The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which ...The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which may be attributed to the fact that small starch particles increase the filling capacity within the dough matrix.This increase subsequently resulted in more complete and uniform structure of dough.Moreover,setback viscosity rose to 1095 cP.The rapid water absorption of coix seed starch led to changes in the secondary structure of proteins in dough.The proportion ofβ-sheet markedly increased,whereas the proportion ofα-helix decreased with increasing level of coix seed flour substitution.Meanwhile,coix seed flour substitution significantly reduced the estimated glycemic index of noodles.Correlation analysis revealed that pasting parameters had significant effects on the evaluation of noodle quality.This study provides a foundation for researchers to substitute wheat flour with coix seed flour.展开更多
Objective:To investigate the therapeutic effects and safety of Dioscorea Decoction combined with Coix Seed in treating cancer cachexia(spleen-kidney Yang deficiency syndrome)in malignant tumor patients.Methods:A total...Objective:To investigate the therapeutic effects and safety of Dioscorea Decoction combined with Coix Seed in treating cancer cachexia(spleen-kidney Yang deficiency syndrome)in malignant tumor patients.Methods:A total of 90 patients with cancer cachexia admitted between June 2024 and June 2025 were randomly divided into a control group and a study group(45 cases each)using a random number generator.Both groups received antitumor therapy,oral megestrol acetate capsules,and conventional nutritional intervention.The study group additionally received oral Dioscorea Decoction combined with Coix Seed.Differences in TCM syndrome scores,nutritional indicators(serum albumin,hemoglobin),and adverse reactions were compared before and after treatment.Results:Baseline TCM syndrome scores and nutritional indicators were comparable between the two groups before treatment.After treatment,both groups showed significant reductions in TCM syndrome scores and increases in serum albumin and hemoglobin levels.The study group exhibited lower TCM syndrome scores and higher serum albumin and hemoglobin levels than the control group,with statistically significant differences.No significant difference in adverse reaction rates was observed between the two groups.Conclusion:Dioscorea Decoction combined with Coix Seed can further improve nutritional status,alleviate clinical symptoms,and demonstrate good safety in treating cancer cachexia patients.展开更多
Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel ...Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel chemotherapy drugs that combine traditional Chinese medicine(TCM)with herbal extracts exhibit superior comprehensive benefits.Herein,we delved into an article authored by Wang et al,focusing specifically on the pharmacological effects of"Herba Patriniae and Coix seed(HC)"and their targeted mechanisms in combating CRC.From the perspective of TCM philosophy,damp-heat stagnation and toxicity are the cardinal pathogenic factors underlying CRC.HC,renowned for their abilities to antipyretic and enhance diuresis,have demonstrated promising efficacy in preliminary studies for the treatment of CRC.These findings offer potential insights in favor of fostering anti-cancer medications.展开更多
BACKGROUND Herba Patriniae and Coix seed(HC)constitute a widely utilized drug combination in the clinical management of colorectal cancer(CRC)that is known for its diuretic,anti-inflammatory,and swelling-reducing prop...BACKGROUND Herba Patriniae and Coix seed(HC)constitute a widely utilized drug combination in the clinical management of colorectal cancer(CRC)that is known for its diuretic,anti-inflammatory,and swelling-reducing properties.Although its efficacy has been demonstrated in a clinical setting,the active compounds and their mechanisms of action in CRC treatment remain to be fully elucidated.AIM To identify the active,CRC-targeting components of HC and to elucidate the mechanisms of action involved.METHODS Active HC components were identified and screened using databases.Targets for each component were predicted.CRC-related targets were obtained from human gene databases.Interaction targets between HC and CRC were identified.A“drug-ingredient-target”network was created to identify the core components and targets involved.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were conducted to elucidate the key pathways involved.Molecular docking between core targets and key components was executed.In vitro experiments validated core monomers.RESULTS Nineteen active components of HC were identified,with acacetin as the primary active compound.The predictive analysis identified 454 targets of the active compounds in HC.Intersection mapping with 2685 CRC-related targets yielded 171 intervention targets,including 30 core targets.GO and KEGG analyses indicated that HC may influence the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway.Molecular docking showed that acacetin exhibited an optimal interaction with AKT1,identifying PI3K,AKT,and P53 as key genes likely targeted by HC during CRC treatment.Acacetin inhibited HT-29 cell proliferation and migration,as well as promoted apoptosis,in vitro.Western blotting analysis revealed increased p53 and cleaved caspase-3 expression and decreased levels of p-PI3K,p-Akt,and survivin,which likely contributed to CRC apoptosis.CONCLUSION Acacetin,the principal active compound in the HC pair,inhibited the proliferation and migration of HT-29 cells and promoted apoptosis through the PI3K/Akt/p53 signaling pathway.展开更多
The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied....The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied.The results revealed that,in Summer and Autumn,especially in August,C.lacryma-jobi could obtain higher biomass and N,P accumulations,thereby achieving the most efficient purification; from the breeding value point of view,N,P contents could be improved by the appropriate increase of harvest times of C.lacryma-jobi,while too many harvest times was inappropriate to the wastewater purification by C.lacryma-jobi and the nutrients accumulation.So,C.lacryma-jobi should be harvested completely in August during its growth period,and twice-3 times of harvests was appropriate.展开更多
Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It ...Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It has the effects of promoting diuresis and dampness,strengthening the spleen to prevent diarrhea,removing arthralgia,expelling pus,and detoxifying and dispersing nodules.It is used for the treatment of edema,athlete's foot,poor urination,spleen deficiency and diarrhea,dampness and obstruction,lung carbuncle,intestinal carbuncle,verruca,and cancer.The medicinal and health value is high,and it has been included in the list of medicinal and food sources in China,which has a large development and application space.This article reviews the current research achievements in the processing methods and anti-tumor activities of Coix seed and provides examples of its clinical application in ancient and modern times,aiming to provide reference for further research on Coix seed and contribute to its clinical application and development.Through the analysis of the traditional Chinese patent medicines,and simple preparations and related health food of Coix seed queried by Yaozhi.com,the source,function,and dosage form of Coix seed were comprehensively analyzed,with a view of providing a reference for the development of Coix seed medicine and food.展开更多
Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to th...Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.展开更多
[Objectives] To screen and analyze germplasm resources of wild Coix lacryma-jobi L. with high content of coixin. [Methods] From the 150 kinds of collected wild C. lacryma-jobi L. germplasm resources, 24 kinds of excel...[Objectives] To screen and analyze germplasm resources of wild Coix lacryma-jobi L. with high content of coixin. [Methods] From the 150 kinds of collected wild C. lacryma-jobi L. germplasm resources, 24 kinds of excellent resources were screened and detected. Morphological statistics were carried out, and the content of coixin in the four organ of roots, stems, leaves and seeds were measured, and the approved cultivar white husk Liugu of C. lacryma-jobi L. was taking as the control. [Results] Among the organs of the plant of C. lacryma-jobi L., the roots had the highest content of coixin, so the roots of C. lacryma-jobi L. can be used as the medicinal parts of C. lacryma-jobi L. [Conclusions] The wild C. lacryma-jobi L.Y229, Y164, Y166, Y83, and Y84 stains had excellent yield, and their root coixin content was significantly higher than that of the control variety, so they can be used for cultivation as excellent germplasm, so as to obtain new variety(line) of C. lacryma-jobi L.with higher content of coixin.展开更多
In order to study the effects of drought stress on the morphological and physiological indexes of Yunnan Coix at different growth stages, two new varieties of Yunnan Coix Wenyi 4 and Wenyi 5 were used as experimental ...In order to study the effects of drought stress on the morphological and physiological indexes of Yunnan Coix at different growth stages, two new varieties of Yunnan Coix Wenyi 4 and Wenyi 5 were used as experimental materials, potted and compared with CK by normal irrigation. Morphological indices such as plant height, stem diameter and physiological indices such as protein, soluble sugar, chlorophyll content and relative conductivity (REC) of the two varieties under drought stress at seedling stage, tillering stage and jointing stage were determined, and data statistics and variance analysis were performed for each index. The results showed that the morphological indices, plant height of Wenyi 4 and Wenyi 5 were significantly affected by drought stress at seedling stage, while the stem diameter was significantly affected by drought stress at seedling and jointing stage. In terms of physiological indices, the relative conductivity, protein and soluble sugar contents of Wenyi 5 increased at a higher degree, whereas chlorophyll content decreased at a higher degree, indicating that Wenyi 5 was greatly affected by drought stress. Through the analysis of drought resistance of the tested materials at different stages by polar ordination method, the results were as follows: jointing stage of Wenyi 5>jointing stage of Wenyi 4>seedling stage of Wenyi 4>seedling stage of Wenyi 5>tillering Stage of Wenyi 4>Tillering Stage of Wenyi 5. For the growth period, the drought resistance was as follows: jointing stage>seedling stage>tillering stage. In conclusion, Wenyi 4 has strong drought resistance and is suitable for droughtresistant cultivation.展开更多
In order to study high yield cultivation technology for Coix Lachryrma-jobi L.,and discuss the effect of planting density,fertilizer level,and number of seedlings per hole on yield of Coix Lachryrma-jobi L.,this paper...In order to study high yield cultivation technology for Coix Lachryrma-jobi L.,and discuss the effect of planting density,fertilizer level,and number of seedlings per hole on yield of Coix Lachryrma-jobi L.,this paper took domesticated variety Xingren Small White Shell of Coix Lachryrma-jobi L. as an example. Using L9( 34) orthogonal design,it selected optimum combination of planting density,fertilizer level,and number of seedlings per hole to guide planting of Coix Lachryrma-jobi L.. Experiment indicated that the optimum planting density( spacing in the rows and spacing between rows) of Coix Lachryrma-jobi L. is 40 cm x 80 cm; the optimum fertilizer level is pure nitrogen 15 kg,pure phosphorus 15 kg and pure potassium 15 kg per mu; the optimum number of seedlings per hole is 5 seedlings per hole. Through variance analysis,it indicated that planting density exerts significance effect on the yield of Coix Lachryrma-jobi L.展开更多
The physicochemical properties and composition of coix seed oil produced by Monascus purpureus fermentation and supercritical CO_(2)extraction were determined.Anti-lipid-oxidation and edible safety were evaluated usin...The physicochemical properties and composition of coix seed oil produced by Monascus purpureus fermentation and supercritical CO_(2)extraction were determined.Anti-lipid-oxidation and edible safety were evaluated using a cholesterol-fish oil model,acute oral toxicity assay,and genetic toxicity assay in vitro and in vivo,respectively.The results show that the extraction oil from fermented coix seed(FCS-O)had good physicochemical quality and abundant active components with physiological function.In particular,γ-tocotrienol,γ-oryzanol,coixenolide and oleic acid concentrations reached 72.83μg/g,745.96μg/g,9.65 mg/g and 316.58 mg/100 g DW,respectively.The FCS-O exhibited higher antioxidant capability in inhibiting lipid oxidation and peroxidation.Compared to the blank control,the concentrations of 7-ketocholestreol and peroxide only were 8.42μg/mL and 16.16 mmol/kg at 168 h of oxidation(P<0.01).In addition,the FCS-O has been confirmed to be a very safe edible oil,with no acute toxicity(LD50>10 g/kg bw,considered actually non-toxic)and no induced mutagenicity,cytotoxicity or genotoxicity.These results serve as a good safety reference for future application of the oil from fermented coix seed.The development and utilization of this kind of oil will be beneficial as a food,food ingredient,nutritional supplement,or natural food antioxidant to promote good health function.展开更多
A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique includ...A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique including genomic in situ hybridization(GISH).This new C.aquatica cyto-type was different from other C.aquatica cyto-types reported in chromosome structure.It was 2n=30 in chromosome number,and ten bivalents plus ten univalents were formed in meiotic prophase and metaphase I.GISH results indicated that its 20 chromosomes were highly homologous to chromosomes of allotetraploid species C.lacryma-jobi L.(2n=20).The 45S and 5S rDNA were respectively located on the two different chromosomes of the C.aquatica.One of chromosomes carrying 45S rDNA showed its chromosome shape and signal location in the C.aquatica as the same as that in C.lacryma-jobi.So did one of chromosomes carrying 5S rDNA.It could be deducted that one of parental candidates for the new C.aquatica cyto-type may be C.lacryma-jobi The other parental candidate may be allooctopolyploid C.aquatica(2n=40),which is similar to the new cyto-type of C.aquatica in aquatic habit and plant morphology.展开更多
Coix seed is a dry mature seed of Gramineae plant Coix. It has been confirmed that a plurality of components thereof have pharmacological effects. The mechanism of Coix seed against tumor includes the following aspect...Coix seed is a dry mature seed of Gramineae plant Coix. It has been confirmed that a plurality of components thereof have pharmacological effects. The mechanism of Coix seed against tumor includes the following aspects:(1) Inhibit the proliferation of tumor cells.(2) Inhibit the invasion and metastasis of tumor cells.(3) Inhibit the formation of tumor blood vessels.(4) Induce apoptosis of tumor cells.(5) The synergistic and attenuating effects on chemotherapy.(6) Enhance the sensitivity of tumor cells to radiation.(7) Adjust the body's immune function.(8) Relieve the pain of advanced tumors. In this paper, we summarized the research adcances of Coix seed foucusing on its anti-tumor effects.展开更多
[Objectives] To extract the flavonoids from leaves of Coix lacryma-jobi L. [Methods] Ethanol extraction method was adopted,spectrophotometry was used,and single factor experiment and orthogonal experiment were carried...[Objectives] To extract the flavonoids from leaves of Coix lacryma-jobi L. [Methods] Ethanol extraction method was adopted,spectrophotometry was used,and single factor experiment and orthogonal experiment were carried out to study the effects of ethanol percentage,extraction temperature,solid-to-liquid ratio and extraction time on the extraction of total flavonoids from leaves of C. lacryma-jobi L.[Results] The order of 4 factors influencing the extraction of flavonoids from leaves of C. lacryma-jobi L. was: solid-to-liquid ratio > extraction time > ethanol percentage > extraction temperature. When the extraction temperature was 70℃,the extraction time was 1. 5 h and the solid-liquid ratio was 1: 10,the ethanol percentage was 60%,the extraction effect was the best,extraction of flavonoids was 0. 107 5 mg/m L.[Conclusions] This study is expected to provide a theoretical basis for further development and utilization of C. lacryma-jobi L.展开更多
[Objectives]The paper was to study the effects of different herbicides on weed control,agronomic characters and grain quality of Coix lacryma-jobi L.[Methods]Six commonly used herbicides were selected for the trial,in...[Objectives]The paper was to study the effects of different herbicides on weed control,agronomic characters and grain quality of Coix lacryma-jobi L.[Methods]Six commonly used herbicides were selected for the trial,including atrazine SC,butachlor SC,monosulfuron WP,MCPA-starane EC,clopyralid AS and tribenuron-methyl WP.[Results](i)Atrazine,butachlor and monosulfuron had no chemical injury to C.lacryma-jobi L.,while clopyralid caused slight chemical injury.(ii)The fresh weight control effects of atrazine,butachlor and monosulfuron on weeds increased first and then decreased.The initial control effect of MCPA-starane and clopyralid reached more than 90%;the control effect of monosulfuron increased gradually in the late stage,reaching 97%at 60 d post spraying,and the overall control effect was optimal.(iii)Compared with manual weeding,herbicides had no significant effect on plant height of C.lacryma-jobi L.However,compared with no weeding,other herbicides except tribenuron-methyl inhibited plant growth to a certain extent.Herbicides had different effects on main stem diameter,tiller number per plant and grain number per plant of C.lacryma-jobi L.(iv)Different herbicides had no significant effect on 100-grain weight,but had significant effect on yield.(v)Compared with manual weeding,atrazine,butachlor and clopyralid decreased the protein content of C.lacryma-jobi L.,and the use of atrazine,butachlor,monosulfuron,MCPA-starane and tribenuron-methyl decreased the fat content of C.lacryma-jobi L.;butachlor and MCPA-starane decreased the glycerol trioleate content of C.lacryma-jobi L.Compared with no weeding,chemical herbicides had no significant effect on the protein content,while atrazine decreased the fat content,and butachlor and MCPA-starane decreased the glycerol trioleate content of C.lacryma-jobi L.[Conclusions]The use of herbicides significantly increases crop yield compared with no weeding,but has an impact on agronomic characters and grain quality.展开更多
Levels of T1 （Thallium） in soil from 0 （control） to 50 μg/L through 0.2, 0.5, 1 and 2.5μg/L were directly and positively correlated to levels of T1 in plant tissue, the accumulation being maximum in roots, inter...Levels of T1 （Thallium） in soil from 0 （control） to 50 μg/L through 0.2, 0.5, 1 and 2.5μg/L were directly and positively correlated to levels of T1 in plant tissue, the accumulation being maximum in roots, intermediate in leaves and minimum in stems. Thallium, especially at higher concentrations, adversely affected photosynthesis （as judged based on chlorophyll fluorescence parameters）, suggesting inhibition of photo-activation of PSII （Photosystems II）, and also decreased the rate of photosynthesis, the rate of transpiration and stomatal conductivity drastically. Exposure to TI also increased the activity of CAT （Catalase） （except at 1 μg/L） and POD （Peroxidase） （except at 0.2 μg/L）, suggesting that the antioxidant systems in Coix lacryma-jobi were the main contributors of CAT and SOD （Superoxide Dismutase） and that the tolerance of C. lacryma-jobi to T1 is mainly due to this induced antioxidant machinery.展开更多
Objective:Study on the mechanism of Coix seed in the treatment of colon cancer based on network pharmacology.Methods:The main chemical components of Coix seed were identified by TCMP,and the target sites of drug compo...Objective:Study on the mechanism of Coix seed in the treatment of colon cancer based on network pharmacology.Methods:The main chemical components of Coix seed were identified by TCMP,and the target sites of drug components were screened.The target of the protein was mapped to the corresponding gene target through the Uniprot database.Genecards database was used to find the target gene of human colon cancer disease,and then the disease gene target was combined with the drug gene target to screen the common target of disease-drug composition,and the STRING platform was used to construct the drug component-disease target protein interaction.(PPI)network model.The Bioconductor database was used to analyze the potential targets,related molecular functions and signaling pathways of cocoon in the treatment of colon cancer by GO and KEGG enrichment.The disease-drug component-target-signal pathway network was established by Cytoscape software.Results:It was found that there are 9 pharmacodynamics and 28 targets related to colon cancer treatment,and GO functional enrichment analysis is based on correction.P<0.05 screened 346 molecular functional related items,including 256 biological processes,48 molecular functions,and 42 cell components.KEGG functional enrichment analysis screened 24 related pathways according to the adjusted P<0.05.Conclusion:PTGS2,AR,ESR1,PGR,ADH1C,AKR1B1,LTA4H,NCOA1,NCOA2 and other related targets may be potential targets for the treatment of colon cancer.展开更多
文摘The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which may be attributed to the fact that small starch particles increase the filling capacity within the dough matrix.This increase subsequently resulted in more complete and uniform structure of dough.Moreover,setback viscosity rose to 1095 cP.The rapid water absorption of coix seed starch led to changes in the secondary structure of proteins in dough.The proportion ofβ-sheet markedly increased,whereas the proportion ofα-helix decreased with increasing level of coix seed flour substitution.Meanwhile,coix seed flour substitution significantly reduced the estimated glycemic index of noodles.Correlation analysis revealed that pasting parameters had significant effects on the evaluation of noodle quality.This study provides a foundation for researchers to substitute wheat flour with coix seed flour.
文摘Objective:To investigate the therapeutic effects and safety of Dioscorea Decoction combined with Coix Seed in treating cancer cachexia(spleen-kidney Yang deficiency syndrome)in malignant tumor patients.Methods:A total of 90 patients with cancer cachexia admitted between June 2024 and June 2025 were randomly divided into a control group and a study group(45 cases each)using a random number generator.Both groups received antitumor therapy,oral megestrol acetate capsules,and conventional nutritional intervention.The study group additionally received oral Dioscorea Decoction combined with Coix Seed.Differences in TCM syndrome scores,nutritional indicators(serum albumin,hemoglobin),and adverse reactions were compared before and after treatment.Results:Baseline TCM syndrome scores and nutritional indicators were comparable between the two groups before treatment.After treatment,both groups showed significant reductions in TCM syndrome scores and increases in serum albumin and hemoglobin levels.The study group exhibited lower TCM syndrome scores and higher serum albumin and hemoglobin levels than the control group,with statistically significant differences.No significant difference in adverse reaction rates was observed between the two groups.Conclusion:Dioscorea Decoction combined with Coix Seed can further improve nutritional status,alleviate clinical symptoms,and demonstrate good safety in treating cancer cachexia patients.
基金National Natural Science Foundation of China,No.81970529The Natural Science Foundation of Jilin Province,No.20230508074RC and No.YDZJ202401218ZYTS.
文摘Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel chemotherapy drugs that combine traditional Chinese medicine(TCM)with herbal extracts exhibit superior comprehensive benefits.Herein,we delved into an article authored by Wang et al,focusing specifically on the pharmacological effects of"Herba Patriniae and Coix seed(HC)"and their targeted mechanisms in combating CRC.From the perspective of TCM philosophy,damp-heat stagnation and toxicity are the cardinal pathogenic factors underlying CRC.HC,renowned for their abilities to antipyretic and enhance diuresis,have demonstrated promising efficacy in preliminary studies for the treatment of CRC.These findings offer potential insights in favor of fostering anti-cancer medications.
基金Supported by the National Natural Science Foundation of China,No.82174464China Academy of Traditional Chinese Medicine Science and Technology Innovation Project,No.CI2021A01806Central High Level Traditional Chinese Medicine Hospital Clinical Research and Achievement Transformation Ability Enhancement Project,No.HLCMHPP2023085.
文摘BACKGROUND Herba Patriniae and Coix seed(HC)constitute a widely utilized drug combination in the clinical management of colorectal cancer(CRC)that is known for its diuretic,anti-inflammatory,and swelling-reducing properties.Although its efficacy has been demonstrated in a clinical setting,the active compounds and their mechanisms of action in CRC treatment remain to be fully elucidated.AIM To identify the active,CRC-targeting components of HC and to elucidate the mechanisms of action involved.METHODS Active HC components were identified and screened using databases.Targets for each component were predicted.CRC-related targets were obtained from human gene databases.Interaction targets between HC and CRC were identified.A“drug-ingredient-target”network was created to identify the core components and targets involved.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were conducted to elucidate the key pathways involved.Molecular docking between core targets and key components was executed.In vitro experiments validated core monomers.RESULTS Nineteen active components of HC were identified,with acacetin as the primary active compound.The predictive analysis identified 454 targets of the active compounds in HC.Intersection mapping with 2685 CRC-related targets yielded 171 intervention targets,including 30 core targets.GO and KEGG analyses indicated that HC may influence the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway.Molecular docking showed that acacetin exhibited an optimal interaction with AKT1,identifying PI3K,AKT,and P53 as key genes likely targeted by HC during CRC treatment.Acacetin inhibited HT-29 cell proliferation and migration,as well as promoted apoptosis,in vitro.Western blotting analysis revealed increased p53 and cleaved caspase-3 expression and decreased levels of p-PI3K,p-Akt,and survivin,which likely contributed to CRC apoptosis.CONCLUSION Acacetin,the principal active compound in the HC pair,inhibited the proliferation and migration of HT-29 cells and promoted apoptosis through the PI3K/Akt/p53 signaling pathway.
基金Supported by Project Sponsored by National Natural Science Foundation (30370146)Projects Sponsored by European Union (ERBIC18CT960059)~~
文摘The dynamic of growth and nutrient （N and P） absorption of Coix lacryma-jobi Linn.in constructed wetland （CW）,as well as the effect of constructed wetland harvest on N,P absorption in eutrophic water were studied.The results revealed that,in Summer and Autumn,especially in August,C.lacryma-jobi could obtain higher biomass and N,P accumulations,thereby achieving the most efficient purification; from the breeding value point of view,N,P contents could be improved by the appropriate increase of harvest times of C.lacryma-jobi,while too many harvest times was inappropriate to the wastewater purification by C.lacryma-jobi and the nutrients accumulation.So,C.lacryma-jobi should be harvested completely in August during its growth period,and twice-3 times of harvests was appropriate.
基金Supported by Ningxia Natural Science Foundation,No.2022AAC02039Ningxia Key Research and Development Program,No.2023BEG02015National Natural Science Foundation of China,No.82260879.
文摘Coix seed is a dry and mature seed of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf in the Gramineae family.Coix seed has a sweet,light taste,and a cool nature.Coix seed enters the spleen,stomach,and lung meridians.It has the effects of promoting diuresis and dampness,strengthening the spleen to prevent diarrhea,removing arthralgia,expelling pus,and detoxifying and dispersing nodules.It is used for the treatment of edema,athlete's foot,poor urination,spleen deficiency and diarrhea,dampness and obstruction,lung carbuncle,intestinal carbuncle,verruca,and cancer.The medicinal and health value is high,and it has been included in the list of medicinal and food sources in China,which has a large development and application space.This article reviews the current research achievements in the processing methods and anti-tumor activities of Coix seed and provides examples of its clinical application in ancient and modern times,aiming to provide reference for further research on Coix seed and contribute to its clinical application and development.Through the analysis of the traditional Chinese patent medicines,and simple preparations and related health food of Coix seed queried by Yaozhi.com,the source,function,and dosage form of Coix seed were comprehensively analyzed,with a view of providing a reference for the development of Coix seed medicine and food.
基金Supported by the Special Financial Program of Hebei Province,the Fund for Agricultural Science and Technology Innovation Talent Team Construction(2011055001)~~
文摘Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.
基金Supported by Special Project of Guizhou Academy of Agricultural Sciences(Qian Nong Ke Yuan Yuan Zhuan Xiang[2014]010)Science and Technology Research Project for Social Development of Guizhou Province(Qian Ke He SY Zi[2015]3023-4)+1 种基金Special Project of Guizhou Academy of Agricultural Sciences(Qian Nong Ke Yuan Yuan Zhuan Xiang [2014]032)Project of Talent Work Leading Group Office of Guizhou Province(Qian Ren Ling Fa[2016]22)
文摘[Objectives] To screen and analyze germplasm resources of wild Coix lacryma-jobi L. with high content of coixin. [Methods] From the 150 kinds of collected wild C. lacryma-jobi L. germplasm resources, 24 kinds of excellent resources were screened and detected. Morphological statistics were carried out, and the content of coixin in the four organ of roots, stems, leaves and seeds were measured, and the approved cultivar white husk Liugu of C. lacryma-jobi L. was taking as the control. [Results] Among the organs of the plant of C. lacryma-jobi L., the roots had the highest content of coixin, so the roots of C. lacryma-jobi L. can be used as the medicinal parts of C. lacryma-jobi L. [Conclusions] The wild C. lacryma-jobi L.Y229, Y164, Y166, Y83, and Y84 stains had excellent yield, and their root coixin content was significantly higher than that of the control variety, so they can be used for cultivation as excellent germplasm, so as to obtain new variety(line) of C. lacryma-jobi L.with higher content of coixin.
文摘In order to study the effects of drought stress on the morphological and physiological indexes of Yunnan Coix at different growth stages, two new varieties of Yunnan Coix Wenyi 4 and Wenyi 5 were used as experimental materials, potted and compared with CK by normal irrigation. Morphological indices such as plant height, stem diameter and physiological indices such as protein, soluble sugar, chlorophyll content and relative conductivity (REC) of the two varieties under drought stress at seedling stage, tillering stage and jointing stage were determined, and data statistics and variance analysis were performed for each index. The results showed that the morphological indices, plant height of Wenyi 4 and Wenyi 5 were significantly affected by drought stress at seedling stage, while the stem diameter was significantly affected by drought stress at seedling and jointing stage. In terms of physiological indices, the relative conductivity, protein and soluble sugar contents of Wenyi 5 increased at a higher degree, whereas chlorophyll content decreased at a higher degree, indicating that Wenyi 5 was greatly affected by drought stress. Through the analysis of drought resistance of the tested materials at different stages by polar ordination method, the results were as follows: jointing stage of Wenyi 5>jointing stage of Wenyi 4>seedling stage of Wenyi 4>seedling stage of Wenyi 5>tillering Stage of Wenyi 4>Tillering Stage of Wenyi 5. For the growth period, the drought resistance was as follows: jointing stage>seedling stage>tillering stage. In conclusion, Wenyi 4 has strong drought resistance and is suitable for droughtresistant cultivation.
基金Supported by Key Agricultural Project of Guizhou Province(NY(2011)3093)Guizhou Provincial Science Foundation(J(2013)2192)+1 种基金National Spark Plan Project(2013GA820001)Joint Fund Project of Guizhou Academy of Agricultural Sciences(LH(2014)7692)
文摘In order to study high yield cultivation technology for Coix Lachryrma-jobi L.,and discuss the effect of planting density,fertilizer level,and number of seedlings per hole on yield of Coix Lachryrma-jobi L.,this paper took domesticated variety Xingren Small White Shell of Coix Lachryrma-jobi L. as an example. Using L9( 34) orthogonal design,it selected optimum combination of planting density,fertilizer level,and number of seedlings per hole to guide planting of Coix Lachryrma-jobi L.. Experiment indicated that the optimum planting density( spacing in the rows and spacing between rows) of Coix Lachryrma-jobi L. is 40 cm x 80 cm; the optimum fertilizer level is pure nitrogen 15 kg,pure phosphorus 15 kg and pure potassium 15 kg per mu; the optimum number of seedlings per hole is 5 seedlings per hole. Through variance analysis,it indicated that planting density exerts significance effect on the yield of Coix Lachryrma-jobi L.
基金supported by Natural Science Foundation of China(32260583)the Agriculture Committee of Guizhou Province[(2017)106&(2018)81]the Talent Introduction Program of Guizhou University[(2021)76].
文摘The physicochemical properties and composition of coix seed oil produced by Monascus purpureus fermentation and supercritical CO_(2)extraction were determined.Anti-lipid-oxidation and edible safety were evaluated using a cholesterol-fish oil model,acute oral toxicity assay,and genetic toxicity assay in vitro and in vivo,respectively.The results show that the extraction oil from fermented coix seed(FCS-O)had good physicochemical quality and abundant active components with physiological function.In particular,γ-tocotrienol,γ-oryzanol,coixenolide and oleic acid concentrations reached 72.83μg/g,745.96μg/g,9.65 mg/g and 316.58 mg/100 g DW,respectively.The FCS-O exhibited higher antioxidant capability in inhibiting lipid oxidation and peroxidation.Compared to the blank control,the concentrations of 7-ketocholestreol and peroxide only were 8.42μg/mL and 16.16 mmol/kg at 168 h of oxidation(P<0.01).In addition,the FCS-O has been confirmed to be a very safe edible oil,with no acute toxicity(LD50>10 g/kg bw,considered actually non-toxic)and no induced mutagenicity,cytotoxicity or genotoxicity.These results serve as a good safety reference for future application of the oil from fermented coix seed.The development and utilization of this kind of oil will be beneficial as a food,food ingredient,nutritional supplement,or natural food antioxidant to promote good health function.
文摘A new Coix aquatica Roxb.cyto-type,found in the southwest of Guangxi Zhuang Autonomous Region in China,was identified and analyzed by acetocarmine squashing and fluorescence in situ hybridization(FISH)technique including genomic in situ hybridization(GISH).This new C.aquatica cyto-type was different from other C.aquatica cyto-types reported in chromosome structure.It was 2n=30 in chromosome number,and ten bivalents plus ten univalents were formed in meiotic prophase and metaphase I.GISH results indicated that its 20 chromosomes were highly homologous to chromosomes of allotetraploid species C.lacryma-jobi L.(2n=20).The 45S and 5S rDNA were respectively located on the two different chromosomes of the C.aquatica.One of chromosomes carrying 45S rDNA showed its chromosome shape and signal location in the C.aquatica as the same as that in C.lacryma-jobi.So did one of chromosomes carrying 5S rDNA.It could be deducted that one of parental candidates for the new C.aquatica cyto-type may be C.lacryma-jobi The other parental candidate may be allooctopolyploid C.aquatica(2n=40),which is similar to the new cyto-type of C.aquatica in aquatic habit and plant morphology.
文摘Coix seed is a dry mature seed of Gramineae plant Coix. It has been confirmed that a plurality of components thereof have pharmacological effects. The mechanism of Coix seed against tumor includes the following aspects:(1) Inhibit the proliferation of tumor cells.(2) Inhibit the invasion and metastasis of tumor cells.(3) Inhibit the formation of tumor blood vessels.(4) Induce apoptosis of tumor cells.(5) The synergistic and attenuating effects on chemotherapy.(6) Enhance the sensitivity of tumor cells to radiation.(7) Adjust the body's immune function.(8) Relieve the pain of advanced tumors. In this paper, we summarized the research adcances of Coix seed foucusing on its anti-tumor effects.
基金Supported by Project of National Natural Science Foundation(81360684)Natural Science Foundation Project of Guangxi(2011GXNSFA018046)+3 种基金the 12th Five-Year TCM Key Discipline Chinese Medicine Chemistry Construction Program of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Ren Jiao Fa[2012]32)Key Discipline Chinese Medicine Chemistry Construction Program of Guangxi(Gui Jiao Ke Yan[2013]16)Program of Key Laboratory of Guangxi Universities on National Medicine in Youjiang River Basin(Gui Jiao Ke Yan[2014]14)Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi in 2015(201510599026)
文摘[Objectives] To extract the flavonoids from leaves of Coix lacryma-jobi L. [Methods] Ethanol extraction method was adopted,spectrophotometry was used,and single factor experiment and orthogonal experiment were carried out to study the effects of ethanol percentage,extraction temperature,solid-to-liquid ratio and extraction time on the extraction of total flavonoids from leaves of C. lacryma-jobi L.[Results] The order of 4 factors influencing the extraction of flavonoids from leaves of C. lacryma-jobi L. was: solid-to-liquid ratio > extraction time > ethanol percentage > extraction temperature. When the extraction temperature was 70℃,the extraction time was 1. 5 h and the solid-liquid ratio was 1: 10,the ethanol percentage was 60%,the extraction effect was the best,extraction of flavonoids was 0. 107 5 mg/m L.[Conclusions] This study is expected to provide a theoretical basis for further development and utilization of C. lacryma-jobi L.
基金Key Technology R&D Program of Guizhou Province(QKHZC[2021]247)QKHZC[2018]2310。
文摘[Objectives]The paper was to study the effects of different herbicides on weed control,agronomic characters and grain quality of Coix lacryma-jobi L.[Methods]Six commonly used herbicides were selected for the trial,including atrazine SC,butachlor SC,monosulfuron WP,MCPA-starane EC,clopyralid AS and tribenuron-methyl WP.[Results](i)Atrazine,butachlor and monosulfuron had no chemical injury to C.lacryma-jobi L.,while clopyralid caused slight chemical injury.(ii)The fresh weight control effects of atrazine,butachlor and monosulfuron on weeds increased first and then decreased.The initial control effect of MCPA-starane and clopyralid reached more than 90%;the control effect of monosulfuron increased gradually in the late stage,reaching 97%at 60 d post spraying,and the overall control effect was optimal.(iii)Compared with manual weeding,herbicides had no significant effect on plant height of C.lacryma-jobi L.However,compared with no weeding,other herbicides except tribenuron-methyl inhibited plant growth to a certain extent.Herbicides had different effects on main stem diameter,tiller number per plant and grain number per plant of C.lacryma-jobi L.(iv)Different herbicides had no significant effect on 100-grain weight,but had significant effect on yield.(v)Compared with manual weeding,atrazine,butachlor and clopyralid decreased the protein content of C.lacryma-jobi L.,and the use of atrazine,butachlor,monosulfuron,MCPA-starane and tribenuron-methyl decreased the fat content of C.lacryma-jobi L.;butachlor and MCPA-starane decreased the glycerol trioleate content of C.lacryma-jobi L.Compared with no weeding,chemical herbicides had no significant effect on the protein content,while atrazine decreased the fat content,and butachlor and MCPA-starane decreased the glycerol trioleate content of C.lacryma-jobi L.[Conclusions]The use of herbicides significantly increases crop yield compared with no weeding,but has an impact on agronomic characters and grain quality.
文摘Levels of T1 （Thallium） in soil from 0 （control） to 50 μg/L through 0.2, 0.5, 1 and 2.5μg/L were directly and positively correlated to levels of T1 in plant tissue, the accumulation being maximum in roots, intermediate in leaves and minimum in stems. Thallium, especially at higher concentrations, adversely affected photosynthesis （as judged based on chlorophyll fluorescence parameters）, suggesting inhibition of photo-activation of PSII （Photosystems II）, and also decreased the rate of photosynthesis, the rate of transpiration and stomatal conductivity drastically. Exposure to TI also increased the activity of CAT （Catalase） （except at 1 μg/L） and POD （Peroxidase） （except at 0.2 μg/L）, suggesting that the antioxidant systems in Coix lacryma-jobi were the main contributors of CAT and SOD （Superoxide Dismutase） and that the tolerance of C. lacryma-jobi to T1 is mainly due to this induced antioxidant machinery.
基金This study was supported by National Natural Science Foundation of China(81673862,81660833,81760814)Ministry of Education of Guizhou Province(GZS word[2016]08)+2 种基金Guizhou Provincial Science and Technology Department(Qiankehe Talents(2016)4032)Tumor Inheritance and Scientific and Technological Innovation Talents Base of Guizhou Province(Qianren Leading Development[2018]3)Graduate Student Workstation of Guizhou Province Set up a project(Guizhou Teaching and Research Co-operation JYSZ word[2014]018).
文摘Objective:Study on the mechanism of Coix seed in the treatment of colon cancer based on network pharmacology.Methods:The main chemical components of Coix seed were identified by TCMP,and the target sites of drug components were screened.The target of the protein was mapped to the corresponding gene target through the Uniprot database.Genecards database was used to find the target gene of human colon cancer disease,and then the disease gene target was combined with the drug gene target to screen the common target of disease-drug composition,and the STRING platform was used to construct the drug component-disease target protein interaction.(PPI)network model.The Bioconductor database was used to analyze the potential targets,related molecular functions and signaling pathways of cocoon in the treatment of colon cancer by GO and KEGG enrichment.The disease-drug component-target-signal pathway network was established by Cytoscape software.Results:It was found that there are 9 pharmacodynamics and 28 targets related to colon cancer treatment,and GO functional enrichment analysis is based on correction.P<0.05 screened 346 molecular functional related items,including 256 biological processes,48 molecular functions,and 42 cell components.KEGG functional enrichment analysis screened 24 related pathways according to the adjusted P<0.05.Conclusion:PTGS2,AR,ESR1,PGR,ADH1C,AKR1B1,LTA4H,NCOA1,NCOA2 and other related targets may be potential targets for the treatment of colon cancer.
