New Zealand (NZ) young rabbits with the administration of insulin-like growth factor (IGF-1) and transforming growth factor-β (TGF-β) with and without mandibular anterior repositioning appliances are explored for th...New Zealand (NZ) young rabbits with the administration of insulin-like growth factor (IGF-1) and transforming growth factor-β (TGF-β) with and without mandibular anterior repositioning appliances are explored for the growth of the mandibular condylar cartilage (MCC). 32 growing NZ and rabbits were divided into 4 groups: the group with saline injection in TMJ, the group which received growth factor injection in TMJ, the group which received anterior positioning appliance and the group which received growth factors injection as well as mandibular repositioning appliance. Gene expression was studied by real-time RT-PCR and cartilage growth by histomorphometry. Administration of growth factors along with mandibular repositioning appliances has induced 1) 1.70-fold expression of Col-2Agene (p value < 0.0005) and 2) 1.47-fold expression of Col-10Agene (p value < 0.0005). In contrast, administration of only mandibular repositioning appliances induced 1) 1.28-fold expression of Col-2Agene (p value < 0.0005) and 2) merely 0.62-fold expression of Col-10Agene (p value < 0.0005), while administration of growth factors only induced 1) mere 0.56-fold expression of Col-2Agene (p value 10A gene (p value growth factors along with mandibular repositioning appliances causes an increase in genetic expressions which have been corroborated by histomorphometry and validated by statistical analysis, during an accelerated growth of mandibular condylar cartilage. Administration of growth factors in the TMJ could provide a synergistic role along with mandibular repositioning appliances for treatment of mandibular retrognathism as well as disorders on the MCC.展开更多
文摘New Zealand (NZ) young rabbits with the administration of insulin-like growth factor (IGF-1) and transforming growth factor-β (TGF-β) with and without mandibular anterior repositioning appliances are explored for the growth of the mandibular condylar cartilage (MCC). 32 growing NZ and rabbits were divided into 4 groups: the group with saline injection in TMJ, the group which received growth factor injection in TMJ, the group which received anterior positioning appliance and the group which received growth factors injection as well as mandibular repositioning appliance. Gene expression was studied by real-time RT-PCR and cartilage growth by histomorphometry. Administration of growth factors along with mandibular repositioning appliances has induced 1) 1.70-fold expression of Col-2Agene (p value < 0.0005) and 2) 1.47-fold expression of Col-10Agene (p value < 0.0005). In contrast, administration of only mandibular repositioning appliances induced 1) 1.28-fold expression of Col-2Agene (p value < 0.0005) and 2) merely 0.62-fold expression of Col-10Agene (p value < 0.0005), while administration of growth factors only induced 1) mere 0.56-fold expression of Col-2Agene (p value 10A gene (p value growth factors along with mandibular repositioning appliances causes an increase in genetic expressions which have been corroborated by histomorphometry and validated by statistical analysis, during an accelerated growth of mandibular condylar cartilage. Administration of growth factors in the TMJ could provide a synergistic role along with mandibular repositioning appliances for treatment of mandibular retrognathism as well as disorders on the MCC.
文摘目的 观察全杜仲胶囊对犬股骨头骨组织中的变化及对转录因子(RUNX2)、1型胶原蛋白(COL-1)蛋白表达的影响,探讨其修复股骨头坏死的机制。方法 选取健康成年比格犬(雌雄各6只,共12只,分笼饲养),随机数字表法分为正常组、模型组、全杜仲胶囊组、仙灵骨葆胶囊组,每组3只。除正常组外,其余3组均采用液氮冷冻法制备双侧股骨头坏死模型。术后第2天开始药物干预,正常组等量生理盐水灌胃,连续12周。观察犬的一般情况,苏木精-伊红染色法(HE)观察犬股骨头组织形态学改变;实时逆转录PCR(Real time RT-PCR)检测RUNX2、COL-1mRNA表达;免疫组化法检测RUNX2、COL-1蛋白表达。结果 与模型组相比较,HE病理染色方面,全杜仲组及仙灵骨葆胶囊组骨小梁面积、骨小梁体积有所恢复;免疫组化方面,与模型组相比,全杜仲胶囊组、仙灵骨葆胶囊组RUNX2蛋白和COL-1蛋白表达上升(P<0.05);Real time RT-PCR检测结果显示RUNX2、COL-1mRNA表达上升(P<0.05)。结论 全杜仲胶囊可以改善犬股骨头坏死组织的变化,这可能是通过调节COL-1及RUNX2蛋白及mRNA的表达调节骨代谢,进而达到防治股骨头坏死的目的。
