribution of mitochondria during early development of mouse embryos was visualized bymitochondria-specific vital fluorescent dye, rhodamine 123(Rh 123). Mitochondrial clusters wasmarkedly conceotrated to perinu...The distribution of mitochondria during early development of mouse embryos was visualized bymitochondria-specific vital fluorescent dye, rhodamine 123(Rh 123). Mitochondrial clusters wasmarkedly conceotrated to perinuclear area in blastomere of normal 2-ccll embryos. In blastomere ofuncompacted 8-cell embryos, mitochondria were randomly distributed throughout the cytoplasm, butthey were reorganizcd to the cytocortices beneath the apposed surfaces of blastomere duringcompaction. As demonstrated in our study, colchicine (10 μg/ml) produced marked effect onmitochondrial distribution in blastomcre of 2-cell and compacted 8-cell embryos: mitochondriabecame scattered throughout the cytoplasm ofblastomere. It is suggested that the spatial distributionof mitochondria in early mouse embryo are maintained by microtubule.展开更多
In order to avoid any possible effect of separating procedures from intact cell on morphologic structure of the chromosomes, cultivated Hep-2 tumor cells were treated with colchicine and observed by soft X-ray contact...In order to avoid any possible effect of separating procedures from intact cell on morphologic structure of the chromosomes, cultivated Hep-2 tumor cells were treated with colchicine and observed by soft X-ray contact microscopy for the first time. The fine structures of chromosomes are more clear with stereo features. The thread-like and coarse granular structures twine and tangle up together within chromosome masses which can not easily be revealed in Wright’s stained sample by light microscope or osmium stained sample by transmission electron展开更多
OBJECTIVE： To establish and optimize the propaga- tion of Nianmaohuangqin （Radix Scutellariae Viscid- ulae） and induce and characterize polyploidy of Nianmaohuangqin （Radix Scutellariae Viscidulae）. METHODS： Bud...OBJECTIVE： To establish and optimize the propaga- tion of Nianmaohuangqin （Radix Scutellariae Viscid- ulae） and induce and characterize polyploidy of Nianmaohuangqin （Radix Scutellariae Viscidulae）. METHODS： Buds from germinating seed-derived explants were induced by tissue culture. With an or- thogonal test, different concentrations of 6-benzyl- aminopurine （BAP）, indole-3-acetic acid （IAA） and kinetin （KT） were used to determine the optimal concentrations for the propagation of Nianmaohuangqin （Radix Scutellariae Viscidulae）. The differ- ent concentrations of IAA and rooting powder （ABT） were used to induce rooting. A 0.3% w/v col- chicine solution was used to induce polyploidy and the induced buds was identified by root-tip chromosome determination and stomatal apparatus ob- servation. RESULTS： A large number of buds could be in- duced directly from epicotyl and hypocotyl ex- plants on Murashige and Skoog （MS） （Murashige and Skoog 1962） medium supplemented with 1.1-1.3 mg/L BAP and 0.2 mg/L IAA. Root induction and development could be observed within 20 days of inoculation on 1/2 MS medium supplemented with 0.2 mg/L IAA and 0.1 mg/L ABTo Furthermore, 27 lines of autotetraploid individuals were ob- tained with a plantlet chromosome number of 2n= 4x=36. CONCLUSION： Autotetraploid lines could be ob- tained through induction with colchicine in vitro, proving that this method might be used for plant selection and breeding.展开更多
Colchicine has been widely used as an anti-gout medication over the past decades.However,it is less commonly used due to its narrow therapeutic range,meaning that its lethal dose is close to its therapeutic dose.The l...Colchicine has been widely used as an anti-gout medication over the past decades.However,it is less commonly used due to its narrow therapeutic range,meaning that its lethal dose is close to its therapeutic dose.The lethal dose of colchicine is considered to be 0.8 mg/kg.As chronic colchicine poisoning has multiple manifestations,it poses a challenge in the clinician’s differential diagnosis.Historically,the drug was important in treating gout;however,clinical studies are currently underway regarding the use of colchicine in patients with coronavirus disease 2019 as well as its use in coronary artery disease,making this drug more important in clinical practice.CASE SUMMARY A 61-year-old male with a history of gout and chronic colchicine intake was admitted to our Emergency Department due to numbness and weakness of the lower limbs.The patient reported a history of colchicine intake for 23 years.After thorough examination,he was diagnosed with colchicine poisoning,manifesting as neuromyopathy,multiple gastric ulcers and myelosuppression.We advised him to stop taking colchicine and drinking alcohol.We also provided a prescription of lansoprazole and mecobalamin,and then asked him to return to the clinic for re-examination.The patient was followed up for 3-mo during which time his gout symptoms were controlled to the point where he was asymptomatic.CONCLUSION Colchicine overdose can mimic the clinical manifestations of several conditions.Physicians easily pay attention to the disease while ignoring the cause of the disease.Thus,the patient’s medication history should never be ignored.展开更多
Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects ind...Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.展开更多
文摘Colchicine is an anti-inflammatory alkaloid that reduces cardiovascular events through its actions on the interleukin(IL)-1β/IL-6/C-reactive protein pathway,which promotes the degradation and rupture of atherosclerotic plaques.Low-dose colchicine(0.5 mg/day)has been shown to decrease major adverse car-diovascular events(MACE)by 31%among patients with stable atherosclerosis and 23%among those after a recent myocardial infarction.In patients with coronary artery disease(CAD)already taking a statin,colchicine in conjunction with lipid-lowering therapy has additionally been shown to provide a larger benefit with respect to secondary prevention of MACE.The drug is contrain-dicated in patients with renal or hepatic impairment and should be avoided in patients taking strong cytochrome P4503A4 or P-glycoprotein inhibitors.Low-dose colchicine was recently approved by the United States Food and Drug Administration in 2023 to reduce the risk of stroke,coronary revascularization,myocardial infarction,and cardiovascular death among patients with athero-sclerotic disease or multiple risk factors.This article focuses on the use of colchicine and its anti-inflammatory effects in preventing MACE among patients with CAD and patients without CAD with multiple risk factors.
文摘Colchicine is one of the most widely used drugs in the world.While it is most commonly used in the treatment and prevention of gout,it is also widely used to treat other chronic inflammatory diseases,such as familial Mediterranean fever and Behçet’s disease.Regarding cardiovascular disease,an established use of colchicine concerns pericarditis,both acute and chronic,and its effectiveness in this context is supported by multiple studies and robust evidence.Regarding coronary artery disease(CAD),colchicine use has been endorsed in both acute and chronic coronary syndromes(CCS),primarily because of two randomized controlled trials:The COLCOT trial for patients with acute coronary syndromes(ACS)and the LoDoCo2 trial for patients with CCS.Considering this robust evidence,CCS 2024 European Society of Cardiology(ESC)Guidelines recommended 0.5 mg daily colchicine in patients with atherosclerotic CAD to reduce the risk of myocardial infarction,stroke and need for revascularization.However,a few months after the publication of 2024 ESC Guidelines on CCS,the“CLEAR”trial demonstrated that among patients who had experienced an acute myocardial infarction,when initiated shortly after the event and continued for a median of 3 years,colchicine did not reduce the incidence of the composite outcome of death from cardiovascular causes,recurrent myocardial infarction,stroke,or unplanned ischemia-driven coronary revascularization.This result casts doubt on the indication for colchicine use in ACS and weakens evidence that had previously led to the routine use of colchicine in clinical cardiology practice.This review aims to shed light on the current and past scientific evidence underlying the use of colchicine in ACS,CCS and cerebrovascular disease,and thus seeks to provide a quick yet effective tool for cardiologists facing the long-standing issue of reducing residual inflammatory risk in patients with coronary atherosclerotic disease.
基金Supported by General Project of Education Department in Helongjiang (11531263)Key Project of Science and Technology Bureau, Helongjiang Land Reclamation Bureau (HNKXIV-08-06-09)~~
文摘[Objective] The aim was to identify the ploidy of sweet clover induced by colchicine. [Method] The sweet clover germinating seeds and colchicine solution were used for materials to research colchicine-induced Melilotus. [Result] The results showed that the concentration of 0.2% colchicine solution could induced double sweet clover which showed corresponding features of polyploidy plants as a whole showed great features. [Conclusion] Sweet clover induced by colchicine was polyploidy.
文摘AIM:To test whether colchicine would be an effective antif ibrotic agent for treatment of chronic liver diseases in patients who could not be treated with α-interferon.METHODS:Seventy-four patients(46 males,28 females) aged 40-66 years(mean 53±13 years) participated in the study.The patients were affected by chronic liver diseases with cirrhosis which was proven histologically(n=58);by chronic active hepatitis C(n=4),chronic active hepatitis B(n=2),and chronic persistent hepatitis C(n=6).In the four patients lacking histology,cirrhosis was diagnosed from anamnesis,serum laboratory tests,esophageal varices and ascites.Patients were assigned to colchicine(1 mg/d) or standard treatment as control in a randomized,double-blind trial,and followed for 4.4 years with clinical and laboratory evaluation.RESULTS:Survival at the end of the study was 94.6% in the colchicine group and 78.4% in the control group(P=0.001).Serum N-terminal peptide of type Ⅲ procollagen levels fell from 34.0 to 18.3 ng/mL(P=0.0001),and pseudocholinesterase levels rose from 4.900 to 5.610 mU/mL(P=0.0001) in the colchicine group,while no signif icant change was seen in controls.Best results were obtained in patients with chronic hepatitis C and in alcoholic cirrhotics.CONCLUSION:Colchicine is an effective and safe antifibrotic drug for long-term treatment of chronic liver disease in which fi brosis progresses towards cirrhosis.
基金the National Natural Science Foundation of China,No. 30700436
文摘In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.
文摘To search for more potent antitumor agent, a series of novel nitric oxide-donating colchicine （Col） derivatives （6a-f, 8a and b） were synthesized by coupling nitrates with N-methyl colchiceinamide. Their cytotoxicity against four human cancer cell lines in vitro were evaluated by MTT assay. It was found that many of the derivatives displayed significant activity, particularly, compounds 6c, 8a and 8b showed more potent cytotoxic activities than Col.
基金Supported by Scientific Research Foundation of the Education Department of Sichuan Province,China~~
文摘[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from Sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S. sagittifolia were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal test was carried out to optimize the extraction process. According to the optimal extraction process, the content of colchicine in S. sagittifolia was determined by high performance liquid chromatography. The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro. [Result] The optimal extraction process of colchicine from S. sagittifolia was as follows： ethanol concentration of 60%, extraction temper- ature of 50℃, extraction time of 30 min, and solid-liquid ratio of 1：25 （g/ml）. The content of colchicine in S. sagittifolia was determined as 40.58 μg/100 mg. Com- pared with the control, the colchicine from S. sagittifolia （9.0 and 4.5 g/kg） inhibited the increase in PGE2, TNF-α and IL-1β contents in pleural fluid （P〈0.05）. High-dose colchicine inhibited the increase in TNF-α, IL-1β and MDA contents in lung tissue （P〈0.01）, and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue （P〈0.01）. [Conclusion] The colchicine in S. sagittifolia has a good anti-inflam- matory effect, which may be achieved through hindering the production of inflam- matory mediators and antioxidation.
基金Supported by the Chinese National Natural Science Foundation,No.81471719
文摘AIM To develop a novel rat model of heterogeneous hepatic injury.METHODS Seventy male Sprague-Dawley rats were randomly divided into a control group(n = 10) and a colchicine group(n =60). A 0.25% colchicine solution(0.4 mL/kg) was injected via the splenic vein in the colchicine group to develop a rat model of heterogeneous hepatic injury. An equal volume of normal saline was injected via the splenic vein in the control group. At days 3, 7, and 14 and weeks 4, 8, and 12 after the operation, at least seven rats of the colchicine group were selected randomly for magnetic resonance imaging(MRI) examinations, and then they were euthanized. Ten rats of the control group underwent MRI examinations at the same time points, and then were euthanized at week 12. T2-weighted images(T2 WI) and diffusion weighted imaging(DWI) were used to evaluate the heterogeneous hepatic injury. The heterogeneous injury between the left and right hepatic lobes was assessed on liver sections according to the histological scoring criteria, and correlated with the results of MRI study. RESULTS Obvious pathological changes occurred in the hepatic parenchyma in the colchicine group. Hepatic injury scores were significantly different between the left and right lobes at each time point(P < 0.05). There was a significant difference in apparent diffusion coefficient(ADC) of DWI and liver-to-muscle ratio(LMR) of T2 WI between the left and right lobes of rats in the colchicine group(P < 0.05) at each time point, and similar results were observed between the colchicine and control groups. Besides, there was a significant correlation between hepatic injury scores and ADC values or LMR(r =-0.682, P = 0.000; r =-0.245, P = 0.018).CONCLUSION Injection with colchicine via the splenic vein can be used to successfully develop a rat model of heterogeneous hepatic injury. DWI and T2 WI may help evaluate the heterogeneous injury among liver lobes.
基金Sumera Malik acknowledges the University Grants Commission for senior research fellowship(GAP-1128)Dr.Sanket K.Shukla acknowledges the SERB-DST for Ramanujan Fellowship(SB/S2/RJN-078/2019)and project(GAP-2194).
文摘Objective: The current study evaluated various new colchicine analogs for their anticancer activity and to study the primary mechanism of apoptosis and in vivo antitumor activity of the analogs with selective anticancer properties and minimal toxicity to normal cells.Methods: Sulforhodamine B(SRB) assay was used to screen various colchicine analogs for their in vitro cytotoxicity. The effect of N-[(7S)-1,2,3-trimethoxy-9-oxo-10-(pyrrolidine-1-yl)5,6,7,9-tetrahydrobenzo[a] heptalene-7-yl] acetamide(ⅢM-067) on clonogenicity, apoptotic induction, and invasiveness of A549 cells was determined using a clonogenic assay, scratch assay, and staining with 4’,6-diamidino-2-phenylindole(DAPI) and annexin V/propidium iodide. Mitochondrial membrane potential(MMP) and reactive oxygen species(ROS) levels were observed using fluorescence microscopy. Western blot analysis was used to quantify expression of proteins involved in apoptosis, cell cycle, and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling. Pharmacokinetic and in vivo efficacy studies against Ehrlich ascites carcinoma(EAC) and Ehrlich solid tumor models were conducted using Swiss albino mice.Results: ⅢM-067 showed potent cytotoxicity and better selectivity than all other colchicine analogs screened in this study. The selective activity of ⅢM-067 toward A549 cells was higher among other cancer cell lines,with a selectivity index(SI) value of 2.28. ⅢM-067 demonstrated concentration-and time-dependent cytotoxicity against A549 cells with half-maximal inhibitory concentration values of 0.207, 0.150 and0.106 μmol/L at 24, 48 and 72 h, respectively. It also had reduced toxicity to normal cells(SI > 1) than the parent compound colchicine(SI = 1). ⅢM-067 reduced the clonogenic ability of A549 cells in a dose-dependent manner. ⅢM-067 enhanced ROS production from 24.6% at 0.05 μmol/L to 82.1% at 0.4 μmol/L and substantially decreased the MMP(100% in control to 5.6% at 0.4 μmol/L). The annexin V-FITC assay demonstrated78% apoptosis at 0.4 μmol/L. ⅢM-067 significantly(P < 0.5) induced the expression of various intrinsic apoptotic pathway proteins, and it differentially regulated the PI3K/AKT/mTOR signaling pathway. Furthermore,ⅢM-067 exhibited remarkable in vivo anticancer activity against the murine EAC model, with tumor growth inhibition(TGI) of 67.0% at a dose of 6 mg/kg(i.p.) and a reduced mortality compared to colchicine. ⅢM-067also effectively inhibited the tumor growth in the murine solid tumor model with TGI rates of 48.10%, 55.68%and 44.00% at doses of 5 mg/kg(i.p.), 6 mg/kg(i.p.) and 7 mg/kg(p.o.), respectively.Conclusion: ⅢM-067 exhibited significant anticancer activity with reduced toxicity both in vitro and in vivo and is a promising anticancer candidate. However, further studies are required in clinical settings to fully understand its potential.
基金This work was supported by China Agriculture Research System of MOF and MARA,National Key R&D Program of China,Key Projects of International Scientific and Technological Innovation Cooperation(2021YFE0111100)Guangxi Innovation-Driven Development Project(AA19182012-2)Zhenjiang Science and Technology Support Project(GJ2021015).
文摘A variety of plants were colchicine treated to double their chromosome number.Chromosomes are genetic carriers that determine the individual traits of organisms.The doubling of chromosomes will lead to modifications in plant morphology,physiology and genetics.To determine the response of mulberry trees induced by colchicine,using mulberry variety Yu-711 leaves as research materials,two small RNA libraries(control and experimental groups)were constructed.It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results.A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA genes,19 genes of which were up-regulated and 18 genes of which were down-regulated.Eight miRNAs were selected from 37 differentially expressed miRNA genes.These miRNAs were verified by quantitative real-time PCR,and the expression levels of these miRNAs were found to be consistent with those obtained by sequencing,which proved the accuracy of sequencing results.The PsRNATarget software was used to predict the target genes of 8 miRNAs.The Gene Ontology and Kyoto Encycopedia of Genes and Genomes analysis were used to collect the functions of target genes and confirm that target genes are mainly involved in biological processes,cell components and molecular functions.novel-77 miRNA was selected from 37 differentially expressed miRNAs.What will serve as a fundamental data in understanding mulberry miRNAs function in molecular biology research and further provides the molecular mechanism of mulberry gene regulation as induced by colchicine is that the prediction of novel_77 precursor sequence and the target gene(XM_010104258.2),as well as the secondary structure analysis.
文摘Acute and recurring pericarditis are frequently encountered clinical entities.Given that severe complications such as tamponade and constrictive pericarditis occur rarely,the majority of patients suffering from acute pericarditis will have a benign clinical course.However,pericarditis recurrence,with its painful symptoms,is frequent.In effect,recent studies have demonstrated a beneficial role of colchicine in preventing recurrence,while also suggesting an increase in recurrences with the use of corticosteroids,the traditional first-line agent.
基金Supported by the MMH grant from Mackay Memorial Hospital, No. 9252
文摘AIM： We studied the effect of colchicine combined with radiation on the survival of human hepatocellular carcinoma （HCC） HA22T/VGH cells.METHODS： Twenty-four hours after treatment with 0-8 ng/mL colchicine, HA22T/VGH cells were irradiated at various doses （0, 1, 2, 4, and 8 Gy）. Colony assay was performed to assess the surviving cell fraction. Survival curves were fitted by using a linear-quadratic model to estimate the sensitizer enhancement ratio （SER）. Flow cytometry was used for cell cycle analysis.RESULTS： Colchicine at lower concentrations （1 and 2 ng/mL） had obvious synergy with radiation to inhibit HCC cell growth, whereas higher concentrations （4 and 8 ng/mL） had only additive effect to radiation. Pretreatment with 1 and 2 ng/mL colchicine for 24-h enhanced cell killing by radiation with SERs of 1.21 and 1.53, respectively.G2/N arrest was only observed with higher colchicine doses （8 and 16 ng/mL） after 24-h treatment, this effect was neither seen with lower doses （1, 2, and 4 ng/mL）nor with any dose after only 1 h of treatment.CONCLUSION： Our results suggest that colchicine has potential as an adjunct to radiotherapy for HCC treatment.Lower doses of colchicine possess radiosensitizing effects via some mechanism other than G2/M arrest. Further study is necessary to elucidate the mechanism.
基金Supported by a Grant fromthe Science and Technology Committee ofGuangdong Province (2003B31801)
文摘Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of eolehicine on HSCs growth were measured by MTT assay. Effects of eolchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide mieroarray. Colehieine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colehicine, the expression of tissue inhibitor of metalloprotenase-1 （TIMP-1） and the expression of interleukin-6 （IL-6） in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchieine＇s beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down regulation of the expression of both TIMP1 and IL-6 in HSCs.
文摘An induced polyploid plant through colchicine treatment offers probably the best scope for improvement in flower size and fruit weight.Thus,in the present study,an attempt was made to induce polyploidy in Cape gooseberry using colchicine with the objective of creating more genetic variability.The colchicine concentrations were used as 0.10%(C1),0.20%(C2)and 0.40%(C3)for the duration 12(H1),24(H2)and 36(H3)hours for each concentration with seedling apex dip method(M1),cotton plug method(M2)and lanolin paste method(M3).The plants treated with 0.10%of colchicine by cotton plug method for 12 h showed the better performance during the years 2017-2018 and 2018-2019 in respect of more delay in the flower bud emergence(54 d and 53 d from the date of transplanting),anthesis(19 d and 20 d from the first appearance of bud to full anthesis of flower)and fruit setting(8.00 d and 9.00 d from the date of anthesis),bigger flower size(2.93 cm2 and 3.00 cm2)than the untreated plants.The lower percentage of pollen viability(40.33%and 40.67%)was noticed in the same treatment in comparison to control(70.33%and 72.33%).The fruit maturity was also extended(59 d and 60 d from the date of fruit set)with bigger sized fruits(length:2.53 cm and 2.57 cm,breadth:2.27 cm and 2.33 cm)as well as more fruit weight(8.70 g and 8.33 g)by the application of colchicine at 0.10%with cotton plug method for 12 h.On the basis of results obtained,the application of colchicine at 0.10%for 12 h duration with cotton plug method was found to be the best and effective treatment for induction of polyploidy as well as more flower size and fruit weight in Cape gooseberry.
文摘The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for their production by in vitro plant cell and tissue cultures have been accelerated considerably. Colchicine, the principle secondary metabolite of Colchicum autumnale L. and Gloriosa superba L., is an important alkaloid that has poison effect used for treatment of various diseases and plant breeding studies. Presently, colchicine has been produced by using the seeds of C. autumnale L. and the tubers of G. superba L. through different chemical extraction methods. Applying in vitro plant cell and tissue cultures together with metabolic and genetic engineering techniques, large-scale production of colchicine can be achieved from the above two plant species.
基金This work was aupported by the Foundation for Scientific Research of Shandong Province, PRC.
文摘The distribution of mitochondria during early development of mouse embryos was visualized bymitochondria-specific vital fluorescent dye, rhodamine 123(Rh 123). Mitochondrial clusters wasmarkedly conceotrated to perinuclear area in blastomere of normal 2-ccll embryos. In blastomere ofuncompacted 8-cell embryos, mitochondria were randomly distributed throughout the cytoplasm, butthey were reorganizcd to the cytocortices beneath the apposed surfaces of blastomere duringcompaction. As demonstrated in our study, colchicine (10 μg/ml) produced marked effect onmitochondrial distribution in blastomcre of 2-cell and compacted 8-cell embryos: mitochondriabecame scattered throughout the cytoplasm ofblastomere. It is suggested that the spatial distributionof mitochondria in early mouse embryo are maintained by microtubule.
基金The Project Supported by National Natural Science Foundation of China
文摘In order to avoid any possible effect of separating procedures from intact cell on morphologic structure of the chromosomes, cultivated Hep-2 tumor cells were treated with colchicine and observed by soft X-ray contact microscopy for the first time. The fine structures of chromosomes are more clear with stereo features. The thread-like and coarse granular structures twine and tangle up together within chromosome masses which can not easily be revealed in Wright’s stained sample by light microscope or osmium stained sample by transmission electron
基金Supported by the Natural Science Fund of Anhui University of Chinese Medicine (No.2010zr011B)the Natural Science Fund of Education Department of Anhui Province,China (No.KJ2011A191)
文摘OBJECTIVE： To establish and optimize the propaga- tion of Nianmaohuangqin （Radix Scutellariae Viscid- ulae） and induce and characterize polyploidy of Nianmaohuangqin （Radix Scutellariae Viscidulae）. METHODS： Buds from germinating seed-derived explants were induced by tissue culture. With an or- thogonal test, different concentrations of 6-benzyl- aminopurine （BAP）, indole-3-acetic acid （IAA） and kinetin （KT） were used to determine the optimal concentrations for the propagation of Nianmaohuangqin （Radix Scutellariae Viscidulae）. The differ- ent concentrations of IAA and rooting powder （ABT） were used to induce rooting. A 0.3% w/v col- chicine solution was used to induce polyploidy and the induced buds was identified by root-tip chromosome determination and stomatal apparatus ob- servation. RESULTS： A large number of buds could be in- duced directly from epicotyl and hypocotyl ex- plants on Murashige and Skoog （MS） （Murashige and Skoog 1962） medium supplemented with 1.1-1.3 mg/L BAP and 0.2 mg/L IAA. Root induction and development could be observed within 20 days of inoculation on 1/2 MS medium supplemented with 0.2 mg/L IAA and 0.1 mg/L ABTo Furthermore, 27 lines of autotetraploid individuals were ob- tained with a plantlet chromosome number of 2n= 4x=36. CONCLUSION： Autotetraploid lines could be ob- tained through induction with colchicine in vitro, proving that this method might be used for plant selection and breeding.
文摘Colchicine has been widely used as an anti-gout medication over the past decades.However,it is less commonly used due to its narrow therapeutic range,meaning that its lethal dose is close to its therapeutic dose.The lethal dose of colchicine is considered to be 0.8 mg/kg.As chronic colchicine poisoning has multiple manifestations,it poses a challenge in the clinician’s differential diagnosis.Historically,the drug was important in treating gout;however,clinical studies are currently underway regarding the use of colchicine in patients with coronavirus disease 2019 as well as its use in coronary artery disease,making this drug more important in clinical practice.CASE SUMMARY A 61-year-old male with a history of gout and chronic colchicine intake was admitted to our Emergency Department due to numbness and weakness of the lower limbs.The patient reported a history of colchicine intake for 23 years.After thorough examination,he was diagnosed with colchicine poisoning,manifesting as neuromyopathy,multiple gastric ulcers and myelosuppression.We advised him to stop taking colchicine and drinking alcohol.We also provided a prescription of lansoprazole and mecobalamin,and then asked him to return to the clinic for re-examination.The patient was followed up for 3-mo during which time his gout symptoms were controlled to the point where he was asymptomatic.CONCLUSION Colchicine overdose can mimic the clinical manifestations of several conditions.Physicians easily pay attention to the disease while ignoring the cause of the disease.Thus,the patient’s medication history should never be ignored.
文摘Objective: Colchicine induced a non-protective Th2-like immunity in Aggregatibacter actinomycetemcomitans-stimulated murine immune response. The aim of the present study was to determine whether colchicine affects inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) production in A. actinomycetemcomitans-immunized mice. Materials and Methods: BALB/c mice were sham-immunized (group I) or immunized with heat-killed A. actinomycetemcomitans (group II-VII). Colchicine was injected intraperitoneally before (group III), on the same day of (group IV), or after (group V) the primary immunization and on the same day of (group VI) or after (group VII) the secondary immunization. In vitro, spleen cells from either sham- or heat-killed A. actinomycetemcomitan-immunized animals were cultured and stimulated with heat-killed A. actinomycetemcomitans in the presence or absence of colchicine with or without addition of L-arginine, Db-cAMP, forskolin or interferon-γ (IFN-γ). The levels of splenic iNOS activity and both serum and culture supernatant NO levels were assessed. Results: The results showed that colchicine did inhibit both splenic iNOS activity and serum NO levels only when the drug was injected at the same time as the immunization (group IV and VI). Splenic iNOS activity and NO levels on antigen-stimulated spleen cell cultures were also suppressed by colchicine, even in the presence of L-arginine, Db-AMP or forskolin. IFN-γ only partially restored iNOS activity and NO levels in the antigen and colchicine-treated spleen cell cultures. Conclusion: This study suggests, therefore, that colchicine may suppress the iNOS activity and NO production in A. actinomycetemcomitans-immunized mice in vivo and in vitro.
